Objective:To investigate the possibility of Aphanius dispar(A.dispar) acting as intermediate host for Clinostomum complanalum(C.complanalum),from Mehran River,Hormuzgan Province of Iran.Methods:During a biological stu...Objective:To investigate the possibility of Aphanius dispar(A.dispar) acting as intermediate host for Clinostomum complanalum(C.complanalum),from Mehran River,Hormuzgan Province of Iran.Methods:During a biological study of A.dispar in Mehran River,Hormuzgan Province,South of Iran,a total of 97 fish specimens were collected in 24 January 2006.Results: 4 specimens(4.12%) including 1 male and 3 female were found infected with C.complanalum metacercaria.These metacercaria were coiled in the epiderm on the body surface of infected fishes.The infection is known as yellow spot disease.The parasite abundance,intensity and prevalence were 0.03%,1.25%and 4.12%,respectively.The infection was higher in females than males.Conclusions:To the best of our knowledge,this is the first report on occurrence of C. complanatum metacercaria in A.dispar in Iran.展开更多
Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laborator...Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laboratory chicken to obtain ovigerous adult worms.Methods:Live C. punctatus were brought from local fish market of Aligarh, India, dissected and examined on a monthly basis for the presence of C. complanatumcysts. For histochemistry, infected tissue sections with attached cysts were processed for haematoxylene and eosin staining. Cysts were aseptically fed to 4 day old leghorn chicken to obtain adult worms. Mechanically excysted metacercaria and the ovigerous adult worms were stained in carmine to prepare permanent slides.Results:One year survey for the infection of encysted progenetic metacercaria of C.complanatuminC. punctatusrevealed the prevalence, intensity and abundance of 24.7%, 2.27and 0.608, respectively. Histopathology showed heavy infiltration of immune cells at the site of cyst attachment and some tissue damage was also evident. Following feeding to experimental chicken, about 41.07% of the encysted metacercariae were able to excyst and migrate back to bucco-pharyngeal region where they tenaciously attached and fed on blood, and transformed into ovigerous adult worms from 62 hours onwards of post infection.Conclusions:The parasite is potentially pathogenic to the host, and the availability of a suitable intermediate host can be a contributing factor for the occurrence of C. complanatummetacercaria either in the excysted or encysted form, indicating loose host specificity and zoonotic potential.展开更多
In the research of artificial breeding technology of heron,it was found that young herons were easily infected by Clinostomum complanatum which could severely affect the health and growth of heron.In order to explore ...In the research of artificial breeding technology of heron,it was found that young herons were easily infected by Clinostomum complanatum which could severely affect the health and growth of heron.In order to explore the prevention technology of clinostomiasis caused by C.complanatum and promote development of heron artificial breeding technology,we observed epidemic characteristics and clinical symptom of clinostomiasis,and carried out pathological examination and drug treatment trials.The results showed that 20 mg/kg albendaszole had a distinctive effect on clinostomiasis.The comprehensive prevention and control technology against clinostomiasis was further put forward.展开更多
文摘Objective:To investigate the possibility of Aphanius dispar(A.dispar) acting as intermediate host for Clinostomum complanalum(C.complanalum),from Mehran River,Hormuzgan Province of Iran.Methods:During a biological study of A.dispar in Mehran River,Hormuzgan Province,South of Iran,a total of 97 fish specimens were collected in 24 January 2006.Results: 4 specimens(4.12%) including 1 male and 3 female were found infected with C.complanalum metacercaria.These metacercaria were coiled in the epiderm on the body surface of infected fishes.The infection is known as yellow spot disease.The parasite abundance,intensity and prevalence were 0.03%,1.25%and 4.12%,respectively.The infection was higher in females than males.Conclusions:To the best of our knowledge,this is the first report on occurrence of C. complanatum metacercaria in A.dispar in Iran.
基金financially supported by Aligarh Muslim University(grant No.09PHDL128)
文摘Objective:To study the incidence of encysted progenetic metacercariae of Clinostomum complanatum(C. complanatum) in Channa punctatus(C. punctatus), associated histopathology and the experimental infection to laboratory chicken to obtain ovigerous adult worms.Methods:Live C. punctatus were brought from local fish market of Aligarh, India, dissected and examined on a monthly basis for the presence of C. complanatumcysts. For histochemistry, infected tissue sections with attached cysts were processed for haematoxylene and eosin staining. Cysts were aseptically fed to 4 day old leghorn chicken to obtain adult worms. Mechanically excysted metacercaria and the ovigerous adult worms were stained in carmine to prepare permanent slides.Results:One year survey for the infection of encysted progenetic metacercaria of C.complanatuminC. punctatusrevealed the prevalence, intensity and abundance of 24.7%, 2.27and 0.608, respectively. Histopathology showed heavy infiltration of immune cells at the site of cyst attachment and some tissue damage was also evident. Following feeding to experimental chicken, about 41.07% of the encysted metacercariae were able to excyst and migrate back to bucco-pharyngeal region where they tenaciously attached and fed on blood, and transformed into ovigerous adult worms from 62 hours onwards of post infection.Conclusions:The parasite is potentially pathogenic to the host, and the availability of a suitable intermediate host can be a contributing factor for the occurrence of C. complanatummetacercaria either in the excysted or encysted form, indicating loose host specificity and zoonotic potential.
基金Supported by National Spark Program(2011GA780060)
文摘In the research of artificial breeding technology of heron,it was found that young herons were easily infected by Clinostomum complanatum which could severely affect the health and growth of heron.In order to explore the prevention technology of clinostomiasis caused by C.complanatum and promote development of heron artificial breeding technology,we observed epidemic characteristics and clinical symptom of clinostomiasis,and carried out pathological examination and drug treatment trials.The results showed that 20 mg/kg albendaszole had a distinctive effect on clinostomiasis.The comprehensive prevention and control technology against clinostomiasis was further put forward.
