In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous b...In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous body and/or intragastric injection of 4 g/kg compound light granules containing Radix Angelicae Sinensis and Raidix Paeoniae Alba at 4 days after modeling, once per day for 30 consecutive days. After administration, the animals were sacrificed and the intraorbital optic nerve was harvested. Hematoxylin-eosin staining revealed that the injured optic nerve was thinner and optic nerve fibers were irregular. After treatment with recombinant human ciliary neurotrophic factor, the arrangement of optic nerve fibers was disordered but they were not markedly thinner. After treatment with compound light granules, the arrangement of optic nerve fibers was slightly disordered and their structure was intact. After combined treatment with recombinant human ciliary neurotrophic factor and compound light granules, the arrangement of optic nerve fibers was slightly disordered and the degree of injury was less than after either treatment alone. Results of tensile mechanical testing of the optic nerve showed that the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly lower than the normal optic nerve. After treatment with recombinant human ciliary neurotrophic factor and/or compound light granules, the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly increased, especially after the combined treatment. These experimental findings indicate that compound light granules and ciliary neurotrophic factor can alleviate optic nerve injury at the histological and biochemical levels, and the combined treatment is more effective than either treatment alone.展开更多
In this article, we consider a class of compound vector-valued problem on upper-half plane C＋, which consists of vector Riemann problem along a closed contour in C＋ with matrix coefficient in H61der class and vector...In this article, we consider a class of compound vector-valued problem on upper-half plane C＋, which consists of vector Riemann problem along a closed contour in C＋ with matrix coefficient in H61der class and vector Hilbert problem on the real axis with essential bounded measurable matrix coefficient. Under appropriate assumption we obtain its solution by use of Corona theorem and factorization of matrix functions in decomposed Banach algebras.展开更多
文摘In this study, rabbit models of optic nerve injury were reproduced by the clamp method. After modeling, rabbit models were given one injection of 50 ng recombinant human ciliary neurotrophic factor into the vitreous body and/or intragastric injection of 4 g/kg compound light granules containing Radix Angelicae Sinensis and Raidix Paeoniae Alba at 4 days after modeling, once per day for 30 consecutive days. After administration, the animals were sacrificed and the intraorbital optic nerve was harvested. Hematoxylin-eosin staining revealed that the injured optic nerve was thinner and optic nerve fibers were irregular. After treatment with recombinant human ciliary neurotrophic factor, the arrangement of optic nerve fibers was disordered but they were not markedly thinner. After treatment with compound light granules, the arrangement of optic nerve fibers was slightly disordered and their structure was intact. After combined treatment with recombinant human ciliary neurotrophic factor and compound light granules, the arrangement of optic nerve fibers was slightly disordered and the degree of injury was less than after either treatment alone. Results of tensile mechanical testing of the optic nerve showed that the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly lower than the normal optic nerve. After treatment with recombinant human ciliary neurotrophic factor and/or compound light granules, the tensile elastic limit strain, elastic limit stress, maximum stress and maximum strain of the injured optic nerve were significantly increased, especially after the combined treatment. These experimental findings indicate that compound light granules and ciliary neurotrophic factor can alleviate optic nerve injury at the histological and biochemical levels, and the combined treatment is more effective than either treatment alone.
基金supported by the National Natural Science Foundation of China(10471107)RFDP of Higher Education(20060486001)
文摘In this article, we consider a class of compound vector-valued problem on upper-half plane C＋, which consists of vector Riemann problem along a closed contour in C＋ with matrix coefficient in H61der class and vector Hilbert problem on the real axis with essential bounded measurable matrix coefficient. Under appropriate assumption we obtain its solution by use of Corona theorem and factorization of matrix functions in decomposed Banach algebras.
文摘目的研究中药复方颈椎康复丸治疗蛋白聚糖诱导小鼠骨关节炎的影响,为扩大该药的适应证及临床应用提供参考。方法采用Balb/c小鼠进行实验,将小鼠随机分为正常组,模型组,颈椎康复丸高、低剂量(6、3 g·kg^(-1)·d^(-1))组。全部小鼠(正常组除外)腹腔注射0.2 mL DDA佐剂(含100μg蛋白聚糖)造模;正常组给予0.2 mL生理盐水。首次免疫后,每隔2周进行关节炎评估和体重监测直到第14周。应用HE染色和番红固绿染色观察关节组织病理形态。应用ELISA法测定小鼠血清炎症因子IL-1β、IL-6、TNF-α和IL-17A。结果颈椎康复丸对小鼠骨关节炎有明显的抑制作用,能降低关节炎性评分和肿胀度。镜下观察,颈椎康复丸能保护小鼠关节组织,减少炎性浸润。颈椎康复丸能降低小鼠血清中炎症因子IL-1β、IL-6、TNF-α和IL-17A水平。结论颈椎康复丸对蛋白聚糖诱导的小鼠骨关节炎有改善作用。
