The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (la...The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.展开更多
[Objective] The paper was to isolate Colletotrichum sp.from strawberry and to screen effective fungicides.[Method] The indoor toxicity of 7 fungicides to Colletotrichum sp.was measured by growth rate method.The potted...[Objective] The paper was to isolate Colletotrichum sp.from strawberry and to screen effective fungicides.[Method] The indoor toxicity of 7 fungicides to Colletotrichum sp.was measured by growth rate method.The potted control effect of 3 fungicides on Colletotrichum sp.was evaluated.[Result] Fludioxonil exhibited the highest indoor toxicity to Colletotrichum sp.,with the EC50 value of 0.117 mg/L,and the EC50 value of prochloraz and difenoconazole were 0.141 and 0.357 mg/L,respectively.The pot experiment showed that the potted control effect of 20% fludioxonil2 000 times dilution on Colletotrichum sp.was the highest of 92.86%.450 g/L prochloraz EW 4 500 times dilution and 250 g/L difenoconazole EC2 500 times dilution also received high control effects against Colletotrichum sp.,which were 91.68% and 89.29%,respectively.[Conclusion] Fludioxonil,prochloraz and difenoconazole could be used for the control of strawberry anthracnose.展开更多
A new metabolite,named seimatoric acid（1）,representing a new oxobutanoic acid derivative has been isolated from Seimatosporium sp., in addition to four known compounds viz.,2-hydroxymethyl-4β,5α,6β-trihydroxycycl...A new metabolite,named seimatoric acid（1）,representing a new oxobutanoic acid derivative has been isolated from Seimatosporium sp., in addition to four known compounds viz.,2-hydroxymethyl-4β,5α,6β-trihydroxycyclohex-2-enone（2）,（-）-phyllostine（3）,（＋）-epiepoxydon（4） and（＋）-epoxydon monoacetate（5）.Similarly one new benzoic acid derivative,named colletonoic acid（6） was isolated from the ethyl acetate fraction of Colletotrichum sp.The structures of the new compounds were elucidated by detailed ^1 H NMR,^13C NMR,COSY,HMQC.HMBC spectroscopic analysis,and HR-E1-MS.Seimatoric acid（1）was also isolated from another taxonomical unidentified fungal strain 4295 in ourgroup.The structures of the known compounds were elucidated by their spectral data comparison to literature data.Preliminary studies showed that colletonoic acid（6） showed good antibacterial,antifungal,and antialgal activities.展开更多
文摘The oligosaccharide elicitor from the mycelial wall of an endophytic Colletotrichum sp. B501 promoted the production of artemisinin in Artemisia annua L. hairy root culture. When hairy roots of 22-day-old cultures (later growth phase) were exposed to the elicitor (20 mg/L) for 4 d, the maximum content of artemisinin reached 1.15 mg/g, a 64.29% increment over the control. The electron X-ray microanalysis disclosed the rapid accumulation of Ca 2+ in the elicited cortical cells of hairy root. The electronic microscope observation revealed the high electron density area in vacuole of elicited cells. During the first day of elicitation the peroxidase activity of hairy roots was improved sharply. Some cellular morphological changes including cell shrinkage, condensation of cytoplasm and nuclear fragmentation, coincident with the appearance of DNA ladders, were observed after the third day of elicitation. It was suggested that the oligosaccharide elicitor triggered the programmed cell death, which may provide the substance or chemical signal for artemisinin biosynthesis.
基金Supported by Overseas Talents Introduction Project of Shandong Academy of Agricultural Sciences(CXGC2021B04)。
文摘[Objective] The paper was to isolate Colletotrichum sp.from strawberry and to screen effective fungicides.[Method] The indoor toxicity of 7 fungicides to Colletotrichum sp.was measured by growth rate method.The potted control effect of 3 fungicides on Colletotrichum sp.was evaluated.[Result] Fludioxonil exhibited the highest indoor toxicity to Colletotrichum sp.,with the EC50 value of 0.117 mg/L,and the EC50 value of prochloraz and difenoconazole were 0.141 and 0.357 mg/L,respectively.The pot experiment showed that the potted control effect of 20% fludioxonil2 000 times dilution on Colletotrichum sp.was the highest of 92.86%.450 g/L prochloraz EW 4 500 times dilution and 250 g/L difenoconazole EC2 500 times dilution also received high control effects against Colletotrichum sp.,which were 91.68% and 89.29%,respectively.[Conclusion] Fludioxonil,prochloraz and difenoconazole could be used for the control of strawberry anthracnose.
基金BASF AG and the BMBF(Bundesministerium für Bildung und Forschung,No.03F0360A)
文摘A new metabolite,named seimatoric acid（1）,representing a new oxobutanoic acid derivative has been isolated from Seimatosporium sp., in addition to four known compounds viz.,2-hydroxymethyl-4β,5α,6β-trihydroxycyclohex-2-enone（2）,（-）-phyllostine（3）,（＋）-epiepoxydon（4） and（＋）-epoxydon monoacetate（5）.Similarly one new benzoic acid derivative,named colletonoic acid（6） was isolated from the ethyl acetate fraction of Colletotrichum sp.The structures of the new compounds were elucidated by detailed ^1 H NMR,^13C NMR,COSY,HMQC.HMBC spectroscopic analysis,and HR-E1-MS.Seimatoric acid（1）was also isolated from another taxonomical unidentified fungal strain 4295 in ourgroup.The structures of the known compounds were elucidated by their spectral data comparison to literature data.Preliminary studies showed that colletonoic acid（6） showed good antibacterial,antifungal,and antialgal activities.
