Three novel L-histidine amide derivatives were synthesized and the corresponding chemical structures were characterized by means of melting point analysis, IR, MS, ^1H NMR as well as ^13C NMR. The coagulation activiti...Three novel L-histidine amide derivatives were synthesized and the corresponding chemical structures were characterized by means of melting point analysis, IR, MS, ^1H NMR as well as ^13C NMR. The coagulation activities of the compounds were evaluated by an MOE（molecular operating environment） docking technique and coagulation test. The results obtained from molecular docking show that the interactions between the compounds and thrombin exhibit procoagulant activity in combination with an improved combinatory effect. Moreover, the results of in vitro coagulation tests show that the L-histidine amide derivatives feature coagulant activities in common coagulation pathways. Compared with the blank control group, the optimal shortening rates of compounds 1-3 were 39.08%（0.5 mmol/L）, 22.94%（1.0 mmol/L） and 15.38%（0.0625 mmol/L）, respectively.展开更多
基金Supported by the National Natural Science Foundation of China（No.21362001）, the Project of the Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, China（No.guizhongzhongkai201104） and the High Level Innovation Team and Outstanding Scholar Project of Guangxi Institutions of Higher Education, China（No.guijiaoren[2014]49）.
文摘Three novel L-histidine amide derivatives were synthesized and the corresponding chemical structures were characterized by means of melting point analysis, IR, MS, ^1H NMR as well as ^13C NMR. The coagulation activities of the compounds were evaluated by an MOE（molecular operating environment） docking technique and coagulation test. The results obtained from molecular docking show that the interactions between the compounds and thrombin exhibit procoagulant activity in combination with an improved combinatory effect. Moreover, the results of in vitro coagulation tests show that the L-histidine amide derivatives feature coagulant activities in common coagulation pathways. Compared with the blank control group, the optimal shortening rates of compounds 1-3 were 39.08%（0.5 mmol/L）, 22.94%（1.0 mmol/L） and 15.38%（0.0625 mmol/L）, respectively.
