Natural variation is at the core of plant breeding. Genetic or linkage mapping is the traditional method for identifying loci/genes responsible for variati on in complex traits. More recently, association mapping or
We have isolated a recessive rice mutant, designated as indeterminate growth （ing）, which displays creeping and apparent heterochronic phenotypes in the vegetative period with lanky and winding culms. Rough mapping ...We have isolated a recessive rice mutant, designated as indeterminate growth （ing）, which displays creeping and apparent heterochronic phenotypes in the vegetative period with lanky and winding culms. Rough mapping and subsequent molecular characterization revealed that the ing mutant carries a large deletion, which corresponds to a 103 kb region in the Nipponbare genome, containing nine annotated genes on chromosome 3. Of these annotated genes, the SLR1 gene encoding a DELLA protein is the only one that is well characterized in its function, and its null mutation, which is caused by a single base deletion in the middle of the intronless SLR1 gene, confers a slender phenotype that bears close resemblance to the ing mutant phenotype. The primary cause of the ing mutant phenotype is the deletion of the SLR1 gene, and the ing mutant appears to be the first characterized mutant having the entire SLR1 sequence deleted. Our results also suggest that the deleted region of 103 kb does not contain an indispensable gene, whose dysfunction must result in a lethal phenotype.展开更多
文摘Natural variation is at the core of plant breeding. Genetic or linkage mapping is the traditional method for identifying loci/genes responsible for variati on in complex traits. More recently, association mapping or
基金supported by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences(PROBRAIN to S.I.) from Bio-oriented Technology Research Advancement Institution(BRAIN) in Japangrants from the Ministry of Education,Culture,Sports, Science,and Technology of Japan(No.17207002 to S.I.and 22780007 to K.T.)+1 种基金supported by the Global COE Program(to H.K.and S.I.)the NIBB Cooperative Research Program(9-151 to H.K.and 9-153 to M.M.),and the Graduate University for Advanced Studies(Sokendai)
文摘We have isolated a recessive rice mutant, designated as indeterminate growth （ing）, which displays creeping and apparent heterochronic phenotypes in the vegetative period with lanky and winding culms. Rough mapping and subsequent molecular characterization revealed that the ing mutant carries a large deletion, which corresponds to a 103 kb region in the Nipponbare genome, containing nine annotated genes on chromosome 3. Of these annotated genes, the SLR1 gene encoding a DELLA protein is the only one that is well characterized in its function, and its null mutation, which is caused by a single base deletion in the middle of the intronless SLR1 gene, confers a slender phenotype that bears close resemblance to the ing mutant phenotype. The primary cause of the ing mutant phenotype is the deletion of the SLR1 gene, and the ing mutant appears to be the first characterized mutant having the entire SLR1 sequence deleted. Our results also suggest that the deleted region of 103 kb does not contain an indispensable gene, whose dysfunction must result in a lethal phenotype.
