To explore the structural characteristic of ribosomal protein S29 (rpS29) gene of Ailuropoda melanoleuca (giant panda), primers were designed based on the known nucleotide sequence of rpS29 genes to clone the cDNA and...To explore the structural characteristic of ribosomal protein S29 (rpS29) gene of Ailuropoda melanoleuca (giant panda), primers were designed based on the known nucleotide sequence of rpS29 genes to clone the cDNA and genomic sequences of this gene from giant panda by RT-PCR and PCR strategy respectively, and then the cloned cDNA and genomic sequences were sequenced and analyzed preliminarily. The results indicated that the cDNA fragment of the rpS29 from the giant panda is 205bp in size in length, containing an open reading frame (ORF) of 171bp, encoding 56 amino acids. The length of the genomic sequence is 1 598 bp, with three exons and two introns. The coding sequence shows a high degree of homology to those of Homo sapiens, Bos taurus, Rattus norvegicus and Mus musculus with 75%, 94.74%, 89.47% and 88.89% respectively, While all the homologies for amino acid sequences are high up to 100%. Primary structure analysis revealed that the molecular weight of the putative rpS29 protein is 6.68 KD, with a theoretical pI of 10.63. Based on topology prediction, there are three distinct types of functional sites in the rpS29 protein of giant panda.展开更多
Primers were designed according to the known sequences of Sterol Regulatory Element Binding Proteins-1c (SREBP-1c) genes of human, rat and pig. RT-PCR was then used to amplify porcine SREBP-1c gene with total RNA of s...Primers were designed according to the known sequences of Sterol Regulatory Element Binding Proteins-1c (SREBP-1c) genes of human, rat and pig. RT-PCR was then used to amplify porcine SREBP-1c gene with total RNA of spleen tissue. A 760 bp segment of cDNA was cloned and sequenced. Homogeneous comparison showed that the sequence of porcine SREBP-1c had 99.9% and 99.1% homogeneity with the two known partial mRNA sequences of porcine SREBP-1c gene. The complete cDNA was obtained mainly based on the known partial sequences, which has 3 830 bp, encoding 1 151 amino acids with a calculated molecular weight of 121 479 Da. It is the first time that we get complete encode sequence of porcine SREBP-1c gene. The complete cDNA sequence has high homogeneity with SREBP-1c gene of other species. A characteristic structure of HLH (Helix-Loop-Helix) and four transmembrane segments were found in the amino acids. The sequence had been submitted to GenBank (Accession No.NM_-214157).展开更多
基金financially supported by Application Foundation Project in Sichuan Province(2009JY0061)Youth Fund Project of Educational Committee of Sichuan province(09ZB088)
文摘To explore the structural characteristic of ribosomal protein S29 (rpS29) gene of Ailuropoda melanoleuca (giant panda), primers were designed based on the known nucleotide sequence of rpS29 genes to clone the cDNA and genomic sequences of this gene from giant panda by RT-PCR and PCR strategy respectively, and then the cloned cDNA and genomic sequences were sequenced and analyzed preliminarily. The results indicated that the cDNA fragment of the rpS29 from the giant panda is 205bp in size in length, containing an open reading frame (ORF) of 171bp, encoding 56 amino acids. The length of the genomic sequence is 1 598 bp, with three exons and two introns. The coding sequence shows a high degree of homology to those of Homo sapiens, Bos taurus, Rattus norvegicus and Mus musculus with 75%, 94.74%, 89.47% and 88.89% respectively, While all the homologies for amino acid sequences are high up to 100%. Primary structure analysis revealed that the molecular weight of the putative rpS29 protein is 6.68 KD, with a theoretical pI of 10.63. Based on topology prediction, there are three distinct types of functional sites in the rpS29 protein of giant panda.
基金Item supported by national"973"program(No. 2004CB117500)national natural science foundation(No.30471237)
文摘Primers were designed according to the known sequences of Sterol Regulatory Element Binding Proteins-1c (SREBP-1c) genes of human, rat and pig. RT-PCR was then used to amplify porcine SREBP-1c gene with total RNA of spleen tissue. A 760 bp segment of cDNA was cloned and sequenced. Homogeneous comparison showed that the sequence of porcine SREBP-1c had 99.9% and 99.1% homogeneity with the two known partial mRNA sequences of porcine SREBP-1c gene. The complete cDNA was obtained mainly based on the known partial sequences, which has 3 830 bp, encoding 1 151 amino acids with a calculated molecular weight of 121 479 Da. It is the first time that we get complete encode sequence of porcine SREBP-1c gene. The complete cDNA sequence has high homogeneity with SREBP-1c gene of other species. A characteristic structure of HLH (Helix-Loop-Helix) and four transmembrane segments were found in the amino acids. The sequence had been submitted to GenBank (Accession No.NM_-214157).
