After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after cul...After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.展开更多
[ Objective ] This study was conducted to improve quality standard of Compound Danshen tablets. [ Method] Tanshinones in Compound Danshen tab- lets were determined by HPLC method. [ Result] A good linear relationship ...[ Objective ] This study was conducted to improve quality standard of Compound Danshen tablets. [ Method] Tanshinones in Compound Danshen tab- lets were determined by HPLC method. [ Result] A good linear relationship was found in the range of 0.10 -0.50 μg, and the average recovery rate was 100.59% （RSD = 1.38% ）. [ Conclusion] The method is simple, rapid and reproducible, and could be used as a method for quality control of Compound Danshen Tablets.展开更多
目的考察微晶纤维素、交联聚维酮及微粉硅胶等新型辅料的应用对复方丹参片药剂学性质的影响。方法以片剂硬度、崩解时间和可压性为指标,比较不同的填充剂、崩解剂对复方丹参片药剂学性质的影响;并对采用新辅料制备的片剂与原薄膜衣片中...目的考察微晶纤维素、交联聚维酮及微粉硅胶等新型辅料的应用对复方丹参片药剂学性质的影响。方法以片剂硬度、崩解时间和可压性为指标,比较不同的填充剂、崩解剂对复方丹参片药剂学性质的影响;并对采用新辅料制备的片剂与原薄膜衣片中丹参酮ⅡA的溶出度进行比较。结果应用新辅料可显著加速复方丹参片的崩解和丹参酮ⅡA的溶出速率。结论新辅料可以改善复方丹参片的崩解和丹参酮ⅡA的溶出,对加快中药制剂(traditional Chinese medicine,TCM)的起效时间可能起到一定的促进作用,但对其生物药剂学性质和疗效的影响仍待进一步研究。展开更多
目的:建立复方丹参片中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法,并采用PCA和HCA法进行分析。方法:采用高效液相色谱法,采用AlltimaTM C_(18)(4.6mm×250mm,5μm)Serial No 209050188色谱柱;流动相:乙腈-水...目的:建立复方丹参片中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法,并采用PCA和HCA法进行分析。方法:采用高效液相色谱法,采用AlltimaTM C_(18)(4.6mm×250mm,5μm)Serial No 209050188色谱柱;流动相:乙腈-水;梯度洗脱;检测波长203nm;柱温:30℃;流速:1mL/min;进样量:5μL。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的线性范围分别为0.106～1.06μg(r=0.9994),0.533～5.33μg(r=0.9998),0.0524～0.524μg(r=0.9997),0.582～5.82μg(r=0.9999);平均加样回收率(n=9)分别为98.02%,98.29%,98.72%,99.77%,RSD分别为2.82%,3.59%,2.43%,2.95%。结论:PCA和HCA较全面的反映了复方丹参片样本的信息,评价结果具有一定的客观性,可为复方丹参片的质量控制提供参考。展开更多
基金supported by the Bureau of Traditional Chinese Medicine of Guangdong Province, No. 2010463the National Science and Technology"12~(th) Five-years"Major Special-purpose Foundation,No.2011ZX09201-201-01
文摘After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer＇s disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer＇s disease, with similar effects to donepezil.
文摘[ Objective ] This study was conducted to improve quality standard of Compound Danshen tablets. [ Method] Tanshinones in Compound Danshen tab- lets were determined by HPLC method. [ Result] A good linear relationship was found in the range of 0.10 -0.50 μg, and the average recovery rate was 100.59% （RSD = 1.38% ）. [ Conclusion] The method is simple, rapid and reproducible, and could be used as a method for quality control of Compound Danshen Tablets.
文摘目的考察微晶纤维素、交联聚维酮及微粉硅胶等新型辅料的应用对复方丹参片药剂学性质的影响。方法以片剂硬度、崩解时间和可压性为指标,比较不同的填充剂、崩解剂对复方丹参片药剂学性质的影响;并对采用新辅料制备的片剂与原薄膜衣片中丹参酮ⅡA的溶出度进行比较。结果应用新辅料可显著加速复方丹参片的崩解和丹参酮ⅡA的溶出速率。结论新辅料可以改善复方丹参片的崩解和丹参酮ⅡA的溶出,对加快中药制剂(traditional Chinese medicine,TCM)的起效时间可能起到一定的促进作用,但对其生物药剂学性质和疗效的影响仍待进一步研究。
文摘目的:建立复方丹参片中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法,并采用PCA和HCA法进行分析。方法:采用高效液相色谱法,采用AlltimaTM C_(18)(4.6mm×250mm,5μm)Serial No 209050188色谱柱;流动相:乙腈-水;梯度洗脱;检测波长203nm;柱温:30℃;流速:1mL/min;进样量:5μL。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的线性范围分别为0.106～1.06μg(r=0.9994),0.533～5.33μg(r=0.9998),0.0524～0.524μg(r=0.9997),0.582～5.82μg(r=0.9999);平均加样回收率(n=9)分别为98.02%,98.29%,98.72%,99.77%,RSD分别为2.82%,3.59%,2.43%,2.95%。结论:PCA和HCA较全面的反映了复方丹参片样本的信息,评价结果具有一定的客观性,可为复方丹参片的质量控制提供参考。
