Commelina communis L. growing over some new copper mining wastelands at Bijiashan, Tongling City of Anhui Province, China, was found to be a copper hyperaccumulator. Its copper concentrations were 2707-6159 (4439±...Commelina communis L. growing over some new copper mining wastelands at Bijiashan, Tongling City of Anhui Province, China, was found to be a copper hyperaccumulator. Its copper concentrations were 2707-6159 (4439±2434) mg kg-1, 369-831 (731±142) mg kg-1, and 429-587 (547±57) mg kg-1, respectively, in the roots, stems, and leaves. The soils supporting the growth of the species had a copper concentration ranging from 4620 to 5020 mg kg-1 and averaging 4835±262 mg kg-1, suggesting that the species could not only grow on heavily copper-contaminated soils but also accumulate extraordinarily high concentration of copper. Thus, it shows great potential in the phytoremediation of copper-contaminated soils,the restoration of mined land, geochemical prospecting, and the study of environmental pollution changes.展开更多
Stomatal aperture responses of Commelina communis L. between well watered plants and water stressed plants were investigated. To see the very rapid response to water stress, the plants were directly rooted out from th...Stomatal aperture responses of Commelina communis L. between well watered plants and water stressed plants were investigated. To see the very rapid response to water stress, the plants were directly rooted out from the soil and exposed to the air immediately. Stomata, rooted out from the soil, were totally closed within 10 minutes without any detention time while the stomata of the plants in the soil had been kept opening. These results suggest that stomatal response to the abrupt water stress is very rapid indicating that guard cell itself or leaves could sense water status in the plants.展开更多
目的:基于网络药理学和分子对接技术探究鸭跖草治疗高热惊厥的作用机制。方法:通过检索中药系统药理学(the Chinese Medicine System Pharmacology,TCMSP)数据库、BATMAN-TCM数据库提取鸭跖草的有效成分并提取相关作用靶点,通过GeneCard...目的:基于网络药理学和分子对接技术探究鸭跖草治疗高热惊厥的作用机制。方法:通过检索中药系统药理学(the Chinese Medicine System Pharmacology,TCMSP)数据库、BATMAN-TCM数据库提取鸭跖草的有效成分并提取相关作用靶点,通过GeneCards和OMIM数据库检索高热惊厥疾病靶点。运用Uniport将蛋白与基因symbol转换,通过Darw venn diagram平台得出venn图及药物成分与疾病的交集基因。通过STRING数据库构建PPI网络。利用Cytoscape3.9.1软件构建“中药-有效成分-靶点”网络及“关键靶点-信号通路”网络,采用在线分析平台DAVID v6.8对关键靶点进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析和基因本体(gene ontology,GO)富集分析。利用软件R x644.0.2及绘图包ggplot2绘制KEGG信号通路和GO富集分析图。利用AutoDockTools 1.5.7,Pymol 2.0.1和Openbable3.1.1软件完成分子对接。结果:鸭跖草主要有效成分8种,对应靶点140个,疾病靶点2210个,药物疾病共同靶点64个,GO富集分析得到262条目(P<0.05)、KEGG通路富集分析筛选出67条信号通路(P<0.05),分子对接的结果显示,鸭跖草的活性成分黄酮类化合物主要通过3QXY,1GFW和2K7W等靶点调节多条信号通路发挥抗高热惊厥作用。结论:鸭跖草中的黄酮、β-谷甾醇和丙二酸单酰基人参皂苷Rb2等成分可与高热惊厥疾病靶点稳定结合并且结合能力强于临床上用于治疗高热惊厥的苯巴比妥,鸭跖草可通过多成分、多靶点、多通路治疗高热惊厥,本研究为鸭跖草用于高热惊厥治疗的研究与开发提供了新的思路。展开更多
目的研究鸭跖草Commelina communis L.的化学成分。方法鸭跖草95%乙醇提取物采用硅胶、MCI、Sephadex LH-20、HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到15个化合物,分别鉴定为银杏内酯A(1)、银...目的研究鸭跖草Commelina communis L.的化学成分。方法鸭跖草95%乙醇提取物采用硅胶、MCI、Sephadex LH-20、HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到15个化合物,分别鉴定为银杏内酯A(1)、银杏内酯B(2)、(3S,5R,6S,7E)-3,5,6-thriydroxy-7-megastigmen-9-one(3)、金松双黄酮(4)、木犀草素(5)、山柰酚(6)、3-醛基吲哚(7)、对羟基苯甲醛(8)、丁香醛(9)、香草乙酮(10)、原儿茶酸(11)、对羟基苯甲酸(12)、棕榈酸(13)、β-谷甾醇(14)、豆甾醇(15)。结论化合物1~4、8~10、13为首次从鸭跖草属及该植物中分离得到。展开更多
文摘Commelina communis L. growing over some new copper mining wastelands at Bijiashan, Tongling City of Anhui Province, China, was found to be a copper hyperaccumulator. Its copper concentrations were 2707-6159 (4439±2434) mg kg-1, 369-831 (731±142) mg kg-1, and 429-587 (547±57) mg kg-1, respectively, in the roots, stems, and leaves. The soils supporting the growth of the species had a copper concentration ranging from 4620 to 5020 mg kg-1 and averaging 4835±262 mg kg-1, suggesting that the species could not only grow on heavily copper-contaminated soils but also accumulate extraordinarily high concentration of copper. Thus, it shows great potential in the phytoremediation of copper-contaminated soils,the restoration of mined land, geochemical prospecting, and the study of environmental pollution changes.
文摘Stomatal aperture responses of Commelina communis L. between well watered plants and water stressed plants were investigated. To see the very rapid response to water stress, the plants were directly rooted out from the soil and exposed to the air immediately. Stomata, rooted out from the soil, were totally closed within 10 minutes without any detention time while the stomata of the plants in the soil had been kept opening. These results suggest that stomatal response to the abrupt water stress is very rapid indicating that guard cell itself or leaves could sense water status in the plants.
文摘目的:基于网络药理学和分子对接技术探究鸭跖草治疗高热惊厥的作用机制。方法:通过检索中药系统药理学(the Chinese Medicine System Pharmacology,TCMSP)数据库、BATMAN-TCM数据库提取鸭跖草的有效成分并提取相关作用靶点,通过GeneCards和OMIM数据库检索高热惊厥疾病靶点。运用Uniport将蛋白与基因symbol转换,通过Darw venn diagram平台得出venn图及药物成分与疾病的交集基因。通过STRING数据库构建PPI网络。利用Cytoscape3.9.1软件构建“中药-有效成分-靶点”网络及“关键靶点-信号通路”网络,采用在线分析平台DAVID v6.8对关键靶点进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析和基因本体(gene ontology,GO)富集分析。利用软件R x644.0.2及绘图包ggplot2绘制KEGG信号通路和GO富集分析图。利用AutoDockTools 1.5.7,Pymol 2.0.1和Openbable3.1.1软件完成分子对接。结果:鸭跖草主要有效成分8种,对应靶点140个,疾病靶点2210个,药物疾病共同靶点64个,GO富集分析得到262条目(P<0.05)、KEGG通路富集分析筛选出67条信号通路(P<0.05),分子对接的结果显示,鸭跖草的活性成分黄酮类化合物主要通过3QXY,1GFW和2K7W等靶点调节多条信号通路发挥抗高热惊厥作用。结论:鸭跖草中的黄酮、β-谷甾醇和丙二酸单酰基人参皂苷Rb2等成分可与高热惊厥疾病靶点稳定结合并且结合能力强于临床上用于治疗高热惊厥的苯巴比妥,鸭跖草可通过多成分、多靶点、多通路治疗高热惊厥,本研究为鸭跖草用于高热惊厥治疗的研究与开发提供了新的思路。
文摘目的研究鸭跖草Commelina communis L.的化学成分。方法鸭跖草95%乙醇提取物采用硅胶、MCI、Sephadex LH-20、HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到15个化合物,分别鉴定为银杏内酯A(1)、银杏内酯B(2)、(3S,5R,6S,7E)-3,5,6-thriydroxy-7-megastigmen-9-one(3)、金松双黄酮(4)、木犀草素(5)、山柰酚(6)、3-醛基吲哚(7)、对羟基苯甲醛(8)、丁香醛(9)、香草乙酮(10)、原儿茶酸(11)、对羟基苯甲酸(12)、棕榈酸(13)、β-谷甾醇(14)、豆甾醇(15)。结论化合物1~4、8~10、13为首次从鸭跖草属及该植物中分离得到。
