The pharmacodynamics of Annexin32, a new Ca2+-dependent phospholipid-binding protein, was studied by measuring coagulation time in rabbits and venous thrombosis in rabbits and rats. Rabbits and rats were given Annexin...The pharmacodynamics of Annexin32, a new Ca2+-dependent phospholipid-binding protein, was studied by measuring coagulation time in rabbits and venous thrombosis in rabbits and rats. Rabbits and rats were given Annexin32 by intravenous administration. Then Kaolin partial thromboplastin time (KPTT), thrombosis in vitro and in vivo were assayed. The results showed that KPTT of rabbits was prolonged (p < 0.01), and the length and weight of thrombus in vitro were reduced (p < 0.01) after administration of Annexin32 at 1 mg/kg. It also inhibited thrombosis in vivo and reduced the weight of venous thrombus significantly in rats (p < 0.01). All these results suggested that Annexin32 possesses the characteristic of antithrombotic effect and fewer side effects on coagulation time.展开更多
In the present study,we aimed to observe the effects of ethanol extract of Oxalis corniculata L.on bleeding time(BT)and coagulation time(CT)and determine its acute toxicity in mice.Firstly,the ethanol extract of O.cor...In the present study,we aimed to observe the effects of ethanol extract of Oxalis corniculata L.on bleeding time(BT)and coagulation time(CT)and determine its acute toxicity in mice.Firstly,the ethanol extract of O.corniculata was administered to 40 mice,which were randomly divided into the normal control group,Yunnan Baiyao control group,low-dose group,and high-dose group.The low-dose(0.6 g/kg)and high-dose(1.2 g/kg)groups received ethanol extract of O.corniculata by gavage,and the BT and CT of the mice were measured by the slide method and tail clipping method,respectively.Secondly,the median lethal dose method was used with 50 mice to observe the physiological state,poisoning reaction,and death of the mice after intragastric administration.Finally,on the 14th day of the experiment,a necropsy was performed to observe any abnormality of the organs.In conclusion,in the coagulation and hemostasis tests,there were no statistical differences between the groups(P>0.05),while there was a significant dose-response relationship,and the BT and CT were significantly shorter than those of the negative control group,which were comparable to the Yunnan Baiyao control group.Moreover,in the acute toxicity test,the median lethal dose of ethanol extract of O.corniculata was 6.0291 g/kg,with a 95%confidence interval of 5.3065–6.7829 mg/kg.展开更多
基金This work was supported by the National High Technology Research and Development Program of China (863 Program).
文摘The pharmacodynamics of Annexin32, a new Ca2+-dependent phospholipid-binding protein, was studied by measuring coagulation time in rabbits and venous thrombosis in rabbits and rats. Rabbits and rats were given Annexin32 by intravenous administration. Then Kaolin partial thromboplastin time (KPTT), thrombosis in vitro and in vivo were assayed. The results showed that KPTT of rabbits was prolonged (p < 0.01), and the length and weight of thrombus in vitro were reduced (p < 0.01) after administration of Annexin32 at 1 mg/kg. It also inhibited thrombosis in vivo and reduced the weight of venous thrombus significantly in rats (p < 0.01). All these results suggested that Annexin32 possesses the characteristic of antithrombotic effect and fewer side effects on coagulation time.
基金Yunnan Province Education Department Scientific Research Fund Project(Grant No.2022J1683)。
文摘In the present study,we aimed to observe the effects of ethanol extract of Oxalis corniculata L.on bleeding time(BT)and coagulation time(CT)and determine its acute toxicity in mice.Firstly,the ethanol extract of O.corniculata was administered to 40 mice,which were randomly divided into the normal control group,Yunnan Baiyao control group,low-dose group,and high-dose group.The low-dose(0.6 g/kg)and high-dose(1.2 g/kg)groups received ethanol extract of O.corniculata by gavage,and the BT and CT of the mice were measured by the slide method and tail clipping method,respectively.Secondly,the median lethal dose method was used with 50 mice to observe the physiological state,poisoning reaction,and death of the mice after intragastric administration.Finally,on the 14th day of the experiment,a necropsy was performed to observe any abnormality of the organs.In conclusion,in the coagulation and hemostasis tests,there were no statistical differences between the groups(P>0.05),while there was a significant dose-response relationship,and the BT and CT were significantly shorter than those of the negative control group,which were comparable to the Yunnan Baiyao control group.Moreover,in the acute toxicity test,the median lethal dose of ethanol extract of O.corniculata was 6.0291 g/kg,with a 95%confidence interval of 5.3065–6.7829 mg/kg.
