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Improvement of Two-Dimensional Gel Electrophoresis for Study of Corm Formation Related Proteins in vitro from Taro (Colocasia esculenta)
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作者 杜红梅 唐东梅 黄丹枫 《Journal of Shanghai Jiaotong university(Science)》 EI 2005年第S1期14-18,共5页
Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to p... Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to prepare protein samples of taro that was compatible with subsequent analysis using 2D-PAGE. We compared proteins from shoot basal region from 0 d and 2 d after the beginning of tuberization. By this method we got about (2 000) spots and high reproducibility. Additionally some changes of protein expression were found. 展开更多
关键词 colocasia esculenta CORM two-dimensional gel ELECTROPHORESIS sample preparation TUBERIZATION in VITRO
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Preliminary Assessment of Genetic Diversity in Three Taro (Colocasia esculenta L, Schott) Landraces Using Agro-morphological and SSR DNA Characterisation
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作者 Tafadzwanashe Mabhaudhi Albert Thembinkosi Modi 《Journal of Agricultural Science and Technology(B)》 2013年第4期265-271,共7页
Taro (Colocasia esculenta L. Schott) is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula (wild), KwaNgwanase and Umbumbulu, were collected fro... Taro (Colocasia esculenta L. Schott) is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula (wild), KwaNgwanase and Umbumbulu, were collected from two locations in KwaZulu-Natal (KZN), South Africa, and planted at two locations, Pietermaritzburg (KZN) and Roodeplaat, Pretoria. Ago-morphological characterisation of vegetative and corm characteristics were done four months after planting and at harvest, respectively. Sampling for DNA fingerprinting using five SSR primers was done using leaf material four months after planting. Agro-morphological characterisation was useful in showing differences between the wild landrace and the two cultivated landraces, as well as identification of dasheen and eddoe types. SSR primer characterisation showed that despite significant morphological difference, the wild Dumbe Lomfula and Umbumbulu landraces were closely related but different from the KwaNgwanase landrace. Although landraces showed great morphological variation, this did not necessarily imply genetic variation. It is concluded that SSR primers are more useful for characterising taro landraces. 展开更多
关键词 Agro-morphology characterisation DNA LANDRACES SSR primers taro colocasia esculenta L. Schott) geneticdiversity.
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Evaluation of the Value Nutritious Leaves Five Varieties Taro (Colocasia esculenta) Cultivated in Burkina Faso
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作者 Boampoundi Hélène Ouoba Amana Metuor Dabire +4 位作者 Sidnooma Véronique Zongo Hemayoro Sama Samson Guenne Renan Ernest Traore Jacques Simpore 《Food and Nutrition Sciences》 CAS 2022年第10期835-841,共7页
Taro is a perennial herbaceous plant whose large leaves are mainly used as vegetables in human food in several tropical countries. However, young taro leaves are not eaten very much in Burkina Faso unlike other countr... Taro is a perennial herbaceous plant whose large leaves are mainly used as vegetables in human food in several tropical countries. However, young taro leaves are not eaten very much in Burkina Faso unlike other countries which have made them a staple diet. In the present work, we collected leaves of taro varieties cultivated in the provinces of Comoe and Kenedougou. Our study aimed to determine the biochemical composition of these leaves in order to detect their nutritional quality. For this purpose, we first determined the total sugars in our different samples;then quantify the proteins and finally assay the lipids contained in the leaves of the different varieties of taro harvested. Analysis of the organic constituents gave the following results: proteins (186.29 to 265.23 μg EQ/100mg fresh leaves), fats (0.28% to 1.90%), carbohydrates (183.03 to 238.57 μg EG/100mg fresh leaves). The highest energy value was obtained with the variety BF/CO/06 (1728.71 kcal/kg) and the lowest with BF/CO/04 (272.15 kcal/kg). This study allowed us to conclude that the taro leaves (Colocasia esculenta) studied are of nutritional interest with regard to their biochemical composition. 展开更多
关键词 colocasia esculenta Nutritional Value LEAVES
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Comparison of the Effectiveness of Different Traditional Soaking Processes on the in Vitro Digestibility of Taro(Colocasia esculenta L.SCHOTT)Flour
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作者 Imar Djibrine Soudy Laurent Alves de Oliveira +7 位作者 Nicolas Limas Nzouzi Mamadou Godefroy Izzedine Abdelaziz Arada Ousmane Dahab Atteib Djidda Alhadj Alfaroukh Oumar Idriss Bruno Eto Denis Grancher 《Food and Nutrition Sciences》 2014年第3期258-263,共6页
A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of ta... A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of taro and facilitate cooking. The aim of this study was to assess the effect of traditional soaking on the in vitro digestibility of taro flour using or not using an α-amylase enzyme. The digestion without the enzyme has shown that the soaking processes improve the digestibility of taro flour (from 39.30% for the control sample to 75.11% (after tamarind infusion) and 78.67% (treatment with water) after 24 hours of soaking). Soaking over a 6-hour period and preferentially in tamarind infusion or in corn solution obtains highly digestible flour (around 95% of digestibility rate after 3 hours of enzymatic digestion). 展开更多
关键词 colocasia esculenta L.SCHOTT SOAKING CORN Zea mays L. Tamarind Tamarindus indica L. In Vitro Di-gestibility AMYLASE
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芋(Colocasia esculenta)的民族植物学 被引量:1
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作者 李庆典 杨永平 +1 位作者 李颖 周清明 《植物资源与环境学报》 CAS CSCD 2005年第1期44-48,共5页
应用民族植物学的基本原理和方法,选择中国云南和山东为试点,兼顾其他省区,开展芋〔Colocasiaesculenta(L. )Schott〕的民族植物学研究。结果表明:在云南传统栽种芋的菜园和农地被高附加值的经济作物所代替,芋在不同民族家庭中的地位也... 应用民族植物学的基本原理和方法,选择中国云南和山东为试点,兼顾其他省区,开展芋〔Colocasiaesculenta(L. )Schott〕的民族植物学研究。结果表明:在云南传统栽种芋的菜园和农地被高附加值的经济作物所代替,芋在不同民族家庭中的地位也从传统作为主食变成蔬菜或杂粮;在山东已形成芋的产业化、标准化生产的格局,芋在汉族农家经济中的地位得到提升。在云南分布有芋的野生近缘种、半栽培种、栽培品种,种质资源丰富;在山东未发现芋的野生类型,以旱芋类型的多子芋栽培品种为主。由于经济的发展和主流文化的影响,民间对芋的植物崇拜及植物崇拜文化丢失的速度大大加剧。在古朴的传统食芋文化中,蕴含着丰富的关于芋植物资源利用和保护的传统知识和朴素的科学内涵,需要进行深入的挖掘和探讨。 展开更多
关键词 民族植物学 食芋文化 种质资源 植物崇拜 资源保护
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芋[Colocasiaesculenta(L.) Schott]的倍性水平及基因组特征解析 被引量:2
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作者 殷剑美 王立 +4 位作者 蒋璐 韩晓勇 郭文琦 李春宏 张培通 《江苏农业学报》 CSCD 北大核心 2019年第6期1284-1291,共8页
采用流式细胞术,对2个芋[Colocasiaesculenta(L.)Schott]品种苏芋1号和香蕉芋的倍性水平和DNA含量进行了测定,2倍基因组DNA含量分别为7.71 pg和5.25 pg,倍性分别为三倍体和二倍体。同时通过HiSeq 2500测序,分别获得240.53 Gb和87.69 Gb... 采用流式细胞术,对2个芋[Colocasiaesculenta(L.)Schott]品种苏芋1号和香蕉芋的倍性水平和DNA含量进行了测定,2倍基因组DNA含量分别为7.71 pg和5.25 pg,倍性分别为三倍体和二倍体。同时通过HiSeq 2500测序,分别获得240.53 Gb和87.69 Gb数据,芋基因组大小为2.08~2.38 Gb,GC含量为42.57%~42.75%,杂合度为0.52%~2.29%。根据苏芋1号的基因组数据,组装得到基因组序列为2.27 Gb,Contig N50为359 bp;共注释获得24302个基因,99.43%的基因可注释到6个公共数据库;筛选出928987个SSR位点。 展开更多
关键词 流式细胞术 基因组测序 基因功能注释 倍型水平 基因组特征
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Transcriptome analysis of purple pigment formation in Colocasia esculenta 被引量:3
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作者 FANGLIAN HE WEIQING DONG +5 位作者 SHAOLONG WEI ZUYANG QIU JINGLI HUANG HUIPING JIANG SHIYU HUANG LILI LIU 《BIOCELL》 SCIE 2021年第3期785-796,共12页
Taro(Colocasia esculenta(L.)Schott)is an important crop in Africa,Southeast Asia,and subtropics and is used as a food and medicine.The purple color pigmentation is an appealing character in taro.We sampled taro corms ... Taro(Colocasia esculenta(L.)Schott)is an important crop in Africa,Southeast Asia,and subtropics and is used as a food and medicine.The purple color pigmentation is an appealing character in taro.We sampled taro corms of the cultivar‘Lipu Taro’at four developmental stages,including LPYS1(without purple pigment,50 days of development(DOD)),LPYS2(very few purple pigments,75 DOD),LPYS3(moderate purple pigments,115 DOD)and LPYS4(high purple pigments,205 DOD).The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing.Through RNA-Seq,6453 differentially expressed transcripts(DETs)were identified between purple and non-purple pigmented samples.We identified 41 and 12 flavonoid and anthocyanin related DETs transcripts,respectively.These DETs were upregulated at LPYS2,LPYS3,and LPYS4 as compared to LPYS1,indicating their positive contribution to the color formation in taro.Moreover,we identified several DETs encoding for transcription factors,including MYB and bHLH,known to be major regulators of structural genes involved in the flavonoid-anthocyanin pathway.Finally,we reported several plant hormones(ethylene,auxin,gibberellin,jasmonic acid,and cytokinin)related DETs,which are predicted to play important roles in the corm coloration.Different regulation of transcripts representing the flavonoid-anthocyanin biosynthesis pathway,plant hormone transduction pathway,and transcription factors may have key roles in purple pigmentation in taro.Our findings will facilitate future research on improving the quality and appeal of taro. 展开更多
关键词 ANTHOCYANIN colocasia esculenta Color formation Plant hormones Gene expression Transcription factors
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Characterization of a Highly Potent Insecticidal Lectin from <i>Colocasia esculenta</i>Tuber and Cloning of Its Coding Sequence 被引量:1
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作者 Ayan Das Amit Roy +1 位作者 Daniel Hess Sampa Das 《American Journal of Plant Sciences》 2013年第2期408-416,共9页
Hemipteran insects are the most devastating pest for different crops of high economic value. Colocasia esculenta tuber agglutinin (CEA), a mannose binding monocot lectin from araceae family was previously reported by ... Hemipteran insects are the most devastating pest for different crops of high economic value. Colocasia esculenta tuber agglutinin (CEA), a mannose binding monocot lectin from araceae family was previously reported by the present group to be effective against some members of this class of pests. In the present study, efficacy of this potent lectin has been extended to cotton aphid (Aphis gossypii) which is becoming a highly damaging pest of cotton in recent days. Because, like other aphids, A. gossypii not only extracts the phloem fluid but also transmit disease causing viruses and add to the high degree of yield loss. Efficacy of the lectin on cotton aphid as well as other hemipteran insects prompted us further to clone the protein coding gene. Very little sequence information of this gene was available in the database. Hence, attempt had been made to study the protein through liquid chromatography-tandem mass spectrometry (LC-MS/MS) to have the detailed peptide information. On the basis of the peptide homology information obtained from LC-MS/MS the complete coding sequence of CEA was determined. The coding sequence corresponding to CEA was cloned further using primers designed on the basis of above information and genome walk technology for its potential utilisation in insect management programme. 展开更多
关键词 colocasia esculenta TUBER AGGLUTININ (CEA) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Genome Walk Hemipteran INSECT INSECT Bioassay
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Synthesis of silver nanoparticle with Colocasia esculenta(L.) stem and its larvicidal activity against Culex quinquefasciatus and Chironomus sp
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作者 Arghadip Mondal Amita Hajra +2 位作者 Wasim Akram Shaikh Sukalyan Chakraborty Naba Kumar Mondal 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2019年第12期510-517,共8页
Objective:To synthesize silver nanoparticles with Colocasia esculenta as a reducing agent and to evaluate their effect against Culex quinquefasciatus and Chironomus sp.Methods:The aqueous extract of Colocasia esculent... Objective:To synthesize silver nanoparticles with Colocasia esculenta as a reducing agent and to evaluate their effect against Culex quinquefasciatus and Chironomus sp.Methods:The aqueous extract of Colocasia esculenta stem was used for nanosynthesis.The synthesized nanoparticles were characterized by UV-Vis spectrophotometry,Fourier-transform infrared spectroscopy,scanning electron microscope,transmission electron microscopy,energy-dispersive X-ray spectroscopy,X-ray diffraction and Zeta potential studies.The toxicity of Colocasia esculenta stem extract and the synthesized silver nanoparticles was evaluated against the larval stages of target human filarial vector Culex quinquefasciatus and non-target Chironomus sp.Results:Scanning electron microscopy and transmission electron microscopy studies revealed almost spherical shape of the synthesized silver nanoparticles with size ranging from 13-50 nm.After 24 hours of exposure,the LC50 and LC90 of the plant extract against 4th instars larvae of Culex quinquefasciatus were 745.56 mg/L and 1258.28 mg/L,respectively,which were higher than those of synthesized silver nanoparticles(5.17 mg/L and 17.32 mg/L after 24 h;1.58 mg/L and 13.01 mg/L after 48 h).In addition,the LC50 and LC90 of silver nanoparticles against Chironomus sp.were 9.71 mg/L and 23.15 mg/L after 24 h as well as 2.38 mg/L and 19.49 mg/L after 48 h,respectively.Conclusions:The aqueous stem extract of Colocasia esculenta is a good agent for synthesis of silver nanoparticles,which are almost spherical with size less than 30 nm.The synthesized nanoparticles show good larvicidal activity without any harmful effect on non-target species. 展开更多
关键词 Silver nanoparticles colocasia esculenta LARVICIDAL CULEX quinquefasciatus MORTALITY CHIRONOMUS SP
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<i>In Vitro</i>Organogenesis of <i>Colocasia esculenta</i>cv. <i>Antiquorum</i>L.
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作者 Md. Jahangir Hossain 《American Journal of Plant Sciences》 2012年第6期709-713,共5页
In vitro organogenesis of an upland species of Colocasia esculenta cv. antiquorum L. was examined in relation to different explants like meristem and parenchymatous storage tissues with or without anthocyanin layer, f... In vitro organogenesis of an upland species of Colocasia esculenta cv. antiquorum L. was examined in relation to different explants like meristem and parenchymatous storage tissues with or without anthocyanin layer, four levels of each of Kn, 2,4-D, NAA and BAP and four incubation environments such as: 1) 16 h 3 Kl light intensity + 24°C ± 2°C;2) 24 h dark + 24°C ± 2°C;3) 24 h dark + 30°C ± 3°C and 4) 12 h diffuse light + 30°C ± 3°C. Only meristems showed proliferation with various degree of intensity both at 16 h 3 Kl light + 24°C ± 2°C and 24 h dark + 24°C ± 2°C conditions and poor response with different levels of Kn + NAA either in light or in the dark. Cultures with NAA + BAP were proliferated very quickly with very high degree of intensity. The cultures under dark did not proliferate for 20 days which upon transfer to light showed high degree of proliferation. Cultures with NAA + BAP formed calluses more pronouncedly at dark than that occurred in the light. Parenchymatous tissues with or without anthocyanin did not proliferate but the tissues with anthocyanin lost pigmentation after 25 - 30 days and turned to grey colour after 50 days while tissues without anthocyanin turned to green colour with shinny pimples indicating that protocorm may be developed. No culture under high temperature environment (30°C ± 3°C) neither survived nor proliferated. The meristems in culture were died within 15 - 20 days while others within 25-30 days. In conclusion, a combination of NAA (0.5 - 3.0 mg/l) and BAP (0.5 - 2.0 mg/l) and an incubation photoperiod of 16 h coupled with temperature of 24°C ± 2°C were found most suitable for in vitro culture of Colocasia esculenta cv. antiquorum L. 展开更多
关键词 ORGANOGENESIS Auxin Cytokinin MERISTEM Parenchymatous Storage Tissue In Vitro colocasia esculenta cv. Antiquorum L
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Effect of Different Levels of Nitrogen on Yield of Colocasia (<i>Colocasia esculenta</i>) at District Malakand Dargai
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作者 Rafiq Ahmad Dost Muhammad +4 位作者 Maria Mussarat Shah Fahad Shahid Ullah Taimur Ahmad Sara Wahab 《Open Journal of Soil Science》 2018年第2期87-98,共12页
A field experiment was conducted to evaluate the response of colocasia (Colocasia esculenta) to different levels of 0, 60, 90, 120 and 150 kg N ha-1 under farmer’s field condition at Garhi Usmani Khel, District Malak... A field experiment was conducted to evaluate the response of colocasia (Colocasia esculenta) to different levels of 0, 60, 90, 120 and 150 kg N ha-1 under farmer’s field condition at Garhi Usmani Khel, District Malakand Dargai during 2013. The experiment was laid out in Randomized Complete Block design with three replications and treatment plot size of 2.74 × 2.43 m2. All levels of N in the form of urea along with uniform basal doze of 90 kg P2O5 ha-1 as Triple Super Phosphate (TSPPP) were applied to soil at time of seed bed followed by thorough mixing. Seeds of colocasia c.v. local variety were sown in these plots with row spacing of 30 cm and plant to plant distance of 12 cm in February, 2013. The results showed that application of N produced significantly higher colocasia tuber yield, number of tubers plant-1, 1000-tubers weight and size of tubers (mean length and diameter) over control but the differences among levels of N were nonsignificant. However, some parameters like tuber yield was maximum at 60 kg N ha-1 and tuber size especially the length of colocasia tuber was maximum at 150 kg N ha-1 suggesting that the response of each parameter was different to N levels. Based on maximum relative yield (100%) and increase over control (46.1%) still at lower N levels of 60 kg N ha-1, this level seems to be appropriate level for colocasia under the prevailing soil and climatic conditions. 展开更多
关键词 colocasia esculenta NITROGEN TUBER YIELD Agronomic Character Pakistan
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Molecular Detection of Phytophthora colocasiae of Taro Leaf Blight Based on PCR
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作者 HUANG Weihua YAN Meixin 《Journal of Landscape Research》 2020年第1期33-35,38,共4页
The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmiss... The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmission and spread of the causal organism of taro leaf blight in taro planting regions.The samples were used to extract total DNA and to be detected by PCR with P.colocasiae specific primer pairs PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.Distinct fragments of about 200 bp and 240 bp were amplified by PCR using primers PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.The analysis of the nucleotide sequence of the PCR products were found to be 99% identical to sequence of RAS-related protein (Ypt1) and phospho-ribosylanthranilate isomerase (TRP1) in P.colocasiae,respectively.It is concluded that rapid and sensitive developed PCR assay for detection of P.colocasiae could be used in routine diagnosis and aid in management practices to mitigate taro leaf blight. 展开更多
关键词 colocasia esculenta(L.)Schott taro leaf blight Phytophthora colocasiae Molecular detection
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不同红芽芋地方品种新鲜子芋自然富集元素能力的鉴定与评价
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作者 陈涵 肖敏 +4 位作者 程建峰 邓文峰 邓锦明 胡润雨 黄英金 《江西农业大学学报》 CAS CSCD 北大核心 2024年第4期867-883,共17页
【目的】红芽芋为典型的多子芋类型,品质风味居芋类之首,是老幼皆宜和药食兼优的滋补佳品。阐明红芽芋子芋自然富集元素能力的基因型间差异,筛选出高富集有益元素且低富集有害元素的地方品种,为高产优质营养安全红芽芋地方品种的遗传改... 【目的】红芽芋为典型的多子芋类型,品质风味居芋类之首,是老幼皆宜和药食兼优的滋补佳品。阐明红芽芋子芋自然富集元素能力的基因型间差异,筛选出高富集有益元素且低富集有害元素的地方品种,为高产优质营养安全红芽芋地方品种的遗传改良和品种选择提供理论依据及实践指导。【方法】以21个在不同原产地广泛种植的红芽芋地方品种为材料,统一种植在同一块大田,采用电感耦合等离子体质谱法分析其新鲜子芋中有益元素[镁(Mg)、铁(Fe)、锌(Zn)、硒(Se)]和有害元素[镉(Cd)、铬(Cr)、铅(Pb)、汞(Hg)和砷(As)]的含量及累积量,并利用模糊隶属函数法、熵权优劣解距离法和模糊综合评判法对其自然富集元素能力进行综合鉴定与评价。【结果】不同红芽芋地方品种的新鲜子芋产量存在巨大差异,最高的HY09(浙江嘉兴)为最低的HY10(云南昆明)的4.5倍。不同红芽芋地方品种新鲜子芋自然富集元素能力(含量及累积量)存在明显差异,无论是同一红芽芋地方品种新鲜子芋的不同有益和有害元素含量及累积量间还是不同红芽芋地方品种新鲜子芋的同一有益和有害元素含量及累积量间均存在显著或极显著差异。有益元素中,以镁含量及累积量最高,其次为铁和锌,硒极低;但品种间的锌含量及累积量的差异最大,铁和硒次之,镁差异最小。有害元素中,以镉含量及累积量最高,汞最低,铬的品种间差异最大,砷的品种间差异最小。红芽芋地方品种对Cd的富集能力较强,对Mg的富集中等,对其他元素的富集能力极小。红芽芋新鲜子芋中的Se与Fe、Cr、Hg和As,Fe与Zn、Cr、Hg和As,Zn与Cr、Hg和As,Mg与Cd,Cr与As和Hg及Hg与As间的含量及累积量间均呈极显著正相关。【结论】综合分析显示,不同红芽芋新鲜子芋天然富集元素能力的可分为高、中和低三类,高富集能力的品种是HY14(湖南永州),中等富集能力的品种是HY04(江苏无锡)和HY20(江西赣州),其他均为低富集能力品种。 展开更多
关键词 红芽芋 富集 元素 基因型差异 鉴定与评价
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多子芋种质资源主要农艺及品质性状鉴定评价与优异种质筛选
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作者 黄新芳 孙亚林 +17 位作者 杨英楠 柯卫东 彭静 董红霞 刘玉平 李峰 李双梅 朱红莲 刘义满 黄来春 李明华 周凯 匡晶 刘正位 王芸 钟兰 季群 王直新 《中国蔬菜》 北大核心 2024年第7期92-98,共7页
从国家水生蔬菜种质资源圃(武汉)中选择62份有代表性的不同类型多子芋种质资源,对其主要农艺性状及品质性状进行鉴定评价和优异种质资源筛选。结果表明,白芽多子芋的株高、生育期、单个子孙芋质量、干物质含量、淀粉含量均低于红芽多子... 从国家水生蔬菜种质资源圃(武汉)中选择62份有代表性的不同类型多子芋种质资源,对其主要农艺性状及品质性状进行鉴定评价和优异种质资源筛选。结果表明,白芽多子芋的株高、生育期、单个子孙芋质量、干物质含量、淀粉含量均低于红芽多子芋,而叶面积、分株数量、母芋质量、子孙芋质量、球茎总质量、商品芋质量、子孙芋数量均高于红芽多子芋。生育期与株高、母芋质量、单个子孙芋质量呈极显著正相关,与分株数量呈极显著负相关,与叶面积、干物质含量、淀粉含量呈显著正相关,与子孙芋数量呈显著负相关。株高、叶面积、子孙芋质量、球茎总质量、商品芋质量间两两呈极显著正相关。干物质含量和淀粉含量与子孙芋质量、球茎总质量、商品芋质量呈极显著负相关,与单个子孙芋质量呈显著负相关。在此基础上,筛选出优异种质资源10份,其中包含3份特异种质资源。 展开更多
关键词 多子芋 种质资源 农艺性状 品质性状 鉴定评价 筛选
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芋可溶性淀粉合成酶CeSS基因家族的克隆和表达分析 被引量:1
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作者 王立 殷剑美 +4 位作者 韩晓勇 蒋璐 郭文琦 金林 张培通 《江苏农业学报》 CSCD 北大核心 2023年第4期939-946,共8页
为探索芋淀粉合成机理,本研究从靖江香沙芋中克隆获得了3个芋可溶性淀粉合酶(CeSS)家族基因。3个基因CeSSI、CeSSII、CeSSIII碱基序列全长分别为1932 bp、2409 bp、3606 bp,编码形成3个亲水性蛋白质。系统进化分析结果显示,3个蛋白质Ce... 为探索芋淀粉合成机理,本研究从靖江香沙芋中克隆获得了3个芋可溶性淀粉合酶(CeSS)家族基因。3个基因CeSSI、CeSSII、CeSSIII碱基序列全长分别为1932 bp、2409 bp、3606 bp,编码形成3个亲水性蛋白质。系统进化分析结果显示,3个蛋白质CeSSI、CeSSII、CeSSIII与海枣、芦笋、药用稻等单子叶植物的亲缘关系较近。表达分析结果表明,这3个CeSS基因广泛存在于芋的叶片、叶柄、母芋和子芋中。CeSSI和CeSSII的表达水平随芋球茎的发育而显著增加,在种植150 d达到峰值,而CeSSIII在成熟后期(种植180 d)的芋球茎中表达量较高。 展开更多
关键词 可溶性淀粉合成酶 基因克隆 表达分析
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Genetic Diversity of Taro Landraces from Côte d’Ivoire Based on Qualitative Traits of Leaves
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作者 Jean-Michel Niambet Koffi Kevin Kouamé Koffi +1 位作者 Severin Beket Bonny Arsène Irié Zoro Bi 《Agricultural Sciences》 2021年第12期1433-1446,共14页
Taro is an important crop species in C<span style="white-space:nowrap;">&#244;</span>te d’Ivoire. It is cultivated for its tubers and leaves. But its knowledge and genetic diversity and diff... Taro is an important crop species in C<span style="white-space:nowrap;">&#244;</span>te d’Ivoire. It is cultivated for its tubers and leaves. But its knowledge and genetic diversity and differentiation are very weakly documented. Several morphological types are found in rural area, but their identification is not very clear, and their agronomic potentiality is underexploited. In this context we initiated a survey and collected 213 accessions from 14 growing regions of C<span style="white-space:nowrap;">&#244;</span>te d’Ivoire. The diversity was evaluated based on seven qualitative traits of leaves (Shape of the base of the leaf, Predominant position (shape) of the leaf blade surface, Margin of the leaf blade, Leaf blade variegation, Profile of the petiolar junction, Shape of the leaf sheath, Shape of the appendix) during an experimentation conducted in rural area. The objective of this study was to characterize the collection of taros collected in different geographical zones of C<span style="white-space:nowrap;">&#244;</span>te d’Ivoire and identify the genera cultivated. Results of our study indicated that excepted margin of the leaf blade all traits are very discriminant. Several variants were observed for each of traits. According to observations and statistics analysis accessions were separated into two main groups. The characteristics of these groups indicate that taro cultivated in C<span style="white-space:nowrap;">&#244;</span>te d’Ivoire could belong to two genera: <em>Xanthosoma</em> and <em>Colocasia</em>. 展开更多
关键词 Côte d’Ivoire colocasia Diversity taro Xanthosoma
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江西铅山红芽芋叶绿体基因组特征及系统发育分析 被引量:1
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作者 尹明华 余锾媛 +1 位作者 肖心怡 王玉婷 《生物技术通报》 CAS CSCD 北大核心 2023年第6期233-247,共15页
为分析江西铅山红芽芋叶绿体基因组的结构组成情况,判定其在芋属中的进化位置及与同芋属叶绿体基因组的区别,为芋属物种鉴定、遗传多样性分析和资源保护提供相关依据。使用Illumina NovaSeq 6000测序平台对江西铅山红芽芋叶绿体基因组... 为分析江西铅山红芽芋叶绿体基因组的结构组成情况,判定其在芋属中的进化位置及与同芋属叶绿体基因组的区别,为芋属物种鉴定、遗传多样性分析和资源保护提供相关依据。使用Illumina NovaSeq 6000测序平台对江西铅山红芽芋叶绿体基因组进行测序,通过生物信息学分析方法进行序列组装、注释和特征分析,并利用Geneious、MISA、MAFF、FASTTREE、CUSP、Chips和Codon W等生物信息学软件对其基因组结构和数目、密码子偏好性、序列重复、SSR位点和系统发育进行分析。结果表明,江西铅山红芽芋叶绿体基因组大小为16 2544 bp,呈现四分体结构。共包含130个基因,其中84个编码蛋白质的基因,37个t RNA基因,8个核糖体rRNA基因,1个假基因。通过密码子偏好性分析,平均有效密码子数为45.60,ENC值小于45的基因39个,说明其密码子偏好性强。通过SSR分析,检测到101个SSR位点,其中单核苷酸重复最多(约占83.17%),且单核苷酸以A/T型为主。与近缘种比较,发现其叶绿体基因组序列高度保守,尤其蛋白质编码序列相似度极高。此外,系统发育分析发现江西铅山红芽芋与芋属、岩芋属聚为一支。本研究得到了江西铅山红芽芋的叶绿体基因组基本情况与系统发育位置,为江西铅山红芽芋的物种辨别、天然种群遗传多样性与功能基因组学提供前期研究铺垫。 展开更多
关键词 江西铅山红芽芋 叶绿体基因组特征 系统发育分析 SSR分析 IR边界分析 密码子偏好性 最优密码子
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红芽芋及荔浦芋叶绿体基因组测序及比较分析 被引量:2
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作者 贾芯碧 潘饶 +7 位作者 肖遥 罗莎 单楠 孙静宇 汪生林 周庆红 黄英金 朱强龙 《热带作物学报》 CSCD 北大核心 2023年第5期880-893,共14页
为明确红芽芋、荔浦芋叶绿体基因组的基本特征及其差异,本研究以江西铅山红芽芋和广西荔浦芋为研究对象,采用改良CTAB法提取2种芋新鲜叶片基因组DNA,利用高通量测序技术获得2种芋全长叶绿体基因组序列,并进行SSR检测、序列比对、遗传多... 为明确红芽芋、荔浦芋叶绿体基因组的基本特征及其差异,本研究以江西铅山红芽芋和广西荔浦芋为研究对象,采用改良CTAB法提取2种芋新鲜叶片基因组DNA,利用高通量测序技术获得2种芋全长叶绿体基因组序列,并进行SSR检测、序列比对、遗传多样性滑窗分析和系统发育树构建分析。结果表明,红芽芋和荔浦芋的叶绿体基因组全长分别为162478 bp和162453 bp,均呈现典型的环状双链四分体结构,二者均注释到131个基因,包括蛋白编码基因86个,tRNA基因37个,rRNA基因8个,其中具有2个拷贝的基因18个,具有内含子的基因23个。简单重复序列(SSR)位点分析发现,红芽芋和荔浦芋基因组序列分别含有130和124个SSR,二者SSR序列中A/T碱基含量分别占63.08%和61.29%,基于其SSR位点分析开发出15条具有多态性的SSR引物。红芽芋和荔浦芋叶绿体基因组共检测到236个SNP位点,26.7%的SNP位点发生在编码区,其中有25个基因发生错位突变,这些基因的变异可能促进了2种芋之间的性状变异。与天南星亚科已公布的13个属代表植物叶绿体基因组进行比较,其基因结构、种类和数量都比较保守,trnS-trnG和ndhF-rpl32分别是SSC区和LSC区的最高变异位点。最大似然法构建的系统发育树显示芋属与皂七属亲缘关系最为相近,与斑龙芋属亲缘关系较远。本研究结果将为我国特色芋种质资源开发利用、遗传多样性评价和系统发育分析奠定理论基础。 展开更多
关键词 红芽芋 荔浦芋 叶绿体基因组 SSR SNP 系统发育分析
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江西槟榔芋疫病病原菌鉴定及室内药剂筛选 被引量:2
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作者 邹芬 何烈干 +2 位作者 李湘民 黄瑞荣 马辉刚 《植物保护》 CAS CSCD 北大核心 2023年第1期311-316,共6页
为明确江西省九江市槟榔芋疫病的病原菌并有针对性地对该病害进行防治,本研究采集具有典型芋疫病症状的芋叶片和叶柄对病原菌进行分离纯化,结合形态学观察、致病性测定及基于ITS、Ypt1和β-tubulin基因联合系统发育分析对该病原菌进行鉴... 为明确江西省九江市槟榔芋疫病的病原菌并有针对性地对该病害进行防治,本研究采集具有典型芋疫病症状的芋叶片和叶柄对病原菌进行分离纯化,结合形态学观察、致病性测定及基于ITS、Ypt1和β-tubulin基因联合系统发育分析对该病原菌进行鉴定,并测定了其对7种杀菌剂的敏感性。根据病原菌菌落、卵孢子形态等特征初步鉴定为芋疫霉Phytophthora colocasiae,基于多基因联合系统发育分析结果与形态学结果一致,确定该病原菌为芋疫霉。室内药剂筛选结果表明,23.4%双炔酰菌胺SC对菌丝生长抑制效果最好,EC_(50)为0.006μg/mL;96.8%烯酰吗啉TC、0.3%丁子香酚SL、20%氟吗啉WP、500 g/L氟啶胺SC、97%霜脲氰TC对菌丝生长的抑制效果也较好,EC_(50)介于0.094~1.272μg/mL之间,研究结果可为槟榔芋疫病的化学防治提供参考。 展开更多
关键词 槟榔芋疫病 芋疫霉 病原菌鉴定 药剂筛选
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芋扩展蛋白基因家族的全基因组鉴定及其在球茎膨大中的表达分析
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作者 李必聪 李慧英 +4 位作者 肖遥 罗莎 周庆红 黄英金 朱强龙 《浙江农业学报》 CSCD 北大核心 2023年第7期1604-1616,共13页
扩展蛋白是一类具有促进细胞壁伸长和增加细胞壁柔韧性作用的蛋白。本研究基于芋全基因组范围鉴定扩展蛋白基因,并分析该家族基因结构特征、系统发育及其在芋球茎膨大中的表达模式。结果表明,芋全基因组包含31个扩展蛋白基因,分布在芋8... 扩展蛋白是一类具有促进细胞壁伸长和增加细胞壁柔韧性作用的蛋白。本研究基于芋全基因组范围鉴定扩展蛋白基因,并分析该家族基因结构特征、系统发育及其在芋球茎膨大中的表达模式。结果表明,芋全基因组包含31个扩展蛋白基因,分布在芋8条染色体及2个重叠群上。在芋和拟南芥、水稻、马铃薯等4个物种中的扩展蛋白基因进行系统发育树分析中,所有的扩展蛋白基因划分到4个亚家族中,其中EXPA有23个,EXPB有5个,EXLA有1个,EXLB有2个。共线性分析表明,芋和拟南芥之间存在7对共线性扩展蛋白基因。基因表达分析发现,芋扩展蛋白基因家族不同成员在不同组织中有不同的表达模式,其中CeEXPA 2、CeEXPA 11、CeEXPA 13、CeEXPA 23和CeEXLA 1等5个基因在球茎膨大过程中高表达且差异显著,表明它们可能对芋球茎的膨大发挥了重要的调控作用。本研究结果将有助于了解芋扩展蛋白基因家族的特征及其在球茎膨大中的分子功能,为揭示芋球茎生长发育的分子调控机理提供理论依据。 展开更多
关键词 扩展蛋白基因家族 球茎膨大 基因表达分析
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