Features of animal models of complex partial epilepsy established through unilateral,bilateral and alternate-side kindling at hippocampus of rats

BACKGROUND： Electrical stimulation kindling model, having epilepsy-inducing and spontaneous seizure and other advantages, is a very ideal experimental animal model. But the kindling effect might be different at different sites. OBJECTIVE： To compare the features of animal models of complex partial epilepsy established through unilateral, bilateral and alternate-side kindling at hippocampus and successful rate of modeling among these 3 different ways. DESIGN： A randomized and controlled animal experiment SETTING： Department of Neurology, Qilu Hospital, Shandong University MATERIALS： Totally 60 healthy adult Wistar rats, weighing 200 to 300 g, of either gender, were used in this experiment. BL-410 biological functional experimental system （Taimeng Science and Technology Co. Ltd, Chengdu） and SE-7102 type electronic stimulator （Guangdian Company, Japan） were used in the experiment. METHODS： This experiment was carried out in the Experimental Animal Center of Shandong University from April to June 2004. After rats were anesthetized, electrode was implanted into the hippocampus. From the first day of measurement of afterdischarge threshold value, rats were given two-square-wave suprathreshold stimulation once per day with 400 μA intensity, 1ms wave length, 60 Hz frequency for 1 s duration. Left hippocampus was stimulated in unilateral kindling group, bilateral hippocampi were stimulated in bilateral kindling group, and left and right hippocampi were stimulated alternately every day in the alternate-side kindling group. Seizure intensity was scored： grade 0： normal, 1： wet dog-like shivering, facial spasm, such as, winking, touching the beard, rhythmic chewing and so on; 2： rhythmic nodding; 3： forelimb spasm;4： standing accompanied by bilateral forelimb spasm;5： tumbling, losing balance, four limbs spasm. Modeling was successful when seizure intensity reached grade 5. t test was used for the comparison of mean value between two samples. MAIN OUTCOME MEASURES： Comparison of the successful rate of modeling, the times of stimulation to reach intensity of grade 5, the lasting time of seizure of grade 3 of rats in each group. RESULTS： Four rats of alternate-side kindling group dropped out due to infection-induced electrode loss, and 56 rats were involved in the result analysis. The successful rate of unilateral kindling group, bilateral kin- dling group and alternate-side kindling group was 55%（11/20）,100%（16/16）and 100%（20/20）, respective- ly. The stimuli to reach the grade 5 spasm were significantly more in the bilateral kindling group than in the unilateral kindling group [（30.63±3.48）, （19.36±3.47）times, t=8.268, P 〈 0.01], and those were significantly fewer in the alternate-side kindling group than in the unilateral kindling group [（ 10.85±1.98）times, t=-8.744, P 〈 0.01]. The duration of grade 3 spasm was significantly longer in the bilateral kindling group than in the unilateral kindling group [（9.75±2.59）, （3.21 ±1.58）days,t=-8.183,P 〈 0.01], Among 20 successful rats of al- ternate-side kindling group, grade 5 spasm was found in the left hippocampi of 11 rats, but grade 3 spasm in their right hippocampi; Grade 5 spasm was found in the right hippocampi of the other 9 rats, grade 4 spasm in the left hippocampus of 1 rat and grade 3 of 8 rats. CONCLUSION ： The speed of establishing epilepsy seizure model by alternate-side kindling is faster than that by unilateral kindling, while that by bilateral kindling is slower than that by unilateral kindling. The successful rate is very high to establish complex partial epilepsy with alternate-side or bilateral kindling. Epilepsy seizure established by alternate-side kindling has antagonistic effect of kindling and the seizure duration of grade 3 spasm is prolonged.

3D simulation of image-defined complex internal features using the numerical manifold method

The numerical simulation of internal features,such as inclusions and voids,is important to analyze their impact on the performance of composite materials.However,the complex geometries of internal features and the induced continuous-discontinuous(C-D)deformation fields are challenges to their numerical simulation.In this study,a 3D approach using a simple mesh to simulate irregular internal geometries is developed for the first time.With the help of a developed voxel crack model,image models that are efficient when recording complex geometries are directly imported into the simulation.Surface reconstructions,which are usually labor-intensive,are excluded from this approach.Moreover,using image models as the geometric input,image processing techniques are applied to detect material interfaces and develop contact pairs.Then,the C-D deformations of the complex internal features are directly calculated based on the numerical manifold method.The accuracy and convergence of the developed3D approach are examined based on multiple benchmarks.Successful 3D C-D simulation of sandstones with naturally formed complex microfeatures demonstrates the capability of the developed approach.

Probability Distribution and Their Non-linear Relationship between Node Degree and Clustering Coefficient of Aviation Network of China Based on Complex Network

In order to reveal the complex network feature of aviation network of China,probability distribution of node degree and clustering coefficient of aviation network of China was researched according to statistics data of civil aviation of China.It was verified that node degree had power function probability distribution.Clustering coefficient of nodes with exponential function probability distribution was discovered.It was found that node degree and clustering coefficient had single peak nonlinear relationship.At the left side of the peak,there is no certain relationship between them.At the right side of the peak,clustering coefficient became smaller with the rise of node degree and there was negative exponential function relationship between them by regression analysis.

Crystal structure of the coxsackievirus A16 RNA-dependent RNA polymerase elongation complex reveals novel features in motif A dynamics

Dear Editor,Coxsackievirus A16(CV A16)and enterovirus 71(EV71)are currently the two primary causative agents of handfoot-and-mouth disease(HFMD)(Solomon et al.,2010;Mao et al.,2014),threatening health of children worldwide.They both belong to the Enterovirus genus of

Photonics Research Feature Announcement Non-Hermitian Photonics in Complex Media: PT-symmetry and beyond

Submission Open:15 March 2017 Submission Deadline:15 April 2017 The idea of parity-time(PT)symmetry,first introduced in quantum mechanics,was recently realized in the context of photonics in the form of balanced gain-loss structures with special symmetries.In recent years。

Measuring air traffic complexity based on small samples

Air traffic complexity is an objective metric for evaluating the operational condition of the airspace. It has several applications, such as airspace design and traffic flow management.Therefore, identifying a reliable method to accurately measure traffic complexity is important. Considering that many factors correlate with traffic complexity in complicated nonlinear ways,researchers have proposed several complexity evaluation methods based on machine learning models which were trained with large samples. However, the high cost of sample collection usually results in limited training set. In this paper, an ensemble learning model is proposed for measuring air traffic complexity within a sector based on small samples. To exploit the classification information within each factor, multiple diverse factor subsets（FSSs） are generated under guidance from factor noise and independence analysis. Then, a base complexity evaluator is built corresponding to each FSS. The final complexity evaluation result is obtained by integrating all results from the base evaluators. Experimental studies using real-world air traffic operation data demonstrate the advantages of our model for small-sample-based traffic complexity evaluation over other stateof-the-art methods.