Selection of Effective Bcl-2 Antisense Oligodeoxynucleotides with Computer Software and Experimental Assay

Objective： To explore and investigate the selection of effective antisense oligodeoxynuleotides with the help of computer and RNAstructure folding software. Methods： Bcl-2 gene was used as the target gene and five antisense oligodeoxynuleotides were designed to be bound to Bcl-2 mRNA optimal secondary structure regions that were predicted free from intramolecular fold or instability of free energy. The five antisense oligodeoxynucleotides were studied with experimental assay of leukemia cells, including cell grow assay with tropan blue exclusion, expression of Bcl-2 protein detected with immunochemistry and flowcytometry, Bcl-2 mRNA content detected with RT-PCR technique, as well as apoptosis observed and determined with morphonological method, electrophoresis and flowcytometry. Results： The results showed that two of the five antisense oligodeoxynucleotides were effective antisense oligodeoxynucleotides, which were able to inhibit cell growth in leukemia, to decrease the level of Bcl-2 mRNA and protein, to induce apoptosis of leukemia cells significantly. Conclusion： The computational prediction of antisense efficacy is faster than other methods and more efficient, which can potentially speed the development of sequences for both research and clinical applications.

Paleocontinental Reconstruction:Methodology,Computer Software and an Example of Application

Paleocontinental reconstruction is of the nature of multidisciplinary analysis . This paper discusses the significance of some more important disciplines , including paleomagnetism , paleoclimatology and paleobiogeography. Top attention should be paid to the synthetic analysis and geological explanation of geophysical data . Reassembling of the paleocontinents by geometric similarities is preliminary, geological considerations should receive more attention . Several rules constraining the reconstruction are proposed and Eider poles used to reassemble some separated Precambrian median massifs to their mother continents are given . A computer software for paleocontinental reconstruction , GUDALU , is introduced . The software is flexible in that it gives the user maximum freedom to demonstrate his or her geological opinions by providing means of organizing geographic data and rotational parameters ,many kinds of map projection of different features and dynamic editing of the reconstruction maps . Finally, an example of Mesoproterozoic reconstruction is demonstrated .

Teaching Reform on Team-based Innovative Talent Training Model in Computer Software

As a prominent branch of computer science,computer software focuses on technology development and practical application,and it is of great importance in modern social and economic life.Universities need to train postgraduate students that master software engineering to satisfy the needs of high-level talents,preparing for the rapid development of the software industry.Postgraduate is an important stage to cultivate talented students in software engineering.Mentors in relevant majors need to combine advantages of their team while establishing an innovative mode for training talents,mining most potentials of students,and cultivating their ability.We have explored the team-based training mode of computer software major and scientific evaluation methods of innovation ability,shared critical importance in fulfilling fruitions of research,and proposed solutions to real problems especially under the background of“Double First-Class”.This paper decomposes and quantifies the evaluation indicators of the innovation ability of computer software graduate students through literature research and questionnaire surveys.Consequently,we establish a talent evaluation indexing system and build a team-based evaluation system for the innovation ability of graduate student in computer software major.

Computer Aided Software Engineering(CASE)for CAD Systems Production

In the paper the basic elements of CAD systems from software point of view are dis- cussed.The creation and next the modification of CAD systems needs specialized software tools.In the paper the idea computer Aided Software Engineering(CASE)for CAD systems production and practi- cal software realization of such a software tool is considered.The main element of this tool is special program provided for convenient storage and modification of CAD source program.In the paper the simple examples are presented and discussed.

Research on the Computer Software Testing Method Based on Multiple Platforms

Informationization plays an important role in modern life and production.And various software is one of the bases for it.Before it goes into service,software needs to go through many steps,including software development,design,etc.In software development,test is the key to identify and control bugs and errors in the software.Therefore,software companies often test the software to ensure that it is qualified.In recent years,more attention has been paid to a multi-platform computer software testing method,which can make up for defects in traditional testing methods to improve test accuracy.Firstly,this paper illustrates the connotation and features of software testing.Secondly,common software testing platforms and their requirements are analyzed.Finally,this paper proposes software testing method based on multiple platforms.

DEVELOPMENT OF COMPUTER SIMULATION SOFTWAREON PHASE TRANSFORMATION DURING QUENCHING

A computer software to simulate the phase transformation during quenching is designed based on Avrami equation and Scheil additivity principle of incubation period. The isothermal transformation diagrams of supercooled austenite are described by cubic spline functions. This software is possess of a good graphic interface of Windows style, can simulate the whole process in austenite decomposition during continuous cooling. If the cooling rate was given, the fraction of various microstructures transformed of austenite decomposition during continuous cooling at any temperature can be calculated. The simulation results are checked with the quenching experiment of 45 steel. The results indicate that the simulation results are comparatively close to the experimental results.

Application of Software Restoration System in Computer Room Management

The computer laboratory of colleges and universities plays a key role in the scientific research and talent training of colleges and universities.The maintenance and management of computer room is relatively heavy.In recent years,the computer motherboard supports the development of restore function and network transmission function,which brings great convenience to the management and maintenance of computer room in colleges and universities.Among them,the representative is Tsinghua Tongfang's network with the transmission technology and Lenovo's network transmission technology,but in order to achieve the network with the transmission of this function,also need to meet a lot of conditions.This paper will mainly explore the management of college computer room cannot use the hardware network to restore the computer function.

Toward Developing a Syllabus-Oriented Computer-Based Question-Banks Software to Support Partially Computerized Exams

Aims: This study aims at designing and implementing syllabus-oriented question-bank system that is capable of producing paper-based exams with multiple forms along with answer keys. The developed software tool is named Χ(Chi)-Pro Milestone and supports four types of questions, namely: Multiple-choice, True/False, Short-Answer and Free-Response Essay questions. The study is motivated by the fact that student number in schools and universities is continuously growing at high, non-linear, and uncontrolled rates. This growth, however, is not accompanied by an equivalent growth of educational resources (mainly: instructors, classrooms, and labs). A direct result of this situation is having relatively large number of students in each classroom. It is observed that providing and using online-examining systems could be intractable and expensive. As an alternative, paper-based exams can be used. One main issue is that manually produced paper-based exams are of low quality because of some human factors such as instability and relatively narrow range of topics [1]. Further, it is observed that instructors usually need to spend a lot of time and energy in composing paper-based exams with multiple forms. Therefore, the use of computers for automatic production of paper-based exams from question banks is becoming more and more important. Methodology: The design and evaluation of X-Pro Milestone are done by considering a basic set of design principles that are based on a list of identified Functional and Non-Functional Requirements. Deriving those requirements is made possible by developing X-Pro Milestone using the Iterative and Incremental model from software engineering domain. Results: We demonstrate that X-Pro Milestone has a number of excellent characteristics compared to the exam-preparation and question banks tools available in market. Some of these characteristics are: ease of use and operation, user-friendly interface and good usability, high security and protection of the question bank-items, high stability, and reliability. Further, X-Pro Milestone makes initiating, maintaining and archiving Question-Banks and produced exams possible. Putting X-Pro Milestone into real use has showed that X-Pro Milestone is easy to be learned and effectively used. We demonstrate that X-Pro Milestone is a cost-effective alternative to online examining systems with more and richer features and with low infrastructure requirements.

Comparison of Measured Trough Values after Deriving the Initial Dose Setting of Vancomycin with a Conventional Computer Software and a Nomogram Based on the Revised Japanese 2016 Therapeutic Drug Monitoring Guidelines for Antimicrobial Agents

Background: Due to the relatively high renal toxicity of vancomycin injection (VCM), setting an initial dose that achieves a trough that ranges between 10 and 20 μg/mL on day 3 is important to ensure safety and minimize side-effects, especially for patients with low renal function. To address these issues, the revised 2016 Therapeutic Drug Monitoring (TDM) Guidelines for Antimicrobial Agents (GL2016) proposed the use of a renal function-based, estimate glomerular filtration rate (eGFR) nomogram for setting the dose of VCM in Japan. Methods: Our hospital introduced the use of the GL2016 in September 2016 for the patients administered VCM. After setting the initial VCM dose using 1) a conventional VCM analysis software and 2) the GL2016 eGFR nomogram, the measured trough values on day 3 were compared and evaluated in this study. Results: With the VCM analysis software, the mean measured trough value in the a-total group (n = 53) was 12.8 ± 4.7 μg/mL. With the eGFR nomogram, the mean measured trough value in the b-total group (n = 13) was 9.6 ± 4.6 μg/mL. However, when the different severities of renal function were compared, the mean measured trough value was more significantly lower in the b-1 group than in the a-1 group among subjects with G2 and above (eGFR ≥ 60 mL/min/1.73 m2), but it was similar between the a-2 group and the b-2 group among subjects with G3 and below (eGFR 60 mL/min/1.73 m2). The proportion of subjects reaching the various trough ranges shows similar tendency. Conclusions: These data suggested that the measured trough value on day 3 was generally lower when the initial dose was established using the eGFR nomogram based on the GL2016, and this was especially prominent among patients with normal renal function. As for subjects with low renal function, the trough values were relatively high while ensuring safety.

Research on Modeling and Reusing of Computer Numerical Control Software with Object-oriented Technology

To improve the reusable and configurable ability of computer numerical control ( CNC ) software, a new method to construct reusable model of CNC software with object-oriented (OO) technology is proposed. Based on analyzing function of CNC software, the article presents how to construct a general class library of CNC software with OO technology. Most function modules of CNC software can he reused because of inheritable capability of classes. Besides, the article analyzes the object relational model in request/report mode, and multitask concurrent management model, which can he applied on double-CPU hardware platform and Windows 95/NT environment. Finally, the method has been successfully applied on a turning CNC system and a milling CNC system, and some function modules have been reused.

Windows and Fieldbus Based Software Computer Numerical Control System

Computer numerical control(CNC)system is the base of modern digital and intelligent manufacturing technolo- gy.And opened its architecture and constituted based on PC and Windows operating system(OS)is the main trend of CNC sys- tem.However,even if the highest system priority is used in user mode,real-time capability of Windows(2000,NT,XP)for applications is not guaranteed.By using a device driver,which is running in kernel mode,the real time performance of Windows can be enhanced greatly.The acknowledgment performance of Windows to peripheral interrupts was evaluated.Harmonized with an intelligent real-time serial communication bus(RTSB),strict real-time performance can be achieved in Windows platform. An opened architecture software CNC system which is hardware independence is proposed based on PC and RTSB.A numerical control real time kernel(NCRTK),which is implemented as a device driver on Windows,is used to perform the NC tasks.Tasks are divided into real-time and non real-time.Real-time task is running in kernel mode and non real-time task is running in user mode.Data are exchanged between kernel and user mode by DMA and Windows Messages.

Computer Aided Call for Tenders: A Tool for Software Bidding

Under a broad comprehension of Software Engineering, it is preferred the term software life cycle instead of just software production. The reason is that cycle starts at software conception and stops when the software is relegated. Given contemporary companies’ market strategies of focusing on their competitive advantages, most of them have externalized their software production to outsourced services. Therefore, the call for software tenders has become a common step in the software life cycle. In this paper, we present a tool which aids non-experts to specify call for software tenders. The motivation was Chile situation of most of rural and semi-urban city halls which do not have engineering teams to build call for software tenders. We describe the problem in terms of lack of competitiveness and empty biddings generated by low quality calls for tenders. As a second step, we show the technical considerations to develop the proposed tool and its features. We include an initial tool perception from a first group of users.

Exploration on the Practice System of Computer Software Course under the New Engineering

The rapid development of information technology and the new economy is a challenge to the training of computer technology and engineering professionals. The cultivation of engineering talents in Computer Engineering under the new engineering is an important part of the construction and exploration of new engineering in universities. According to the teaching reform of computer specialty in the new engineering construction, starting from the practice of curriculum system of software, mainly discusses the basic ideas of practice teaching reform of the course of computer software, software engineering practice curriculum system and practice curriculum system implementation method. The basic ideas of teaching reform practice in the course of the software include: expanding computer professional students' knowledge, strengthening students' computer science and software engineering knowledge and engineering practice ability, strengthening the frontiers of computer science and technology guide. Software practice course system is divided into three levels, including basic language course, engineering basic professional practice course and the professional practice course of engineering application direction. The implementation method of engineering application practice curriculum system is discussed in detail, such as strengthening university-enterprise cooperation, introducing teachers of social training institutions and guiding students to participate in subject competition and innovation projects.

Development and Therapeutic Applications of Precise Gene Editing Technology

The advent of gene editing represents one of the most transformative breakthroughs in life science,making genome manipulation more accessible than ever before.While traditional CRISPR/Cas-based gene editing,which involves double-strand DNA breaks(DSBs),excels at gene disruption,it is less effective for accurate gene modification.The limitation arises because DSBs are primarily repaired via non-homologous end joining(NHEJ),which tends to introduce indels at the break site.While homology directed repair(HDR)can achieve precise editing when a donor DNA template is provided,the reliance on DSBs often results in unintended genome damage.HDR is restricted to specific cell cycle phases,limiting its application.Currently,gene editing has evolved to unprecedented levels of precision without relying on DSB and HDR.The development of innovative systems,such as base editing,prime editing,and CRISPR-associated transposases(CASTs),now allow for precise editing ranging from single nucleotides to large DNA fragments.Base editors(BEs)enable the direct conversion of one nucleotide to another,and prime editors(PEs)further expand gene editing capabilities by allowing for the insertion,deletion,or alteration of small DNA fragments.The CAST system,a recent innovation,allows for the precise insertion of large DNA fragments at specific genomic locations.In recent years,the optimization of these precise gene editing tools has led to significant improvements in editing efficiency,specificity,and versatility,with advancements such as the creation of base editors for nucleotide transversions,enhanced prime editing systems for more efficient and precise modifications,and refined CAST systems for targeted large DNA insertions,expanding the range of applications for these tools.Concurrently,these advances are complemented by significant improvements in in vivo delivery methods,which have paved the way for therapeutic application of precise gene editing tools.Effective delivery systems are critical for the success of gene therapies,and recent developments in both viral and non-viral vectors have improved the efficiency and safety of gene editing.For instance,adeno-associated viruses(AAVs)are widely used due to their high transfection efficiency and low immunogenicity,though challenges such as limited cargo capacity and potential for immune responses remain.Non-viral delivery systems,including lipid nanoparticles(LNPs),offer an alternative with lower immunogenicity and higher payload capacity,although their transfection efficiency can be lower.The therapeutic potential of these precise gene editing technologies is vast,particularly in treating genetic disorders.Preclinical studies have demonstrated the effectiveness of base editing in correcting genetic mutations responsible for diseases such as cardiomyopathy,liver disease,and hereditary hearing loss.These technologies promise to treat symptoms and potentially cure the underlying genetic causes of these conditions.Meanwhile,challenges remain,such as optimizing the safety and specificity of gene editing tools,improving delivery systems,and overcoming off-target effects,all of which are critical for their successful application in clinical settings.In summary,the continuous evolution of precise gene editing technologies,combined with advancements in delivery systems,is driving the field toward new therapeutic applications that can potentially transform the treatment of genetic disorders by targeting their root causes.

How Software Engineering Transforms Organizations: An Open and Qualitative Study on the Organizational Objectives and Motivations in Agile Transformations

Agile Transformations are challenging processes for organizations that look to extend the benefits of Agile philosophy and methods beyond software engineering.Despite the impact of these transformations on orga-nizations,they have not been extensively studied in academia.We conducted a study grounded in workshops and interviews with 99 participants from 30 organizations,including organizations undergoing transformations(“final organizations”)and companies supporting these processes(“consultants”).The study aims to understand the motivations,objectives,and factors driving and challenging these transformations.Over 700 responses were collected to the question and categorized into 32 objectives.The findings show that organizations primarily aim to achieve customer centricity and adaptability,both with 8%of the mentions.Other primary important objectives,with above 4%of mentions,include alignment of goals,lean delivery,sustainable processes,and a flatter,more team-based organizational structure.We also detect discrepancies in perspectives between the objectives identified by the two kinds of organizations and the existing agile literature and models.This misalignment highlights the need for practitioners to understand with the practical realities the organizations face.

Interactivity software tools for teaching in ophthalmology

The use of interactive audience software,such as audience response systems(ARS),in medical education has become increasingly popular in recent years.This technology allows instructors to engage students in real time,encouraging active participation and promoting effective learning.The benefits of interactive audience software in medical education include increased student engagement,promotion of active learning,and enhanced learning outcomes.However,there are also several challenges to its implementation,including technical difficulties,careful planning and preparation,over-reliance on technology,and ethical concerns related to privacy and data security.The cost of implementing interactive audience software may also be a barrier for some institutions.This paper specifically reviews six interactive software platforms,including Socrative,Quizizz,Pear Deck,Slido,Wooclap and ClassPoint.These platforms allow for real-time assessment of student understanding,feedback,and participation.They also enable instructors to adjust their teaching strategies based on student responses and feedback.Overall,interactive audience software has shown great potential to enhance learning and engagement in medical education.It is important for instructors to carefully consider the benefits and challenges of its implementation.While the cost of implementing interactive audience software may be a barrier for some institutions,there are free and low-cost options available.

Quafu-Qcover:Explore combinatorial optimization problems on cloud-based quantum computers

We introduce Quafu-Qcover,an open-source cloud-based software package developed for solving combinatorial optimization problems using quantum simulators and hardware backends.Quafu-Qcover provides a standardized and comprehensive workflow that utilizes the quantum approximate optimization algorithm(QAOA).It facilitates the automatic conversion of the original problem into a quadratic unconstrained binary optimization(QUBO)model and its corresponding Ising model,which can be subsequently transformed into a weight graph.The core of Qcover relies on a graph decomposition-based classical algorithm,which efficiently derives the optimal parameters for the shallow QAOA circuit.Quafu-Qcover incorporates a dedicated compiler capable of translating QAOA circuits into physical quantum circuits that can be executed on Quafu cloud quantum computers.Compared to a general-purpose compiler,our compiler demonstrates the ability to generate shorter circuit depths,while also exhibiting superior speed performance.Additionally,the Qcover compiler has the capability to dynamically create a library of qubits coupling substructures in real-time,utilizing the most recent calibration data from the superconducting quantum devices.This ensures that computational tasks can be assigned to connected physical qubits with the highest fidelity.The Quafu-Qcover allows us to retrieve quantum computing sampling results using a task ID at any time,enabling asynchronous processing.Moreover,it incorporates modules for results preprocessing and visualization,facilitating an intuitive display of solutions for combinatorial optimization problems.We hope that Quafu-Qcover can serve as an instructive illustration for how to explore application problems on the Quafu cloud quantum computers.

Recent advances in CRISPR-based genome editing technology and its applications in cardiovascular research

The rapid development of genome editing technology has brought major breakthroughs in the fields of life science and medicine. In recent years, the clustered regularly interspaced short palindromic repeats(CRISPR)-based genome editing toolbox has been greatly expanded, not only with emerging CRISPR-associated protein(Cas) nucleases, but also novel applications through combination with diverse effectors. Recently, transposon-associated programmable RNA-guided genome editing systems have been uncovered, adding myriads of potential new tools to the genome editing toolbox. CRISPR-based genome editing technology has also revolutionized cardiovascular research. Here we first summarize the advances involving newly identified Cas orthologs, engineered variants and novel genome editing systems, and then discuss the applications of the CRISPR-Cas systems in precise genome editing, such as base editing and prime editing. We also highlight recent progress in cardiovascular research using CRISPR-based genome editing technologies, including the generation of genetically modified in vitro and animal models of cardiovascular diseases(CVD) as well as the applications in treating different types of CVD. Finally, the current limitations and future prospects of genome editing technologies are discussed.