As an emerging class of non-coding transcripts,circular RNAs(circRNAs)are proved to participate in the complex process of myogenesis in diverse species.A previous study has identified circular RNA EDC3(circEDC3)as a t...As an emerging class of non-coding transcripts,circular RNAs(circRNAs)are proved to participate in the complex process of myogenesis in diverse species.A previous study has identified circular RNA EDC3(circEDC3)as a typical covalently closed circular RNA abundant in chicken skeletal muscle.This study found that circEDC3 is a conservative circular RNA and performed functional analysis to investigate the role of circEDC3 in chicken muscle growth.The results indicated that circEDC3 could inhibit(P<0.05)chicken skeletal muscle satellite cells(SMSCs)proliferation and differentiation but had no significant influence on SMSCs apoptosis.Additionally,bioinformatics analysis showed that circEDC3 had promising coding potential.The open reading frames(ORF)were found in circEDC3 in this study.Furthermore,this study predicted that circEDC3 had internal ribosome entry sites(IRES)and N6-methyladenosine(m6A)motifs in different species,implying that circEDC3 might be translatable.This study revealed that circEDC3 might be a negative regulator in chicken muscle development and suggested it has protein-coding potential in different species.展开更多
Dopamine is a neurotransmitter crucial for movement, mood, drug addiction and many neural degeneration diseases including Parkinson’s disease. Micro electrochemical carbon
基金funded by the Sichuan Science and Technology Program,China(2018JY0488,2016NYZ0050 and 2016NZ0104)。
文摘As an emerging class of non-coding transcripts,circular RNAs(circRNAs)are proved to participate in the complex process of myogenesis in diverse species.A previous study has identified circular RNA EDC3(circEDC3)as a typical covalently closed circular RNA abundant in chicken skeletal muscle.This study found that circEDC3 is a conservative circular RNA and performed functional analysis to investigate the role of circEDC3 in chicken muscle growth.The results indicated that circEDC3 could inhibit(P<0.05)chicken skeletal muscle satellite cells(SMSCs)proliferation and differentiation but had no significant influence on SMSCs apoptosis.Additionally,bioinformatics analysis showed that circEDC3 had promising coding potential.The open reading frames(ORF)were found in circEDC3 in this study.Furthermore,this study predicted that circEDC3 had internal ribosome entry sites(IRES)and N6-methyladenosine(m6A)motifs in different species,implying that circEDC3 might be translatable.This study revealed that circEDC3 might be a negative regulator in chicken muscle development and suggested it has protein-coding potential in different species.
基金Supported by grant from Chinese NSFC "973" program
文摘Dopamine is a neurotransmitter crucial for movement, mood, drug addiction and many neural degeneration diseases including Parkinson’s disease. Micro electrochemical carbon
