AIM: There is strong evidence that interleukin-11 (IL-11) is involved in the regulation of tumor progression, cellular growth and differentiation. Recently, interleukin-11 receptor (IL-11R) has been detected on some c...AIM: There is strong evidence that interleukin-11 (IL-11) is involved in the regulation of tumor progression, cellular growth and differentiation. Recently, interleukin-11 receptor (IL-11R) has been detected on some cancer cells. In this study, we investigated the expression of IL-11 and IL-11R in colorectal adenocarcinoma. METHODS: To elucidate the involvement of IL-11 and IL-11Ra in human intestinal adenocarcinomas, we examined 115 cases of surgically resected human colonic adenocarcinoma and 11 cases of adenoma by immuno-histochemistry and Western blotting. RESULTS: Among 115 cases of adenocarcinoma, 100 cases (87.0%) showed positive staining in the cytoplasm of carcinoma cells for the IL-11, and 87 cases (75.6%) were positive for the IL-11Ra. Six cases (54.5%) and four cases (36.4%) of 11 adenomas were positive for IL-11 and IL-11Ra, respectively. The expression of IL-11Ra correlated with the histological differentiation (P=0.033503), the depth of tumor invasion (P=0.006395), Dukes' classification (P= 0.015648) and lymphatic invasion (P=0.003865). However, the expression of IL-11Ra was not correlated with the venous invasion and the presence of lymph node metastasis. The expression of IL-11 was not correlated with any clinicopathological factors. In Western blot analysis, two human colorectal carcinoma cell lines and four tissues of surgically resected human carcinoma expressed both IL-11 and IL-11Ra proteins. CONCLUSION: IL-11 and IL-11Ra are highly expressed in human colorectal adenocarcinoma and the IL-11Ra expression is correlated with clinicopathological factors. These findings suggest that the expression of IL-11Ra is an important factor for the invasion of human colorectal adenocarcinoma.展开更多
目的检测富含脯氨酸蛋白11(proline-rich protein 11,PRR11)在结直肠癌中的表达,探讨其对结直肠癌侵袭和转移的影响。方法应用免疫组织化学检测2015年1月至2018年3月重庆市江津区中心医院136例结直肠癌组织与68例癌旁组织中PRR11的表达;...目的检测富含脯氨酸蛋白11(proline-rich protein 11,PRR11)在结直肠癌中的表达,探讨其对结直肠癌侵袭和转移的影响。方法应用免疫组织化学检测2015年1月至2018年3月重庆市江津区中心医院136例结直肠癌组织与68例癌旁组织中PRR11的表达;Western blot、RT-PCR及免疫荧光检测PRR11及EMT相关信号分子在结直肠癌细胞中的表达;细胞划痕实验、Transwell小室法检测细胞的迁移、侵袭能力。结果结直肠癌组织中PRR11阳性表达率高于癌旁组织(60.29%vs 1.47%,P<0.01),且与结直肠癌的远处转移、瘤体大小、TNM分期有关(P<0.05);相比于正常结直肠上皮细胞,PRR11在结直肠癌细胞中高表达(P<0.05);过表达PRR11后,癌细胞HT29的迁移和侵袭能力增强,同时伴随明显的β-catenin核转位,且显著增强β-catenin和Vimentin的表达,抑制E-cadherin的表达(P<0.05);相反,沉默PRR11后,HT29的迁移和侵袭能力减弱,且显著抑制β-catenin和Vimentin的表达,增强E-cadherin的表达(P<0.05)。结论 PRR11在结直肠癌中高表达,且可能通过激活Wnt/β-catenin信号通路,促进β-catenin核转位,从而促进结直肠癌的侵袭和转移。展开更多
文摘AIM: There is strong evidence that interleukin-11 (IL-11) is involved in the regulation of tumor progression, cellular growth and differentiation. Recently, interleukin-11 receptor (IL-11R) has been detected on some cancer cells. In this study, we investigated the expression of IL-11 and IL-11R in colorectal adenocarcinoma. METHODS: To elucidate the involvement of IL-11 and IL-11Ra in human intestinal adenocarcinomas, we examined 115 cases of surgically resected human colonic adenocarcinoma and 11 cases of adenoma by immuno-histochemistry and Western blotting. RESULTS: Among 115 cases of adenocarcinoma, 100 cases (87.0%) showed positive staining in the cytoplasm of carcinoma cells for the IL-11, and 87 cases (75.6%) were positive for the IL-11Ra. Six cases (54.5%) and four cases (36.4%) of 11 adenomas were positive for IL-11 and IL-11Ra, respectively. The expression of IL-11Ra correlated with the histological differentiation (P=0.033503), the depth of tumor invasion (P=0.006395), Dukes' classification (P= 0.015648) and lymphatic invasion (P=0.003865). However, the expression of IL-11Ra was not correlated with the venous invasion and the presence of lymph node metastasis. The expression of IL-11 was not correlated with any clinicopathological factors. In Western blot analysis, two human colorectal carcinoma cell lines and four tissues of surgically resected human carcinoma expressed both IL-11 and IL-11Ra proteins. CONCLUSION: IL-11 and IL-11Ra are highly expressed in human colorectal adenocarcinoma and the IL-11Ra expression is correlated with clinicopathological factors. These findings suggest that the expression of IL-11Ra is an important factor for the invasion of human colorectal adenocarcinoma.