期刊文献+
共找到50篇文章
< 1 2 3 >
每页显示 20 50 100
Calculation of Mass Concrete Temperature and Creep Stress under the Influence of Local Air Heat Transfer
1
作者 Heng Zhang Chao Su +2 位作者 Xiaohu Chen Zhizhong Song Weijie Zhan 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第9期2977-3000,共24页
Temperature-induced cracking during the construction of mass concrete is a significant concern.Numerical simulations of concrete temperature have primarily assumed that the concrete is placed in an open environment.Th... Temperature-induced cracking during the construction of mass concrete is a significant concern.Numerical simulations of concrete temperature have primarily assumed that the concrete is placed in an open environment.The problem of heat transfer between the air and concrete has been simplified to the concrete’s heat dissipation boundary.However,in the case of tubular concrete structures,where air inlet and outlet are relatively limited,the internal air temperature does not dissipate promptly to the external environment as it rises.To accurately simulate the temperature and creep stress in tubular concrete structures with enclosed air spaces during construction,we establish an air–concrete coupled heat transfer model according to the principles of conjugate heat transfer,and the accuracy of the model is verified through experiments.Furthermore,we conduct a case study to analyze the impact of airflow within the ship lock corridor on concrete temperature and creep stress.The results demonstrate that enhancing airflow within the corridor can significantly reduce the maximum concrete temperature.Compared with cases in which airflow within the corridor is neglected,the maximum concrete temperature and maximum tensile stress can be reduced by 12.5℃ and 0.7 MPa,respectively,under a wind speed of 4 m/s.The results of the traditional calculation method are relatively close to those obtained at a wind speed of 1 m/s.However,the temperature reduction process in the traditional method is faster,and the method yields greater tensile stress values for the corridor location. 展开更多
关键词 Conjugate heat transfer temperature field mass concrete creep stress
下载PDF
Herbicide promotes the conjugative transfer of multi-resistance genes by facilitating cellular contact and plasmid transfer 被引量:3
2
作者 Xi Li Chang Wen +6 位作者 Chen Liu Shiyun Lu Zhongbing Xu Qiue Yang Zhi Chen Hanpeng Liao Shungui Zhou 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2022年第5期363-373,共11页
The global dissemination of antibiotic resistance genes(ARGs),especially via plasmid-mediated horizontal transfer,is becoming a pervasive health threat.While our previous study found that herbicides can accelerate the... The global dissemination of antibiotic resistance genes(ARGs),especially via plasmid-mediated horizontal transfer,is becoming a pervasive health threat.While our previous study found that herbicides can accelerate the horizontal gene transfer(HGT)of ARGs in soil bacteria,the underlying mechanisms by which herbicides promote the HGT of ARGs across and within bacterial genera are still unclear.Here,the underlying mechanism associ-ated with herbicide-promoted HGT was analyzed by detecting intracellular reactive oxygen species(ROS)production,extracellular polymeric substance composition,cell membrane integrity and proton motive force combined with genome-wide RNA sequencing.Exposure to herbicides induced a series of the above bacterial responses to promote HGT except for the ROS response,including compact cell-to-cell contact by enhancing pilus-encoded gene expression and decreasing cell surface charge,increasing cell membrane permeability,and enhancing the proton motive force,providing additional power for DNA uptake.This study provides a mechanistic understanding of the risk of bacterial resistance spread promoted by herbicides,which elucidates a new perspective on nonantibiotic agrochemical acceleration of the HGT of ARGs. 展开更多
关键词 HERBICIDES Horizontal gene transfer Conjugation transfer Antibiotic resistance genes Cell membrane permeability
原文传递
Lattice Boltzmann simulation of the effects of cavity structures and heater thermal conductivity on nucleate boiling heat transfer
3
作者 Fanming CAI Zhaomiao LIU +2 位作者 Nan ZHENG Yanlin REN Yan PANG 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2023年第6期981-996,共16页
The boiling heat transfer technology with cavity surfaces can provide higher heat flux under lower wall superheat,which is of great significance for the cooling of electronic chips and microelectromechanical devices.I... The boiling heat transfer technology with cavity surfaces can provide higher heat flux under lower wall superheat,which is of great significance for the cooling of electronic chips and microelectromechanical devices.In this paper,the boiling characteristics of the cavity surfaces are investigated based on the lattice Boltzmann(LB)method,focusing on the effects of cavity shapes,sizes,and heater thermal conductivity on the heat transfer performance.The results show that the triangular cavity has the best boiling performance since it has less residual vapor and higher bubble departure frequency than those of the trapezoidal and rectangular cavities.As the cavity size increases,the enhancement of heat transfer by the cavity mouth is suppressed by the heat accumulation effect at the heater bottom.The liquid rewetting process during bubble departure is the reason for the fluctuation of the space-averaged heat flux,and the heater thermal conductivity determines the fluctuation amplitude.The evaporation of liquid in the cavity with high thermal conductivity walls is more intense,resulting in shorter waiting time and higher bubble departure frequency. 展开更多
关键词 lattice Boltzmann(LB)method BOILING CAVITY conjugate heat transfer
下载PDF
Conjugate Heat Transfer Analysis of an Ultrasonic Molten Metal Treatment System 被引量:1
4
作者 ZHU Youli BIAN Feilong +1 位作者 WANG Yanli ZHAO Qian 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2014年第5期986-991,共6页
In piezoceramic ultrasonic devices,the piezoceramic stacks may fail permanently or function improperly if their working temperatures overstep the Curie temperature of the piezoceramic material.While the end of the hor... In piezoceramic ultrasonic devices,the piezoceramic stacks may fail permanently or function improperly if their working temperatures overstep the Curie temperature of the piezoceramic material.While the end of the horn usually serves near the melting point of the molten metal and is enclosed in an airtight chamber,so that it is difficult to experimentally measure the temperature of the transducer and its variation with time,which bring heavy difficulty to the design of the ultrasonic molten metal treatment system.To find a way out,conjugate heat transfer analysis of an ultrasonic molten metal treatment system is performed with coupled fluid and heat transfer finite element method.In modeling of the system,the RNG model and the SIMPLE algorithm are adopted for turbulence and nonlinear coupling between the momentum equation and the energy equation.Forced air cooling as well as natural air cooling is analyzed to compare the difference of temperature evolution.Numerical results show that,after about 350 s of working time,temperatures in the surface of the ceramic stacks in forced air cooling drop about 7 K compared with that in natural cooling.At 240 s,The molten metal surface emits heat radiation with a maximum rate of about 19 036 W/m2,while the heat insulation disc absorbs heat radiation at a maximum rate of about 7922 W/m2,which indicates the effectiveness of heat insulation of the asbestos pad.Transient heat transfer film coefficient and its distribution,which are difficult to be measured experimentally are also obtained through numerical simulation.At 240 s,the heat transfer film coefficient in the surface of the transducer ranges from–17.86 to 20.17 W/(m2?K).Compared with the trial and error method based on the test,the proposed research provides a more effective way in the design and analysis of the temperature control of the molten metal treatment system. 展开更多
关键词 conjugate heat transfer finite element analysis ultrasonic molten metal treatment computational fluid dynamics
下载PDF
Streamline upwind finite element method for conjugate heat transfer problems 被引量:3
5
作者 Niphon Wansophark Atipong Malatip Pramote Dechaumphai 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2005年第5期436-443,共8页
This paper presents a combined finite element method for solving conjugate heat transfer problems where heat conduction in a solid is coupled with heat convection in viscous fluid flow. The streamline upwind finite el... This paper presents a combined finite element method for solving conjugate heat transfer problems where heat conduction in a solid is coupled with heat convection in viscous fluid flow. The streamline upwind finite element method is used for the analysis of thermal viscous flow in the fluid region, whereas the analysis of heat conduction in solid region is performed by the Galerkin method. The method uses the three-node triangular element with equal-order interpolation functions for all the variables of the velocity components, the pressure and the temperature. The main advantage of the proposed method is to consistently couple heat transfer along the fluid-solid interface. Three test cases, i.e. conjugate Couette flow problem in parallel plate channel, counter-flow in heat exchanger, and conjugate natural convection in a square cavity with a conducting wall, are selected to evaluate the efficiency of the present method. 展开更多
关键词 Streamline upwind.Conjugate heat transfer.Finite element method
下载PDF
Numerical Simulation and Control of Two-Phase Flow with Evaporation in a Vertical Tube Submitted to a Conjugate Heat Transfer 被引量:2
6
作者 Ghazali Mebarki Samir Rahal 《Journal of Energy and Power Engineering》 2013年第7期1282-1292,共11页
A better understanding of two-phase flows with evaporation allows leading to an optimal design of evaporators. For that purpose, numerical simulations are very useful. In this paper, a numerical study has been carried... A better understanding of two-phase flows with evaporation allows leading to an optimal design of evaporators. For that purpose, numerical simulations are very useful. In this paper, a numerical study has been carried out in order to model and simulate the combination of a two-phase flow with evaporation in a vertical tube. The VOF (volume-of-fluid) multiphase flow method and a phase-change model for the mass transfer have been used. For an accurate modeling, the effect of axial conduction has been also taken into account using a conjugate heat transfer model. Since thermal oscillations are undesirable as they can lead to the failure of the tube, flow instabilities have also been analyzed, using FFT (fast Fourier transforms), in order to comprehend their behavior and influence. A control study of the flow instabilities in the tube is also presented. For that purpose tube inlet temperature has been varied using a gain control parameter. 展开更多
关键词 Two-phase flow EVAPORATION VOF method conjugate heat transfer flow instabilities control of instabilities.
下载PDF
Numerical simulation on conjugate heat transfer of turbine cascade
7
作者 周驰 颜培刚 +1 位作者 姜澎 冯国泰 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2009年第6期741-745,共5页
Numerical simulation on conjugate heat transfer of an internal cooled turbine vane was carried out. Numerical techniques employed included the third-order accuracy TVD scheme, multi-block structured grids and the tech... Numerical simulation on conjugate heat transfer of an internal cooled turbine vane was carried out. Numerical techniques employed included the third-order accuracy TVD scheme, multi-block structured grids and the technique of arbitrary curved mesh. Comparison between results of commercial CFD codes with several turbulence models and those of this code shows that it is incorrect of commercial CFD codes to predict the thermal boundary layer with traditional turbulence models, and that turbulence models considering transition lead to more accurate heat transfer in thermal boundary layer with some reliability and deficiency yet. The results of this code are close to those of CFX with transition model. 展开更多
关键词 TURBINE conjugate heat transfer heat conduction numerical validation
下载PDF
Investigation of convection cooling guide vane with conjugate heat transfer method
8
作者 陈凯 黄洪雁 +1 位作者 韩万金 冯国泰 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2010年第4期456-460,共5页
This paper studied a certain blade with ten radial cooling holes which employed conjugate heat transfer method. The cooling air entered the cooling channel from the bottom of the blade and went out from the top, it wa... This paper studied a certain blade with ten radial cooling holes which employed conjugate heat transfer method. The cooling air entered the cooling channel from the bottom of the blade and went out from the top, it was not ejected into the main flow. This paper used different numerical conditions including different turbulence models,turbulence intensities,thermal conduction coefficients and the influence on fluid property via temperature variation. The temperature distribution and pressure distribution of the blade were compared with experimental data. The results show that the numerical results using different turbulence models are almost identical to experimental data even little deviation occurs at shock wave location. The trends of temperature distribution under different numerical conditions are coincident to experimental data,especially Reynolds stress turbulence model. It can be concluded that anisotropic turbulence models can simulate the transition from laminar to turbulence,and the influence of turbulence intensity on laminar region and transition region is more than that on developed turbulent region. 展开更多
关键词 conjugate heat transfer air cooling turbine convection cooling numerical simulation
下载PDF
A new algorithm of global tightly-coupled transient heat transfer based on quasi-steady flow to the conjugate heat transfer problem
9
作者 Fanchao Meng Sujun Dong +1 位作者 Jun Wang Dechun Guo 《Theoretical & Applied Mechanics Letters》 CAS CSCD 2016年第5期233-235,共3页
Concerning the specific demand on solving the long-term conjugate heat transfer (CHT) problem, a new algorithm of the global tightly-coupled transient heat transfer based on the quasi-steady flow field is further pu... Concerning the specific demand on solving the long-term conjugate heat transfer (CHT) problem, a new algorithm of the global tightly-coupled transient heat transfer based on the quasi-steady flow field is further put forward. Compared to the traditional loosely-coupled algorithm, the computational efficiency is further improved with the greatly reduced update frequency of the flow field, and moreover the update step of the flow field can be reasonably determined by using the engineering empirical formula of the Nusselt number based on the changes of the inlet and outlet boundary conditions. Taking a duct heated by inner forced air flow heating process as an example, the comparing results to the tightly-coupled transient calculation by Fluent software shows that the new algorithm can significantly improve the computational efficiency with a reasonable accuracy on the transient temperature distribution, such as the computing time is reduced to 22,8% and 40% while the duct wall temperature deviation are 7% and 5% respectively using two flow update time step of 100 s and 50 s on the variable inlet-flow rate conditions. 展开更多
关键词 Conjugate heat transfer Loosely-coupledQuasi-steady Computational fluid dynamics
下载PDF
Numerical Study of Conjugate Heat Transfer for Cooling the Circuit Board
10
作者 Abdullah Alrashidi 《Journal of Electronics Cooling and Thermal Control》 2016年第3期120-126,共8页
In this paper, a 3D model of a flat circuit board with a heat generating electronic chip mounted on it has been studied numerically. The conjugate heat transfer including the conduction in the chip and convection with... In this paper, a 3D model of a flat circuit board with a heat generating electronic chip mounted on it has been studied numerically. The conjugate heat transfer including the conduction in the chip and convection with the surrounding fluid has been investigated numerically. Computational fluid dynamics using the finite volume method has been used for modeling the conjugate heat transfer through the chip and the circuit board. Conjugate heat transfer has broad applications in engineering and industrial applications in design of cooling off electronic components. Effects of various inlet velocities have been studied on the heat transfer variation and temperature of the circuit board. Numerical results show that the temperature of the chip reduces as the velocity of the inlet fluid flow increases. 展开更多
关键词 Conjugate Heat transfer Circuit Board Numerical Simulation Mass Flow Rate 3D Model
下载PDF
Heat Transfer and Flow Structure of a Turbine Blade's Air-cooled Leading Edge Considering Different Hole Shapes and Additional Flow Angles
11
作者 QIN Runxuan ZHOU Xun +1 位作者 WANG Songtao CAI Le 《Journal of Thermal Science》 SCIE EI CAS CSCD 2024年第4期1421-1442,共22页
A numerical study is conducted to elucidate the impact of hole shapes and additional flow angles on the flow structure of the coolant and temperature field in the leading edge area of the gas turbine rotor.Four typica... A numerical study is conducted to elucidate the impact of hole shapes and additional flow angles on the flow structure of the coolant and temperature field in the leading edge area of the gas turbine rotor.Four typical hole shapes are considered for the GE-E3 blade.The impact of the additional flow angle(E)within each hole shape on the temperature field is investigated.The results indicate that for the leading edge area and suction surface,the fan-shaped hole case performs best in decreasing temperatures,with a decrease of about 43 K.This is mainly due to the fact that the fan-shaped hole has the maximum expansion in hole spanwise direction.For the pressure surface,the console hole case performs best in decreasing temperatures,with a maximum reduction of about 47.2 K.The influence of E on the surface temperature at leading edge area varied between the different hole shapes.For the cylinder hole and console hole,the E=-20°case has the lowest area-averaged temperature.Because both the fan-shaped hole and the 7-7-7 shaped hole are expansion holes,the pattern of variation of the leading edge area temperature with increasing E is similar for the fan-shaped hole case and 7-7-7 shaped hole case.The E=20°case shows the lowest spanwise-averaged temperature near the hole outlet,and the E=-20°case shows the lowest spanwise-averaged temperature further downstream. 展开更多
关键词 film cooling GE-E3 turbine rotor blade conjugate heat transfer leading edge
原文传递
Stimulants and donors promote megaplasmid pND6-2 horizontal gene transfer in activated sludge
12
作者 Shan Wang Shanshan Li +3 位作者 Dan Du Olusegun KAbass Muhammad Salman Nasir Wei Yan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第4期742-753,共12页
The activated sludge process is characterized by high microbial density and diversity,both of which facilitate antibiotic resistance gene transfer.Many studies have suggested that antibiotic and non-antibiotic drugs a... The activated sludge process is characterized by high microbial density and diversity,both of which facilitate antibiotic resistance gene transfer.Many studies have suggested that antibiotic and non-antibiotic drugs at sub-inhibitory concentrations are major inducers of conjugative gene transfer.The self-transmissible plasmid pND6-2 is one of the endogenous plasmids harbored in Pseudomonas putida ND6,which can trigger the transfer of another co-occurring naphthalene-degrading plasmid pND6-1.Therefore,to illustrate the potential influence of stimulants on conjugative transfer of pND6-2,we evaluated the effects of four antibiotics (ampicillin,gentamycin,kanamycin,and tetracycline) and naphthalene,on the conjugal transfer efficiency of pND6-2 by filter-mating experiment.Our findings demonstrated that all stimulants within an optimal dose promoted conjugative transfer of pND6-2from Pseudomonas putida GKND6 to P.putida KT2440,with tetracycline being the most effective (100μg/L and 10μg/L),as it enhanced pND6-2-mediated intra-genera transfer by approximately one hundred-fold.Subsequently,seven AS reactors were constructed with the addition of donors and different stimulants to further elucidate the conjugative behavior of pND6-2 in natural environment.The stimulants positively affected the conjugal process of pND6-2,while donors reshaped the host abundance in the sludge.This was likely because stimulant addition enhanced the expression levels of conjugation transfer-related genes.Furthermore,Blastocatella and Chitinimonas were identified as the potential receptors of plasmid pND6-2,which was not affected by donor types.These findings demonstrate the positive role of sub-inhibitory stimulant treatment on pND6-2 conjugal transfer and the function of donors in re-shaping the host spectrum of pND6-2. 展开更多
关键词 ANTIBIOTICS NAPHTHALENE DONOR conjugative transfer Host range
原文传递
Study on seepage and deformation characteristics of coal microstructure by 3D reconstruction of CT images at high temperatures 被引量:19
13
作者 Gang Wang Xiangjie Qin +1 位作者 Dongyang Han Zhiyuan Liu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2021年第2期175-185,共11页
To study the seepage and deformation characteristics of coal at high temperatures,coal samples from six different regions were selected and subjected to computed tomography(CT)scanning studies.In conjunction with ANSY... To study the seepage and deformation characteristics of coal at high temperatures,coal samples from six different regions were selected and subjected to computed tomography(CT)scanning studies.In conjunction with ANSYS software,3 D reconstruction of CT images was used for the establishment of fluidsolid conjugate heat transfer model and coal thermal deformation model based on the microstructures of coal.In addition,the structure of coal was studied in 2 D and 3 D perspectives,followed by the analysis of seepage and deformation characteristics of coal at high temperatures.The results of this study indicated that porosity positively correlated with the fractal dimension,and the connectivity and seepage performances were roughly identical from 2 D and 3 D perspectives.As the porosity increased,the fractal dimension of coal samples became larger and the pore-fracture structures became more complex.As a result,the permeability of coal samples decreased.In the meantime,fluid was fully heated,generating high-temperature water at outlet.However,when the porosity was low,the outlet temperature was very high.The average deformation of coal skeleton with different pore-fracture structures at high temperatures showed a trend of initial increase and subsequent decrease with the increase of porosity and fractal dimension.The maximum deformation of coal skeleton positively correlated with connectivity but negatively correlated with the fractal dimension. 展开更多
关键词 COAL Pore-fracture structures Fluid-solid conjugate heat transfer Thermal deformation
下载PDF
Radiator Optimization Design for Planar Motors Based on Parametric Components 被引量:1
14
作者 Ming Zhang Qiaomu Xu +2 位作者 Rong Cheng Leijie Wang Xin Li 《Journal of Beijing Institute of Technology》 EI CAS 2020年第2期222-231,共10页
Focusing on the design problem of high-performance radiators for planar motors in the wafer stage of the lithography machine,a thermal-fluid coupling optimization scheme based on parametric solid components was propos... Focusing on the design problem of high-performance radiators for planar motors in the wafer stage of the lithography machine,a thermal-fluid coupling optimization scheme based on parametric solid components was proposed.The mapping method between component parameters and pseudo-density values was established.An analytical solution for the sensitivity of pseudo-density to component parameters was given.The conjugate heat transfer function with the shallow channel approximation term was solved through the pseudo-density information.In the optimization example,circular components were selected,and the position and the size of solid components were chosen as design variables.In order to eliminate calculation errors caused by pseudo-density,an optimized pseudo-density field was converted into the result based on parametric components.Compared to the reference motor radiator,the average surface temperature rise of the optimized water-cooling motor radiator is reduced by 22.4%,which verifies the feasibility and effectiveness of the proposed method. 展开更多
关键词 motor cooling conjugate heat transfer parametric components shallow channel approximation
下载PDF
Effect of sulfamethazine on the horizontal transfer of plasmid-mediated antibiotic resistance genes and its mechanism of action 被引量:1
15
作者 Xiaojing Yan Wenwen Liu +5 位作者 Shengfang Wen Lanjun Wang Lusheng Zhu Jun Wang Young Mo Kim Jinhua Wang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第5期399-409,共11页
As a new type of environmental pollutant,antibiotic resistance genes(ARGs)pose a huge challenge to global health.Horizontal gene transfer(HGT)represents an important route for the spread of ARGs.The widespread use of ... As a new type of environmental pollutant,antibiotic resistance genes(ARGs)pose a huge challenge to global health.Horizontal gene transfer(HGT)represents an important route for the spread of ARGs.The widespread use of sulfamethazine(SM2)as a broad-spectrum bacteriostatic agent leads to high residual levels in the environment,thereby increasing the spread of ARGs.Therefore,we chose to study the effect of SM2 on the HGT of ARGs mediated by plasmid RP4 from Escherichia coli(E.coli)HB101 to E.coli NK5449 as well as its mechanism of action.The results showed that compared with the control group,SM2 at concentrations of 10 mg/L and 200 mg/L promoted the HGT of ARGs,but transfer frequency decreased at concentrations of 100 mg/L and 500 mg/L.The transfer frequency at 200 mg/L was 3.04×10^(−5),which was 1.34-fold of the control group.The mechanism of SM2 improving conjugation transfer is via enhancement of the mRNA expression of conjugation genes(trbBP,trfAP)and oxidative stress genes,inhibition of the mRNA expression of vertical transfer genes,up regulation of the outer membrane protein genes(ompC,ompA),promotion of the formation of cell pores,and improvement of the permeability of cell membrane to promote the conjugation transfer of plasmid RP4.The results of this study provide theoretical support for studying the spread of ARGs in the environment. 展开更多
关键词 SULFAMETHAZINE PLASMID Antibiotic resistance gene Conjugation transfer
原文传递
Expression of organophosphorus-degradation gene(opd) in aggregating and non-aggregating filamentous nitrogen-fixing cyanobacteria
16
作者 李琼 唐蜻 +1 位作者 徐旭东 高宏 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第6期1248-1253,共6页
Genetic engineering in filamentous N2-fixing cyanobacteria usually involves Anabaena sp. PCC 7120 and several other non-aggregating species. Mass culture and harvest of such species are more energy consuming relative ... Genetic engineering in filamentous N2-fixing cyanobacteria usually involves Anabaena sp. PCC 7120 and several other non-aggregating species. Mass culture and harvest of such species are more energy consuming relative to aggregating species. To establish a gene transfer system for aggregating species, we tested many species of Anabaena and Nostoc, and identified Nostoc muscorum FACHB244 as a species that can be genetically manipulated using the conjugative gene transfer system. To promote biodegradation of organophosphorus pollutants in aquatic environments, we introduced a plasmid containing the organophosphorus-degradation gene (opd) into Anabaena sp. PCC 7120 and Nostoc muscorum FACHB244 by conjugation. The opd gene was driven by a strong promoter, Pp,bA. From both species, we obtained transgenic strains having organophosphorus-degradation activities. At 25~C, the whole-cell activities of the transgenic Anabaena and Nostoc strains were 0.163~0.001 and 0.289~0.042 unit/gg Chl a, respectively. However, most colonies resulting from the gene transfer showed no activity. PCR and DNA sequencing revealed deletions or rearrangements in the plasmid in some of the colonies. Expression of the green fluorescent protein gene from the same promoter in Anabaena sp. PCC 7120 showed similar results. These results suggest that there is the potential to promote the degradation of organophosphorus pollutants with transgenic cyanobacteria and that selection of high-expression transgenic colonies is important for genetic engineering of Anabaena and Nostoc species. For the first time, we established a gene transfer and expression system in an aggregating filamentous N2-fixing cyanobacterium. The genetic manipulation system of Nostoc muscorum FACHB244 could be utilized in the elimination of pollutants and large-scale production of valuable proteins or metabolites. 展开更多
关键词 CYANOBACTERIA aggregating species conjugative gene transfer organophosphorus-degradation
下载PDF
Numerical simulation of HP rotor cooling configuration using parameterized method
17
作者 周鸿儒 顾忠华 +1 位作者 韩万金 刘占生 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2011年第2期134-138,共5页
This paper implemented cooling configuration design on certain gas turbine HP rotor using parameterized method.It is convenient for complicated gas turbine blade modeling using parameters and also benefit for the geom... This paper implemented cooling configuration design on certain gas turbine HP rotor using parameterized method.It is convenient for complicated gas turbine blade modeling using parameters and also benefit for the geometry modify in later period.Parameterized modeling is the foundation of air cooling turbine blade design method engineering application.Mesh quality can be awarded when generated complicated cooling configuration blade grids,and also the increase of calculation error can arise by many mesh blocks.Film cooling and serpentine passage can effectively enhance the cooling effectiveness and protect blade. 展开更多
关键词 conjugate heat transfer air cooling turbine parameterized method ROTOR
下载PDF
Numerical Investigation of Flow and Heat Transfer in Vane Impingement/Effusion Cooling with Various Rib/Dimple Structure
18
作者 WANG Mingrui ZHU Huiren +3 位作者 LIU Cunliang WANG Rui WU Zhuang YAO Chunyi 《Journal of Thermal Science》 SCIE EI CAS CSCD 2023年第4期1357-1377,共21页
By investigating heat transfer and flow structures of dimples,orthogonal ribs,and V-shaped ribs in the impingement/effusion cooling,the article is dedicated to selecting a best-performing internal cooling structure fo... By investigating heat transfer and flow structures of dimples,orthogonal ribs,and V-shaped ribs in the impingement/effusion cooling,the article is dedicated to selecting a best-performing internal cooling structure for a turbine vane.The overall cooling effectiveness and coolant consumption are adopted to evaluate the cooling performance.To analyze the influence of structural modification,the flow field is investigated on chordwise/spanwise sections and the target surface.The blockage effect on crossflow can protect jet flow,resulting in higher heat transfer performance of the target surface.Ribs own a stronger blockage effect than dimples.Compared with the blockage effect,the influence of the rib shape is negligible.By installing dimples between ribs,heat transfer is augmented further.The introduction of ribs/dimples leads to higher discharge coefficients of jet nozzles but lower discharge coefficients of film holes.Thus,the film cooling deteriorates.Meanwhile,the installation of the ribs and dimples decreases total coolant consumption.The effect of ribs/dimples on heat transfer and effusion condition of internal and external cooling is analyzed.The best-performing cooling structure is the target surface with dimples and orthogonal ribs,which decreases the wall temperature and coolant consumption by 14.57-28.03 K and 1.19%-1.81%respectively.This article concludes the flow mechanism for dimples and influence factors on the cooling performance,which may serve as guidance for the turbine vane design. 展开更多
关键词 conjugate heat transfer impingement cooling DIMPLE V-shape rib discharge coefficient
原文传递
Numerical Simulation of Two-Phase Closed Thermosyphon
19
作者 G.V. Kuznetsov M.A. Al-Ani M.A. Sheremet 《Journal of Energy and Power Engineering》 2011年第3期227-232,共6页
Heat transfer and hydrodynamics of two phase closed thermosyphon (TPCT) is studied using finite difference method of Stream function and vorticity. The mathematical model is formed for both vapor phase and liquid fi... Heat transfer and hydrodynamics of two phase closed thermosyphon (TPCT) is studied using finite difference method of Stream function and vorticity. The mathematical model is formed for both vapor phase and liquid film in a non-dimensional form. The momentum and energy equations as parabolic equations have been solved by means of the locally one-dimensional scheme of A.A. Samarskii. The Poisson equation for the stream function has been approximated on the basis of the discrete scheme "cross". The obtained system of algebraic equations has been solved by the successive over relaxation method. The effect of the Rayleigh number on the velocity and temperature fields in the domain of interest and on the average Nusselt number at the solid-fluid interfaces is investigated. It was found that increase in the Rayleigh number leads to the increment of both the average Nusselt number and the stream function in the vapor zone. 展开更多
关键词 Mathematical simulation two-phase thermosyphon conjugate heat transfer hydrodynamics.
下载PDF
Unified Solution of Conjugate Fluid and Solid Heat Transfer-Part I.Solid Heat Conduction
20
作者 Shujie Li Lili Ju 《Advances in Applied Mathematics and Mechanics》 SCIE 2023年第3期814-830,共17页
A unified solution framework is proposed for efficiently solving conjugate fluid and solid heat transfer problems.The unified solution is solely governed by the compressible Navier-Stokes(N-S)equations in both fluid a... A unified solution framework is proposed for efficiently solving conjugate fluid and solid heat transfer problems.The unified solution is solely governed by the compressible Navier-Stokes(N-S)equations in both fluid and solid domains.Such method not only provides the computational capability for solid heat transfer simulations with existing successful N-S flow solvers,but also can relax time-stepping restrictions often imposed by the interface conditions for conjugate fluid and solid heat transfer.This paper serves as Part I of the proposed unified solution framework and addresses the handling of solid heat conduction with the nondimensional N-S equations.Specially,a parallel,adaptive high-order discontinuous Galerkin unified solver has been developed and applied to solve solid heat transfer problems under various boundary conditions. 展开更多
关键词 Conjugate heat transfer solid heat conduction compressible Navier-Stokes exponential time integration discontinuous Galerkin
原文传递
上一页 1 2 3 下一页 到第
使用帮助 返回顶部