Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CF...Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CFTRinh-172 or forskolin (FSK) in this study were used to treat human sperm separately, and the rates of sperm autophagy and progressive motility, mitochondrial membrane potential (MMP) and ATP concentration, and the expression levels of related factors were detected to explore their relationship. It was showed that sperms treated with CFTRinh-172 or FSK reduced the levels of cAMP, CFTR and PKA, but increased sperm autophagy rate, expression levels of AMPK and LC3B. However, reactive oxygen species content had no significant difference. It was indicated that low level of CFTR performed with cAMP and its downstream effectors such as PKA and AMPK to regulate mitochondrial structure and function, leading to increased autophagy rate and reduced vitality of sperm.展开更多
The audiometric zero level of air conduction for 100 healthy youths was determinedover a froequency range from 20 Hz to 10 KHz in 1971--1973 under the laboratory conditions.The results obtained have been further verif...The audiometric zero level of air conduction for 100 healthy youths was determinedover a froequency range from 20 Hz to 10 KHz in 1971--1973 under the laboratory conditions.The results obtained have been further verified recently on a selected group of 12 persons.This paper also presents the transfer experiments from the original earphone TDH-39(MX-41/AR) to six other types of earphones. The deviation of our data thus obtained from therecommendation by ISO is discussed.展开更多
文摘Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CFTRinh-172 or forskolin (FSK) in this study were used to treat human sperm separately, and the rates of sperm autophagy and progressive motility, mitochondrial membrane potential (MMP) and ATP concentration, and the expression levels of related factors were detected to explore their relationship. It was showed that sperms treated with CFTRinh-172 or FSK reduced the levels of cAMP, CFTR and PKA, but increased sperm autophagy rate, expression levels of AMPK and LC3B. However, reactive oxygen species content had no significant difference. It was indicated that low level of CFTR performed with cAMP and its downstream effectors such as PKA and AMPK to regulate mitochondrial structure and function, leading to increased autophagy rate and reduced vitality of sperm.
文摘The audiometric zero level of air conduction for 100 healthy youths was determinedover a froequency range from 20 Hz to 10 KHz in 1971--1973 under the laboratory conditions.The results obtained have been further verified recently on a selected group of 12 persons.This paper also presents the transfer experiments from the original earphone TDH-39(MX-41/AR) to six other types of earphones. The deviation of our data thus obtained from therecommendation by ISO is discussed.
