Summary: To establish the determination method of dauricine (Dan) concentration in rats' blood and other biological samples, a reverse-phase HPLC method was adopted. Under the given condition, dauricine could be ...Summary: To establish the determination method of dauricine (Dan) concentration in rats' blood and other biological samples, a reverse-phase HPLC method was adopted. Under the given condition, dauricine could be well separated. The retention time (tR) of Dan and its internal standard, daurisoline were 9. 2 and 6.1 respectively. The detection limit was 10-2 mg/ml. The absolute recoveries of all kinds of samples were above 70 %, and the relative ones were over 85%. A good liner relationship has been obtained over the entire range of 0. 030 to 3. 000 rug/L in blood samples and 0. 050 to 5. 000 mg/L in other tissue samples. The intraday and interday coefficients of varia- tion were below 10 %. The results showed that the method can be used for detecting Dan in all kinds of biological samples.展开更多
Relative tillering rate(RTR)increased linear-ly with the increasing of leaf N concentration(NLV)has been already reported.To testwhether this relationship could be used toquantitatively explain the difference in tille...Relative tillering rate(RTR)increased linear-ly with the increasing of leaf N concentration(NLV)has been already reported.To testwhether this relationship could be used toquantitatively explain the difference in tilleringamong a wide range of N application,field ex- periments were conducted at the IRRI farm,Los Banos,Laguna,the Philippines.Two in- dica cultivars,IR 72 and IR68284H wereused.For each cultivar,12 treatments includ- ing 4 N levels(0,60,120,and 180kgN·ha)and 3 transplanting spacing(30×20,20×20,and 10×20cm)were arranged in a ran-domized split-plot design with 4 replications.The N treatments were designated as mainplots and spacings as subplots.Fourteen-day-old seedlings were transplanted with 3seedlings per hill.The subplot area was 20m~2.Nitrogen fertilizer was applied as basal,atmidtillering,and at panicle initiation in threeequal splits.P,K,and Zn were applied asbasal at normal dosage.The field was flooded.Plant samples were taken every 7-14 d from 14d after transplanting to flower展开更多
文摘Summary: To establish the determination method of dauricine (Dan) concentration in rats' blood and other biological samples, a reverse-phase HPLC method was adopted. Under the given condition, dauricine could be well separated. The retention time (tR) of Dan and its internal standard, daurisoline were 9. 2 and 6.1 respectively. The detection limit was 10-2 mg/ml. The absolute recoveries of all kinds of samples were above 70 %, and the relative ones were over 85%. A good liner relationship has been obtained over the entire range of 0. 030 to 3. 000 rug/L in blood samples and 0. 050 to 5. 000 mg/L in other tissue samples. The intraday and interday coefficients of varia- tion were below 10 %. The results showed that the method can be used for detecting Dan in all kinds of biological samples.
文摘Relative tillering rate(RTR)increased linear-ly with the increasing of leaf N concentration(NLV)has been already reported.To testwhether this relationship could be used toquantitatively explain the difference in tilleringamong a wide range of N application,field ex- periments were conducted at the IRRI farm,Los Banos,Laguna,the Philippines.Two in- dica cultivars,IR 72 and IR68284H wereused.For each cultivar,12 treatments includ- ing 4 N levels(0,60,120,and 180kgN·ha)and 3 transplanting spacing(30×20,20×20,and 10×20cm)were arranged in a ran-domized split-plot design with 4 replications.The N treatments were designated as mainplots and spacings as subplots.Fourteen-day-old seedlings were transplanted with 3seedlings per hill.The subplot area was 20m~2.Nitrogen fertilizer was applied as basal,atmidtillering,and at panicle initiation in threeequal splits.P,K,and Zn were applied asbasal at normal dosage.The field was flooded.Plant samples were taken every 7-14 d from 14d after transplanting to flower
