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Effects of 105 traditional Chinese medicines on the detection ofβ-agonists in medicine extracts and swine urine based on colloidal gold immunochromatographic assay 被引量:2
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作者 WANG Zi-lin FENG Ke-ying +5 位作者 GE Xiu-feng MAI Jia-cheng WANG Han-chuan LIU Wen-zi ZHANG Jia-hui SHEN Xiang-guang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第6期1626-1635,共10页
Colloidal gold immunochromatographic assay(CGIA)is commonly used for the on-site detection ofβ-agonists that are sometimes used illegally as feed additives in swine diets.However,few studies have evaluated the causes... Colloidal gold immunochromatographic assay(CGIA)is commonly used for the on-site detection ofβ-agonists that are sometimes used illegally as feed additives in swine diets.However,few studies have evaluated the causes of false-positive results that sometimes occur when applying CGIA in agricultural settings.In this study,we investigated if this false-positive phenomenon is related to the addition of certain traditional Chinese medicines(TCMs)to swine feed.We established and verified an extraction method for TCMs,and then applied CGIA to detectβ-agonists in the extracts of 105 TCMs and in the urine of swine dosed with TCMs,respectively.Liquid chromatography-tandem mass spectrometry was used to validate the results of the urine samples tested positive forβ-agonists using CGIA.The results were also verified using TCMs and colloidal gold test strips produced by different manufacturers.The extracts of Citri Reticulatae Pericarpium Viride,Citri Reticulatae Pericarpium,Magnoliae Officinalis Cortex,Chaenomelis Fructus,and Rhodiolae Crenulatae Radix Et Rhizoma were tested positive forβ-agonists.Meanwhile,the addition of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium to swine feed resulted in false-positive results forβ-agonists in swine urine.The results provide a new way to explain false-positive CGIA results and provide valuable information for livestock feeding programs. 展开更多
关键词 colloidal gold immunochromatographic assay false positive traditional Chinese medicine Β-AGONISTS swine urine
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An immunochromatographic assay for rapid and simultaneous detection of levonorgestrel and methylprednisolone in water samples
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作者 Hua-Fang Chang Jun-Qing Wang +1 位作者 Bin Wang An-Ping Deng 《Chinese Chemical Letters》 SCIE CAS CSCD 2013年第10期937-940,共4页
Synthetic contraceptive levonorgestrel (LNG) and glucocorticoid methylprednisolone (MP) residues are eventually discarded to environmental water system and function as environmental hormones, displaying potential ... Synthetic contraceptive levonorgestrel (LNG) and glucocorticoid methylprednisolone (MP) residues are eventually discarded to environmental water system and function as environmental hormones, displaying potential risk to humans and ecosystems, thus there is an urgent need for fast, sensitive and simultaneous detection of these compounds in water samples. In this study, a competitive immunochromatographic assay (ICA) using colloidal gold-labeled polyclonal antibodies as probes for rapid and simultaneous detection of LNG and MP in water samples was developed. The visual detection limits of LNG and MP in water samples were 10 ng/mL. The detection process could be completed within 10 min. There was no cross-reactivity of the ICA with other seven compounds. The strips could be stored at 4 ℃ for 10 weeks without significant loss of activity. The assay is a suitable tool for rapid and semi- quantitative detection of LNG and MP in water samples on site. 展开更多
关键词 immunochromatographic assay kevonorgestrel Methylprednisolone colloidal gold
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