Effect of Commiphora mukul gum resin on hepatic marker enzymes,lipid peroxidation and antioxidants status in pancreas and heart of streptozotocin induced diabetic rats

Objective:To study the antioxidant efficacy of Commiphora mukul(C.mukul) gum resin ethanolic extract in streptozotocin(STZ) induced diabetic rats.Methods:The male Wistar albino rats were randomly divided into four groups of eight animals each:Control group(C),CM-treated control group(C_+CMEE),Diabetic control group(D),CM- treated diabetic group(D_+CMEE).Diabetes was induced by intraperitoneal injection of STZ(55 mg/kg/ bwt).After being confirmed the diabetic rats were treated with C.mukul gum resin ethanolic extract(CMEE) for 60 days.The biochemical estimations like antioxidant,oxidative stress marker enzymes and hepatic marker enzymes of tissues were performed.Results:The diabetic rats showed increased level of enzymatic activities aspartate aminotransaminase(AST),alanine aminotransaminase(ALT) in liver and kidney and oxidative markers like lipid peroxidation(LPO) and protein oxidation(PO) in pancreas and heart. Antioxidant enzyme activities were significantly decreased in the pancreas and heart compared to control group.Administration of CMEE(200 mg/kg bw) to diabetic rats for 60 days significantly reversed the above parameters towards normalcy.Conclusions:In conclusion,our data indicate the preventive role of C.mukul against STZ-induced diabetic oxidative stress;hence this plant could be used as an adjuvant therapy for the prevention and/or management of diabetes and aggravated antioxidant status.

穆库没药化学成分的研究

目的:对穆库没药化学成分进行系统研究。方法:采用硅胶柱色谱,ODS柱色谱及制备HPLC等方法分离纯化,运用现代光谱学方法鉴定化合物结构。结果:从穆库没药中分离得到4个化合物,分别鉴定为Epi-magnolin(1)、1,7,8,10,11,12,13,14,15,17-decahydro-17-decahydro-17-(S)-1-hydroxyethyl-10,13-dimethyl-2H-cyclopenta[α]phenanthrene-3,16(16H,9H)-dione(2)、1,7,8,10,11,12,13,15,16,17-decahydro-17-(2-hydroxy-6-methylheptan-2-yl)-10,13-dimethyl-2H-cyclopenta[α]phenanthrene-3(6H,9H,14H)-one(3)、20-methoxy-4,17(20)Z-pregnadiene-3,16-dione(4)。结论:化合物1～3从橄榄科中首次分离得到,化合物4从该植物中首次分离得到。

穆库没药甾体类化学成分的研究

目的:对穆库没药甾体类化学成分进行系统研究。方法:采用硅胶柱色谱、ODS柱色谱及制备HPLC等方法分离纯化,运用现代光谱学方法鉴定化合物结构。结果:从穆库没药中分离得到5个化合物,分别鉴定为4,17(20)-(cis)-pregnad-iene-3,16-dione(1)、progesterone(2)、20-acetyloxy4-pregnene-3,16-dione(3)、Z-4,17(20)-pregnadiene-3,16-dione(Z-Gugguls-terone)(4)、E4,17(20)-pregnadiene-3,16-dione(E-Guggulsterone)(5)。结论:化合物1-2从穆库没药中首次分离得到。

黑云香的研究进展

蒙药黑云香为蒙古族习用药材,主要含有萜类、甾体类、木质素类等成分,具有抗炎、抗菌、抗氧化、镇痛以及降血脂等药理活性。

穆库尔没药中的2个新吉玛烷型倍半萜

目的研究穆库尔没药Commiphora mukul树脂的化学成分。方法采用硅胶和Sephadex LH-20凝胶柱色谱法进行分离纯化,运用波谱学方法鉴定化合物的结构。结果从穆库尔没药树脂醋酸乙酯提取物中分离获得2个吉玛烷型倍半萜化合物,分别鉴定为1α,8α,12α-三羟基-2β-甲氧基-8,12-环氧吉玛烷-7(11),9-二烯-6-酮(1)和1α,8β,12α-三羟基-2β-甲氧基-8,12-环氧吉玛烷-7(11),9-二烯-6-酮(2)。结论 2个吉玛烷型倍半萜化合物1和2为未见文献报道的新化合物,分别命名为穆库尔素A(1)和穆库尔素B(2)。