Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were as...Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were assigned to two groups,80 in Group A and 70 in Group B.They were treated,respectively,with conventional therapy only or conventional therapy combined with CSI.Their platelet parameters,including blood platelet count(BPC),mean platelet volume(MPV),platelet distribution width(PDW) and plateletcrit(...展开更多
Objective: To explore the effect of composite Salviae droping pill(CSDP) on endothelin (ET-1) gene expression in circulating endothelial cells. Mothods: Seventy cases of stable angina pectoris were randomly divided in...Objective: To explore the effect of composite Salviae droping pill(CSDP) on endothelin (ET-1) gene expression in circulating endothelial cells. Mothods: Seventy cases of stable angina pectoris were randomly divided into two groups, the CSDP group and the isosorbide dinitrate (ID) group. They were treated with CSDP and ID respectively,their ET-1 gene expression in endothelial cells of peripheral circulation was measured before and after treatment by reverse transcriptase-polymerase chain reaction(RT-PCR) and compared between the two groups as well as with that of healthy subjects. Results: Electrophoresis banding of 546 bp cDNA procured from 59 cases of 70 patients was positive, while no positive banding was obtained from the healthy subjects. Six cases from the 29 patients treated with CSDP had their banding turned to negative,while in the ID group,no one turned to negative after treatment. And ET-1 PCR product (absorbed optic density) in the CSDP group was markedly lower than that in the ID group, P＜0.05. Conclusion: DSP could directly inhibit ET-1 gene expression in endothelial cells of peripheral circulation.展开更多
Objective: To explore the effect of Composite Salviae miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells. Methods:Semisolid agar culture and flow cytometry assay were performed for studying the chang...Objective: To explore the effect of Composite Salviae miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells. Methods:Semisolid agar culture and flow cytometry assay were performed for studying the change in radiosensitivity of HL60 cell line and fresh human leukemia cells after exposing to CSMI.Results: The D0 (the inverse of the slope of the survival curve) and SF2 (survival fraction at 2 Gy) of HL60 cell line were decreased from 1. 53, 0. 34 to 0. 93, 0. 12, respectively and apopotosis rates after radiation were raised significantly by CSMI. Furthermore, the concentration of CSMI and the time of mixed culture with CSMI before irradiation had positive relation to the effects mentioned above. Compared with control group, CSMI could increase the radiation-induced apoptosis of fresh leukemia cells (5. 89 ± 2. 91 vs 12. 05 ± 3. 06). Conclusion: CSMI could obviously enhance the radiosensitivity of leukemia cells.Original article on CJIM(Chin) 1998; 18(5): 279展开更多
文摘Objective:To explore the effect of composite salvia injection(CSI) on platelet parameters in children with anaphylactoid purpura(AP) and its clinical significance.Methods:One hundred and fifty children with AP were assigned to two groups,80 in Group A and 70 in Group B.They were treated,respectively,with conventional therapy only or conventional therapy combined with CSI.Their platelet parameters,including blood platelet count(BPC),mean platelet volume(MPV),platelet distribution width(PDW) and plateletcrit(...
文摘Objective: To explore the effect of composite Salviae droping pill(CSDP) on endothelin (ET-1) gene expression in circulating endothelial cells. Mothods: Seventy cases of stable angina pectoris were randomly divided into two groups, the CSDP group and the isosorbide dinitrate (ID) group. They were treated with CSDP and ID respectively,their ET-1 gene expression in endothelial cells of peripheral circulation was measured before and after treatment by reverse transcriptase-polymerase chain reaction(RT-PCR) and compared between the two groups as well as with that of healthy subjects. Results: Electrophoresis banding of 546 bp cDNA procured from 59 cases of 70 patients was positive, while no positive banding was obtained from the healthy subjects. Six cases from the 29 patients treated with CSDP had their banding turned to negative,while in the ID group,no one turned to negative after treatment. And ET-1 PCR product (absorbed optic density) in the CSDP group was markedly lower than that in the ID group, P＜0.05. Conclusion: DSP could directly inhibit ET-1 gene expression in endothelial cells of peripheral circulation.
文摘Objective: To explore the effect of Composite Salviae miltiorrhizae Injection (CSMI) on radiosensitivity of leukemia cells. Methods:Semisolid agar culture and flow cytometry assay were performed for studying the change in radiosensitivity of HL60 cell line and fresh human leukemia cells after exposing to CSMI.Results: The D0 (the inverse of the slope of the survival curve) and SF2 (survival fraction at 2 Gy) of HL60 cell line were decreased from 1. 53, 0. 34 to 0. 93, 0. 12, respectively and apopotosis rates after radiation were raised significantly by CSMI. Furthermore, the concentration of CSMI and the time of mixed culture with CSMI before irradiation had positive relation to the effects mentioned above. Compared with control group, CSMI could increase the radiation-induced apoptosis of fresh leukemia cells (5. 89 ± 2. 91 vs 12. 05 ± 3. 06). Conclusion: CSMI could obviously enhance the radiosensitivity of leukemia cells.Original article on CJIM(Chin) 1998; 18(5): 279
