This paper introduces the synthesis of CoCl2-FeCl3 ternary graphite intercalation compounds (GIC) by fused-salt process, determines and analyses the structure and chemical compositions, and discusses the af-fection of...This paper introduces the synthesis of CoCl2-FeCl3 ternary graphite intercalation compounds (GIC) by fused-salt process, determines and analyses the structure and chemical compositions, and discusses the af-fection of raiv material bending ratio, reaction temperature and reaction time on the yield, cascade structure and distribution of GIC by orthogonal experiment method, and finally decides the optimum experimental condi-tions satisfying different requirements.展开更多
Co^2＋/γ-Al2O3 samples were prepared by incipient wetness impregnation of γ-Al2O3 with different concentration solution of COCl2 and dried at 40 ℃. We measured the positron lifetime spectra of the samples of differ...Co^2＋/γ-Al2O3 samples were prepared by incipient wetness impregnation of γ-Al2O3 with different concentration solution of COCl2 and dried at 40 ℃. We measured the positron lifetime spectra of the samples of different Co2＋ mass fractions （0%-8.24%） heated at different temperatures （100 -500 ℃）. All lifetime spectra were resolved into four components, in which the third and the fourth components were related to the surface state of the micropores and the secondary pores of the γ-Al2O3. The experimental results showed that the Co^2＋ was mainly located in the micropores and the secondary pores near to the exterior of the support. For low Co^2＋ mass fraction samples, when the heating temperature was above 400℃, dispersal was almost finished. When the Co^2＋ mass fraction was above 5.59%, Co^2＋ and Cl were dispersed into the secondary pores in the form of multiple layers.展开更多
Epithelial-specific ETS-1(ESE1), a member of the ETS transcription factor family, is widely expressed in multiple tissues and performs various functions in inflammation. During neuroinflammation, ESE1 promotes neurona...Epithelial-specific ETS-1(ESE1), a member of the ETS transcription factor family, is widely expressed in multiple tissues and performs various functions in inflammation. During neuroinflammation, ESE1 promotes neuronal apoptosis; however, the expression and biological functions of ESE1 remain unclear after cerebral ischemia/reperfusion. We performed in vivo and in vitro experiments to explore the role of ESE1 in cerebral ischemic injury. A modified four vessel occlusion method was used in adult Sprague-Dawley rats. At 6, 12, 24, 48, and 72 hours after model induction, the hippocampus was collected for analysis. Western blot assays and immunohistochemistry showed that the expression of ESE1, phosphorylated p65 and active caspase-3 was significantly up-regulated after ischemia. Double immunofluorescence staining indicated that ESE1 and NeuN were mostly co-located in the hippocampus after ischemia. Furthermore, ESE1 was also co-expressed with active caspase-3. PC12 cells were stimulated with cobalt chloride(CoCl_2) to establish a chemical hypoxia model. After ESE1 knockdown by siRNA for 6 hours, cell viability was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assays. The levels of ESE1, phosphorylated p65 and active caspase-3 were also remarkably increased in PC12 cells after CoCl_2 stimulation. After ESE1 knockdown, PC12 cell viability was increased after hypoxia. siRNA knockdown of ESE1 decreased the level of p-p65 and active caspase-3 after CoCl_2 stimulation. These data reveal that ESE1 levels are elevated in the hippocampus after cerebral ischemia/reperfusion injury. This may play a role in neuronal apoptosis via activation of the nuclear factor-κB pathway.展开更多
A simple and eco-friendly method for the preparation of 1,5-diaryl-3-（arylamino）-lH-pyrrol-2（5H）-ones via the cyclo-condensation reaction of aldehydes, amines and ethyl pyruvate in the presence of silica supported...A simple and eco-friendly method for the preparation of 1,5-diaryl-3-（arylamino）-lH-pyrrol-2（5H）-ones via the cyclo-condensation reaction of aldehydes, amines and ethyl pyruvate in the presence of silica supported ferric chlo- ride （SiO2-FeCl3） as reusable heterogeneous catalyst is described. The present methodology offers several advantages such as excellent yields, simple procedure and short reaction times.展开更多
The perforated breakwater is an environmentally friendly coastal structure,and dissolved oxygen concentration levels are an important index to denote water quality.In this paper,oxygen transport experiments with regul...The perforated breakwater is an environmentally friendly coastal structure,and dissolved oxygen concentration levels are an important index to denote water quality.In this paper,oxygen transport experiments with regular waves through a vertical perforated breakwater were conducted.The oxygen scavenger method was used to reduce the dissolved oxygen concentration of inner water body with the chemicals Na_2SO_3 and Co Cl_2.The dissolved oxygen concentration and wave parameters of 36 experimental scenarios were measured with different perforated arrangements and wave conditions.It was found that the oxygen transfer coefficient through wave surface,K_1a_1,is much lower than the oxygen transport coefficient through the perforated breakwater,K_2a_2.If the effect of K_1a_1 is not considered,the dissolved oxygen concentration computation for inner water body will not be greatly affected.Considering the effect of a permeable area ratio α,relative location parameter of perforations δ and wave period T,the aforementioned data of 30 experimental scenarios,the dimensional analysis and the least squares method were used to derive an equation of K_2a_2(K_2a_2=0.0042α^(0.5)δ^(0.2)T^(-1)).It was validated with 6 other experimental scenarios data,which indicates an approximate agreement.Therefore,this equation can be used to compute the DO concentration caused by the water transport through perforated breakwater.展开更多
文摘This paper introduces the synthesis of CoCl2-FeCl3 ternary graphite intercalation compounds (GIC) by fused-salt process, determines and analyses the structure and chemical compositions, and discusses the af-fection of raiv material bending ratio, reaction temperature and reaction time on the yield, cascade structure and distribution of GIC by orthogonal experiment method, and finally decides the optimum experimental condi-tions satisfying different requirements.
基金Supported by the National Natural Science Foundation of China ( 10675093)
文摘Co^2＋/γ-Al2O3 samples were prepared by incipient wetness impregnation of γ-Al2O3 with different concentration solution of COCl2 and dried at 40 ℃. We measured the positron lifetime spectra of the samples of different Co2＋ mass fractions （0%-8.24%） heated at different temperatures （100 -500 ℃）. All lifetime spectra were resolved into four components, in which the third and the fourth components were related to the surface state of the micropores and the secondary pores of the γ-Al2O3. The experimental results showed that the Co^2＋ was mainly located in the micropores and the secondary pores near to the exterior of the support. For low Co^2＋ mass fraction samples, when the heating temperature was above 400℃, dispersal was almost finished. When the Co^2＋ mass fraction was above 5.59%, Co^2＋ and Cl were dispersed into the secondary pores in the form of multiple layers.
基金supported by the National Natural Science Foundation of China,No.81400963(to HLY)the 333 Project of Jiangsu Province of China,No.BRA2015187(to YZC)the Medical Youth Talent Foundation of Jiangsu Province of China,No.QNRC2016327(to HLY),QNRC2016328(to BLC),and QNRC2016326(to YPL)
文摘Epithelial-specific ETS-1(ESE1), a member of the ETS transcription factor family, is widely expressed in multiple tissues and performs various functions in inflammation. During neuroinflammation, ESE1 promotes neuronal apoptosis; however, the expression and biological functions of ESE1 remain unclear after cerebral ischemia/reperfusion. We performed in vivo and in vitro experiments to explore the role of ESE1 in cerebral ischemic injury. A modified four vessel occlusion method was used in adult Sprague-Dawley rats. At 6, 12, 24, 48, and 72 hours after model induction, the hippocampus was collected for analysis. Western blot assays and immunohistochemistry showed that the expression of ESE1, phosphorylated p65 and active caspase-3 was significantly up-regulated after ischemia. Double immunofluorescence staining indicated that ESE1 and NeuN were mostly co-located in the hippocampus after ischemia. Furthermore, ESE1 was also co-expressed with active caspase-3. PC12 cells were stimulated with cobalt chloride(CoCl_2) to establish a chemical hypoxia model. After ESE1 knockdown by siRNA for 6 hours, cell viability was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assays. The levels of ESE1, phosphorylated p65 and active caspase-3 were also remarkably increased in PC12 cells after CoCl_2 stimulation. After ESE1 knockdown, PC12 cell viability was increased after hypoxia. siRNA knockdown of ESE1 decreased the level of p-p65 and active caspase-3 after CoCl_2 stimulation. These data reveal that ESE1 levels are elevated in the hippocampus after cerebral ischemia/reperfusion injury. This may play a role in neuronal apoptosis via activation of the nuclear factor-κB pathway.
文摘A simple and eco-friendly method for the preparation of 1,5-diaryl-3-（arylamino）-lH-pyrrol-2（5H）-ones via the cyclo-condensation reaction of aldehydes, amines and ethyl pyruvate in the presence of silica supported ferric chlo- ride （SiO2-FeCl3） as reusable heterogeneous catalyst is described. The present methodology offers several advantages such as excellent yields, simple procedure and short reaction times.
基金funded by the National Natural Science Foundation of China (Nos.51579229 and 51009123)
文摘The perforated breakwater is an environmentally friendly coastal structure,and dissolved oxygen concentration levels are an important index to denote water quality.In this paper,oxygen transport experiments with regular waves through a vertical perforated breakwater were conducted.The oxygen scavenger method was used to reduce the dissolved oxygen concentration of inner water body with the chemicals Na_2SO_3 and Co Cl_2.The dissolved oxygen concentration and wave parameters of 36 experimental scenarios were measured with different perforated arrangements and wave conditions.It was found that the oxygen transfer coefficient through wave surface,K_1a_1,is much lower than the oxygen transport coefficient through the perforated breakwater,K_2a_2.If the effect of K_1a_1 is not considered,the dissolved oxygen concentration computation for inner water body will not be greatly affected.Considering the effect of a permeable area ratio α,relative location parameter of perforations δ and wave period T,the aforementioned data of 30 experimental scenarios,the dimensional analysis and the least squares method were used to derive an equation of K_2a_2(K_2a_2=0.0042α^(0.5)δ^(0.2)T^(-1)).It was validated with 6 other experimental scenarios data,which indicates an approximate agreement.Therefore,this equation can be used to compute the DO concentration caused by the water transport through perforated breakwater.
