Objective: To detect the activated expression oftelomerase in condyloma acuminata lesions in low-risk (6/11)and high-risk (16/18) human papilloma virus (HPV)infection and examine the role played by telomerase in theoc...Objective: To detect the activated expression oftelomerase in condyloma acuminata lesions in low-risk (6/11)and high-risk (16/18) human papilloma virus (HPV)infection and examine the role played by telomerase in theoccurrence, development and carcinomatous change ofcondyloma acuminata. Methods: Assaying the expression of telomerase and thetype of HPV in damaged skin of 42 CA patients and normalskin of 30 healthy control individuals through telomeraserepeat amplification protocol (TRAP) and polymerase chainreaction (PCR). Results: In all the normal skin controls, PCR for HPV wasnegative and only 16.7% of samples were positive for TLMAexpression; in CA lesions, HPV testing was positive in all (32cases were low-risk, 3 were high-risk, and 7 were of mixedtype) and all were positive for TLMA expression. Conclusion: TLMA may be activated by HPV infection,and in turn cause the hyperplasia of epidermal cells. It wasalso indicated that HPV, especially high risk types, canactivate TLMA. The activation of TLMA may play animportant role in abnormal hyperplasia and carcinomatouschanges in CA lesions.展开更多
目的检测尖锐湿疣(condyloma acuminate,CA)患者血清中辅助性T淋巴细胞(helper T cell,Th细胞)相关细胞因子的表达水平,探讨其在CA发病机制中可能的作用,揭示CA患者全身的免疫状态。方法分别采集40例CA患者和20名健康对照者外周血,采用...目的检测尖锐湿疣(condyloma acuminate,CA)患者血清中辅助性T淋巴细胞(helper T cell,Th细胞)相关细胞因子的表达水平,探讨其在CA发病机制中可能的作用,揭示CA患者全身的免疫状态。方法分别采集40例CA患者和20名健康对照者外周血,采用高通量抗体芯片技术检测CA患者及健康对照者外周血血清中Th细胞相关细胞因子表达水平。结果CA患者外周血血清中Th1型细胞因子干扰素(IFN)-γ、白细胞介素(IL)-2、肿瘤坏死因子(TNF)-α表达水平分别为(5.11±3.44)pg/ml、(4.44±2.53)pg/ml、(30.16±7.38)pg/ml,较健康对照组(9.5±3.77)pg/ml、(8.26±3.26)pg/ml、(37.06±12.16)pg/ml降低;Th2型细胞因子IL-4表达水平为(4.18±2.29)pg/ml,较健康对照组(2.07±1.10)pg/ml升高;Treg型细胞因子IL-10、转化生长因子(TGF)-β表达水平分别为(2.03±1.01)pg/ml、(904.67±317.91)pg/ml,较健康对照组(1.26±0.71)pg/ml、(648.96±180.34)pg/ml升高;Th17型细胞因子IL-17及IL-23表达水平分别为(1.39±0.23)pg/ml、(172.98±42.56)pg/ml,较健康对照组(1.62±0.44)pg/ml、(204.23±48.88)pg/ml降低;以上差异均有统计学意义(均P<0.05)。结论Th1/Th2、Th17/Treg型细胞因子交互作用失衡很可能是CA重要的免疫学发病机制之一。CA患者存在全身性的细胞免疫功能紊乱。展开更多
文摘Objective: To detect the activated expression oftelomerase in condyloma acuminata lesions in low-risk (6/11)and high-risk (16/18) human papilloma virus (HPV)infection and examine the role played by telomerase in theoccurrence, development and carcinomatous change ofcondyloma acuminata. Methods: Assaying the expression of telomerase and thetype of HPV in damaged skin of 42 CA patients and normalskin of 30 healthy control individuals through telomeraserepeat amplification protocol (TRAP) and polymerase chainreaction (PCR). Results: In all the normal skin controls, PCR for HPV wasnegative and only 16.7% of samples were positive for TLMAexpression; in CA lesions, HPV testing was positive in all (32cases were low-risk, 3 were high-risk, and 7 were of mixedtype) and all were positive for TLMA expression. Conclusion: TLMA may be activated by HPV infection,and in turn cause the hyperplasia of epidermal cells. It wasalso indicated that HPV, especially high risk types, canactivate TLMA. The activation of TLMA may play animportant role in abnormal hyperplasia and carcinomatouschanges in CA lesions.
文摘目的检测尖锐湿疣(condyloma acuminate,CA)患者血清中辅助性T淋巴细胞(helper T cell,Th细胞)相关细胞因子的表达水平,探讨其在CA发病机制中可能的作用,揭示CA患者全身的免疫状态。方法分别采集40例CA患者和20名健康对照者外周血,采用高通量抗体芯片技术检测CA患者及健康对照者外周血血清中Th细胞相关细胞因子表达水平。结果CA患者外周血血清中Th1型细胞因子干扰素(IFN)-γ、白细胞介素(IL)-2、肿瘤坏死因子(TNF)-α表达水平分别为(5.11±3.44)pg/ml、(4.44±2.53)pg/ml、(30.16±7.38)pg/ml,较健康对照组(9.5±3.77)pg/ml、(8.26±3.26)pg/ml、(37.06±12.16)pg/ml降低;Th2型细胞因子IL-4表达水平为(4.18±2.29)pg/ml,较健康对照组(2.07±1.10)pg/ml升高;Treg型细胞因子IL-10、转化生长因子(TGF)-β表达水平分别为(2.03±1.01)pg/ml、(904.67±317.91)pg/ml,较健康对照组(1.26±0.71)pg/ml、(648.96±180.34)pg/ml升高;Th17型细胞因子IL-17及IL-23表达水平分别为(1.39±0.23)pg/ml、(172.98±42.56)pg/ml,较健康对照组(1.62±0.44)pg/ml、(204.23±48.88)pg/ml降低;以上差异均有统计学意义(均P<0.05)。结论Th1/Th2、Th17/Treg型细胞因子交互作用失衡很可能是CA重要的免疫学发病机制之一。CA患者存在全身性的细胞免疫功能紊乱。
