The Condyloma Acuminatum’s Recurrence Can Be Reduced by Lesional Auto-Transplantation

Background: Condyloma acuminatum (CA), also known as a genital wart (GW), is a sexually transmitted disease caused by human papillomavirus (HPV). HPV lesions, recurrence tendency, and risk of malignant formation are primarily dependent on the person’s immunity level. GW recurrence is a major challenge in CA treatment. The aim of this study was to explore how lesional auto-transplantation (LT) can be used to treat CA and decrease its recurrence. Methods: We treated CA through the preparation and implantation of tissue from nine CA patients in our dermatology clinic. Transplantation of small pieces of HPV lesions to the subcutaneous fat of the inguinal region was carried out with the help of a simple surgical method under local anesthesia. Patients were followed up for six to eight months. We searched PubMed and the Web of Science for treatment options for CA to compare our treatment method recurrence rate with existing treatment options. Results: During three months of follow-up, seven patients experienced no recurrence of condyloma lesions, while two patients experienced recurrence of small lesions of condyloma acuminatum. The recurrence rate of CA once treated by auto-transplantation was relatively low compared to other CA treatment options. Conclusions: Transplantation of HPV lesions to the inguinal area reduces the recurrence rate in sexually active individuals. The 2/9 recurrence after implantation could be due to poor hygiene, sexual relationships or immune factors.

Unusual presentation of penile giant condyloma acuminatum with spontaneous prepuce perforation: A case report

BACKGROUND Giant condyloma acuminatum(GCA)is an uncommon condition affecting the anogenital area.CASE SUMMARY Here,we report an 88-year-old male patient who presented with a 5-year history of a progressive appearance of multiple cauliflower-like warts over his penile shaft,pubis region,and groin as well as urine leakage along the distal penile shaft.Physical examination revealed an ulcerative skin perforation with pus discharge under the distal prepuce base,which was initially suspected to be a urethral fistula.However,during surgery,it was discovered that the perforation was caused by a giant condyloma lesion that had obliterated the prepuce opening,with infection and high pressure causing subsequent skin perforation.He underwent circumcision and wide excision with electrocauterization of the warts.He was discharged after the surgery,and the residual lesion was treated with imiquimod and low-dose oral tegafur-uracil.CONCLUSION Penile GCA can cause prepuce perforation and can be postoperatively treated with imiquimod and low-dose oral tegafur-uracil.

Fas and Bc1-2 EXpression on T Lymphocyte Subsets in the Peripheral Blood of Relapsing Patients with Condyloma Acuminatum

Objective: To study the expression of Fas and Bcl-2proteins on T lymphocyte subsets in the peripheralblood of relapsing patients with condyloma acuminatum(CA) and healthy controls. Methods: Flow cytometry (permeabization andstaining procedure with conjugated antibodies) wasused. Results: We observed that the expression of Fasprotein on CD4^+ T lymphocyte subset of CA patientswas significantly higher than that of healthy controls(P<0.01). Conclusions: Increased expression of Fas proteinon CD4^+ T lymphocyte subset may be a cause of de-creased percentage of CD4^+ T lymphocyte subset. Thisinduces the increased ratio of CD4^+/CD8^+.

Observation of Ultrastructure in Lesions of Condyloma Acuminatum

Objective: To explore the effect of morphological changes in the development of condyloma acuminatum (CA) Materials and Methods: Lesions in five patients with CA were observed . Results: Upon electron microscopy, the most characteristic feature of the lesions important for diagnosis, was the presence of distinct perinuclear vacuolizations, or so-called koilocytes, among some epithelial cells. These cells possessed hyperchromatic nuclei, swollen mitochondria, dilated endoplasmic reticulum and dissolved glycogen. There were interchromatin granules and perichromatin granules in some nuclei. Moreover some virus particles were also seen in the nuclei of some infected cells. Conclusions: The ultrastructural findings may be used to histopathologically explain the pathogenesis and mechanism of this disease, and it is helpful for diagnosis of CA .

Effect of Activation-induced Cell Death of Peripheral Blood Mononuclear cells in Patients with Condyloma Acuminatum

Objective: To investigate the effect of activation-induced cell death (AICD) on cellular immune function in the condyloma acuminatum(CA). Methods: Peripheral blood mononuclear cells (PBMC) were isolated from normal healthy individuals (control group) and patients with CA. PBMC were cultured with PHA-P for 48h in vitro. Apoptosis of the PBMC was detected by flow cytometry. Supernatant cytokines (IL-2 and IL-10) were assayed by ELISA. Results: The rate of PBMC apoptosis in both CA group and control group in fresh PBMC was very low and similar in both groups(P>0.05). The rate of PBMC apoptosis within the CA group was noticeably increased compared to that of the control (P<0.001)af-ter PBMC were cultured for 48h. The level of IL-2 was significantly lower in the CA group than in the control group (P<0.001), The level of IL-10 was significantly higher in the CA group compared to thecontrol group(P<0.001). Conclusion: Study results indicate that AICD may affect cellular mediated immune function and play an important role in the pathogenesis of CA.

The Lymphoblastoid Cell Lines of Recurrence Condyloma Acuminatum Patients Produce Lower Level of Tumor Necrosis Factor Stimulated with LPS

To study the mechanism of Condyloma acuminatum (CA) recurrence, and the association of CA recurrence with the ability of the host derived lymphoblastoid cell lines (LCL) stimulated by LPS to produce tumor necrosis factor (TNF), EBV-transformed B LCL were used as TNF producing cells The ability of LCL stimulated by LPS to produce TNF was measured by bioassay The results showed that the LCL from CA patients (including recurrent and non-recurrent CA patients) produced similar level of TNF stimulated by LPS to that of normal controls (29 54%±11 28% vs 34 31%±11 46%, P =0 1498) The LCL of CA recurrent patients produced significantly lower amount of TNF than that of non-recurrent CA patients (23 72%±7 41% vs 37 33%±11 10%, P =0 0032) Compared with the normal controls, CA recurrent patients showed a decreased ability to produce TNF (23 72%±7 41 vs 34 31%±11 46, P =0 0054), whereas CA non recurrent patients had the similar ability to the controls (37 33%±11 10 vs 34 31%±11 46, P =0 4914) It was concluded that the onset of CA was not relevant to the individual's ability to produce TNF But the recurrence of CA was associated with the ability to produce TNF It was also indicated that the TNF involved cellular immunity might play an important role in the clearance of the residual HPV by the host after treatment

Telomerase Activity in Condyloma Acuminatum Tissue with Different HPV Types

Summary: The telomerse activity in condyloma acuminatum (CA) tissue with human papillomavirus (HPV) types of 6/11 and 16/18 was detected to investigate the function of telomerase in the occurrence, development and carcinogenesis of genital CA. Forty-two biopsies from patients with gennital CA and 30 control tissue samples were tested for telomerase activity, HPV presence and types. The telomerase activity was determined by modified telomerase repeat amplification protocol (TRAP) assay and HPV typing by polymerase chain reaction (PCR) with typing-specific primers. Results showed that HPV-DNA was negative and the expression rate of telomerase was 16.7 % in all normal skin samples. All CA samples were positive for HPV (6/11 type was found in 32 cases, 16/18 in 3 and mixed type in 7). Telomerase activity was detectable in all CA patients. The telomerase activity in CA of 16/18 type was apparently higher than in CA of 6/11 type. It was concluded that the hyperplasia in CA might be increased as a result of HPV infection, suggesting that the activation of telomerase by HPV, especially by 16/18 type may play a role in the etiology and carcinogenesis of genital CA.

Expression of CD40 and CD40L on Peripheral Blood Mononuclear Cells in Patients with Condyloma Acuminatum

To observe the expression of CD40/CD40L on peripheral blood mononuclear cells （PBMC） in patients with eondyloma aeuminatum （CA）, flow eytometry was employed to examine the expression of CD40 and CD40L on PMBC in 36 patients with CA and 20 healthy controls. Our results showed that mean level of CD40 expression in CA patients was significantly lower than that in the controls （6.58 %±2.74 % vs 14.81 %±6.12 %, t=5. 703, P〈0.05）; the average level of CD40L in CA patients was also significantly lower than that in the controls （0.73 % ±0.54 % vs 2.67 %±2.43 %, t=3. 532, P〈0.05）. Our resutls suggest that the reduced costimulatory interaction of CD40 and CD40L in CA patients may be one of the important factors responsible for the low cellular immunity.

Changes of Circulating Platelet-Derived Growth Factor and Granulocyte Colony-Stimulating Factor in Patients with Condyloma Acuminatum

Objective: To study the expression levels of platelet-derived growth factor (PDGF) and granulocyte colony-stimulatingfactor (G-CSF) in peripheral blood and their role in thepathogenesis of Condyloma acuminatum (CA). Methods: Sera were taken from 70 patients with Condylomaacuminatum and compared with 35 healthy controls. PDGFand G-CSF in serum were quantitated using a dual antibodysandwich enzyme-linked immunoabsorbent assay (ELISA). Results: Serum concentrations of PDGF and G-CSF weresignificantly increased in patients with Condylomaacuminatum (CA) compared to controls (P<0.001 and P<0.005respectively). Serum levels of PDGF and G-CSF correlatedwith clinical severity of CA, but no significant difference wasobserved between different duration of disease groups. Asignificant positive correlation was noticed between neutrophilcount and G-CSF levels (γ=0.38, P<0.001), and the neutrophilcount showed no significant correlation with PDGE Conclusion: The results indicated that increased expressionof PDGF an -CSF in peripheral blood might be involved in pathogenesis of CA.

Perianal Giant Condyloma Acuminatum:A Case Report

Rare giant condyloma acuminatum （CA） reported by this paper is an interesting unusual case in China. Giant CA is a tumor that primarily affects the genital and perianal areas. Its feature is the high rate of local recurrence and transformation into squamous cell carcinoma. Making choice of wide surgical excision and using interferon as antiviral and immunoenhancement for CA after operation, we obtain satisfactory functional and cosmetic results.

CO_(2)LASER PLUS PHOTODYNAMIC THERAPY VERSUS CO_(2)LASER IN THE TREATMENT OF CONDYLOMA ACUMINATUM:A RANDOMIZED COMPARATIVE STUDY

To compare the efficacy and safety of CO_(2)laser plus 5-aminolevulinic acid(ALA)photodynamic therapy(PDT)with CO_(2)laser for the treatment of multiple condyloma acuminatum(CA),120 patients with multiple CA were allocated into two groups—combined group(CO_(2)laser plus ALA-PDT,n=60)and CO_(2)laser group(CO_(2)laser plus placebo-PDT,n=60).After CO_(2)laser,a 20%ALA or a placebo solution was applied to the CA area 3 h before illumination with red light(635 nm,100mW/cm^(2),80 J/cm^(2)).The treatment was repeated seven days after the first treatment if the lesions were not completely resolved.The complete response rate,recurrence rate and adverse effects in the two groups were analyzed.After two treatments,the complete response rates in the CO_(2)laser group and combined group were 100%(509/509)and 100%(507/507)in the CA(p>0:05),respectively.The recurrence rates in the CO_(2)laser group and combined group were 44.9%(229/509)and 10.6%(54/507)in the CA(p<0:05),respectively.The adverse effects in CO_(2)laser group was more than that in combined group.The combined group is a more effective treatment for multiple CA compared with CO_(2)laser group.T/S.Style the highlighted text as abstract.

A comparative study on the angiogenic activity of condyloma acuminatum and basal cell carcinoma

Recently, the study on the extraction, component analysis and function of angiogenic factor (AF) has become an important subject in anti-tumor research and made a great progress. Therefore, the imitation of the tumor's angiogenic activity (AGA) possibly helps to find a new method to treat ischemic diseases by blood vessel proliferation. Most of the parenchymatous malignant tumors have AGA. We studied the early stage condyloma acuminatum (CA) . After transplanting CA onto the chorioallantoic membrane (CAM) of chick embryos, we observed the AGA of CA and carefully compared it with that of basal cell carcinoma which is generally considered to have strong AGA. Meanwhile, we explored the way by which the AF of CA comes into being. The results demonstrated that CA, a benign neoplastic lesion, at its early stage, also has AGA. It is postulated that the responsible material is a certain form of AF. Therefore, the study on AGA of benign lesions (such as CA) and its physical basis will have potential clinical significance.

A Correlative Analysis of Cervical Lesions in Patients with Vulva Condyloma Acuminatum

OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus （HPV） infections and cervical epithelium lesions. METHODS From May 2002 to April 2004 patients with vulva condyloma acuminatum were examined employing vulva biospy, colposcopy, highrisk HPV-DNA test and cervical bioscopy. RESUTS In 418 cases of vulva condyloma acuminatum, verified by pathologic analysis, high-risk HPV （＋） infections were detected in 68.7% （287/418） of the cases. Among those patients, 20,6% （59/287） had concurrent subclinical cervical intraepithelial neoplastic （CIN） lesions. Among the high-risk HPV（-） 31.3 % （131）, patients 7,6% （10/131） had concurrent subclinical CIN lesions, Pathological examination results： cervicitis, 167 （40.0%）; cervical HPV infection, 182 （43.5%）; CIN-Ⅰ, 51（12.2%）; CIN- Ⅱ, 16 （3.83%）; CIN-Ⅲ, 2 （0,5%）; cervical cancer, none. Another patient had vulva condyloma acuminatum with valva intraepithelial neoplasia （VIN） Ⅱ-Ⅲ, CONCLUSION It was concluded that simultaneous cervical HPV infection of many types,was rather common in patients with vulva condyloma acuminatum. Vulva condyloma acuminatum is the chief clinical symptom which hints at a high possibility of infection with high-risk HPV. The patients are at high-risk for CIN and cervical cancer. We must pay more attention to the cervix in cases with vulva condyloma acuminatum.

MICROSTRIP APPLICATOR FOR TREATMENT OF CERVICAL EROSION AND CERVICAL CONDYLOMA ACUMINATUM

This Paper introduces a microstrip applicator for treatment of cervical erosion and cervical condyloma acuminatum with microwave tissue coagulationmethod. The principle of the applicator,the theoretical calculation of near field distribution of the applicator covered with a lossy dielectric, the experimental resultsfor its performance of the impedance matching and for microwave leakage energyare reported. Finally,the therapeutic method and effects are presented.

尖锐湿疣患者恐惧疾病进展现状及影响因素

目的探讨尖锐湿疣患者恐惧疾病进展现状及相关影响因素。方法采用便利抽样法,选取从2021年12月至2023年1月在郑州大学第一附属医院确诊为尖锐湿疣的263例患者。采用患者一般资料调查表、中文版恐惧疾病进展简化量表、中文版痛苦表露指数量表、心理弹性量表简表等进行调查,使用SPSS 25.0中Spearman秩相关分析和多重线性回归分析影响尖锐湿疣患者恐惧疾病进展的相关因素。结果患者恐惧疾病进展得分为(38.21±5.64)分,心理弹性得分为[73.00(65.00,77.00)]分,自我表露得分为[35.00(31.00,39.00)]分,患者的心理弹性水平、自我表露水平、确诊时间、是否首次治疗是影响患者恐惧疾病进展水平的相关因素。结论关注病程较长的患者,在首次治疗时进行干预,促进患者自我表露,提高心理弹性水平,降低患者疾病进展的恐惧感。

男性尖锐湿疣患者组织人乳头瘤病毒基因型的感染情况

目的:分析男性尖锐湿疣患者组织人乳头瘤病毒(HPV)基因型的感染情况。方法:选择2021年12月—2023年12月于青岛市第三人民医院就诊的199例男性尖锐湿疣患者,采集疣体组织,采用聚合酶链式反应(PCR)-反向斑点杂交法检测HPV感染情况,统计男性尖锐湿疣患者HPV感染基因分型,不同感染状态、类型、年龄段患者HPV感染情况。结果:199例男性尖锐湿疣患者HPV感染基因分型中以6亚型和11亚型为主,分别占33.00%、19.19%。低危型、高危型、混合型HPV感染占比分别为53.77%、21.11%、25.13%,以低危型为主。单一感染、二重感染、多重感染占比分别为74.87%、11.56%、13.57%,以单一感染为主。<20岁、20~29岁、30~39岁、40~49岁、50~59岁、≥60岁患者的HPV感染占比分别为6.03%、33.67%、25.63%、19.60%、13.57%、1.51%,感染人群以20~29岁为主。结论:男性尖锐湿疣患者HPV感染基因分型以6亚型和11亚型为主,以低危型、单一感染居多,患病人群年龄集中于20~29岁,临床应加以重视,积极做好男性尖锐湿疣HPV感染防治工作。

尖锐湿疣与雌激素、孕激素相关性的研究进展

尖锐湿疣(CA)是一种以肛门生殖器部位增生性损害为主要表现的性传播疾病,其发病主要与HPV感染及机体免疫应答障碍相关。CA发病率女性高于男性,妊娠期高于非妊娠期,性激素可能与CA发生发展有关。本文就雌孕激素与尖锐湿疣的相关性作一综述,分析雌孕激素与尖锐湿疣在HPV感染及细胞免疫的Th1/Th2、Th17/Treg平衡等方面可能存在的联系,探讨雌孕激素在尖锐湿疣发病及复发中所起的作用,以期为尖锐湿疣的临床诊治提供新思路。

ATG3和ATG5在尖锐湿疣患者皮损组织中的表达及其临床意义

目的探讨尖锐湿疣患者皮损组织中自噬相关基因3(ATG3)与自噬相关基因5(ATG5)的表达水平及临床意义。方法选取青岛市城阳区人民医院收治的92例尖锐湿疣患者作为研究组。选取同期因其他原因切除外生殖器正常皮肤组织的63例健康者作为对照组,其中45例男性行包皮环切术,18例女性行外阴整形术。收集并记录所有研究对象的一般资料,检测皮损组织中ATG3和ATG5的表达水平,采用Pearson法分析ATG3和ATG5表达水平的相关性,采用受试者工作特征(ROC)曲线分析ATG3、ATG5表达水平预测尖锐湿疣复发的效能。结果研究组患者皮损组织中ATG3(1.28±0.27)和ATG5(0.91±0.23)表达水平较对照组(1.86±0.39,1.34±0.31)显著降低(P<0.05)。皮损组织ATG3和ATG5表达水平与疣体团块直径及人乳头瘤病毒(HPV)类型有关(P<0.05)。Pearson法相关分析显示,尖锐湿疣患者皮损组织中ATG3和ATG5表达水平呈正相关(r=0.527,P<0.05)。复发组皮损组织中ATG3和ATG5表达水平显著低于未复发组(P<0.05)。皮损组织中ATG3、ATG5单独及联合预测尖锐湿疣复发的AUC分别为0.729、0.785和0.907,二者联合优于ATG3、ATG5单一指标预测(Z_(ATG3-联合)=2.876、Z_(ATG5-联合)=2.064,P<0.05)。结论ATG3和ATG5在尖锐湿疣患者皮损组织中表达水平显著降低,二者联合可用于预测尖锐湿疣的复发,临床应用价值较大。

咪喹莫特乳膏联合光动力疗法治疗外阴尖锐湿疣患者的效果

目的:观察咪喹莫特乳膏联合光动力疗法治疗外阴尖锐湿疣患者的效果。方法:选取2021年8月至2023年8月该院收治的98例外阴尖锐湿疣患者进行前瞻性研究,按照随机数字表法将其分为对照组和研究组各49例。对照组采用光动力疗法治疗,研究组在对照组基础上联合咪喹莫特乳膏治疗,比较两组临床疗效、治疗前后炎性指标[肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、C反应蛋白(CRP)]水平、血清再生因子指标[血管内皮生长因子(VEGF)、缺氧诱导因子-1α(HIF-1α)]水平、不良反应发生率和复发率。结果:研究组治疗总有效率为95.92%(47/49),高于对照组的83.67%(41/49),差异有统计学意义(P<0.05);治疗后,两组TNF-α、CRP、IL-6、VEGF、HIF-1α水平均低于治疗前,且研究组低于对照组,差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05);随访3个月,研究组复发率为0,低于对照组的12.20%(5/41),差异有统计学意义(P<0.05)。结论:咪喹莫特乳膏联合光动力疗法治疗外阴尖锐湿疣患者可提高治疗总有效率,降低炎性指标水平、血清再生因子指标水平和复发率,效果优于单纯光动力疗法治疗。

点阵二氧化碳激光联合光动力治疗肛门尖锐湿疣复发因素分析

目的:通过回顾性分析点阵二氧化碳(carbon dioxide,CO_(2))激光联合5-氨基酮戊酸光动力疗法(5-aminolevulinic acid photodynamic therapy,ALA-PDT)治疗肛门尖锐湿疣(condyloma acuminatum,CA)患者的临床资料,以评估影响术后复发的因素。方法:收集2015年4月─2022年12月间在南通大学附属南通第三医院皮肤科接受点阵CO_(2)激光联合光动力治疗的肛门CA患者,分为复发组和治愈组,分析两组患者的临床资料,采用Cox回归模型对患者的临床特征进行单因素及多因素回归分析。结果:56例肛门CA患者纳入分析,其中复发8例,未复发48例,复发率为14.28%。单因素及多因素Cox回归分析表明,点阵CO_(2)激光次数(>1次)与肛门CA复发呈正相关[HR=13.0,95%CI(2.1,79.9),P=0.006],不合并基础疾病与复发呈负相关[HR=0.1,95%CI(0,0.4),P=0.003]。结论:CO_(2)激光次数及合并基础疾病是点阵CO_(2)激光联合光动力治疗肛门CA复发的独立影响因素。