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Orthodontic-surgical treatment for severe skeletal class Ⅱ malocclusion with vertical maxillary excess and four premolars extraction: A case report 被引量:2
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作者 Yi-Wen Zhou Yan-Yi Wang +3 位作者 Zhi-Feng He Ming-Xing Lu Gui-Feng Li Huang Li 《World Journal of Clinical Cases》 SCIE 2023年第5期1106-1114,共9页
BACKGROUND Patient satisfaction with facial appearance at the end of orthodontic camouflage treatment is very important, especially for skeletal malocclusion. This case report highlights the importance of the treatmen... BACKGROUND Patient satisfaction with facial appearance at the end of orthodontic camouflage treatment is very important, especially for skeletal malocclusion. This case report highlights the importance of the treatment plan for a patient initially treated with four-premolar-extraction camouflage, despite indications for orthognathic surgery.CASE SUMMARY A 23-year-old male sought treatment complaining about his unsatisfactory facial appearance. His maxillary first premolars and mandibular second premolars had been extracted, and a fixed appliance had been used to retract his anterior teeth for two years without improvement. He had a convex profile, a gummy smile, lip incompetence, inadequate maxillary incisor inclination, and almost a class I molar relationship. Cephalometric analysis showed severe skeletal class Ⅱ malocclusion(A point-nasion-B point = 11.5°) with a retrognathic mandible(sella-nasion-B point = 75.9°), a protruded maxilla(sella-nasion-A point = 87.4°), and vertical maxillary excess(upper incisor to palatal plane = 33.2 mm). The excessive lingual inclination of the maxillary incisors(upper incisor to nasion-A point line =-5.5°)was due to previous treatment attempts to compensate for the skeletal class Ⅱ malocclusion. The patient was successfully retreated with decompensating orthodontic treatment combined with orthognathic surgery. The maxillary incisors were repositioned and proclined in the alveolar bone, the overjet was increased, and a space was created for orthognathic surgery, including maxillary impaction, anterior maxillary back-setting, and bilateral sagittal split ramus osteotomy to correct his skeletal anteroposterior discrepancy. Gingival display was reduced, and lip competence was restored. In addition, the results remained stable after 2 years. The patient was satisfied with his new profile as well as with the functional malocclusion at the end of treatment.CONCLUSION This case report provides orthodontists a good example of how to treat an adult with severe skeletal class Ⅱ malocclusion with vertical maxillary excess after an unsatisfactory orthodontic camouflage treatment. Orthodontic and orthognathic treatment can significantly correct a patient’s facial appearance. 展开更多
关键词 Case report Skeletal classmalocclusion Vertical excess Gummy smile Camouflage treatment Orthognathic surgery
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Prevalence of angle class III malocclusion: A systematic review and meta-analysis 被引量:15
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作者 Daniel K. Hardy Yltze P. Cubas Maria F. Orellana 《Open Journal of Epidemiology》 2012年第4期75-82,共8页
Objective: This study seeks to review current and relevant literature on global Angle class III malocclusion prevalence. Materials and Methods: The electronic databases PubMed, ISI Web of Knowledge, and the Cochrane D... Objective: This study seeks to review current and relevant literature on global Angle class III malocclusion prevalence. Materials and Methods: The electronic databases PubMed, ISI Web of Knowledge, and the Cochrane Database of Systematic Review were searched using specific inclusion criteria to obtain applicable articles. All pertinent references were also examined for acceptability. Results: A total of 20 articles were identified using the inclusion criteria. The prevalence of Angle class III malocclusion ranged from 0 to 26.7% in different populations reported in the literature examined. Meta-regression analysis showed no statistically significant association between prevalence rates and the method of assessment, age group and year of the study. However, much of the study-to-study variation (approximately 40%) could be explained by population. Conclusion: These results suggest that the prevalence of Angle class III malocclusion varies greatly within different races and geographic regions. Chinese and Malaysian populations have a higher prevalence of Angle class III malocclusion compared to other racial groups, while Indian populations have a lower prevalence than all other racial groups examined. 展开更多
关键词 malocclusion class III PREVALENCE Systematic REVIEW META-ANALYSIS
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Successful Treatment of Postpeak Stage Patients with ClassⅡ Division 1 Malocclusion Using Non-extraction and Multiloop Edgewise Archwire Therapy: A Report on 16 Cases 被引量:1
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作者 Jun Liu Ling Zou +4 位作者 Zhi-he Zhao Neala Welburn Pu Yang Tian Tang Yu Li 《International Journal of Oral Science》 SCIE CAS CSCD 2009年第4期207-216,共10页
Aim To determine cephalometrically the mechanism of the treatment effects of non-extraction and multiloop edgewise archwire (MEAW) technique on postpeak Class Ⅱ Division 1 patients. Methodology In this retrospectiv... Aim To determine cephalometrically the mechanism of the treatment effects of non-extraction and multiloop edgewise archwire (MEAW) technique on postpeak Class Ⅱ Division 1 patients. Methodology In this retrospective study, 16 postpeak Class Ⅱ Division 1 patients successfully corrected using a non-extraction and MEAW technique were cephalometrically evaluated and compared with 16 matched control subjects treated using an extraction technique. Using CorelDRAW software, standardized digital cephalograms preand post-active treatments were traced and a reference grid was set up. The superimpositions were based on the cranial base, the mandibular and the maxilla regions,and skeletal and dental changes were measured. Changes following treatment were evaluated using the paired-sample t-test. Student's t-test for unpaired samples was used to assess the differences in changes between the MEAW and the extraction control groups. Results The correction of the molar relationships comprised 54% skeletal change (mainly the advancement of the mandible) and 46% dental change. Correction of the anterior teeth relationships comprised 30% skeletal change and 70% dental change. Conclusion The MEAW technique can produce the desired vertical and sagittal movement of the tooth segment and then effectively stimulate mandibular advancement by utilizing the residual growth potential of the condyle. 展开更多
关键词 CEPHALOMETRY class Division 1 malocclusion mandibular advancement multiloop edgewise archwire(MEAW) non-extraction postpeak stage
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Pressure from the lips and the tongue in children with class III malocclusion 被引量:6
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作者 RUAN Wen-hua SU Ji-mei YE Xiao-wei 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第5期296-301,共6页
Objective:To discuss possible relationships between class III malocclusion and perioral forces by measuring the pressure from the lips and the tongue of children with class III malocclusion. Methods:Thirty-one childre... Objective:To discuss possible relationships between class III malocclusion and perioral forces by measuring the pressure from the lips and the tongue of children with class III malocclusion. Methods:Thirty-one children with class III malocclusion were investigated and their perioral forces were measured at rest and during swallowing under natural head position by a custom-made miniperioral force computer measuring system. Results:The resting pressures exerted on the labial side and palatine side of the upper left incisor, as well as the labial side and lingual side of the lower left incisor, were 0 g/cm2, 0 g/cm2, 0.57 g/cm2 and 0.23 g/cm2, respectively. Correspondingly, the swallowing forces were 2.87 g/cm2, 5.97 g/cm2, 4.09 g/cm2 and 7.89 g/cm2, respectively. No statistical difference between muscular pressure and gender existed. During swallowing, the lingual forces were significantly higher than the labial forces (P<0.01), however, at rest there was no significantly different force between these two sides. Compared to the normal occlusion patients, children with class III malocclusion had lower perioral forces. The upper labial resting forces (P<0.01), the lower labial resting forces (P<0.05) and all the swallowing pressures from the lips and the tongue (P<0.01) showed statistical differences between the two different occlusion conditions. Meanwhile, no significant difference was found for the resting pressure from the tongue between class III malocclusion and normal occlusion. Conclusion:Patients with class III malocclusion have lower perioral forces and this muscle hypofunction may be secondary to the spatial relations of the jaws. The findings support the spatial matrix hypothesis. 展开更多
关键词 Ⅲ类咬合不正 儿童 嘴唇 舌头 压力测量
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Distalization of maxillary molars for Class Ⅱ malocclusion with Chinese Ni-Ti coil-springs appliance 被引量:1
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作者 段银钟 武俊杰 +1 位作者 陈学鹏 钱红 《Journal of Medical Colleges of PLA(China)》 CAS 2005年第6期373-376,共4页
Objective: To evaluate the effects of the Chinese Ni-Ti coil-springs appliance on distalization of maxillary molars and the reciprocal effects on the anchorage teeth. Methods:Twenty-four adolescent patients(12 boys,12... Objective: To evaluate the effects of the Chinese Ni-Ti coil-springs appliance on distalization of maxillary molars and the reciprocal effects on the anchorage teeth. Methods:Twenty-four adolescent patients(12 boys,12 girls) with Class Ⅱ malocclusion were selected and the coil-springs appliance was used during the treatment. Pre- and postdistalization lateral cephalometric radiographs were analyzed and compared. Results: The average time for the correction of Class Ⅱ molar relationship was 4.6 months. And the mean distance of molar distalization was 4.4 mm. The Chinese Ni-Ti coil-springs also demostrated less tipping and better bodily movement of maxillary molars. Conclusion: This study suggests that the Chinese Ni-Ti coil-springs appliance distalizes the upper molar significantly, while there is no remarkable loss of anchorage because of patients’ wearing headgear with J hooks at night and Class Ⅱ elastics in the day time. 展开更多
关键词 上颌骨磨牙 咬合不正 治疗 口腔材料
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Orthopedic Correction of Class III Malocclusions during Mixed Dentition
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作者 Hussein N. Al-Khalifa 《Open Journal of Stomatology》 2014年第7期372-380,共9页
Objective: The aim of this study was to compare the effect of face mask (FM) and Maxillary Bite Block (BB) in skeletal class III patients during mixed dentition with control group of the same class during their growth... Objective: The aim of this study was to compare the effect of face mask (FM) and Maxillary Bite Block (BB) in skeletal class III patients during mixed dentition with control group of the same class during their growth. Materials & Methods: Forty-two patients were selected based on clinical and cephalometric examination, with age ranged from 6 to 8 years according to definite criteria. Patients were classified into two groups: treated and control groups;all records were taken for every patient. Lateral cephalometric films were traced before and after treatment and analyzed. Results: Intermaxillary skeletal variables showed significant improvement in the treated group with an average increase of ANB angle (Maxilo mandibular difference angle) of 3.14&deg;and an average increase in wits appraisal 2.15 mm. Conclusion: The study concluded that, treatment with face mask and maxillary bite block induced significant dentoskeletal changes. 展开更多
关键词 ORTHOPEDIC CORRECTION class III malocclusions MIXED DENTITION
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Nonsurgical orthodontic treatment for an adult with skeletal open bite, class III malocclusion and posterior crossbite: A case report
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作者 Yongming Li Jiaqiang Liu Yin Ding 《Open Journal of Stomatology》 2013年第3期241-245,共5页
Class III malocclusion associated with skeletal anterior open bite pattern in adults can be a challenging orthodontic problem, especially for the nonsurgical treatment. Conventionally, several treatment alternatives a... Class III malocclusion associated with skeletal anterior open bite pattern in adults can be a challenging orthodontic problem, especially for the nonsurgical treatment. Conventionally, several treatment alternatives are available such as tooth extraction, molar intrusion, and absolute anchorage system or orthognathic surgical correction. Although correction with surgery may be the most effective and stable way, many patients refused surgical treatment plan because of the costs and traumas it may bring. We reported a nonsurgical orthopedic treatment of 22-year-old male with severe skeletal anterior open bite, dental Class III malocclusion, posterior crossbite and a high mandibular plane angle. The patient refused surgery and extraction. So we formulated a treatment plan consisting of using rapid palatal expansion appliance to expand the maxilla, standard edgewise brackets to align the teeth, Class III elastics to correct the canines, premolars, and molars relationship, reverse curve of the nickel-titanium wire combined with anterior vertical elastics to intrude molars and correct open bite. In this case, without suffering of surgery, the posterior crossbite was ideally corrected, and ideal overjet and overbite relationships and functional occlusion were all achieved. The patient obtained satisfactory occlusal as well as functional and stable results. 展开更多
关键词 class III malocclusion Open BITE NONSURGICAL Orthopedic TREATMENT ORTHODONTIC TREATMENT
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广东地区安氏Ⅰ类双牙弓前突错(牙合)畸形拔牙正畸后女性唇部软组织厚度与唇部回收的关系
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作者 曹芳 王翠芳 王一珏 《中国医疗美容》 2024年第1期55-58,共4页
目的探究广东地区安氏Ⅰ类双牙弓前突错(牙合)畸形拔牙正畸后女性唇部软组织厚度及唇部回收的关系。方法选取2020年1月~2023年12月期间在我院门诊正畸科就诊的成年女性患者120例,所有患者接受头颅侧位片拍摄并测量唇部软组织厚度,依据... 目的探究广东地区安氏Ⅰ类双牙弓前突错(牙合)畸形拔牙正畸后女性唇部软组织厚度及唇部回收的关系。方法选取2020年1月~2023年12月期间在我院门诊正畸科就诊的成年女性患者120例,所有患者接受头颅侧位片拍摄并测量唇部软组织厚度,依据唇厚度分类标准分为薄唇型、均衡型及厚唇型,分析不同唇型软组织厚度切牙上唇回收比及唇厚度与切牙上唇相关性。结果三组不同唇厚度组在对比△Is-Y、△Ls-Y指标上均存在差异,P<0.05,其中薄唇组唇部回收量大,唇厚组回收量较小;正畸前及正畸治疗后,唇厚组患者软组织厚度高于薄唇组、均衡组,在正畸治疗前后三组软组织厚度均呈现改变,但薄唇组改变情况更为显著,P<0.05;唇部软组织厚度与唇部回收程度呈负相关,当回收程度越高则表明唇部软组织厚度越薄,P<0.05。结论女性错(牙合)人群中在正畸治疗期间上唇软组织厚度会影响到唇部回收程度,且呈负相关,为此需采取针对性措施调整正畸治疗方案,保障正畸效果。 展开更多
关键词 广东地区 安氏类双牙弓 前突错(牙合) 正畸 唇部软组织厚度 唇部回收
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Ⅰ类切口围术期抗菌药物预防使用合理性分析
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作者 崔晓飞 《临床研究》 2024年第7期48-51,共4页
目的分析濮阳油田总医院Ⅰ类切口围术期抗菌药物预防使用合理性情况,为后续临床抗菌药物的管理提供参考性依据。方法将濮阳油田总医院2023年1月至2023年6月开展的635例行Ⅰ类切口手术患者作为本次研究对象,回顾性分析接受Ⅰ类切口手术... 目的分析濮阳油田总医院Ⅰ类切口围术期抗菌药物预防使用合理性情况,为后续临床抗菌药物的管理提供参考性依据。方法将濮阳油田总医院2023年1月至2023年6月开展的635例行Ⅰ类切口手术患者作为本次研究对象,回顾性分析接受Ⅰ类切口手术患者在围术期接受抗菌药物的治疗效果。结果本次研究中收入的635例Ⅰ类切口手术患者中,共有214例患者使用抗菌药物,针对每个科室Ⅰ类切口患者围术期抗菌药物预防性使用情况进行分析,脊柱外科患者使用抗菌药物占66.04%,神经外科占64.00%,手足与创伤骨科占50.90%;甲状腺血管外科、乳腺外科、疝与腹壁外科与泌尿外科中未使用任何抗菌药物,差异有统计学意义(P<0.05)。Ⅰ类切口围术期使用抗菌药物的种类主要为第一、二代头孢菌素类,以静脉给药为主。共使用抗菌药物7种,其中头孢唑林占59.35%,头孢呋辛占26.17%,差异有统计学意义(P<0.05);抗菌药物合理使用率为86.45%,不合理使用率为13.55%,主要以无指征预防用药、超疗程用药为主,差异有统计学意义(P<0.05)。结论本院接受Ⅰ类切口手术的患者中在围术期采取预防使用抗菌药物情况大部分合理,但仍存在少部分不合理情况,故应加强干预,保证抗菌药物的合理使用,提高抗菌药物合理率,保证患者用药安全。 展开更多
关键词 类切口 围术期 抗菌药物 合理性
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医嘱点评干预对医院Ⅰ类切口手术患者围术期抗菌药物使用的影响
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作者 吴玲英 王会燕 《抗感染药学》 2024年第5期483-486,共4页
目的:分析医嘱点评干预对医院Ⅰ类切口手术患者围术期抗菌药物使用的影响,为临床Ⅰ类切口手术患者围术期抗菌药物的合理使用提供参考。方法:选取2020年1月—2021年6月(点评干预前,164例)和2021年7月—2022年12月(点评干预后,164例)抚州... 目的:分析医嘱点评干预对医院Ⅰ类切口手术患者围术期抗菌药物使用的影响,为临床Ⅰ类切口手术患者围术期抗菌药物的合理使用提供参考。方法:选取2020年1月—2021年6月(点评干预前,164例)和2021年7月—2022年12月(点评干预后,164例)抚州市东乡区人民医院收治的328例Ⅰ类切口手术患者作为研究对象,比较和分析点评干预前后不同手术患者抗菌药物的预防使用率和不合理用药类型。结果:医嘱点评干预后Ⅰ类切口手术患者抗菌药物的预防使用率明显低于医嘱点评干预前(28.66%vs 79.27%,P<0.05),并且在腹股沟疝手术、乳腺手术、骨科手术、白内障手术、甲状腺疾病手术等术式上的抗菌药物预防使用率均低于医嘱点评干预前(P<0.05);而医嘱点评结果显示,医嘱点评干预后患者围术期抗菌药物在给药时机、药品选用、用药疗程、用法用量、联合用药等方面的不合理率均明显低于医嘱点评干预前(P<0.05)。结论:医嘱点评干预确实可以有效改善医院Ⅰ类切口手术患者围术期抗菌药物的不合理使用情况,从而提升临床的合理用药水平,进而保障患者的用药安全。 展开更多
关键词 类切口手术 围术期 医嘱点评 抗菌药物
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五龙鹅MHC ClassⅠ基因克隆及同源建模研究 被引量:6
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作者 贾晓晖 王宝维 +7 位作者 王雷 李桢 张名爱 吴晓平 刘光磊 杨志刚 龙芳羽 张旭晖 《遗传》 CAS CSCD 北大核心 2006年第9期1087-1092,共6页
主要组织相容性复合体(MHC)与动物机体对外源性抗原的免疫应答之间存在关联。从GenBank/DDBJ/EMBL基因库中读取鸡、其他鸟类、爬行类和哺乳类的MHC ClassⅠ基因进行序列分析设计引物,使用LA—PCR法从五龙鹅的基因组中克隆了MHC Clas... 主要组织相容性复合体(MHC)与动物机体对外源性抗原的免疫应答之间存在关联。从GenBank/DDBJ/EMBL基因库中读取鸡、其他鸟类、爬行类和哺乳类的MHC ClassⅠ基因进行序列分析设计引物,使用LA—PCR法从五龙鹅的基因组中克隆了MHC ClassⅠ基因序列(DNA序列和mRNA序列GenBank登录号分别为:AM114925和AM114924),并分析其基因组结构。运用生物信息学技术对测序结果进行分析显示:基因组DNA由8个外显子和7个内含子组成,与鸡基因序列同源率为60.8%~64.1%,与人的同源率为42.9%。分子进化树进一步揭示了五龙鹅与鸡、其他鸟类、爬行类、哺乳类以及人类的进化关系,同源建模分析发现该基因由氨基末端结构域和羧基末端结构域构成。 展开更多
关键词 MHC class 五龙鹅 基因组 分子进化树 氨基酸同源率 同源建模
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ClassⅠ新城疫病毒NP基因分子特征的研究 被引量:3
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作者 刘晓文 吴双 +3 位作者 胡顺林 王晓泉 刘慧谋 刘秀梵 《中国家禽》 北大核心 2009年第23期11-14,共4页
为分析2002~2007年Class Ⅰ新城疫病毒分离株NP基因的分子特征,以及其在新城疫病毒演化过程中所起的作用,采用RT-PCR方法扩增分离株的核衣壳蛋白(NP)基因,并进行了测序,应用生物信息学软件分析NDVNP基因的分子特征。根据NP基因序列的... 为分析2002~2007年Class Ⅰ新城疫病毒分离株NP基因的分子特征,以及其在新城疫病毒演化过程中所起的作用,采用RT-PCR方法扩增分离株的核衣壳蛋白(NP)基因,并进行了测序,应用生物信息学软件分析NDVNP基因的分子特征。根据NP基因序列的遗传发生分析表明,ClassⅠ NDV2型毒株与DE/R49-99和美国株亲缘关系较近,而3型毒株与其他ClassⅠ毒株遗传关系较远,具有地区特色,形成一个独立的分支。生物信息学软件分析表明,NP蛋白N端1~400氨基酸比较保守,而C端序列(特别是氨基酸残基400~480)高度变异。ClassⅠ病毒2型毒株与国外分离株亲缘关系较近,可能有共同的起源;而3型毒株独立于其他ClassⅠ毒株,具有独特性,这两种毒株的来源不同;不同基因型NP蛋白之间比较保守,但是P蛋白的C端发生了多个氨基酸的变异,对其功能的影响还需进一步探讨。 展开更多
关键词 新城疫病毒 classclass 核蛋白基因 发生分析
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马铃薯class Ⅰ patatin基因在试管块茎形成中的功能 被引量:2
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作者 司怀军 柳俊 谢从华 《作物学报》 CAS CSCD 北大核心 2006年第9期1406-1409,共4页
将正反义classⅠpatatin基因导入马铃薯品种甘农薯2号中,有2个转正义基因株系试管块茎的可溶性蛋白含量和LAH活性与对照相比有不同程度的增加,有3个转反义基因株系的可溶性蛋白质含量下降,并且所有转反义基因植株的LAH活性都不同程度地... 将正反义classⅠpatatin基因导入马铃薯品种甘农薯2号中,有2个转正义基因株系试管块茎的可溶性蛋白含量和LAH活性与对照相比有不同程度的增加,有3个转反义基因株系的可溶性蛋白质含量下降,并且所有转反义基因植株的LAH活性都不同程度地降低。试管块茎的诱导结果表明,有1个转正义基因株系的结薯株率和单株结薯数比其对照明显增加,有2个转反义基因株系的结薯株率和单株结薯数比对照明显减少,说明该classⅠpatatin基因参与了马铃薯试管块茎的形成及其调控。 展开更多
关键词 马铃薯 class patatin基因 转化 块茎形成
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新城疫病毒ClassⅠ强毒株HN单克隆抗体的研制及免疫学反应 被引量:5
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作者 孙英杰 陈鸿军 +4 位作者 宋翠萍 于洋 仇旭升 于圣青 丁铲 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2010年第5期464-466,共3页
目的:制备抗新城疫病毒(NDV)ClassⅠ强毒分离株9a5b的单克隆抗体。方法:以9a5b尿囊液为免疫原,接种6周龄BALB/c小鼠,以血凝抑制(HI)和间接免疫荧光(IFA)检测所获得的mAb。结果:成功制备获得NDV血凝素(HN)特异性mAb4株。免疫特性鉴定表明... 目的:制备抗新城疫病毒(NDV)ClassⅠ强毒分离株9a5b的单克隆抗体。方法:以9a5b尿囊液为免疫原,接种6周龄BALB/c小鼠,以血凝抑制(HI)和间接免疫荧光(IFA)检测所获得的mAb。结果:成功制备获得NDV血凝素(HN)特异性mAb4株。免疫特性鉴定表明:在这4株mAb中,3H7和3H9株仅具有IFA和HI效价,且呈现ClassI毒株特异性。结论:该抗体材料为NDVClassⅠ和Ⅱ毒株的血清学鉴定提供极大的便利工具,并为NDVHN功能及受体学研究奠定了基础。 展开更多
关键词 新城疫病毒 class 强毒株 HN 单克隆抗体
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ClassⅠ新城疫病毒概述 被引量:8
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作者 金仕强 孟春春 +3 位作者 仇旭升 于圣青 丁铲 左之才 《动物医学进展》 CSCD 北大核心 2012年第4期102-106,共5页
新城疫病毒是影响养禽业健康发展的主要病原之一。新城疫病毒只有一个血清型,但根据亲源关系可以划分为ClassⅠ和ClassⅡ两大类。ClassⅠ新城疫病毒于2006年被证实存在,当前可被细分为9个基因型(1~9)。ClassⅠ所有自然分离株均为无毒... 新城疫病毒是影响养禽业健康发展的主要病原之一。新城疫病毒只有一个血清型,但根据亲源关系可以划分为ClassⅠ和ClassⅡ两大类。ClassⅠ新城疫病毒于2006年被证实存在,当前可被细分为9个基因型(1~9)。ClassⅠ所有自然分离株均为无毒株或弱毒株,但有经人工传代返强的报道,由于其分布广泛,数量庞大,对养禽业有巨大的潜在威胁。为了更好的了解ClassⅠ新城疫毒株的生物学特性,为研究ClassⅠ新城疫病毒提供参考,现将其基因组结构、分子流行病学及检测方法等综述如下。 展开更多
关键词 class新城疫病毒 基因型 分子流行病学
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新城疫病毒ClassⅠ与ClassⅡ毒株交叉血凝抑制试验快速分类 被引量:1
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作者 王晓泉 刘晓文 +3 位作者 胡顺林 刘慧谋 刘文博 刘秀梵 《中国家禽》 北大核心 2011年第4期14-17,21,共5页
ClassⅠ新城疫病毒(NDV)分离自健康家禽泄殖腔棉拭子,其基因序列与ClassⅡNDV存在很大差异,为了建立简单快速的NDV分类方法,用SPF鸡制备ClassⅠ和ClassⅡ毒株多价抗血清分别与选取的ClassⅠ和ClassⅡ代表毒株进行交叉血凝抑制反应。结... ClassⅠ新城疫病毒(NDV)分离自健康家禽泄殖腔棉拭子,其基因序列与ClassⅡNDV存在很大差异,为了建立简单快速的NDV分类方法,用SPF鸡制备ClassⅠ和ClassⅡ毒株多价抗血清分别与选取的ClassⅠ和ClassⅡ代表毒株进行交叉血凝抑制反应。结果表明所有ClassⅠ毒株比同ClassⅡ毒株的交叉血凝抑制值高8~64(3~6log2)倍;而ClassⅡ毒株与ClassⅡ抗血清的HI效价比同ClassⅠ毒株的交叉血凝抑制值高2~8(1~3log2)倍,结果与基因分型高度一致,说明可以用交叉血凝抑制试验进行初步分类。本研究结果显示,NDVClassⅠ弱毒株血凝性与广泛应用的NDV弱毒疫苗株不同,基因序列分析可以部分解释这种差异,另外可能HN蛋白的空间结构也会影响其与血凝素结合的特性。 展开更多
关键词 新城疫病毒 class class 交叉血凝抑制性
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草鱼MHC class Ⅰ基因多态性及其与鱼体抗柱形病能力关系分析 被引量:5
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作者 杨玲 孟庆磊 +5 位作者 张龙岗 张志山 安丽 董学飒 刘羽清 付佩胜 《长江大学学报(自科版)(中旬)》 CAS 2013年第6期42-47,50,共7页
用1对引物HMC6-S和MHC6-A分别从草鱼(Ctenopharyngodon idellus)40尾抗病个体和40尾易染个体的基因组DNA中扩增MHC基因片段,扩增产物长度为262bp。在262bp的核苷酸序列中,有50个(19%)多态位点。在其编码的87个氨基酸位点中,有16个多态位... 用1对引物HMC6-S和MHC6-A分别从草鱼(Ctenopharyngodon idellus)40尾抗病个体和40尾易染个体的基因组DNA中扩增MHC基因片段,扩增产物长度为262bp。在262bp的核苷酸序列中,有50个(19%)多态位点。在其编码的87个氨基酸位点中,有16个多态位点(18.39%),其中有10个位点发生在多肽结合位点上(62.5%)。对核苷酸替代的类型和位点进行分析,发现多肽结合位点的非同义碱基替代率与同义碱基替代率均大于非多肽结合位点的非同义碱基替代率与同义碱基替代率,表明氨基酸替代替换集中出现在多肽结合位点(PBR)上。分析80个个体的测序结果,发现有17种不同的MHC classⅠ等位基因,编码17种不同的氨基酸序列。等位基因A、B、C、D是2个群体共有的,其中等位基因A出现的频率最高,占总样本数量的36.25%;等位基因E、F、G、H、I、P只出现在抗病个体中,等位基因J、K、L、M、N、O、Q只出现在易染个体中。 展开更多
关键词 主要组织相容性复合体(MHC) MHC class 多态性 抗病力 草鱼(Cteno pharyngodon idellus)
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新城疫病毒ClassⅠ强毒株磷蛋白在细胞中的表达与定位 被引量:2
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作者 程靖华 孙英杰 +5 位作者 陈鸿军 仇旭升 宋翠萍 于圣青 吴艳涛 丁铲 《中国动物传染病学报》 CAS 2013年第1期17-22,共6页
根据ClassI新城疫病毒分离株9a5b编码序列设计特异性引物扩增P蛋白基因,原核表达后利用纯化的蛋白免疫BALB/C小鼠制备单克隆抗体,经二次亚克隆后筛选到4株针对P蛋白的单克隆抗体,利用所得单抗进行P蛋白在细胞内定位分析,NDV9a5b株... 根据ClassI新城疫病毒分离株9a5b编码序列设计特异性引物扩增P蛋白基因,原核表达后利用纯化的蛋白免疫BALB/C小鼠制备单克隆抗体,经二次亚克隆后筛选到4株针对P蛋白的单克隆抗体,利用所得单抗进行P蛋白在细胞内定位分析,NDV9a5b株按5M.O.I感染量接种DFl单层细胞,当PI=4h时,P蛋白呈点状弥散分布于细胞浆内;PI=6~8h时,P蛋白聚集于细胞核周围;PI=12h时,P蛋白呈单极化聚集于细胞核一侧;而PI=16h后开始形成合胞体,P蛋白在单个细胞中仍呈现单极分布;当PI=48h时,细胞核已发生碎裂,荧光强度有所减弱。本研究为深入开展P蛋白在病毒增殖以及细胞周期中的作用机制研究奠定了基础。 展开更多
关键词 新城疫病毒 class P蛋白 细胞内定位
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ClassⅠ新城疫病毒核衣壳蛋白在体外细胞感染过程中表达定位的检测 被引量:1
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作者 孙英杰 陈鸿军 +4 位作者 詹媛 仇旭升 于洋 于圣青 丁铲 《中国预防兽医学报》 CAS CSCD 北大核心 2011年第2期85-88,共4页
为分析核衣壳蛋白(NP)在新城疫病毒(NDV)感染真核细胞过程中细胞内定位的情况,本研究以NDV Class I强毒株9a5b作为免疫原制备4株NP特异性单克隆抗体(MAb),并将病毒株9a5b按5 MOI感染量接种DF1单层细胞,当接种后1 h~3 h时,NP有极少量表... 为分析核衣壳蛋白(NP)在新城疫病毒(NDV)感染真核细胞过程中细胞内定位的情况,本研究以NDV Class I强毒株9a5b作为免疫原制备4株NP特异性单克隆抗体(MAb),并将病毒株9a5b按5 MOI感染量接种DF1单层细胞,当接种后1 h~3 h时,NP有极少量表达并逐量增加,主要呈点状分布于细胞浆内;当接种后4 h~20 h时,NP聚集于细胞浆中,聚集数量与感染时间成正比;感染24 h后,细胞核碎裂,NP散在分布于细胞浆中。表明NP作为立早期蛋白,始终在胞浆中进行复制;在病毒感染初期,NP介导核糖核蛋白复合物发挥转录和翻译功能。本研究为深入开展NP与病毒mRNA转录、复制和包装的相互关系机制的研究奠定了基础。 展开更多
关键词 新城疫病毒 class 核蛋白 细胞内定位
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建鲤MHC classⅠ基因全长cDNA的克隆与序列分析 被引量:1
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作者 周凯 张成锋 +2 位作者 王建新 李冰 朱健 《淡水渔业》 CSCD 北大核心 2011年第1期29-35,共7页
采用同源克隆和末端快速扩增(RACE)方法克隆了建鲤(Cyprinus carpio var.jian)MHC classⅠ基因全长cD-NA并进行了序列分析。结果显示:实验得到了1914 bp的建鲤的MHC classⅠ全长cDNA序列;建鲤MHC classⅠ基因包括1044 bp的开放阅读框(OR... 采用同源克隆和末端快速扩增(RACE)方法克隆了建鲤(Cyprinus carpio var.jian)MHC classⅠ基因全长cD-NA并进行了序列分析。结果显示:实验得到了1914 bp的建鲤的MHC classⅠ全长cDNA序列;建鲤MHC classⅠ基因包括1044 bp的开放阅读框(ORF),118 bp的5'非编码区(UTR)以及752 bp的3'非编码区(UTR),含有保守的半胱氨酸残基,N-糖基化位点。氨基酸序列比对结果显示,建鲤MHC classⅠ与日本鲤的MHC classⅠ相似性最高,为66.0%;与虹鳟、大西洋鲑、青鳉、红鳍东方鲀的相似性分别为54.5%、57.9%、44.3%、42.0%,与小鼠、大鼠、人的相似性分别为29.1%、28.7%、29.7%。 展开更多
关键词 建鲤(Cyprinus carpio var.jian) MHC class 克隆 序列分析
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