乳腺癌组织中紧密连接蛋白CLDN6、基质金属蛋白酶2和Snail的表达及临床意义

目的分析乳腺癌组织及癌旁正常乳腺组织Claudin6、MMP2及Snail的蛋白水平,三者在乳腺癌组织中表达的相关性,上述三种蛋白表达与患者病理分期、淋巴结转移、肿瘤大小和年龄的相关性。方法选取2021年8月—2022年7月本院病理中心的38例乳腺癌和24例癌旁正常乳腺组织标本,采用免疫组化Sp法进行实验研究,检测Claudin6、MMP2及Snail的表达情况;分析乳腺癌组织上述三种蛋白表达及其与临床病理特征的相关性。结果癌组织中Claudin6阳性率较正常组织低,差异显著(P<0.01);癌组织中MMP2和Snail阳性表达率较正常组织高,差异具有统计学意义(P<0.05)。癌组织中Claudin6与MMP2表达呈负相关(P<0.01),Claudin6与Snai表达呈负相关(P<0.001),MMP2与Snail表达呈正相关(P≤0.001)。Claudin6、MMP2和Snail的表达水平与病理分期、肿瘤大小和患者年龄均不相关(P>0.05);淋巴结转移病例Claudin6阳性率显著低于无淋巴结转移病例(P<0.01);相反,淋巴结转移病例MMP2和Snail阳性率明显高于无淋巴结转移病例(P<0.05)。结论Claudin6蛋白在乳腺癌组织中低表达、MMp2及Snail蛋白在乳腺癌组织中高表达,在乳腺癌组织中Claudin6与MMP2蛋白、Claudin6与Snail蛋白表达均呈负相关;MMP2和Snail蛋白表达呈正相关。淋巴结转移病例CLDN6阳性率显著低于无淋巴结转移病例;淋巴结转移病例MMP2和Snail阳性率高于无淋巴结转移病例;推测紧密连接蛋白6可能通过阻断Snail/MMP2信号轴,抑制乳腺癌的上皮间质转化和转移。

CLDN6基因对脑膜瘤组织中细胞增殖、侵袭相关基因表达的影响

目的:研究Claudin6(CLDN6)基因对脑膜瘤组织中细胞增殖、侵袭相关基因表达的影响。方法:选择在宜宾市第一人民医院接受手术治疗的脑膜瘤患者以及脑外伤患者,收集脑膜瘤组织和正常脑膜组织,采用免疫组化试剂盒测定CLDN6的蛋白阳性率,采用酶联免疫吸附试剂盒测定CLDN6、增殖基因、侵袭基因的蛋白表达量。结果:脑膜瘤组织中CLDN6的蛋白含量及蛋白阳性率均显著低于正常脑膜组织;脑膜瘤组织中PHH3、MCM2、E2F3、MMP2、MMP9、N-cadherin、Vimentin的蛋白含量显著高于正常脑膜组织,BAG-1、Caspase-3的蛋白含量显著低于正常脑膜组织;CLDN6阳性脑膜瘤组织中PHH3、MCM2、E2F3、MMP2、MMP9、N-cadherin、Vimentin的蛋白含量显著低于CLDN6阴性脑膜瘤组织,BAG-1、Caspase-3的蛋白含量显著高于CLDN6阴性脑膜瘤组织。结论:脑膜瘤组织中CLDN6基因低表达与细胞异常增殖、侵袭密切相关。

脑膜瘤组织中CLDN6基因表达量与MMPs/TIMPs、EMT基因表达量的相关性研究

目的:研究脑膜瘤组织中Claudins6(CLDN6)基因表达量与基质金属蛋白酶(MMPs)/基质金属蛋白酶组织抑制因子(TIMPs)、上皮间质转化(EMT)基因表达量的相关性。方法:选择在本院手术切除的脑膜瘤组织样本,同期在宜宾市第一人民医院开颅减压术中收集的正常蛛网膜组织,检测组织中CLDN6、MMPs/TIMPs、EMT基因的表达量。结果:脑膜瘤组织中CLDN6的蛋白表达量显著低于正常蛛网膜组织;脑膜瘤组织中EMMPRIN、MMP2、MMP9、Vimentin、N-cadherin的蛋白表达量均显著高于正常蛛网膜组织,TIMP1、TIMP2、E-cadherin、α-catenin的蛋白表达量均显著低于正常蛛网膜组织;CLDN6高表达脑膜瘤组织中EMMPRIN、MMP2、MMP9、Vimentin、N-cadherin的蛋白表达量显著低于CLDN6低表达脑膜瘤组织,TIMP1、TIMP2、E-cadherin、α-catenin的蛋白表达量显著高于CLDN6低表达脑膜瘤组织。结论:脑膜瘤组织中CLDN6基因的低表达能够引起MMPs水解活性增强、诱导上皮间质转化,进而促进脑膜瘤的浸润性生长。

Effect of CLDN6 gene on cell proliferation and invasion-related gene expression in meningioma tissue

Objective:To study the effect of Claudin6 (CLDN6) gene on cell proliferation and invasion-related gene expression in meningioma tissue.Methods:Patients with meningioma and patients with brain trauma who received surgical treatment in Yibin First People's Hospital between May 2014 and March 2017 were selected, the meningioma and normal meninges tissue were collected, the positive protein rate of CLDN6 was determined by immunohistochemical kit, and the protein expression of CLDN6, invasion genes and proliferation genes were determined by the enzyme-linked immunosorbent assay kit.Results:The protein content and positive protein rate of CLDN6 in meningioma tissue were significantly lower than those in normal meninges tissue;PHH3, MCM2, E2F3, MMP2, MMP9, N-cadherin and Vimentin protein contents in meningioma tissue were significantly higher than those in normal meninges tissue while BAG-1 and Caspase-3 protein contents were significantly lower than those in normal meninges tissue;PHH3, MCM2, E2F3, MMP2, MMP9, N-cadherin and Vimentin protein contents in CLDN6-positive meningioma tissue were significantly lower than those in CLDN6-negative meningioma tissue while BAG-1 and Caspase-3 protein contents were significantly higher than those in CLDN6-negative meningioma tissue.Conclusions:The low expression of CLDN6 gene in meningioma tissue is closely related to the abnormal proliferation and invasion of cells.

Correlation of Claudins6 (CLDN6) gene expression in meningioma tissue with the expression of matrix metalloproteinases (MMPs)/tissue inhibitors of matrix metalloproteinase (TIMPs) and epithelial-mesenchymal transition (EMT) genes

Objective:To study the correlation of Claudins6 (CLDN6) gene expression in meningioma tissue with the expression of matrix metalloproteinases (MMPs)/tissue inhibitors of matrix metalloproteinase (TIMPs) and epithelial-mesenchymal transition (EMT) genes.Methods:Meningioma tissue samples that were surgically removed in Yibin First People's Hospital between April 2014 and May 2017 were selected, normal arachnoid tissue samples that were collected from decompressive craniectomy in Yibin First People's Hospital during the same period were selected, and the expression of CLDN6, MMPs/TIMPs and EMT genes in tissues were determined.Results: CLDN6 protein expression in meningioma tissue was significantly lower than that in normal arachnoid tissue;EMMPRIN, MMP2, MMP9, Vimentin and N-cadherin protein expression in meningioma tissue were significantly higher than those in normal arachnoid tissue while TIMP1, TIMP2, E-cadherin andα-catenin protein expression were significantly lower than those in normal arachnoid tissue;EMMPRIN, MMP2, MMP9, Vimentin and N-cadherin protein expression in meningioma tissue with higher CLDN6 expression were significantly lower than those in meningioma tissue with lower CLDN6 expression while TIMP1, TIMP2, E-cadherin andα-catenin protein expression were significantly higher than those in meningioma tissue with lower CLDN6 expression. Conclusion: Lowly expressed CLDN6 gene in meningioma tissue can increase the hydrolysis activity of MMPs, induce epithelial-mesenchymal transition and thus promote the invasive growth of meningioma.