Advances in clinical applications of bioceramics in the new regenerative medicine era

In this editorial,we comment on the hard and soft tissue applications of different ceramic-based scaffolds prepared by different mechanisms such as 3D printing,sol-gel,and electrospinning.The new concept of regenerative medicine relies on biomaterials that can trigger in situ tissue regeneration and stem cell recruitment at the defect site.A large percentage of these biomaterials is ceramic-based as they provide the essential requirements of biomaterial principles such as tailored multisize porosity,antibacterial properties,and angiogenic properties.All these previously mentioned properties put bioceramics on top of the hierarchy of biomaterials utilized to stimulate tissue regeneration in soft and hard tissue wounds.Multiple clinical applications registered the use of these materials in triggering soft tissue regeneration in healthy and diabetic patients such as bioactive glass nanofibers.The results were promising and opened new frontiers for utilizing these materials on a larger scale.The same results were mentioned when using different forms and formulas of bioceramics in hard defect regeneration.Some bioceramics were used in combination with other polymers and biological scaffolds to improve their regenerative and mechanical properties.All this progress will enable a larger scale of patients to receive such services with ease and decrease the financial burden on the government.

Clinical application value of long non-coding RNAs signatures of genomic instability in predicting prognosis of hepatocellular carcinoma

Hepatocellular carcinoma(HCC)presents challenges due to its high recurrence and metastasis rates and poor prognosis.While current clinical diagnostic and prognostic indicators exist,their accuracy remains imperfect due to their biol-ogical complexity.Therefore,there is a quest to identify improved biomarkers for HCC diagnosis and prognosis.By combining long non-coding RNA(lncRNA)expression and somatic mutations,Duan et al identified five representative lncRNAs from 88 lncRNAs related to genomic instability(GI),forming a GI-derived lncRNA signature(LncSig).This signature outperforms previously re-ported LncSig and TP53 mutations in predicting HCC prognosis.In this editorial,we comprehensively evaluate the clinical application value of such prognostic evaluation model based on sequencing technology in terms of cost,time,and practicability.Additionally,we provide an overview of various prognostic models for HCC,aiding in a comprehensive understanding of research progress in pro-gnostic evaluation methods.

Current Status of Clinical Application and Basic Research on Draba nemerosa Hebecarpa Jujube Lung-Diarrhoea Soup

The clinical application and basic research of Draba nemerosa Hebecarpa jujube diarrhoea lung soup focus on respiratory diseases and circulatory diseases, and its clinical application is more compared with the basic research, and Draba nemerosa Hebecarpa jujube diarrhoea lung soup is mostly used in the form of “Draba nemerosa Hebecarpa Hebecarpa jujube diarrhoea lung soup with additional subtractions or other formulas combining Draba nemerosa Hebecarpa Hebecarpa Hebecarpa jujube diarrhoea lung soup” in the clinical application. This article will focus on the main mechanisms of Draba nemerosa Hebecarpa jujube diarrhoea lung soup and its main components in the treatment of respiratory and circulatory diseases, and will give a review of the basic research on Draba nemerosa Hebecarpa Hebecarpa jujube diarrhoea lung soup as follows.

Study on The Current Status and Progress of Clinical Application of Ciprofol

Ciprofol,as a new type of short-acting intravenous anesthetic drug,belongs to the category of gamma-aminobutyric acid(GABA)receptor agonists.Its unique chemical structure,through the introduction of the cyclopropyl group in the isopropyl side chain of propofol,constructs a new type of chiral molecule,which significantly enhances the spatial effect,and improves the affinity for GABA receptors.Its pharmacological properties are characterized by high potency,rapid onset of action,rapid recovery,low accumulation,and minimal adverse reactions.Therefore,it has a wide range of applications in various endoscopic diagnostic and therapeutic operations,ICU sedation,and general anesthesia.In this paper,the related knowledge of ciprofol and the development of clinical application research are comprehensively sorted out and synthesized,to provide a solid theoretical basis for the rational application of ciprofol in clinical practice.At the same time,the future research direction of ciprofol will also be prospected to provide valuable references for research in related fields.

The Application of Psychological Nursing in Pediatric Clinical Nursing

Objective:To study the application effect of psychological nursing in pediatric clinical nursing.Method:100 pediatric patients who underwent treatment in our hospital from January 2019 to December 2020 were selected as the research subjects.They were randomly divided into a control group and an observation group,with 50 cases in each group.The control group received routine nursing care,while the observation group received psychological care based on routine nursing care.The nursing compliance,incidence of adverse events,and parental satisfaction of the two groups of pediatric patients were compared and analyzed.Result:Through psychological nursing intervention,it was found that the observation group had better nursing compliance,incidence of adverse events,and parental satisfaction than the control group,P<0.05,the differences are statistically significant.Conclusion:The application of psychological nursing in pediatric clinical nursing can improve the compliance of pediatric care,reduce the incidence of adverse events,enhance parental satisfaction,and have clinical promotion and application value.

Clinical Application Study of Super-Mini Percutaneous Nephrolithotomy in the Treatment of Kidney Stones

Objective:To observe the clinical efficacy and complication rates of different stone fragmentation techniques in the treatment of kidney stones.Methods:This study selected 100 patients with urinary stones treated at the Third Division General Hospital from 2021 to November 2023 as subjects.The control group(n=50)received conventional percutaneous nephrolithotomy(PCNL)for stone fragmentation,while the research group(n=50)received super-mini percutaneous nephrolithotomy(SMP)treatment.Surgical parameters,stone clearance rates,recurrence rates,and complication rates were compared between the two groups.Results:After treatment,the surgical parameters in the research group were significantly better than those in the control group.The research group had a higher stone clearance rate and lower rates of stone recurrence and complications(P<0.05).Conclusion:Compared with conventional PCNL,SMP shows better clinical outcomes for patients with kidney stones.It improves surgical parameters,increases stone clearance rates,and reduces both stone recurrence and complication rates,making it a valuable technique for clinical reference.

Clinical Application and Prospect of New Radiotherapy Technology in Cancer Treatment

Objective: Carbon ion therapy, a new radiotherapy technology, has shown its remarkable efficacy and potential in cancer treatment, especially in the treatment of refractory tumors. Methods: This paper clarifies the physical basis, technological change, and clinical practice effect of carbon ion therapy, comprehensively discusses the future prospects, and evaluates the clinical application effect. Results: The technology has significantly improved the treatment effectiveness and received a positive response from patients. Conclusion: Carbon ion therapy technology has become a major innovation in the field of cancer treatment. It not only has a profound impact on many current cancer therapy methods but also indicates the application blueprint for a wider range of cancer types in the future, showing a new chapter of medical technology advancement.

HIV/AIDS合并肝损伤患者的临床特点

目的总结HIV/AIDS合并肝损伤患者的临床特点。方法选取53例HIV抗体阳性合并肝损伤患者作为研究对象,根据肝脏组织病理结果分为病毒性肝炎组(n=27)、肝细胞肝癌组(n=15)和肝脏弥漫大B细胞淋巴瘤组(n=11)。比较三组患者的临床症状和腹部CT影像学特征,以及肝脏组织病理学和免疫表型。结果三组在消瘦、发热、上腹痛、浅表淋巴结肿大、丙氨酸转氨酶升高、碱性磷酸酶升高、甲胎蛋白升高方面差异均有统计学意义;三组在多发结节、单发结节、病灶不均匀强化、病灶轻度强化、病灶重度强化方面差异均有统计学意义(均P<0.05)。病毒性肝炎组镜下见肝小叶结构破坏,肝细胞肝癌组镜下见凝固性坏死组织,弥漫大B细胞淋巴瘤组镜下见肝小叶结构广泛破坏和星空现象。结论不同类型HIV/AIDS合并肝损伤患者临床症状、实验室检查结果、腹部CT影像学特征有一定差异,但其较难区分肝细胞癌和弥漫大B细胞淋巴瘤,两者鉴别诊断需借助肝脏组织病理学和免疫表型方法。

Applications and safety of gold nanoparticles as therapeutic devices in clinical trials

Use of gold nanoparticles(GNPs)in medicine is an emerging field of translational research with vast clinical implications and exciting therapeutic potential.However,the safety of using GNPs in human subjects is an important question that remains unanswered.This study reviews over 20 clinical trials focused on GNP safety and aims to summarize all the clinical studies,completed and ongoing,to identify whether GNPs are safe to use in humans as a therapeutic platform.In these studies,GNPs were implemented as drug delivery devices,for photothermal therapy,and utilized for their intrinsic therapeutic effects by various routes of delivery.These studies revealed no major safety concerns with the use of GNPs;however,the number of trials and total patient number remains limited.Multi-dose,multicenter blinded trials are required to deepen our understanding of the use of GNPs in clinical settings to facilitate translation of this novel,multifaceted therapeutic device.Expanding clinical trials will require collaboration between clinicians,scientists,and biotechnology companies.

Data analysis guidelines for single‑cell RNA‑seq in biomedical studies and clinical applications

The application of single-cell RNA sequencing(scRNA-seq)in biomedical research has advanced our understanding of the pathogenesis of disease and provided valuable insights into new diagnostic and therapeutic strategies.With the expansion of capacity for high-throughput scRNA-seq,including clinical samples,the analysis of these huge volumes of data has become a daunting prospect for researchers entering this field.Here,we review the workflow for typical scRNA-seq data analysis,covering raw data processing and quality control,basic data analysis applicable for almost all scRNA-seq data sets,and advanced data analysis that should be tailored to specific scientific questions.While summarizing the current methods for each analysis step,we also provide an online repository of software and wrapped-up scripts to support the implementation.Recommendations and caveats are pointed out for some specific analysis tasks and approaches.We hope this resource will be helpful to researchers engaging with scRNA-seq,in particular for emerging clinical applications.

Clinical application of SARS-CoV-2 antibody detection and monoclonal antibody therapies against COVID-19

The purpose of this study was to investigate the clinical application of severe acute respiratory distress syndrome coronavirus-2(SARS-CoV-2)specific antibody detection and anti-SARS-CoV-2 specific monoclonal antibodies(mAbs)in the treatment of coronavirus infectious disease 2019(COVID-19).The dynamic changes of SARS-CoV-2 specific antibodies during COVID-19 were studied.Immunoglobulin M(IgM)appeared earlier and lasted for a short time,while immunoglobulin G(IgG)appeared later and lasted longer.IgM tests can be used for early diagnosis of COVID-19,and IgG tests can be used for late diagnosis of COVID-19 and identification of asymptomatic infected persons.The combination of antibody testing and nucleic acid testing,which complement each other,can improve the diagnosis rate of COVID-19.Monoclonal anti-SARS-CoV-2 specific antibodies can be used to treat hospitalized severe and critically ill patients and non-hospitalized mild to moderate COVID-19 patients.COVID-19 convalescent plasma,highly concentrated immunoglobulin,and anti-SARS-CoV-2 specific mAbs are examples of anti-SARS-CoV-2 antibody products.Due to the continuous emergence of mutated strains of the novel coronavirus,especially omicron,its immune escape ability and infectivity are enhanced,making the effects of authorized products reduced or invalid.Therefore,the optimal application of anti-SARS-CoV-2 antibody products(especially anti-SARS-CoV-2 specific mAbs)is more effective in the treatment of COVID-19 and more conducive to patient recovery.

Effect of Combined Application of Organic Farming Aid (OFA) and Inorganic Fertilizers on the Growth and Yield of Maize and Soil Microbial Properties in the Guinea Savannah Agro-Ecological Zone of Ghana

The aim of the study was to evaluate the effect of different rates of organic farming aid (OFA) and inorganic fertilizer on the productivity of maize, chemical and microbial properties of soil for higher economic value. Field experiments were conducted during the 2020 and 2021 cropping seasons at the research fields of CSIR-Savanna Agricultural Research Institute located at Nyankpala in the Guinea savannah agroecology of Ghana. The study consisted of five treatment combinations: full rate of OFA, full rate of NPK, 1/2 OFA + 1/2 NPK, full OFA + 1/2 NPK and a control (no OFA and no NPK) which were arranged in a randomized complete block design with four replications. Analysis of variance indicated significant (P 0.05) treatment and year interaction effect for all the growth parameters except for plant height, leaf area and leaf area index. Apart from hundred seed weight, treatment and year interaction effect for all the yield and yield components was significant (P 0.05). Application of full rate of NPK (90:60:60) resulted in the highest grain yield of 4960 kg·ha-1, however it was statistically similar to those obtained by the combined application of full rate of OFA (250 ml·ha-1) + 1/2 NPK and 1/2 OFA + 1/2 NPK with grain yield of 4856 kg·ha-1 and 4639 kg·ha-1 respectively. There was also a yield advantage of 197. 5%, 191.3%, 178.3 and 79.1% over the control for full NPK rate, full OFA rate + 1/2 NPK rate, 1/2 OFA rate + 1/2 NPK and full OFA rate respectively. Application of full OFA rate + 1/2 NPK enhanced soil basal respiration (evolved CO2) and mineralizable C, implying that, combination of full OFA rate and NPK fertilizer would be necessary to boost soil microbial activity and soil labile nutrient pool (labile C pool). This suggests that combined use of full OFA rate + 1/2 NPK fertilizer can be a better strategic tool for improving soil quality. The highest benefit cost ratios (BCR) of 2.58 and 3.77 were obtained following the application of full OFA rate + 1/2 NPK and 1/2 OFA rate + 1/2 NPK respectively. Hence, it could be concluded that complementary use of OFA and NPK is more profitable than using single inputs (either OFA or NPK). Thus, in promoting technology packages to farmers, development practitioners must carefully consider the complementary of inputs that are cost-effective but economically rewarding.

The Latest Research Progress in the Application of MEWS Scoring System in Clinical Nursing

This paper summarizes the background of the formation of the Modified Early Warning System(MEWS)evaluation system,its current status of clinical teaching applications in different fields of hospitals,and its significance on the medical and nursing career,aiming to provide specific theoretical basis for medical staff and lay a foundation for continuing to carry out related work on MEWS.

Application of Heuristic Method to the Clinical Teaching of Internal Medicine

There is an old saying,“Give a man a fish,and he will eat for a day.Teach a man to fish,and he will eat for the rest of his life.”In clinical teaching,students should not only be taught about diseases,but their intelligence should also be cultivated,along with their analytical,comprehension,and independent learning skills.The ability to solve problems enables students to think independently and acquire knowledge.This is known as the heuristic method of teaching.In this study,we mainly analyze the application value of the heuristic method in the clinical teaching of internal medicine.

内消瘰疬丸加减联合常规西医治疗AIDS合并淋巴结核疗效观察

目的观察内消瘰疬丸加减联合常规西医治疗艾滋病(AIDS)合并淋巴结核患者的临床效果。方法选取2020年4月至2023年6月周口市传染病医院收治的78例AIDS合并淋巴结核患者纳入研究,采用随机抽签法分为常规治疗组和中药联合组各39例。常规治疗组采用高效抗逆转录病毒(HAART)及抗结核药物治疗,中药联合组在此基础上联合内消瘰疬丸加减治疗,均治疗6个月。治疗2个月、6个月后,比较两组患者的抗结核疗效和免疫功能重建疗效,治疗前和治疗2个月、6个月后,比较两组患者的HIV-RNA载量、炎症反应[干扰素-γ释放试验(TB-IGRA)、骨桥蛋白(OPN)、触珠蛋白(HP)]和免疫指标(外周血Th17/Treg、CD4+T淋巴细胞),同时比较两组患者的毒副反应发生情况。结果治疗2个月后,中药联合组患者的抗结核总有效率、免疫功能重建总有效率分别为53.85%、51.28%,与常规治疗组的43.59%、43.59%比较差异均无统计学意义(P>0.05),治疗6个月后,中药联合组患者的抗结核总有效率、免疫功能重建总有效率分别为82.05%、79.49%,明显高于常规治疗组的61.54%、56.41%,差异均有统计学意义(P<0.05);治疗2个月、6个月后,中药联合组患者的HIV-RNA载量为(3.38±0.82)lg10copy/mL、(2.42±0.54)lg10copy/mL,明显低于常规治疗组的(3.95±0.78)lg10copy/mL、(3.05±0.69)lg10copy/mL,差异均有统计学意义(P<0.05);治疗2个月、6个月后,中药联合组患者的TB-IGRA、OPN、HP水平明显低于常规治疗组,差异均有统计学意义(P<0.05);治疗2个月、6个月后,中药联合组患者的Th17水平与Th17/Treg比值分别为(1.69±0.51)%、0.31±0.08和(1.35±0.34)%、0.22±0.05,明显低于常规治疗组的(1.98±0.53)%、0.39±0.09和(1.66±0.40)%、0.30±0.07,Treg、CD4+T水平分别为(5.98±1.14)%、(209.48±19.48)个/μL和(6.26±0.97)%、(219.32±21.24)个/μL,明显高于常规治疗组的(5.37±1.03)%、(197.45±18.65)个/μL和(5.59±1.06)%、(205.68±20.49)个/μL,差异均有统计学意义(P<0.05);中药联合组患者的骨髓抑制、肝功能损伤、肾功能损害、外周神经炎发生率与常规治疗组比较差异均无统计学意义(P>0.05),中药联合组消化道反应发生率为10.26%,明显低于常规治疗组的28.21%,差异有统计学意义(P<0.05)。结论内消瘰疬丸加减联合常规西医治疗能进一步提高抗结核和免疫功能重建疗效,改善患者机体免疫功能,减轻炎症反应,降低HIV-RNA载量,且更为安全。

Progress of Clinical Research on the Treatment of Spinal Disorders by the Tongdu Method

Spinal disorders are diseases caused by congenital, degenerative, postural errors, trauma, and external evils that result in lesions of bones, discs, ligaments, and muscles, thus compressing the nerves. Spine-related diseases include scoliosis, intervertebral disc herniation, intervertebral stenosis, spinal fracture, ankylosing spondylitis, spinal cord injury, spinal tumour, etc., which seriously affect people’s daily life. This paper summarises the relevant studies on the treatment of spinal diseases by Tongzhu therapy. The mechanism of action of Tongzhu therapy in treating spinal diseases is closely related to the control of inflammatory factors, the improvement of local microcirculation, analgesia, and the promotion of nerve repair. This paper describes the application and modern mechanism of Tongzhu therapy in the treatment of spinal diseases, which will provide a direction for future research on Tongzhu therapy.

The first PET scanner made in China and its clinical application

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Research Advances in Pharmacological Action and Clinical Application of Periplaneta americana

Researches on pharmacological action and drug development of Periplane- ta americana have demonstrated that it has wide medicinal value, low side effects and rich resources, having good application prospects in medicine field. This article reviewed the researches on pharmacological action and clinical application of Periplaneta americana in order to provide references for the further research and devel- opment of Periplaneta americana medicines.

Clinical Application and Pharmaceutical Values of Levant Cotton Root

Levant cotton root is a folk traditional herb with a long history in China. This article gave a brief introduction on recent research work related to main com- position, pharmacologic effect and clinical application of levant cotton root and pro- posed existing problems and countermeasures for pharmaceutical industrialization.

HIV/AIDS合并中枢神经系统病变临床分析

目的了解HIV/AIDS合并中枢神经系统病变患者的临床特点,为改善患者预后、提高生存率提供依据。方法以2016年7月~2020年5月昆明医科大学附属传染病医院感染一科收治诊断为HIV/AIDS合并中枢神经系统病变的所有患者为研究对象,收集患者的流行病学信息及病例资料,建立Excel数据库,采用SPSS17.0软件对数据进行分析。结果共有92例HIV/AIDS合并中枢神经系统病变患者纳入本次研究,其中合并隐球菌性脑膜炎占38.05%,结核性脑膜炎占29.35%,弓形虫脑病占13.04%,进行性多灶性白质脑病占7.62%,脑梗塞占6.53%,HIV相关性脑炎占2.17%,鸟分枝杆菌脑膜脑炎占1.08%,病毒性脑膜炎占1.08%。患者CD4^(+)T细胞计数范围在10~838个/μL,中位数86.50个/μL,81.52%的患者CD4^(+)T细胞计数≤200个/μL,55.43%的患者CD4^(+)T细胞计数≤100个/μL。总病死率为21.74%,HIV/AIDS相关中枢神经系统各病种的病死率无统计学差异(P=0.268),不同CD4^(+)T细胞计数患者病死率无统计学差异(P=0.379)。结论HIV/AIDS合并中枢神经系统病变患者病死率高,应注意监测低CD4^(+)T细胞群体神经系统症状,及时诊治提高患者生存率,改善预后。