In the present work, bacterial soil communities of different grapevine exploitation samples are studied in order to elucidate the possible influence of different agrarian management techniques (use of fertilizers, ir...In the present work, bacterial soil communities of different grapevine exploitation samples are studied in order to elucidate the possible influence of different agrarian management techniques (use of fertilizers, irrigation with river water) may have on the rhizospheric microbiome of Vitis vinifera plants. Therefore, it is postulated the Cenophenoresistome as a novel methodology to evaluate complex communities' global resistance against different antibiotics, by using and adjusting a serial of techniques traditionally applied to evaluate a monospecific population's resistance against antibiotics (Vitek, ATB and disk diffusion methods). Likewise, the metabolic profile (CLPP: comunity level physiological profile) of bacterial communities is studied by Biolog ECO. In relation to the functional structure of the bacterial communities, it is observed that the metabolic profile (diversity, kinetics and CLPP) of unexploited soils differs from soils under anthropic influence. It is discussed the causes of resistance in the human clinic antibiotic treatment based on the agrarian management, especially with the contamination transmitted by irrigation water, which could be associated with changes in edaphic communities. The results obtained in the present study through two different approaches (Cenophenoresistome and metabolic profiles) are consistent with each other, suggesting that both methods can be good bioindicators of the state of humankind-altered soils that host natural ecosystems. Likewise, the concept of Cenophenoresistome is proposed as a bioindicator of soil response to alteration processes, as well as a possible predictor of its evolution in edaphic remediation processes.展开更多
The purpose of this paper was to find out whether the higher education has met the necessary requirements for the implementation of the ICT system, in particular the e-learning system in higher education of seafarers....The purpose of this paper was to find out whether the higher education has met the necessary requirements for the implementation of the ICT system, in particular the e-learning system in higher education of seafarers. The goal was to explore to what extent the technical and organisational prerequisites for introducing the system have been fulfilled at the very institution--E-learning system provider, and to what extent the full-time maritime students and part-time students-seafarers are ready and trained to adopt such a system. In order to gather insights and make conclusions, the analytical method and the survey of students were used. A total of 930 full-time and part-time students (seafarers) were surveyed. The goal of these surveys was to find out the educational needs of both student groups, as well as their technical preconditions (e.g., having a PC at work or at home, access to the intemet, and the like) and competencies (e.g., computer literacy) that are necessary for active involvement in the e-learning system. A particularly interesting part of the research tackled the psychological predisposition of students, i.e., whether they were willing to use the system at all. A special part of the research was focused on issues regarding the costs and the available time that the students/seafarers have for using the e-learning system. The research has revealed that there are neither obstacles nor initial constraints for the implementation of the e-learning system in education of maritime students and seafarers at the University of Split. Moreover, the students have a positive attitude towards the e-learning system, and most of them are very enthusiastic about its implementation. The scientific contribution of this paper is provided by an analytical model exploring and defining the necessary prerequisites for introducing the e-learning system. The advantage of the model lies in its wide and multi-disciplinary approach to all these prerequisites, ranging from psychological to purely technical, financial, time, organisational and other aspects. An interesting issue is the question of"good will" for launching such systems at the institutions which obviously meet all necessary prerequisites for their introduction, including the enthusiasm of their students and teachers. The case study shows that, apparently, the university management and the wider community have not recognised many a benefit of the e-learning system. This sounds paradoxical as it is precisely the very academic community that should act as the designer and the initiator of social changes, in social systems in general and in education systems in particular.展开更多
文摘In the present work, bacterial soil communities of different grapevine exploitation samples are studied in order to elucidate the possible influence of different agrarian management techniques (use of fertilizers, irrigation with river water) may have on the rhizospheric microbiome of Vitis vinifera plants. Therefore, it is postulated the Cenophenoresistome as a novel methodology to evaluate complex communities' global resistance against different antibiotics, by using and adjusting a serial of techniques traditionally applied to evaluate a monospecific population's resistance against antibiotics (Vitek, ATB and disk diffusion methods). Likewise, the metabolic profile (CLPP: comunity level physiological profile) of bacterial communities is studied by Biolog ECO. In relation to the functional structure of the bacterial communities, it is observed that the metabolic profile (diversity, kinetics and CLPP) of unexploited soils differs from soils under anthropic influence. It is discussed the causes of resistance in the human clinic antibiotic treatment based on the agrarian management, especially with the contamination transmitted by irrigation water, which could be associated with changes in edaphic communities. The results obtained in the present study through two different approaches (Cenophenoresistome and metabolic profiles) are consistent with each other, suggesting that both methods can be good bioindicators of the state of humankind-altered soils that host natural ecosystems. Likewise, the concept of Cenophenoresistome is proposed as a bioindicator of soil response to alteration processes, as well as a possible predictor of its evolution in edaphic remediation processes.
文摘The purpose of this paper was to find out whether the higher education has met the necessary requirements for the implementation of the ICT system, in particular the e-learning system in higher education of seafarers. The goal was to explore to what extent the technical and organisational prerequisites for introducing the system have been fulfilled at the very institution--E-learning system provider, and to what extent the full-time maritime students and part-time students-seafarers are ready and trained to adopt such a system. In order to gather insights and make conclusions, the analytical method and the survey of students were used. A total of 930 full-time and part-time students (seafarers) were surveyed. The goal of these surveys was to find out the educational needs of both student groups, as well as their technical preconditions (e.g., having a PC at work or at home, access to the intemet, and the like) and competencies (e.g., computer literacy) that are necessary for active involvement in the e-learning system. A particularly interesting part of the research tackled the psychological predisposition of students, i.e., whether they were willing to use the system at all. A special part of the research was focused on issues regarding the costs and the available time that the students/seafarers have for using the e-learning system. The research has revealed that there are neither obstacles nor initial constraints for the implementation of the e-learning system in education of maritime students and seafarers at the University of Split. Moreover, the students have a positive attitude towards the e-learning system, and most of them are very enthusiastic about its implementation. The scientific contribution of this paper is provided by an analytical model exploring and defining the necessary prerequisites for introducing the e-learning system. The advantage of the model lies in its wide and multi-disciplinary approach to all these prerequisites, ranging from psychological to purely technical, financial, time, organisational and other aspects. An interesting issue is the question of"good will" for launching such systems at the institutions which obviously meet all necessary prerequisites for their introduction, including the enthusiasm of their students and teachers. The case study shows that, apparently, the university management and the wider community have not recognised many a benefit of the e-learning system. This sounds paradoxical as it is precisely the very academic community that should act as the designer and the initiator of social changes, in social systems in general and in education systems in particular.