Taro （Colocasia esculenta L. Schott） is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula （wild）, KwaNgwanase and Umbumbulu, were collected fro...Taro （Colocasia esculenta L. Schott） is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula （wild）, KwaNgwanase and Umbumbulu, were collected from two locations in KwaZulu-Natal （KZN）, South Africa, and planted at two locations, Pietermaritzburg （KZN） and Roodeplaat, Pretoria. Ago-morphological characterisation of vegetative and corm characteristics were done four months after planting and at harvest, respectively. Sampling for DNA fingerprinting using five SSR primers was done using leaf material four months after planting. Agro-morphological characterisation was useful in showing differences between the wild landrace and the two cultivated landraces, as well as identification of dasheen and eddoe types. SSR primer characterisation showed that despite significant morphological difference, the wild Dumbe Lomfula and Umbumbulu landraces were closely related but different from the KwaNgwanase landrace. Although landraces showed great morphological variation, this did not necessarily imply genetic variation. It is concluded that SSR primers are more useful for characterising taro landraces.展开更多
A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of ta...A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of taro and facilitate cooking. The aim of this study was to assess the effect of traditional soaking on the in vitro digestibility of taro flour using or not using an α-amylase enzyme. The digestion without the enzyme has shown that the soaking processes improve the digestibility of taro flour (from 39.30% for the control sample to 75.11% (after tamarind infusion) and 78.67% (treatment with water) after 24 hours of soaking). Soaking over a 6-hour period and preferentially in tamarind infusion or in corn solution obtains highly digestible flour (around 95% of digestibility rate after 3 hours of enzymatic digestion).展开更多
The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmiss...The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmission and spread of the causal organism of taro leaf blight in taro planting regions.The samples were used to extract total DNA and to be detected by PCR with P.colocasiae specific primer pairs PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.Distinct fragments of about 200 bp and 240 bp were amplified by PCR using primers PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.The analysis of the nucleotide sequence of the PCR products were found to be 99% identical to sequence of RAS-related protein (Ypt1) and phospho-ribosylanthranilate isomerase (TRP1) in P.colocasiae,respectively.It is concluded that rapid and sensitive developed PCR assay for detection of P.colocasiae could be used in routine diagnosis and aid in management practices to mitigate taro leaf blight.展开更多
文摘Taro （Colocasia esculenta L. Schott） is an important underutilised crop in South Africa, East Africa and Indonesia. Three taro landraces, namely, Dumbe Lomfula （wild）, KwaNgwanase and Umbumbulu, were collected from two locations in KwaZulu-Natal （KZN）, South Africa, and planted at two locations, Pietermaritzburg （KZN） and Roodeplaat, Pretoria. Ago-morphological characterisation of vegetative and corm characteristics were done four months after planting and at harvest, respectively. Sampling for DNA fingerprinting using five SSR primers was done using leaf material four months after planting. Agro-morphological characterisation was useful in showing differences between the wild landrace and the two cultivated landraces, as well as identification of dasheen and eddoe types. SSR primer characterisation showed that despite significant morphological difference, the wild Dumbe Lomfula and Umbumbulu landraces were closely related but different from the KwaNgwanase landrace. Although landraces showed great morphological variation, this did not necessarily imply genetic variation. It is concluded that SSR primers are more useful for characterising taro landraces.
基金grateful to Chad French Ambassy for the financing of this project.
文摘A traditional process used by farmers in Chad consists in soaking slices of taro (Colocasia esculenta L. SCHOTT) in tamarind infusion, or in corn solution or in water over a 24-hour period to reduce the acridity of taro and facilitate cooking. The aim of this study was to assess the effect of traditional soaking on the in vitro digestibility of taro flour using or not using an α-amylase enzyme. The digestion without the enzyme has shown that the soaking processes improve the digestibility of taro flour (from 39.30% for the control sample to 75.11% (after tamarind infusion) and 78.67% (treatment with water) after 24 hours of soaking). Soaking over a 6-hour period and preferentially in tamarind infusion or in corn solution obtains highly digestible flour (around 95% of digestibility rate after 3 hours of enzymatic digestion).
基金Sponsored by Science and Technology Major Project of Guangxi(AA17204026)Basic Research Special Fund,and Scientific and Technological Fund of Guangxi Academy of Agricultural Sciences(2016YM20,2018JZ37)Guangxi Natural Science Fund(2016GXNSFAA380195)
文摘The present PCR assay was conducted to develop rapid and sensitive detection of Phytophthora colocasiae,in order to provide a robust and reliable tool for healthy seedling production of taro and limiting the transmission and spread of the causal organism of taro leaf blight in taro planting regions.The samples were used to extract total DNA and to be detected by PCR with P.colocasiae specific primer pairs PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.Distinct fragments of about 200 bp and 240 bp were amplified by PCR using primers PCSP-RL F/PCSP-RL R and PCSP-T F/PCSP-T R,respectively.The analysis of the nucleotide sequence of the PCR products were found to be 99% identical to sequence of RAS-related protein (Ypt1) and phospho-ribosylanthranilate isomerase (TRP1) in P.colocasiae,respectively.It is concluded that rapid and sensitive developed PCR assay for detection of P.colocasiae could be used in routine diagnosis and aid in management practices to mitigate taro leaf blight.
基金National Scientific and Technological Support Planning Program(2012BAD27B07-6)Basic Research Special Fund,and Scientific and Technological Fund of Guangxi Academy of Agricultural Sciences(2015JM15,2015YT55,NCZ2016014)Guangxi Natural Science Fund(2016GXNSFAA380006)
