Originated from nature and used for nature is a way of sustainable development.In this work,montmorillonite(MMT),a natural two-dimensional(2D)layered mineral,the surface and interlayer of which were nano-decorated by ...Originated from nature and used for nature is a way of sustainable development.In this work,montmorillonite(MMT),a natural two-dimensional(2D)layered mineral,the surface and interlayer of which were nano-decorated by chemical synthesis technique was applied in biological detection field.Magnetic ferrite(Co_(0.5)Ni_(0.5)Fe_(2)O_(4))was anchored on the surface and intercalated in the interlayer of montmorillonite,which served as a competitive candidate of enzyme mimics.Cytotoxicity test toward HUVEC and Hela cells verified the good biocompatibility of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT,guaranteeing its safety in biological applications.Based on the peroxidase-like activity of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT,a colorimetric sensing platform for H_(2)O_(2) was established by a facile mix-anddetect approach with the detection limit of 0.565μM(3σ/slope).It was implied that the peroxidase-like activity of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT was originated from generation of·OH and·O_(2)–produced from catalytic decomposition process of H_(2)O_(2).Coupled with cascaded catalytic reactions of ACh,a facile and efficient sensing platform for ACh with satisfactory anti-interference ability was established.Thus,all these remarkable features highlighted the superiority of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT,and endowed it with a powerful competitiveness in the fields of environmental assessing,biosensing,and disease monitoring.展开更多
基金supported by the National Natural Science Foundation of China(Nos.51904175 and 21971152)Project of Shandong Province Higher Educational Young Innovative Talent Introduction and Cultivation Team[Nanozymes biomedical innovation team],Scientific and Technological Innovation Foundation of Foshan(BK20BE009)the Fundamental Research Funds for the Central Universities(FRF-TP-18-079A1).
文摘Originated from nature and used for nature is a way of sustainable development.In this work,montmorillonite(MMT),a natural two-dimensional(2D)layered mineral,the surface and interlayer of which were nano-decorated by chemical synthesis technique was applied in biological detection field.Magnetic ferrite(Co_(0.5)Ni_(0.5)Fe_(2)O_(4))was anchored on the surface and intercalated in the interlayer of montmorillonite,which served as a competitive candidate of enzyme mimics.Cytotoxicity test toward HUVEC and Hela cells verified the good biocompatibility of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT,guaranteeing its safety in biological applications.Based on the peroxidase-like activity of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT,a colorimetric sensing platform for H_(2)O_(2) was established by a facile mix-anddetect approach with the detection limit of 0.565μM(3σ/slope).It was implied that the peroxidase-like activity of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT was originated from generation of·OH and·O_(2)–produced from catalytic decomposition process of H_(2)O_(2).Coupled with cascaded catalytic reactions of ACh,a facile and efficient sensing platform for ACh with satisfactory anti-interference ability was established.Thus,all these remarkable features highlighted the superiority of Co_(0.5)Ni_(0.5)Fe_(2)O_(4)-MMT,and endowed it with a powerful competitiveness in the fields of environmental assessing,biosensing,and disease monitoring.
