Summary: The telomerse activity in condyloma acuminatum (CA) tissue with human papillomavirus (HPV) types of 6/11 and 16/18 was detected to investigate the function of telomerase in the occurrence, development and car...Summary: The telomerse activity in condyloma acuminatum (CA) tissue with human papillomavirus (HPV) types of 6/11 and 16/18 was detected to investigate the function of telomerase in the occurrence, development and carcinogenesis of genital CA. Forty-two biopsies from patients with gennital CA and 30 control tissue samples were tested for telomerase activity, HPV presence and types. The telomerase activity was determined by modified telomerase repeat amplification protocol (TRAP) assay and HPV typing by polymerase chain reaction (PCR) with typing-specific primers. Results showed that HPV-DNA was negative and the expression rate of telomerase was 16.7 % in all normal skin samples. All CA samples were positive for HPV (6/11 type was found in 32 cases, 16/18 in 3 and mixed type in 7). Telomerase activity was detectable in all CA patients. The telomerase activity in CA of 16/18 type was apparently higher than in CA of 6/11 type. It was concluded that the hyperplasia in CA might be increased as a result of HPV infection, suggesting that the activation of telomerase by HPV, especially by 16/18 type may play a role in the etiology and carcinogenesis of genital CA.展开更多
OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus (HPV) infections and cervical epithelium lesions. METHODS From May 2002 to April 2004 patients with vulva condylo...OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus (HPV) infections and cervical epithelium lesions. METHODS From May 2002 to April 2004 patients with vulva condyloma acuminatum were examined employing vulva biospy, colposcopy, high-risk HPV-DNA test and cervical bioscopy. RESUTS In 418 cases of vulva condyloma acuminatum, verified by pathologic analysis, high-risk HPV(+) infections were detected in 68.7%(287/418) of the cases. Among those patients, 20.6% (59/287) had concurrent subclinical cervical intraepithelial neoplastic (CIN) lesions. Among the high-risk HPV(-) 31.3 % (131), patients 7.6% (10/131) had concurrent subclinical CIN lesions. Pathological examination results: cervicitis, 167 (40.0%); cervical HPV infection, 182 (43.5%); CIN-I, 51 (12.2%); CIN-II, 16(3.83%); CIN-III, 2 (0.5%); cervical cancer, none. Another patient had vulva condyloma acuminatum with valva intraepithelial neoplasia (VIN) II^III. CONCLUSION It was concluded that simultaneous cervical HPV infection of many types was rather common in patients with vulva condyloma acuminatum. Vulva condyloma acuminatum is the chief clinical symptom which hints at a high possibility of infection with high-risk HPV. The patients are at high-risk for CIN and cervical cancer. We must pay more attention to the cervix in cases with vulva condyloma acuminatum.展开更多
Objective:Human papillomavirus(HPV)causes condyloma acuminata,which is one of the most widespread sexually transmitted diseases.This study was aimed to evaluate the prevalence of HPV infection and HPV genotype distrib...Objective:Human papillomavirus(HPV)causes condyloma acuminata,which is one of the most widespread sexually transmitted diseases.This study was aimed to evaluate the prevalence of HPV infection and HPV genotype distribution in patients with condyloma acuminatum in Urumqi,China.Methods:A total of 835 cervical and genital samples were collected from outpatients treated for condyloma acuminatum between December 2016 and December 2018.HPV genotyping was performed with the HPV Genotyping Real-time PCR Kit for 23 different HPV types.Statistcal analysis was conducted using the chi-square test.Results:The total prevalence of HPV infection was 80.72%(674/835)in patients with condyloma acuminatum in Urumqi.The HPV genotypes were HPV-6(372/835,44.55%),HPV-11(180/835,21.56%),HPV-16(91/835,10.90%),HPV-43(65/835,7.78%),HPV-42(56/835,6.71%),and HPV-58(53/835,6.35%).Among HPV-infected individuals,49.11%(331/674)were infected by a single HPV subtype,while 50.89%(343/674)were infected by multiple HPV subtypes.The prevalence of HPV infection was higher in females(82.14%,276/336)than in males(79.76%,398/499)(χ^(2)=0.73,P=0.39),and higher in other ethnicities(86.36%,133/154)than in people of Han ethnicity(79.76%,541/681)(χ^(2)=3.866,P=0.049).The peak prevalence of HPV infection was observed in the age groups of 20-29 years and 30-39 years.Conclusion:HPV infection was detected in most patients with condyloma acuminatum in Urumqi with prevalence of 80.72%,and the most common HPV types were HPV-6,-11,-16,-43,-42,and-58.The detection of HPV genotypes is very important to monitor HPV infection so that effective treatment is administered to patients with condyloma acuminatum.展开更多
文摘Summary: The telomerse activity in condyloma acuminatum (CA) tissue with human papillomavirus (HPV) types of 6/11 and 16/18 was detected to investigate the function of telomerase in the occurrence, development and carcinogenesis of genital CA. Forty-two biopsies from patients with gennital CA and 30 control tissue samples were tested for telomerase activity, HPV presence and types. The telomerase activity was determined by modified telomerase repeat amplification protocol (TRAP) assay and HPV typing by polymerase chain reaction (PCR) with typing-specific primers. Results showed that HPV-DNA was negative and the expression rate of telomerase was 16.7 % in all normal skin samples. All CA samples were positive for HPV (6/11 type was found in 32 cases, 16/18 in 3 and mixed type in 7). Telomerase activity was detectable in all CA patients. The telomerase activity in CA of 16/18 type was apparently higher than in CA of 6/11 type. It was concluded that the hyperplasia in CA might be increased as a result of HPV infection, suggesting that the activation of telomerase by HPV, especially by 16/18 type may play a role in the etiology and carcinogenesis of genital CA.
文摘OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus (HPV) infections and cervical epithelium lesions. METHODS From May 2002 to April 2004 patients with vulva condyloma acuminatum were examined employing vulva biospy, colposcopy, high-risk HPV-DNA test and cervical bioscopy. RESUTS In 418 cases of vulva condyloma acuminatum, verified by pathologic analysis, high-risk HPV(+) infections were detected in 68.7%(287/418) of the cases. Among those patients, 20.6% (59/287) had concurrent subclinical cervical intraepithelial neoplastic (CIN) lesions. Among the high-risk HPV(-) 31.3 % (131), patients 7.6% (10/131) had concurrent subclinical CIN lesions. Pathological examination results: cervicitis, 167 (40.0%); cervical HPV infection, 182 (43.5%); CIN-I, 51 (12.2%); CIN-II, 16(3.83%); CIN-III, 2 (0.5%); cervical cancer, none. Another patient had vulva condyloma acuminatum with valva intraepithelial neoplasia (VIN) II^III. CONCLUSION It was concluded that simultaneous cervical HPV infection of many types was rather common in patients with vulva condyloma acuminatum. Vulva condyloma acuminatum is the chief clinical symptom which hints at a high possibility of infection with high-risk HPV. The patients are at high-risk for CIN and cervical cancer. We must pay more attention to the cervix in cases with vulva condyloma acuminatum.
基金supported by the Technology Introduction and Innovation Projects of Peoples Hospital of Xinjiang Uygur Autonomous Region(No.20180304)。
文摘Objective:Human papillomavirus(HPV)causes condyloma acuminata,which is one of the most widespread sexually transmitted diseases.This study was aimed to evaluate the prevalence of HPV infection and HPV genotype distribution in patients with condyloma acuminatum in Urumqi,China.Methods:A total of 835 cervical and genital samples were collected from outpatients treated for condyloma acuminatum between December 2016 and December 2018.HPV genotyping was performed with the HPV Genotyping Real-time PCR Kit for 23 different HPV types.Statistcal analysis was conducted using the chi-square test.Results:The total prevalence of HPV infection was 80.72%(674/835)in patients with condyloma acuminatum in Urumqi.The HPV genotypes were HPV-6(372/835,44.55%),HPV-11(180/835,21.56%),HPV-16(91/835,10.90%),HPV-43(65/835,7.78%),HPV-42(56/835,6.71%),and HPV-58(53/835,6.35%).Among HPV-infected individuals,49.11%(331/674)were infected by a single HPV subtype,while 50.89%(343/674)were infected by multiple HPV subtypes.The prevalence of HPV infection was higher in females(82.14%,276/336)than in males(79.76%,398/499)(χ^(2)=0.73,P=0.39),and higher in other ethnicities(86.36%,133/154)than in people of Han ethnicity(79.76%,541/681)(χ^(2)=3.866,P=0.049).The peak prevalence of HPV infection was observed in the age groups of 20-29 years and 30-39 years.Conclusion:HPV infection was detected in most patients with condyloma acuminatum in Urumqi with prevalence of 80.72%,and the most common HPV types were HPV-6,-11,-16,-43,-42,and-58.The detection of HPV genotypes is very important to monitor HPV infection so that effective treatment is administered to patients with condyloma acuminatum.
