Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects.While probiotic cocktails are known to enhance intestinal health,the potential synergistic impact of combining ...Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects.While probiotic cocktails are known to enhance intestinal health,the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored.Therefore,this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the healthpromoting effects.Results A total of 3201-day-old male Arbor Acres broilers were divided into 4 groups,each with 8 replicates of 10 chicks per replicate.Over a period of 42 d,the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin(BC),1,000 g/t probiotic cocktails(PC),or a combination of both BC(37.5 g/t)and PC(1,000 g/t).The results demonstrated that BC+PC exhibited positive synergistic effects,enhancing intestinal morphology,immune function,and barrier function.This was evidenced by increased VH/CD ratio,sIgA levels,and upregulated expression of occludin and claudin-1(P<0.05).16S rRNA analysis indicated that PC potentiated the effects of BC,particularly in the ileum,where BC+PC significantly increased theα-diversity of the ileal microbiota,altered itsβ-diversity,and increased the relative abundance of Flavonifractor(P<0.05),a flavonoid-metabolizing bacterium.Furthermore,Flavonifractor positively correlated with chicken ileum crypt depth(P<0.05).While BC+PC had a limited effect on cecal microbiota structure,the PC group had a very similar microbial composition to BC+PC,suggesting that the effect of PC at the distal end of the gut overshadowed those of BC.Conclusions We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails.Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum,thereby influencing the ileal microbiota structure.This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota.These findings provide compelling evidence for the future development of feed additive combinations.展开更多
目的:研究注射用益气复脉(冻干)Yi Qi Fu Mai Injection(YQFM)(Lyophilized)对大鼠肝微粒体CYP1A2、CYP3A的诱导作用。方法:将wistar大鼠分为生理盐水对照组,苯巴比妥钠诱导组,YQFM组,连续给药7天后处死,制备肝微粒体。采用Cocktail法,...目的:研究注射用益气复脉(冻干)Yi Qi Fu Mai Injection(YQFM)(Lyophilized)对大鼠肝微粒体CYP1A2、CYP3A的诱导作用。方法:将wistar大鼠分为生理盐水对照组,苯巴比妥钠诱导组,YQFM组,连续给药7天后处死,制备肝微粒体。采用Cocktail法,将特异性探针底物非那西丁(CYP1A2)、睾酮(CYP3A)与肝微粒体共孵育,采用高效液相色谱测定孵育所得代谢产物对乙酰氨基酚和6β-羟基睾酮的生成速率,来评价YQFM对CYP1A2、CYP3A的诱导作用。结果:空白对照组、诱导组和YQFM组的对乙酰氨基酚的生成速率分别为(18.04±1.00)、(43.07±2.90)、(27.6±4.5)ng.(mg pro-tein)-1.min-1,6β-羟基睾酮的生成速率分别为(15.79±1.43)、(40.86±3.32)、(32.8±3.67)ng.(mg protein)-1.min-1。结论:YQFM对大鼠肝微粒体CYP1A2、CYP3A有诱导作用。展开更多
基金funded by National Key R&D Program of China(2022YFD1300403,2021YFD1300404)China Agriculture Research System program(CARS-40,CARS-41-G11)Beijing Natural Science Foundation(6222036).
文摘Background Baicalin and probiotic cocktails are promising feed additives with broad application prospects.While probiotic cocktails are known to enhance intestinal health,the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely unexplored.Therefore,this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the healthpromoting effects.Results A total of 3201-day-old male Arbor Acres broilers were divided into 4 groups,each with 8 replicates of 10 chicks per replicate.Over a period of 42 d,the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin(BC),1,000 g/t probiotic cocktails(PC),or a combination of both BC(37.5 g/t)and PC(1,000 g/t).The results demonstrated that BC+PC exhibited positive synergistic effects,enhancing intestinal morphology,immune function,and barrier function.This was evidenced by increased VH/CD ratio,sIgA levels,and upregulated expression of occludin and claudin-1(P<0.05).16S rRNA analysis indicated that PC potentiated the effects of BC,particularly in the ileum,where BC+PC significantly increased theα-diversity of the ileal microbiota,altered itsβ-diversity,and increased the relative abundance of Flavonifractor(P<0.05),a flavonoid-metabolizing bacterium.Furthermore,Flavonifractor positively correlated with chicken ileum crypt depth(P<0.05).While BC+PC had a limited effect on cecal microbiota structure,the PC group had a very similar microbial composition to BC+PC,suggesting that the effect of PC at the distal end of the gut overshadowed those of BC.Conclusions We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic cocktails.Probiotic cocktails enhanced the effects of baicalin and accelerated its metabolism in the ileum,thereby influencing the ileal microbiota structure.This study elucidates the interaction mechanism between probiotic cocktails and plant extract additives within the host microbiota.These findings provide compelling evidence for the future development of feed additive combinations.
文摘目的:研究注射用益气复脉(冻干)Yi Qi Fu Mai Injection(YQFM)(Lyophilized)对大鼠肝微粒体CYP1A2、CYP3A的诱导作用。方法:将wistar大鼠分为生理盐水对照组,苯巴比妥钠诱导组,YQFM组,连续给药7天后处死,制备肝微粒体。采用Cocktail法,将特异性探针底物非那西丁(CYP1A2)、睾酮(CYP3A)与肝微粒体共孵育,采用高效液相色谱测定孵育所得代谢产物对乙酰氨基酚和6β-羟基睾酮的生成速率,来评价YQFM对CYP1A2、CYP3A的诱导作用。结果:空白对照组、诱导组和YQFM组的对乙酰氨基酚的生成速率分别为(18.04±1.00)、(43.07±2.90)、(27.6±4.5)ng.(mg pro-tein)-1.min-1,6β-羟基睾酮的生成速率分别为(15.79±1.43)、(40.86±3.32)、(32.8±3.67)ng.(mg protein)-1.min-1。结论:YQFM对大鼠肝微粒体CYP1A2、CYP3A有诱导作用。
