The effect of leaves extracts of Clitoria ternatea Linn against the fish pathogens

Objective:To investigate the antimicrobial activity of Clitoria ternatea(C.ternatea) against the fish pathogens viz.,Pseudomonas aeruginosa(P.aeruginosa),Escherichia coli(E.coli),Klebsiella pneumonia(K.pneumonia),Bacillus subtilis(B.subtilis),Aeromonas formican(A.formicans)s, Aeromonas hydrophila(A.hydrophila) and Streptococcus agalactiae(S.agalactiae )isolated from diseased Tilapia(Oreochromis niloticus).Methods:The extracts of C.ternatea was tested against P.aeruginosa,E.coli,K.pneumonia,B.subtilis,A.formicans,A.hydrophila and S.agalactiae by the agar well diffusion method.Results:Different extracts of C.ternatea showed inhibitory effects against P.aeruginosa,E.coli,K.pneumonia,B.subtilis,A.formicans,A.hydrophila and S. agalactiae.Ethyl acetate extracts of C.ternatea showed maximum of zone of inhibition against A. formicans(18 mm),A.hydrophilia(19 mm),B.subtilis(19 mm) and P.aeruginosa(21 mm) next to that ethanol extract of C.ternatea showed A.formicans(18 mm) and E.coli(14 mm) followed by Acetone extract showed maximum zone of inhibition S.agalactiae(19 mm) and K.pneumonia(17 mm).Conclusions:The antimicrobial activities of all the four plant extracts are comparable and their potential as alternative in the treatment of infectious by these microorganisms was present in the fish.Susceptibility testing is conducted on isolates using drugs selected on the basis of their importance to human medicine and use in fish production.

光温对蝶豆种子萌发的影响

【目的】研究蝶豆种子萌发的最佳条件,为未来人工栽植碟豆和充分利用蝶豆资源提供科学依据。【方法】以蓝色花蝶豆品种为材料,测定不同光照、温度、温汤浸种条件对其种子发芽率、发芽指数、活力指数及幼苗生长的影响。【结果】在0、24、12h光照下,蝶豆种子的发芽率分别为25.56%、23.33%和21.11%,0h光照条件下的种子发芽指数、活力指数最低且幼苗生长差,其余条件下幼苗生长良好;不同恒温条件下蝶豆种子的发芽率由高到底依次为30℃>25℃>20℃>35℃>15℃,在20～30℃内,种子发芽指数和活力指数明显高于15℃和35℃,幼苗长势好;不同温汤浸种条件下蝶豆种子的发芽率由高到低依次为80℃>60℃>50℃>40℃>70℃>CK,浸种温度在40～60℃时,其种子发芽指数和活力指数、发侧根率和出真叶率都较高,幼苗长势好。【结论】温度是决定蝶豆种子萌发的主要因素,20～30℃为蝶豆种子萌发的适宜温度,40～60℃温汤浸种可以提高蝶豆种子的发芽率,而光照对蝶豆种子的萌发影响较小,种子萌发不受有无光照的影响。