The second-instar healthy larvae of Clostera. anastomosis were collected in the artificial woodland of poplar in Shuangcheng Town, Heilongjiang Province, China. The dead larvae of C. anastomosis infected by granulosis...The second-instar healthy larvae of Clostera. anastomosis were collected in the artificial woodland of poplar in Shuangcheng Town, Heilongjiang Province, China. The dead larvae of C. anastomosis infected by granulosis virus （GV） of Clostera anastomosis were grinded to obtain GV. The GV viral pesticide was diluted to seven concentrations, 1.58×10^3PIB·mL^-1, 1,58×10^4PIB·mL^-1, 1.58×10^5PIB·mL^-1 1.58×10^6PIB·mL^-1, 1,58×10^7PIB·mL^-1; 1.58×10^8PIB·mL^-1 and 1.58×10^9PIB·mL^-1 and the fresh poplar leaves were dipped in the seven concentrations liquids to feed the larvae. After nine days the mortality of larvae was investigated. The minimum corrected mortality （7.32%） of larvae was observed at concentrations of 1.58×10^3PIB·mL^-1 and the maximal mortality （97,36%） was observed at concentration of 1.58×10^9PIB·mL^-1. The regression equation between the logarithm of the virus concentration and the mortality was y= 1.946＋0.558x The LC50 was 2.97×10^5PIB·mL^-1. The LT50 for the virus concentration of 1.58×10^5, 1.58×10^6, 1.58×10^7, 1,58×10^58, 1.58×10^9 PIB·mL^-1 were 8.55d, 6.89d, 5.9d, 4.65d, and 4.08d, respectively, shorting gradually with the concentration increasing, It is concluded that the toxicity of Clostera anastomosis GV is very strong and as a kind of insecticides it has big potential in practical application.展开更多
A modified guanidinium isothiocyanate method was used to extract total RNA from two forest insect species Clostera anastomosis and Saperda populnea. The integrity of RNA was demonstrated by the methods of gel electrop...A modified guanidinium isothiocyanate method was used to extract total RNA from two forest insect species Clostera anastomosis and Saperda populnea. The integrity of RNA was demonstrated by the methods of gel electrophoresis and cDNA analysis. Typical A260/ A280 absorbance ratio of the total RNA was in range of 1.8 to 2.0. The size of double strand cDNAs obtained by RT-PCR was more than 2 kb, which indicated that intact mRNA was obtained. The fragments of β-actin and chitinase gene from the RNA of C. anastomosis were obtained by RT-PCR, which indicated that the RNA could be used for other molecular operation. By this procedure, RNAs could be extracted and analyzed by electrophoresis from at least 8 samples within 4 hours. These results showed that this method was time- and cost-saving and effective.展开更多
There is no effective treatment for hemiplegia after hypertensive intracerebral hemorrhage.Considering that the branches of L4 nerve roots in the lumbar plexus root control the movement of the lower extremity anterior...There is no effective treatment for hemiplegia after hypertensive intracerebral hemorrhage.Considering that the branches of L4 nerve roots in the lumbar plexus root control the movement of the lower extremity anterior and posterior muscles,we investigated a potential method of nerve repair using the L4 nerve roots.Rat models of hindlimb hemiplegia after a hypertensive intracerebral hemorrhage were established by injecting autogenous blood into the posterior limb of internal capsule.The L4 nerve root on the healthy side of model rats was transferred and then anastomosed with the L4 nerve root on the affected side to drive the extensor and flexor muscles of the hindlimbs.We investigated whether this method can restore the flexible movement of the hindlimbs of paralyzed rats after hypertensive intracerebral hemorrhage.In a beam-walking test and ladder rung walking task,model rats exhibited an initial high number of slips,but improved in accuracy on the paretic side over time.At 17 weeks after surgery,rats gained approximately 58.2%accuracy from baseline performance and performed ankle motions on the paretic side.At 9 weeks after surgery,a retrograde tracing test showed a large number of fluoro-gold-labeled motoneurons in the left anterior horn of the spinal cord that supports the L4-to-L4 nerve roots.In addition,histological and ultramicrostructural findings showed axon regeneration of motoneurons in the anterior horn of the spinal cord.Electromyography and paw print analysis showed that denervated hindlimb muscles regained reliable innervation and walking coordination improved.These findings suggest that the L4-to-L4 nerve root transfer method for the treatment of hindlimb hemiplegia after hypertensive intracerebral hemorrhage can improve the locomotion of hindlimb major joints,particularly of the distal ankle.Findings from study support that the L4-to-L4 nerve root transfer method can effectively repair the hindlimb hemiplegia after hypertensive intracerebral hemorrhage.All animal experiments were approved by the Animal Ethics Committee of the First Affiliated Hospital of Nanjing Medical University(No.IACUC-1906009)in June 2019.展开更多
Several phytoecdysteroids have been found in the extracts of A. multiflora and A.linearifolia using HPLC analysis. Those were 20-OH ecdysone, cyasterone, 3-acetate-20-OH ecdysone, 2-acetate-20-OH ecdysone, qugalactone...Several phytoecdysteroids have been found in the extracts of A. multiflora and A.linearifolia using HPLC analysis. Those were 20-OH ecdysone, cyasterone, 3-acetate-20-OH ecdysone, 2-acetate-20-OH ecdysone, qugalactone. We found out that those phytoecdysteroids could lead the second instar larva of Clostera anastomosis and Stipnotia candida to death. The modified mortality rate was from 44.64% to 96.24%. Those extracts could signiticantly reduce the amount of nymph produced by the female Tuberolachnus salignus, The number of the nymph produced after treating with those extracts was only 20% to 70% of that produced by the untreated group. The death rate of the newly produced nymph which treated was among 33.33% to 58.82%. It was much higher than the death rate of the control group (1l7.57%).展开更多
文摘The second-instar healthy larvae of Clostera. anastomosis were collected in the artificial woodland of poplar in Shuangcheng Town, Heilongjiang Province, China. The dead larvae of C. anastomosis infected by granulosis virus （GV） of Clostera anastomosis were grinded to obtain GV. The GV viral pesticide was diluted to seven concentrations, 1.58×10^3PIB·mL^-1, 1,58×10^4PIB·mL^-1, 1.58×10^5PIB·mL^-1 1.58×10^6PIB·mL^-1, 1,58×10^7PIB·mL^-1; 1.58×10^8PIB·mL^-1 and 1.58×10^9PIB·mL^-1 and the fresh poplar leaves were dipped in the seven concentrations liquids to feed the larvae. After nine days the mortality of larvae was investigated. The minimum corrected mortality （7.32%） of larvae was observed at concentrations of 1.58×10^3PIB·mL^-1 and the maximal mortality （97,36%） was observed at concentration of 1.58×10^9PIB·mL^-1. The regression equation between the logarithm of the virus concentration and the mortality was y= 1.946＋0.558x The LC50 was 2.97×10^5PIB·mL^-1. The LT50 for the virus concentration of 1.58×10^5, 1.58×10^6, 1.58×10^7, 1,58×10^58, 1.58×10^9 PIB·mL^-1 were 8.55d, 6.89d, 5.9d, 4.65d, and 4.08d, respectively, shorting gradually with the concentration increasing, It is concluded that the toxicity of Clostera anastomosis GV is very strong and as a kind of insecticides it has big potential in practical application.
基金This study was supported by Key Project of Harbin City (2005AA6CN074)
文摘A modified guanidinium isothiocyanate method was used to extract total RNA from two forest insect species Clostera anastomosis and Saperda populnea. The integrity of RNA was demonstrated by the methods of gel electrophoresis and cDNA analysis. Typical A260/ A280 absorbance ratio of the total RNA was in range of 1.8 to 2.0. The size of double strand cDNAs obtained by RT-PCR was more than 2 kb, which indicated that intact mRNA was obtained. The fragments of β-actin and chitinase gene from the RNA of C. anastomosis were obtained by RT-PCR, which indicated that the RNA could be used for other molecular operation. By this procedure, RNAs could be extracted and analyzed by electrophoresis from at least 8 samples within 4 hours. These results showed that this method was time- and cost-saving and effective.
基金the National Natural Science Foundation of China,No.81171147(to LXL)“Key Medical Talents of Qiangwei Project”Research Foundation of Health Department of Jiangsu Province,No.ZDRCA2016010(to LXL)+3 种基金“Xingwei Project”Key Personal Medical Research Foundation of Health Department of Jiangsu Province,No.RC201156(to LXL)Jiangsu Province’s Key Discipline of Medicine,No.XK201117(to LXL)the Priority Academic Program Development of Jiangsu Higher Education Institutions,PAPD(to LXL)the Natural Science Foundation of Jiangsu Province,No.BK20171064(to BSH).
文摘There is no effective treatment for hemiplegia after hypertensive intracerebral hemorrhage.Considering that the branches of L4 nerve roots in the lumbar plexus root control the movement of the lower extremity anterior and posterior muscles,we investigated a potential method of nerve repair using the L4 nerve roots.Rat models of hindlimb hemiplegia after a hypertensive intracerebral hemorrhage were established by injecting autogenous blood into the posterior limb of internal capsule.The L4 nerve root on the healthy side of model rats was transferred and then anastomosed with the L4 nerve root on the affected side to drive the extensor and flexor muscles of the hindlimbs.We investigated whether this method can restore the flexible movement of the hindlimbs of paralyzed rats after hypertensive intracerebral hemorrhage.In a beam-walking test and ladder rung walking task,model rats exhibited an initial high number of slips,but improved in accuracy on the paretic side over time.At 17 weeks after surgery,rats gained approximately 58.2%accuracy from baseline performance and performed ankle motions on the paretic side.At 9 weeks after surgery,a retrograde tracing test showed a large number of fluoro-gold-labeled motoneurons in the left anterior horn of the spinal cord that supports the L4-to-L4 nerve roots.In addition,histological and ultramicrostructural findings showed axon regeneration of motoneurons in the anterior horn of the spinal cord.Electromyography and paw print analysis showed that denervated hindlimb muscles regained reliable innervation and walking coordination improved.These findings suggest that the L4-to-L4 nerve root transfer method for the treatment of hindlimb hemiplegia after hypertensive intracerebral hemorrhage can improve the locomotion of hindlimb major joints,particularly of the distal ankle.Findings from study support that the L4-to-L4 nerve root transfer method can effectively repair the hindlimb hemiplegia after hypertensive intracerebral hemorrhage.All animal experiments were approved by the Animal Ethics Committee of the First Affiliated Hospital of Nanjing Medical University(No.IACUC-1906009)in June 2019.
文摘Several phytoecdysteroids have been found in the extracts of A. multiflora and A.linearifolia using HPLC analysis. Those were 20-OH ecdysone, cyasterone, 3-acetate-20-OH ecdysone, 2-acetate-20-OH ecdysone, qugalactone. We found out that those phytoecdysteroids could lead the second instar larva of Clostera anastomosis and Stipnotia candida to death. The modified mortality rate was from 44.64% to 96.24%. Those extracts could signiticantly reduce the amount of nymph produced by the female Tuberolachnus salignus, The number of the nymph produced after treating with those extracts was only 20% to 70% of that produced by the untreated group. The death rate of the newly produced nymph which treated was among 33.33% to 58.82%. It was much higher than the death rate of the control group (1l7.57%).
