Library-Inside:AI赋能图书馆新质生产力的一种基础模型

AI正引发知识服务的生产力×生产关系的革命性变革,迫切需要推动AI与图书馆服务深度融合,打造符合新的发展理念的先进生产力质态。本文提出,从第一性原则思维出发,面向复杂适应性系统,以问题驱动和场景驱动的创新和设计驱动的创新,不断演进发展AI赋能的图书馆服务的新质生产力。在回归原点分析“人们到图书馆干什么”的基础上,基于大语言模型+AI智能体提供的能力,提出Library-Inside作为AI赋能图书馆新质生产力的一种基础模型,并描述了三种可能的实现模式:Library-Inside 1.0作为用户知识应用的智能助手,Library-Inside 2.0作为用户知识生产的智慧座舱和Library-Inside 3.0(Library×)打造用户AI赋能知识生产力的生态养育站。本文指出,要以战略创新推动图书馆新质生产力发展,要注意克服战略创新中的内在的结构性缺失和系统性偏差。

An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from scratch.Exogenous genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional mutation.The random insertional mutant library constructed using this method has become a method of forward genetics for gene cloning.However,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous genes.Many microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries difficult.In this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was identified.This method will facilitate the genetic studies of N.oceanica,which should also be a reference for other microalgal species.

The Book Publishing and Analysis of Modern Peking University Library

The paper is based on literature search,introducing and analyzing the book publishing situation of modern Peking University Library from the perspective of the publisher being Peking University Library and its predecessor.It is pointed out that the majority of the book printing methods published by the modern Peking University Library are lead printing,and among the types of Chinese library classification,catalogue of library collections is the most common.

Other Cities’Distribution Institutions for Publishing Books in Modern Chinese Libraries:Taking Huating Bookstore,the Shanghai General Distribution Office of Modern Zhejiang Library,as an Example

Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zhejiang Library in modern times,is one of them.On the basis of a brief description of modern Zhejiang Library and its book publishing,as well as the overview of Huating Bookstore,the paper introduces and analyzes the ZPL Publishing Book Catalogue sent by Huating Bookstore,and the ZPL publishing and selling consignment books issued by Huating Bookstore.It points out that Huating Bookstore is a bridge between the ZPL located in Hangzhou and various retail ZPL publishing bookstores in Shanghai.Their production and sales relationship is a mutually beneficial one.

Integrated Project Library Exploration in Landscape Design Teaching for Environmental Design Majors

To enhance our talent cultivation model through“school-enterprise cooperation and industry-teaching fusion”,we aim to improve the“4321”Industry-Education Integration System.This includes actively promoting the use of case banks and project banks in teaching to develop students’practical engineering skills through hands-on application of professional knowledge.Additionally,landscape design courses emphasize practical learning experiences to implement the fundamental goal of“cultivating morality”.Guided by enhancing students’practical skills,we ensure alignment with course objectives and professional training requirements,emphasizing the seamless integration of theory and practice.

Analysis of the Books Issued by Yadong Library, Published by Peking University Publishing Department: Taking Several Bibliography Advertisements Carried in Newspapers and Magazines From 1919-1922 as Examples

Based on several bibliographic advertisements carried in newspapers and magazines from 1919 to 1922 for books published by Peking University Publishing Department,and issued by Yadong Library,the paper analyzes the characteristics of the books published by Peking University Publishing Department during this period,and points out that Yadong Library entered its golden period of development due to move from the alley to the main road and obtain the management rights of the books published by Peking University Publishing Department.

高校图书馆员数据素养提升策略——来自Library carpentry课程项目的经验

通过网络调查和文献调研分析了图书馆员的数据素养提升的Library carpentry课程项目,从发起背景、课程团队、经费支持、课程目标、课程内容等方面调查了Library carpentry的管理和运行实践,从项目组织、课程和教学方面总结出Library carpentry项目的特点。根据Library carpentry的成功经验,从开拓图书馆服务视野、打造实用化课程体系、推动教育可持续发展,搭建协同教育平台等角度思考对我国图书馆员数字素养教育的启示。

Linker Library:基于几何特征的骨架跃迁片段库

骨架跃迁是目前应用最广泛的药物设计策略之一,但是现有骨架跃迁方法产生的化合物大多为已报道的先导化合物的衍生物或类似物,化学结构缺乏新颖性。针对现有骨架跃迁方法的局限性,提出了一种保持药效团与骨架之间的相对距离和角度不变的骨架跃迁策略,构建一个包含骨架几何特征的linker片段库:Linker Library。该片段库可以根据骨架中心到连接点之间距离和角度,推荐结构新颖且可保持官能团相对位置的片段,有助于指导化合物的骨架跃迁并加速药物发现进程。

Small Amplicons Mutation Library for Vaccine Screening by Error-Prone Polymerase Chain Reaction

Library construction is a common method used to screen target genes in molecular biology.Most library constructions are not suitable for a small DNA library(<100 base pair(bp))and low RNA library output.To maximize the library’s complexity,error-prone polymerase chain reaction(PCR)was used to increase the base mutation rate.After introducing the DNA fragments into the competent cell,the library complexity could reach 109.Library mutation rate increased exponentially with the dilution and amplification of error-prone PCR.The error-prone PCR conditions were optimized including deoxyribonucleotide triphosphate(dNTP)concentration,Mn^(2+)concentration,Mg^(2+)concentration,PCR cycle number,and primer length.Then,a RNA library with high complexity can be obtained by in vitro transcription to meet most molecular biological screening requirements,and can also be used for mRNA vaccine screening.

Dock-able linear and homodetic di, tri, tetra and pentapeptide library from canonical amino acids: SARS-CoV-2 Mpro as a case study

Peptide-based therapeutics are increasingly pushing to the forefront of biomedicine with their promise of high specificity and low toxicity.Although noncanonical residues can always be used,employing only the natural 20 residues restricts the chemical space to a finite dimension allowing for comprehensive in silico screening.Towards this goal,the dataset comprising all possible di-,tri-,and tetra-peptide combinations of the canonical residues has been previously reported.However,with increasing computational power,the comprehensive set of pentapeptides is now also feasible for screening as the comprehensive set of cyclic peptides comprising four or five residues.Here,we provide both the complete and prefiltered libraries of all di-,tri-,tetra-,and penta-peptide sequences from 20 canonical amino acids and their homodetic(N-to-C-terminal)cyclic homologues.The FASTA,simplified molecular-input line-entry system(SMILES),and structure-data file(SDF)-three dimension(3D)libraries can be readily used for screening against protein targets.We also provide a simple method and tool for conducting identity-based filtering.Access to this dataset will accelerate small peptide screening workflows and encourage their use in drug discovery campaigns.As a case study,the developed library was screened against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)main protease to identify potential small peptide inhibitors.

Identification and epitope mapping of anti-p72 single-chain antibody against African swine fever virus based on phage display antibody library

African swine fever virus(ASFV)is a lethal pathogen that causes severe threats to the global swine industry and it has already had catastrophic socio-economic effects.To date,no licensed prophylactic vaccine exists.Limited knowledge exists about the major immunogens of ASFV and the epitope mapping of the key antigens.As such,there is a considerable requirement to understand the functional monoclonal antibodies(mAbs)and the epitope mapping may be of utmost importance in our understanding of immune responses and designing improved vaccines,therapeutics,and diagnostics.In this study,we generated an ASFV antibody phage-display library from ASFV convalescent swine PBMCs,further screened a specific ASFV major capsid protein(p72)single-chain antibody and fused with an IgG Fc fragment(scFv-83-Fc),which is a specific recognition antibody against ASFV Pig/HLJ/2018 strain.Using the scFv-83-Fc mAb,we selected a conserved epitope peptide(221MTGYKH226)of p72 retrieved from a phage-displayed random peptide library.Moreover,flow cytometry and cell uptake experiments demonstrated that the epitope peptide can significantly promote BMDCs maturation in vitro and could be effectively uptaken by DCs,which indicated its potential application in vaccine and diagnostic reagent development.Overall,this study provided a valuable platform for identifying targets for ASFV vaccine development,as well as to facilitate the optimization design of subunit vaccine and diagnostic reagents.

Construction of Smart Library Based on the Agricultural Information from Metaverse Horizon

Metaverse is a virtual world,but it is possible and even becomes a trend that metaverse interacts with the real world along with the sci-tech and social development.Smart library is a main direction of modern library development,from informatization to digitization and then intelligent-based,modernization of library has been improved qualitatively.Emergence of metaverse brings a brand-new horizon to the generation and application of agricultural information,this paper made primary explorations the requirements and action mechanism of agricultural information on the construction of smart library within the external constraint of metaverse.

Research on the Value Positioning of University Library Based on Customer Delivered Value

With the rise of various new reading media and the rapid development of the digitization of paper resources,the traditional function positioning of university libraries based on information query is facing unprecedented challenges.How to deal with the change of social information?The library should be demand-oriented,re-examine its own value and find a new starting point.With the help of the concept of customer delivered value and based on 4P theory,this paper constructs the value chain of university library from the dimensions of product,image,personnel and service,and forms a multi-dimensional development positioning system.

Behavioral Intention to Continue Using a Library Mobile App

To meet the needs of today’s library users,institutions are developing library mobile apps(LMAs),as their libraries are increasingly intelligent and rely on deep learning.This paper explores the influencing factors and differences in the perception of LMAs at different time points after a user has downloaded an LMA.A research model was constructed based on the technology acceptance model.A questionnaire was designed and distributed twice to LMA users with an interval of three months to collect dynamic data.The analysis was based on structural equation modeling.The empirical results show that the perceived ease of use,the perceived usefulness,the social influence,and the facilitating conditions affected the users’behavioral intention,but their impacts were different at different times.As the usage time increases,the technology acceptance model is still universal for understanding the user perception of LMA.In addition,two extended variables(social impact and convenience)also affect the user’s behavior intention.User behavior is dynamic and changed over time.This study is important both theoretically and practically,as the results could be used to improve the service quality of LMAs and reduce the loss rate of users.Its findings may help the designers and developers of LMAs to optimize them from the perspective of a user and improve the service experience by providing a deeper understanding of the adoption behavior of information systems by LMA users.

加强用户关系管理,提升图书馆服务质量--以东南大学图书馆为例

“以用户为中心”是图书馆开展服务的核心理念。东南大学图书馆基于用户关系管理相关理论,开展用户关系管理的实践探索,细分用户、掌握需求,多渠道建立用户强交互关系,在用户参与、用户受益等方面取得了较好的成果,期望能为高校图书馆同行开展相关服务提供经验参考。

Problems and Countermeasures in the Informatization of Archives Management in University Library

To improve the level of informatization of university library archives management,the problems existing in the information construction of university library archives management are analyzed and some practical solutions are proposed in this paper.These solutions can effectively reduce the investment in human resources and equipment and facilities and improve the utilization rate of resources for computer application of university library archives management.

“文化活化”视域下高校图书馆古籍活化实践路径探索--以浙江大学图书馆馆藏拓片活化为例

在“文化强国”建设的背景下,古籍活化成为古籍保护与利用工作中的重要任务。文章提出了一种新的以文化活化促进古籍活化的特藏揭示模式,并以浙江大学图书馆拓片活化应用实践为例,总结了高校图书馆古籍活化的五种实践路径及“4C”策略,以期为古籍活化工作提供新思路。

生成式AI视域下智慧图书馆建设的关键路径

[目的/意义]智慧图书馆已经成为当今图书馆学界和业界最热门的主题之一。智慧图书馆建设的理论研究与实践工作已经取得了初步成效,生成式AI也在蓬勃发展的同时参与到各行各业的建设中。因此有必要立足当下发展,放眼远景目标,确定生成式AI参与智慧图书馆建设的策略。[方法/过程]本文从智慧图书馆空间升级、服务打造、业务重组、人才培养、体系建设5个方面阐明了生成式AI视域下智慧图书馆建设的关键路径。[结果/结论]关键路径研究将为生成式AI引入智慧图书馆下一阶段的建设工作提供借鉴与依据。

Construction of Ship Target Image Library Based on 3DS MAX and AP Algorithm

To achieve accurate classification and recognition of ship target types,it is necessary to establish a sample library of ship targets to be identified.On the basis of exploring the principles of building a ship target image library,the paper determines the sample set.Using 3DS MAX software as the platform,combined with the accurate 3D model of the ship in an offline state,the software fully utilizes its own rendering and animation functions to achieve the automatic generation of multi-view and multi-scale views of ship targets.To reduce the storage capacity of the image database,a construction method of the ship target image database based on the AP algorithm is presented.The algorithm can obtain the optimal cluster number,reduce the data storage capacity of the image database,and save the calculation amount for the subsequent matching calculation.

Research on the Construction of a Landscape Engineering Teaching Resource Library Under the“4321”Industry-Education Integration System

This paper studies and explores the construction of a teaching resource library for landscape engineering under the“4321”industry-education integration system,focusing on the construction objectives,construction paths,and construction contents,aiming to open-up the channel of school-enterprise cooperation,improve enterprise participation,and promote the reform of teaching mode and teaching methods.Establishing a practical resource library will connect and integrate students,teachers,and enterprises.A complete library of landscape-oriented teaching resources will be constructed according to the talent portraits of the industry to open-up the relationship between upstream and downstream courses,and further improve the precise fit between the cultivation of applied undergraduate talents and enterprises.