Purpose – Straightness measurement of rail weld joint is of essential importance to railway maintenance. Dueto the lack of efficient measurement equipment, there has been limited in-depth research on rail weld joint ...Purpose – Straightness measurement of rail weld joint is of essential importance to railway maintenance. Dueto the lack of efficient measurement equipment, there has been limited in-depth research on rail weld joint with a5-m wavelength range, leaving a significant knowledge gap in this field.Design/methodology/approach – In this study, the authors used the well-established inertial referencemethod (IR-method), and the state-of-the-art multi-point chord reference method (MCR-method). Two methodshave been applied in different types of rail straightness measurement trollies, respectively. These instrumentswere tested in a high-speed rail section within a certain region of China. The test results were ultimatelyvalidated through using traditional straightedge and feeler gauge methods as reference data to evaluate the railweld joint straightness within the 5-m wavelength range.Findings – The research reveals that IR-method and MCR-method produce reasonably similar measurementresults for wavelengths below 1 m. However, MCR-method outperforms IR-method in terms of accuracy forwavelengths exceeding 3 m. Furthermore, it was observed that IR-method, while operating at a slower speed,carries the risk of derailing and is incapable of detecting rail weld joints and low joints within the track.Originality/value – The research compare two methods’ measurement effects in a longer wavelength rangeand demonstrate the superiority of MCR-method.展开更多
The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristic...The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristics of the operated patients, to determine the main pathologies encountered and to evaluate qualitatively the result of the treatment. In order to improve performance, and the quality of care, and to identify common pathologies in the surgical department, we undertook a retrospective study on surgical activities from January 2009 to December 2010. At the end of this study, out of 474 men and 187 women (equal sex ratio 2.53);we were able to determine the frequency of surgical pathologies. Farmers, housewives and pupils/students were the most represented with 25.9% respectively;20% and 13.3%. The most frequently observed pathologies were wall hernia (44.8%), prostate adenoma (12%) and acute appendicitis (10.5%). The average length of hospitalization was 3.43 days. Infectious complications affected 25 patients (3.8% of cases) and a death rate of 0.45% (i.e. 3 patients). The average cost of care was 53,500 FCFA. Indeed, the reality of surgical practice in health centers was not the same because of the level of skills of practicing surgeons.展开更多
Channel equalization plays a pivotal role within the reconstruction phase of passive radar reference signals.In the context of reconstructing digital terrestrial multimedia broadcasting(DTMB)signals for low-slow-small...Channel equalization plays a pivotal role within the reconstruction phase of passive radar reference signals.In the context of reconstructing digital terrestrial multimedia broadcasting(DTMB)signals for low-slow-small(LSS)target detection,a novel frequency domain block joint equalization algorithm is presented in this article.From the DTMB signal frame structure and channel multipath transmission characteristics,this article adopts a unconventional approach where the delay and frame structure of each DTMB signal frame are reconfigured to create a circular convolution block,facilitating concurrent fast Fourier transform(FFT)calculations.Following equalization,an inverse fast Fourier transform(IFFT)-based joint output and subsequent data reordering are executed to finalize the equalization process for the DTMB signal.Simulation and measured data confirm that this algorithm outperforms conventional techniques by reducing signal errors rate and enhancing real-time processing.In passive radar LSS detection,it effectively suppresses multipath and noise through frequency domain equalization,reducing false alarms and improving the capabilities of weak target detection.展开更多
It is still a huge challenge for traditional Pareto-dominatedmany-objective optimization algorithms to solve manyobjective optimization problems because these algorithms hardly maintain the balance between convergence...It is still a huge challenge for traditional Pareto-dominatedmany-objective optimization algorithms to solve manyobjective optimization problems because these algorithms hardly maintain the balance between convergence and diversity and can only find a group of solutions focused on a small area on the Pareto front,resulting in poor performance of those algorithms.For this reason,we propose a reference vector-assisted algorithmwith an adaptive niche dominance relation,for short MaOEA-AR.The new dominance relation forms a niche based on the angle between candidate solutions.By comparing these solutions,the solutionwith the best convergence is found to be the non-dominated solution to improve the selection pressure.In reproduction,a mutation strategy of k-bit crossover and hybrid mutation is used to generate high-quality offspring.On 23 test problems with up to 15-objective,we compared the proposed algorithm with five state-of-the-art algorithms.The experimental results verified that the proposed algorithm is competitive.展开更多
Inertial reference system is one of the airborne equipment.According to the requirements of SAE ARP4754A Guidelines for Development of Civil Aircraft and Systems,MC9 equipment qualification test is needed to verify th...Inertial reference system is one of the airborne equipment.According to the requirements of SAE ARP4754A Guidelines for Development of Civil Aircraft and Systems,MC9 equipment qualification test is needed to verify that the inertial reference system can perform reservation function under specified service conditions.That is,the inertial reference system shall pass certain environmental tests specified in DO⁃160G.Some tests are faced with the problem that the test equipment should have the function requirements of isolation protection and load simulation.Therefore,a kind of test equipment which can provide isolation protection and simulate load function in the test is designed.展开更多
为了探究高速空气燃料热喷涂(activated combustion-high velocity air fuel,AC-HVAF)过程中喷涂粒子撞击基材后的沉积特性。采用AC-HVAF热喷涂技术在AZ80镁合金基体上沉积WC-10Co-4Cr硬质涂层。通过离散沉积实验获得薄层沉积粒子,探讨...为了探究高速空气燃料热喷涂(activated combustion-high velocity air fuel,AC-HVAF)过程中喷涂粒子撞击基材后的沉积特性。采用AC-HVAF热喷涂技术在AZ80镁合金基体上沉积WC-10Co-4Cr硬质涂层。通过离散沉积实验获得薄层沉积粒子,探讨各种沉积形貌的种类、形成原因、结合机制及射流中粒子的径向和轴向分布。结果表明:在AC-HVAF粒子沉积过程中,嵌入型沉积为主要的沉积形貌,同时包含少量的破碎型与空腔型沉积粒子。在涂层的形成过程中,嵌入型沉积对涂层/基体结合性能起重要作用;空腔型沉积的小颗粒及破碎型沉积的大颗粒是造成沉积效率下降的主要原因。喷涂粒子主要集中在射流中心,越靠近射流边缘,空腔型沉积粒子越多,最终导致AC-HVAF粒子射流呈现出空间分布特征。展开更多
The importance of developing high-resolution seismic models to improve understanding of tectonic processes and enhance seismic hazard mitigation programs,along with the rapid expansion of seismic coverage in China,cal...The importance of developing high-resolution seismic models to improve understanding of tectonic processes and enhance seismic hazard mitigation programs,along with the rapid expansion of seismic coverage in China,called for a seismological reference model to be established in China.The China Seismological Reference Model(CSRM)project was initiated by the National Natural Science Foundation of China with two primary goals:(1)the CSRM would serve as a primary source for the current state of seismological research in China,and(2)the seismic data and constraints used to construct the CSRM would be used as a backbone open-access cyberinfrastructure for future research in seismology.The CSRM project was also intended to promote data exchange and scientific collaboration in seismology in China.Accordingly,two parallel efforts of the project are being pursued:(1)construction of the CSRM,and(2)development of a CSRM product center.The CSRM is jointly constrained by various types of seismic constraints extracted from the seismic data recorded at 4511 seismic stations in continental China following a top-down approach,with the seismic structures in the shallower part of the Earth constrained first.Construction of the CSRM involves three preparation steps:(1)building datasets of various seismic constraints from the seismic data,(2)developing a method to incorporate the constraints of surface wave observations from regional earthquakes into the inversion of the seismic structure,and(3)constructing high-resolution pre-CSRM seismic models of the velocity structure in the shallow crust and the Pn-velocity structure in the uppermost mantle.In the final process,the CSRM will be constructed by jointly inverting all the seismic constraints using the pre-CSRM models as starting models or a priori structures.The CSRM product center(http://chinageorefmodel.org)archives and distributes three types of products:CSRM models,the Level 1 original seismic data used to extract seismic constraints in the construction of the CSRM,and Level 2 data on the seismic constraints derived from the Level 1 data and the inferred earthquake parameters in the construction of the CSRM.The CSRM product center has archived 141 TB of Level 1 data from 1120 permanent broadband stations in the China Seismic Network Center and 3391 temporary stations from various institutions and data centers around the world,as well as 140 GB of Level 2 data on various seismic constraints and inferred event parameters from the construction of the CSRM.The CSRM is expected to provide significant insights into the composition and tectonic dynamics in continental China and to enhance the capability of various seismic hazard mitigation programs in China from near real-time rapid determination of earthquake parameters to an earthquake early warning system.The CSRM could also provide guidance for focuses in future seismological research and the design of future active and passive seismic experiments in China.Several focuses are suggested for future seismological research in China,along with the building of a national cyberinfrastructure to sustain and expand the operations of the CSRM project.展开更多
Quantitative real-time PCR(qRT-PCR)has been widely used for gene expression analysis,and selection of reference genes is a key point to obtain accurate results.To find out optimal reference genes for qRT-PCR in Manila...Quantitative real-time PCR(qRT-PCR)has been widely used for gene expression analysis,and selection of reference genes is a key point to obtain accurate results.To find out optimal reference genes for qRT-PCR in Manila clam Ruditapes philippinarum in response to hypoxia,different tissues were used and compared to evaluate the stability of candidate reference genes under low oxygen stress(DO 0.5mgL^(−1) and DO 2.0mgL^(−1))and normal condition(DO 7.5mgL^(−1)).Seven candidate reference genes were selected to evaluate the stability of their expression levels.The reference genes were evaluated by Delta Ct,BestKeeper,NormFinder and geNorm,and then screened by RefFinder calculation.Under hypoxic stress of 0.5mgL^(−1),the most suitable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were TUB and HIS,respectively.For hypoxic stress of 2.0mgL^(−1),the most stable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were RPS23 and EF1A,respectively.At the normal condition,HIS and EF1A were identified as the optimal internal reference genes in gill and hepatopancreas respectively,and GFRP2 was the best internal reference gene for axe foot and adductor muscle.The present findings will provide important basis for the selection of reference genes for qRT-PCR analysis of gene expression level in bivalves under hypoxic stress,which might be helpful for the analysis of other molluscs too.展开更多
The high-precision terrestrial reference frame,as the spatial benchmark for geodesy,is an important national infrastructure.However,due to the influence of nonlinear factors related to geophysical phenomena,the overal...The high-precision terrestrial reference frame,as the spatial benchmark for geodesy,is an important national infrastructure.However,due to the influence of nonlinear factors related to geophysical phenomena,the overall maintenance accuracy of the ITRF framework is still at the centimeter level.Therefore,accurately characterizing the true trajectories of linear motion,nonlinear motion,and geocentric motion of the reference station is the key to achieve the construction and maintenance technology of a millimeter level terrestrial reference framework.Based on long-term global and regional GNSS observation data,more Chinese geodesy scientists devoted much efforts to the maintenance of millimeter-level geodetic reference framework.The main contributions of this work included the followings:①Dynamic maintenance of millimeter-level terrestrial reference frame;②Research progress on the method of maintenance of regional reference frame based on GNSS;③The progress of CGCS2000 frame maintenance in millimeter level accuracy;④Reprocessing and reanalysis of two-decade GNSS observation in continental China;⑤Research on current GNSS velocity field model and deformation in Chinese mainland;⑥The preliminary realization and evaluation of CTRF2020.展开更多
Context: Vitamin E is a powerful antioxidant and plays an important role in human reproduction. However, micronutrient deficiency is a major public health problem, particularly in developing countries. This study aime...Context: Vitamin E is a powerful antioxidant and plays an important role in human reproduction. However, micronutrient deficiency is a major public health problem, particularly in developing countries. This study aimed to establish reference intervals (RIs) for vitamin E in black Congolese people of childbearing age using an ELISA method to provide a reference for clinically assessing vitamin E status. Methods: A total of 127 healthy people between the ages of 20 and 42 who underwent check-ups were randomly selected for the study. ELISA method measured the level of vitamin E. The effect of gender on vitamin E level was assessed, and RI was established using a parametric approach. Results: Women showed significantly higher levels of vitamin E than men (p = 0.01). The RI of vitamin E in people of childbearing age was 3.71 to 13.72, 4.52 to 14.64, and 4.17 to 13.52 mg/L, respectively, for the whole population, women and men. Conclusion: Using an ELISA method, this study established RI for vitamin E in the black Congolese population of childbearing age. We also found that women had significantly higher vitamin E levels than men. The results could provide a scientific basis for interpreting vitamin status in people of childbearing age in our setting.展开更多
Background:Acute pancreatitis(AP)is a severe disorder that leads to high morbidity and mortality.Appropriate reference genes are important for gene analysis in AP.This study sought to study the expression stability of...Background:Acute pancreatitis(AP)is a severe disorder that leads to high morbidity and mortality.Appropriate reference genes are important for gene analysis in AP.This study sought to study the expression stability of several reference genes in the golden Syrian hamster,a model of AP.Methods:AP was induced in golden Syrian hamster by intraperitoneal injection of ethanol(1.35 g/kg)and palmitoleic acid(2 mg/kg).The expression of candidate genes,including Actb,Gapdh,Eef2,Ywhaz,Rps18,Hprt1,Tubb,Rpl13a,Nono,and B2m,in hamster pancreas at different time points(1,3,6,9,and 24 h)posttreatment was analyzed using quantitative polymerase chain reaction.The expression stability of these genes was calculated using Best Keeper,Comprehensive Delta CT,Norm Finder,and ge Norm algorithms and Ref Finder software.Results:Our results show that the expression of these reference genes fluctuated during AP,of which Ywhaz and Gapdh were the most stable genes,whereas Tubb,Eef2,and Actb were the least stable genes.Furthermore,these genes were used to normalize the expression of TNF-αmessenger ribonucleic acid in inflamed pancreas.Conclusions:In conclusion,Ywhaz and Gapdh were suitable reference genes for gene expression analysis in AP induced in Syrian hamster.展开更多
文摘Purpose – Straightness measurement of rail weld joint is of essential importance to railway maintenance. Dueto the lack of efficient measurement equipment, there has been limited in-depth research on rail weld joint with a5-m wavelength range, leaving a significant knowledge gap in this field.Design/methodology/approach – In this study, the authors used the well-established inertial referencemethod (IR-method), and the state-of-the-art multi-point chord reference method (MCR-method). Two methodshave been applied in different types of rail straightness measurement trollies, respectively. These instrumentswere tested in a high-speed rail section within a certain region of China. The test results were ultimatelyvalidated through using traditional straightedge and feeler gauge methods as reference data to evaluate the railweld joint straightness within the 5-m wavelength range.Findings – The research reveals that IR-method and MCR-method produce reasonably similar measurementresults for wavelengths below 1 m. However, MCR-method outperforms IR-method in terms of accuracy forwavelengths exceeding 3 m. Furthermore, it was observed that IR-method, while operating at a slower speed,carries the risk of derailing and is incapable of detecting rail weld joints and low joints within the track.Originality/value – The research compare two methods’ measurement effects in a longer wavelength rangeand demonstrate the superiority of MCR-method.
文摘The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristics of the operated patients, to determine the main pathologies encountered and to evaluate qualitatively the result of the treatment. In order to improve performance, and the quality of care, and to identify common pathologies in the surgical department, we undertook a retrospective study on surgical activities from January 2009 to December 2010. At the end of this study, out of 474 men and 187 women (equal sex ratio 2.53);we were able to determine the frequency of surgical pathologies. Farmers, housewives and pupils/students were the most represented with 25.9% respectively;20% and 13.3%. The most frequently observed pathologies were wall hernia (44.8%), prostate adenoma (12%) and acute appendicitis (10.5%). The average length of hospitalization was 3.43 days. Infectious complications affected 25 patients (3.8% of cases) and a death rate of 0.45% (i.e. 3 patients). The average cost of care was 53,500 FCFA. Indeed, the reality of surgical practice in health centers was not the same because of the level of skills of practicing surgeons.
文摘Channel equalization plays a pivotal role within the reconstruction phase of passive radar reference signals.In the context of reconstructing digital terrestrial multimedia broadcasting(DTMB)signals for low-slow-small(LSS)target detection,a novel frequency domain block joint equalization algorithm is presented in this article.From the DTMB signal frame structure and channel multipath transmission characteristics,this article adopts a unconventional approach where the delay and frame structure of each DTMB signal frame are reconfigured to create a circular convolution block,facilitating concurrent fast Fourier transform(FFT)calculations.Following equalization,an inverse fast Fourier transform(IFFT)-based joint output and subsequent data reordering are executed to finalize the equalization process for the DTMB signal.Simulation and measured data confirm that this algorithm outperforms conventional techniques by reducing signal errors rate and enhancing real-time processing.In passive radar LSS detection,it effectively suppresses multipath and noise through frequency domain equalization,reducing false alarms and improving the capabilities of weak target detection.
基金supported by the National Natural Science Foundation of China(Grant No.61976101)the University Natural Science Research Project of Anhui Province(Grant No.2023AH040056)+4 种基金the Natural Science Research Project of Anhui Province(Graduate Research Project,Grant No.YJS20210463)the Funding Plan for Scientic Research Activities of Academic and Technical Leaders and Reserve Candidates in Anhui Province(Grant No.2021H264)the Top Talent Project of Disciplines(Majors)in Colleges and Universities in Anhui Province(Grant No.gxbjZD2022021)the University Synergy Innovation Program of Anhui Province,China(GXXT-2022-033)supported by the Innovation Fund for Postgraduates of Huaibei Normal University(Grant Nos.cx2022041,yx2021023,CX2023043).
文摘It is still a huge challenge for traditional Pareto-dominatedmany-objective optimization algorithms to solve manyobjective optimization problems because these algorithms hardly maintain the balance between convergence and diversity and can only find a group of solutions focused on a small area on the Pareto front,resulting in poor performance of those algorithms.For this reason,we propose a reference vector-assisted algorithmwith an adaptive niche dominance relation,for short MaOEA-AR.The new dominance relation forms a niche based on the angle between candidate solutions.By comparing these solutions,the solutionwith the best convergence is found to be the non-dominated solution to improve the selection pressure.In reproduction,a mutation strategy of k-bit crossover and hybrid mutation is used to generate high-quality offspring.On 23 test problems with up to 15-objective,we compared the proposed algorithm with five state-of-the-art algorithms.The experimental results verified that the proposed algorithm is competitive.
文摘Inertial reference system is one of the airborne equipment.According to the requirements of SAE ARP4754A Guidelines for Development of Civil Aircraft and Systems,MC9 equipment qualification test is needed to verify that the inertial reference system can perform reservation function under specified service conditions.That is,the inertial reference system shall pass certain environmental tests specified in DO⁃160G.Some tests are faced with the problem that the test equipment should have the function requirements of isolation protection and load simulation.Therefore,a kind of test equipment which can provide isolation protection and simulate load function in the test is designed.
文摘为了探究高速空气燃料热喷涂(activated combustion-high velocity air fuel,AC-HVAF)过程中喷涂粒子撞击基材后的沉积特性。采用AC-HVAF热喷涂技术在AZ80镁合金基体上沉积WC-10Co-4Cr硬质涂层。通过离散沉积实验获得薄层沉积粒子,探讨各种沉积形貌的种类、形成原因、结合机制及射流中粒子的径向和轴向分布。结果表明:在AC-HVAF粒子沉积过程中,嵌入型沉积为主要的沉积形貌,同时包含少量的破碎型与空腔型沉积粒子。在涂层的形成过程中,嵌入型沉积对涂层/基体结合性能起重要作用;空腔型沉积的小颗粒及破碎型沉积的大颗粒是造成沉积效率下降的主要原因。喷涂粒子主要集中在射流中心,越靠近射流边缘,空腔型沉积粒子越多,最终导致AC-HVAF粒子射流呈现出空间分布特征。
基金the NSFC has funded 40 research grants for studying the seismic structure beneath continental China, including a grant NSFC42250201 focusing on the CSRM project
文摘The importance of developing high-resolution seismic models to improve understanding of tectonic processes and enhance seismic hazard mitigation programs,along with the rapid expansion of seismic coverage in China,called for a seismological reference model to be established in China.The China Seismological Reference Model(CSRM)project was initiated by the National Natural Science Foundation of China with two primary goals:(1)the CSRM would serve as a primary source for the current state of seismological research in China,and(2)the seismic data and constraints used to construct the CSRM would be used as a backbone open-access cyberinfrastructure for future research in seismology.The CSRM project was also intended to promote data exchange and scientific collaboration in seismology in China.Accordingly,two parallel efforts of the project are being pursued:(1)construction of the CSRM,and(2)development of a CSRM product center.The CSRM is jointly constrained by various types of seismic constraints extracted from the seismic data recorded at 4511 seismic stations in continental China following a top-down approach,with the seismic structures in the shallower part of the Earth constrained first.Construction of the CSRM involves three preparation steps:(1)building datasets of various seismic constraints from the seismic data,(2)developing a method to incorporate the constraints of surface wave observations from regional earthquakes into the inversion of the seismic structure,and(3)constructing high-resolution pre-CSRM seismic models of the velocity structure in the shallow crust and the Pn-velocity structure in the uppermost mantle.In the final process,the CSRM will be constructed by jointly inverting all the seismic constraints using the pre-CSRM models as starting models or a priori structures.The CSRM product center(http://chinageorefmodel.org)archives and distributes three types of products:CSRM models,the Level 1 original seismic data used to extract seismic constraints in the construction of the CSRM,and Level 2 data on the seismic constraints derived from the Level 1 data and the inferred earthquake parameters in the construction of the CSRM.The CSRM product center has archived 141 TB of Level 1 data from 1120 permanent broadband stations in the China Seismic Network Center and 3391 temporary stations from various institutions and data centers around the world,as well as 140 GB of Level 2 data on various seismic constraints and inferred event parameters from the construction of the CSRM.The CSRM is expected to provide significant insights into the composition and tectonic dynamics in continental China and to enhance the capability of various seismic hazard mitigation programs in China from near real-time rapid determination of earthquake parameters to an earthquake early warning system.The CSRM could also provide guidance for focuses in future seismological research and the design of future active and passive seismic experiments in China.Several focuses are suggested for future seismological research in China,along with the building of a national cyberinfrastructure to sustain and expand the operations of the CSRM project.
基金supported by research grants from the Science and Technology Innovation Program of the Laoshan Laboratory(No.LSKJ202203803)the National Natural Science Foundation of China(No.32273107)+2 种基金supported by the Central Public-Interest Scientific Institution Basal Research Fund,Yellow Sea Fisheries Research Institute,CAFS(No.20603022022001)the project of Putian Science and Technology Department(No.2021NJJ002)the Shinan District Science and Technology Plan Project(No.2022-2-026-ZH).
文摘Quantitative real-time PCR(qRT-PCR)has been widely used for gene expression analysis,and selection of reference genes is a key point to obtain accurate results.To find out optimal reference genes for qRT-PCR in Manila clam Ruditapes philippinarum in response to hypoxia,different tissues were used and compared to evaluate the stability of candidate reference genes under low oxygen stress(DO 0.5mgL^(−1) and DO 2.0mgL^(−1))and normal condition(DO 7.5mgL^(−1)).Seven candidate reference genes were selected to evaluate the stability of their expression levels.The reference genes were evaluated by Delta Ct,BestKeeper,NormFinder and geNorm,and then screened by RefFinder calculation.Under hypoxic stress of 0.5mgL^(−1),the most suitable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were TUB and HIS,respectively.For hypoxic stress of 2.0mgL^(−1),the most stable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were RPS23 and EF1A,respectively.At the normal condition,HIS and EF1A were identified as the optimal internal reference genes in gill and hepatopancreas respectively,and GFRP2 was the best internal reference gene for axe foot and adductor muscle.The present findings will provide important basis for the selection of reference genes for qRT-PCR analysis of gene expression level in bivalves under hypoxic stress,which might be helpful for the analysis of other molluscs too.
基金National Natural Science Foundation of China(Nos.42274044,41974010,42330113,41804018)。
文摘The high-precision terrestrial reference frame,as the spatial benchmark for geodesy,is an important national infrastructure.However,due to the influence of nonlinear factors related to geophysical phenomena,the overall maintenance accuracy of the ITRF framework is still at the centimeter level.Therefore,accurately characterizing the true trajectories of linear motion,nonlinear motion,and geocentric motion of the reference station is the key to achieve the construction and maintenance technology of a millimeter level terrestrial reference framework.Based on long-term global and regional GNSS observation data,more Chinese geodesy scientists devoted much efforts to the maintenance of millimeter-level geodetic reference framework.The main contributions of this work included the followings:①Dynamic maintenance of millimeter-level terrestrial reference frame;②Research progress on the method of maintenance of regional reference frame based on GNSS;③The progress of CGCS2000 frame maintenance in millimeter level accuracy;④Reprocessing and reanalysis of two-decade GNSS observation in continental China;⑤Research on current GNSS velocity field model and deformation in Chinese mainland;⑥The preliminary realization and evaluation of CTRF2020.
文摘Context: Vitamin E is a powerful antioxidant and plays an important role in human reproduction. However, micronutrient deficiency is a major public health problem, particularly in developing countries. This study aimed to establish reference intervals (RIs) for vitamin E in black Congolese people of childbearing age using an ELISA method to provide a reference for clinically assessing vitamin E status. Methods: A total of 127 healthy people between the ages of 20 and 42 who underwent check-ups were randomly selected for the study. ELISA method measured the level of vitamin E. The effect of gender on vitamin E level was assessed, and RI was established using a parametric approach. Results: Women showed significantly higher levels of vitamin E than men (p = 0.01). The RI of vitamin E in people of childbearing age was 3.71 to 13.72, 4.52 to 14.64, and 4.17 to 13.52 mg/L, respectively, for the whole population, women and men. Conclusion: Using an ELISA method, this study established RI for vitamin E in the black Congolese population of childbearing age. We also found that women had significantly higher vitamin E levels than men. The results could provide a scientific basis for interpreting vitamin status in people of childbearing age in our setting.
基金China Postdoctoral Science Foundation,Grant/Award Number:2021T140184Program for Science Technology Innovation Talents in Universities of Henan Province,Grant/Award Number:23HASTIT045Scientific Research of Traditional Chinese Medicine Specialized in Henan Province,Grant/Award Number:2022ZY1172。
文摘Background:Acute pancreatitis(AP)is a severe disorder that leads to high morbidity and mortality.Appropriate reference genes are important for gene analysis in AP.This study sought to study the expression stability of several reference genes in the golden Syrian hamster,a model of AP.Methods:AP was induced in golden Syrian hamster by intraperitoneal injection of ethanol(1.35 g/kg)and palmitoleic acid(2 mg/kg).The expression of candidate genes,including Actb,Gapdh,Eef2,Ywhaz,Rps18,Hprt1,Tubb,Rpl13a,Nono,and B2m,in hamster pancreas at different time points(1,3,6,9,and 24 h)posttreatment was analyzed using quantitative polymerase chain reaction.The expression stability of these genes was calculated using Best Keeper,Comprehensive Delta CT,Norm Finder,and ge Norm algorithms and Ref Finder software.Results:Our results show that the expression of these reference genes fluctuated during AP,of which Ywhaz and Gapdh were the most stable genes,whereas Tubb,Eef2,and Actb were the least stable genes.Furthermore,these genes were used to normalize the expression of TNF-αmessenger ribonucleic acid in inflamed pancreas.Conclusions:In conclusion,Ywhaz and Gapdh were suitable reference genes for gene expression analysis in AP induced in Syrian hamster.