[Objective] The aim was to explore high-yielding cultivation techniques for forage sweet sorghum. [Method[ The effects of planting density and row spacing on plant productivity and grass yield of forage sweet sorghum ...[Objective] The aim was to explore high-yielding cultivation techniques for forage sweet sorghum. [Method[ The effects of planting density and row spacing on plant productivity and grass yield of forage sweet sorghum (Sorghum bicolor (L.) Moench) were compared using split-plot design and LSD method of IBMSPSSStatis- ticsv22. [Result]The planting density and row spacing had important influence on the plant productivity and yield of forage sweet sorghum. The optimum planting density- row spacing combination for plant productivity of forage sweet sorghum was A1B,, i. e., planting density of 75 000 plants/hm2 and row spacing of 40 cm, and the opti- mum combination for yield of forage sweet sorghum was A2B,, i.e., planting density of 225 000 plants/hm2 and row spacing of 40 cm. [Conclusion] This study will pro- vide theoretical basis and technical support for the production practice of forage sweet sorghum.展开更多
[ Objective] To explore the mixture sowing effects and optimal mixture ratio of alfalfa and orchard grass. [ Method] The alfalfa and orchard grass were sowed at different ratios (1 : 0, 3: 1, 1 : 1, 1 : 3 and 0...[ Objective] To explore the mixture sowing effects and optimal mixture ratio of alfalfa and orchard grass. [ Method] The alfalfa and orchard grass were sowed at different ratios (1 : 0, 3: 1, 1 : 1, 1 : 3 and 0:1 ). They were grouped and named as CK1, A1, A2, A3 and CK2., respectively. The forage yield, relative yield total (RYT) and the competition rate (CR) of alfalfa and orchard grass in each group were determined, respectively. [ Result] In the A3 treatment group, the total forage yield was the highest (4 827.67 kg/hm2) and significantly different from that of other treatment groups ( P 〈 0.01 ). In every treatment group, the RYT was higher than 1, and the alfalfa was more competitive than the orchard grass in the mixed-seeding pasture. [ Conclusion] Our studies may provide a basis for the establishment of high yield and high quality artificial mixture pasture.展开更多
Biosolids were applied with urea to produce a granulated organo-mineral fertiliser (OMF) for application by farm fertiliser equipment to a range of agricultural crops. The recommended rates of nitrogen, phosphate and ...Biosolids were applied with urea to produce a granulated organo-mineral fertiliser (OMF) for application by farm fertiliser equipment to a range of agricultural crops. The recommended rates of nitrogen, phosphate and potash were calculated for the test crops using “The Fertiliser Manual”, which assesses the nutrient requirement based on previous cropping, rainfall and soil index. The OMF produced similar crop yields compared to ammonium nitrate fertiliser when applied as a top-dressing to winter wheat, forage maize and grass cut for silage in the cropping years 2010 to 2014. In 2012 the grain yield of spring barley top-dressed with OMF was significantly lower than the conventional fertiliser treatment, due to dry conditions following application. For this reason it is recommended that OMF is incorporated into the seedbed for spring sown crops and The Safe Sludge Matrix guidelines followed. The experimental work presented shows that OMF can be used in sustainable crop production systems as a source of nitrogen and phosphorus for a range of agricultural crops.展开更多
Effects of transplanted seedling density and species on sward structure of native warm-season grass (NWSG) stands were compared in a randomized complete block design. About 6-week-old NWSG (big bluestem (BB, Andropogo...Effects of transplanted seedling density and species on sward structure of native warm-season grass (NWSG) stands were compared in a randomized complete block design. About 6-week-old NWSG (big bluestem (BB, Andropogon gerardii Vitman), eastern gamagrass (GG, Tripsacum dactyloides L.), indiangrass [IG, Sorghastrum nutans (L.) Nash] and switchgrass (SG, Panicum virgatum) seedlings were transplanted in 45-cm wide rows on clean-tilled seedbeds. Within-row spacing was 30, 25, or, 20 cm giving 10, 12, and 15 plants m-2 as low, medium, and high seedling density, respectively. During establishment, the stands were allowed uninterrupted first year growth without fertilizers or irrigation but when necessary, tall-growing broadleaf weeds were mechanically removed. In the following spring, all dead standing biomass was mowed down to allow emerging tillers access to sunlight. During the second year after planting, early-spring basal diameters, row-length intercepted by the NWSG crowns, mid-summer sward heights, and percentage bare ground were determined. From the second June after planting, and for two consecutive years, plots were harvested twice year-1 to assess forage biomass. Data showed that, unlike species, seedling density had no effect on the assessed parameters. Cumulative forage biomass, in kg DM ha-1, was the least for GG (4901) at low and the most (18,245) for SG at high seedling density during the second year. Corresponding values for the third year were 4500 and 7799 kg DM ha-1. Basal diameters ranged from 18 cm (BB) to 24 cm (IG) while percent row intercepts were from 6 (GG) to 46 (IG) with sward heights measuring 41 cm (IG) to 54 cm (GG). In each stand, percent ground cover by the NWSGs, and at every seedling density, averaged 60.5. Transplanting at ≥10 plant m-2 resulted in harvest-ready stands by the second year of establishment. And while close spacing favored the NWSGs against weeds, data showed that an initial plant density of >10 plants m-2 may not result in increased forage production worthy the additional establishment cost. Data on response to fertility management and forage quality attributes are necessary for more reliable practical recommendations.展开更多
[ Objectives ] The aim was to optimize the configuration of seedling density and line spacing of forage sweet sorghum ( Sorghum blcolor ( L. ) Moench) and explore its high-yield cultivation techniques. [ Methods] ...[ Objectives ] The aim was to optimize the configuration of seedling density and line spacing of forage sweet sorghum ( Sorghum blcolor ( L. ) Moench) and explore its high-yield cultivation techniques. [ Methods] Effects of such two influencing factors as line spacing and seedling density on the leaf-stem ratio, DW/FW ratio and grass yield of forage sweet sorghum were analyzed by using split-plot experiment design experiment method and LSD method of IBM. SPSS. Statis- tics. v22 stati, stics software. [ Results ~ Seedling density and line spacing had no obvious effect on the leaf-stem ratio and DW/FW ratio of forage sweet sorghum but had obvious influences on the grass yield. Moreover, the optimal combination of seedling density and line spacing for high yield of forage sweet sorghum was A2 B4, that is, seedling density was 225 000 plants/hm2, and line spacing was 40 cm. [ Conclusions] The results provided a theoretical basis and technical support for high-yield cultivation techniques of forage sweet sorghum.展开更多
Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated t...Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated the effects of inclusion of purple prairie clover(PPC,Dalea purpurea Vent.),a native legume forage,with native coolseason grasses on the in vitro fermentation and in situ digestibility of mixed forages.Methods:Whole plant PPC and mixtures of cool-season grasses were harvested when the PPC reached the vegetative(VEG),full flower(FL)and seedpod(SP)stages,and were combined in ratios(DM basis)of 0:100,25:75,50:50,75:25 and 100:0 at each maturity.In vitro ruminal incubations using these mixtures were conducted for 48 h to determine gas production(GP),in vitro DM disappearance(IVDMD),total volatile fatty acids(VFA)and ammonia-N production.Mixtures of forages harvested when the PPC reached the FL stage and 50:50 mixture of forages harvested at VEG,FL and SP stages were incubated in the rumen of three heifers for 0,2,6,12,24,48,72 and 96 h to determine in situ degradabilities of DM,neutral detergent fibre(aNDF)and crude protein(CP).Results:Contents of aNDF and ADF increased(P<0.01),while CP decreased(P<0.001)as PPC matured.Concentrations of extractable condensed tannins in PPC ranked as FL>VEG>SP(P<0.05).Regardless of PPC proportions in the mixture,GP decreased(P<0.05)with increasing PPC maturity.Increasing PPC proportions linearly increased(P<0.001)GP,IVDMD and total VFA at VEG,but linearly decreased(P<0.001)them at SP.Irrespective of PPC maturity,ammonia-N production linearly increased(P<0.01)with increasing proportions of PPC and the concentration was higher(P<0.05)at VEG than at FL and SP stages.Increasing proportion of PPC at either maturity linearly increased(P<0.001)molar percentage of acetate(A)and branched-chain VFA,but linearly decreased(P<0.001)molar percentage of propionate(P),resulting in a linearly increase(P<0.001)in the A:P ratio.Increasing FL PPC in the mixture linearly and quadratically(P<0.01)increased a(soluble fraction),but linearly and quadratically decreased(P<0.01)b(potentially degradable fraction)for DM and aNDF,resulting in linear(P<0.05)and quadratic(P<0.01)increases in DM and aNDF maximum potential degradabilities(a+b).Effective degradabilities of DM and aNDF were also linearly and quadratically increased(P<0.05),and CP was quadratically increased(P<0.05)with increasing FL PPC,with the greatest effective degradability being observed with ratios between 50:50 and 75:25.Ruminal maximum potential degradabilities of DM and aNDF decreased(P<0.001)as the forage matured.Effective degradability of DM ranked as VEG>FL>SP(P<0.001),whereas the effective degradability of aNDF was similar between VEG and FL and both were greater(P<0.01)than SP.Conclusions:Inclusion of vegetative PPC in a mixed forage diet resulted in the greatest digestibility and incorporation of PPC before seedpod stage with native grasses had a positive effect on ruminal fermentation.Effects of PPC on ruminal digestion depend on both the stage of maturity and its proportion in mixed legume-grass pastures.Pastures containing 50%of PPC in full flower stage would likely provide the greatest quality diet to grazing ruminants subject to potential animal selectivity.展开更多
The aim of this study was to identify the main classes of secondary metabolites present in the root and shoot crude extracts and fractions from the forage grass Urochloa humidicola (Rendle) Morrone & Zuloaga and t...The aim of this study was to identify the main classes of secondary metabolites present in the root and shoot crude extracts and fractions from the forage grass Urochloa humidicola (Rendle) Morrone & Zuloaga and to evaluate the allelopathic effect of these metabolites on forage legumes for intercropping. Phytochemical prospecting analyses, 1H NMR and capillary electrophoresis were performed on extracts of U. humidicola. Allelopathic activity was evaluated in germination of Stylosanthes, Macrotyloma axillare and Lactuca sativa L. (standard) in the presence of crude extracts, isolated saponins, flavonoids and trans-cinnamic acid. The metabolite classes present in the extracts could be determined by the combined use of the tested analytical techniques, but their use alone was usually not sufficient to chemically characterize the species. Capillary electrophoresis was effective in detecting phenolic compounds. Macrotyloma axillare was tolerant to crude extracts of U. humidicola. Saponins and trans-cinnamic acid, but not the flavonoids, reduced germination of the target plants.展开更多
Copper (Cu) mine tailings, because of their high content of heavy metals, are usually hostile to plant colonization. A pot experiment was conducted to determine the tolerance of four forage grasses to heavy metals i...Copper (Cu) mine tailings, because of their high content of heavy metals, are usually hostile to plant colonization. A pot experiment was conducted to determine the tolerance of four forage grasses to heavy metals in Cu mine tailings and to examine the variation in the microbial functional diversity of soils from the tailing sites in southern China. All the four grass species survived on Cu mine tailings and Cu mine tailing-soil mixture. However, on pure mine tailings, the growth was minimal, whereas the growth was maximum for the control without mine tailings. The tolerance of grasses to heavy metals followed the sequence: Paspalum notatum 〉 Festuea arundinaeea 〉 Lolium perenne 〉 Cynodon daetylon. The planting of forage grasses enhanced the soil microbial biomass. The Biolog data indicated that the soil microbial metabolic profile values (average well color development, community richness, and Shannon index) of the four forage grasses also followed the sequence: P. notatum 〉 F. arundinaeea 〉 L. perenne 〉 C. daetylon. Thus, P. notatum, under the experimental conditions of this study, may be considered as the preferred plant species for revegetation of Cu mine tailing areas.展开更多
Background: The habitat use and foraging behaviors of waterbirds are closely related to the distribution and abundance of their food resources. Reductions in food supply can cause waterbirds to shift their habitats an...Background: The habitat use and foraging behaviors of waterbirds are closely related to the distribution and abundance of their food resources. Reductions in food supply can cause waterbirds to shift their habitats and adjust their foraging behaviors to meet their nutritional requirements and increase fitness. Seasonal withdraw of the water levels in the river-connected lakes in the middle and lower Yangtze River floodplain provides abundant food resources for the wintering Greater White-fronted Goose(Anser albifrons). Sedge(Carex) meadows are critical foraging habitats for herbivorous waterbirds in the hydro-fluctuation belt, which changes with hydrological conditions and climate. This study aimed to examine the behavioral responses of the Greater White-fronted Goose to temporal-spatial changes of food availability in the Sedge meadows.Methods: Fields surveys were carried out at Shengjin Lake from November 2017 to April 2018. According to the phenology of Shengjin Lake, we divided the wintering season into three periods. The food density, minimum temperature, food items, grass height, and number of foraging geese were surveyed, and samples of the foraging behavior were collected. We analyzed the relationship of the foraging behavior and habitat use relative to the food resources, using correlation and linear regression analyses.Results: Along with the temporal-spatial variation and exploitation of food resources, the food abundance and items varied widely among the foraging sites. Over the whole wintering period, the foraging habitat with the highest utilization rate was the meadows, followed by the paddy fields, and then mudflats. Furthermore, the utilization of the meadows showed a bimodal distribution trend, while the paddy fields showed a unimodal trend, and a decreasing trend was seen in the mudflats over the whole wintering period. The results of the generalized linear model showed that the foraging rate was related to the food density and grass height, with a linearly increasing trend during the winter.Conclusions: With the change of food resources in the three habitats, the habitats used by the Greater White-fronted Geese shifted from meadows in the hydro-fluctuation belt to the paddy fields, and then back to the meadows. The time budget for foraging activities increased correspondingly, and there was an increase in the foraging rate to compensate for food shortages.展开更多
Two field experiments were conducted during two successive winter seasons of 2008/2009 and 2009/2010 to investigate the potentialities of mixing Egyptian clover with ryegrass under bio, organic and mineral fertilizati...Two field experiments were conducted during two successive winter seasons of 2008/2009 and 2009/2010 to investigate the potentialities of mixing Egyptian clover with ryegrass under bio, organic and mineral fertilization treatments and their combination to increase forage yield and quality grown under sandy soil conditions. The experiment included the combination of five mixing ratios (Egyptian clover alone, ryegrass alone, 75% Egyptian clover: 25% ryegrass, 50% Egyptian clover: 50% ryegrass and 25% Egyptian clover: 75% ryegrass) and eight fertilizer sources, which include control, organic fertilization, bio fertilization, chemical fertilizer, organic + bio fertilizer, organic + chemical fertilizer, bio + chemical fertilizer and combination of organic and chemical and bio fertilizers. The obtained results indicate the superiority of 75% E. clover: 25% ryegrass mixture fertilizedby Bio + O + N in fresh and dry forage production. On the other hand, it reported the lowest dry weight of weeds g/m2. Chemical analysis of forage plants showed that the mixture of ?75% E. clover: 25% ryegrass surpassed that of other treatments yield for crude protein, ether extract and ash. The results also revealed that the highest record of DCP, crude fiber and TDNY was obtained by forage mixture of 75% E. clover: 25% ryegrass fertilized with Bio + O + N. Such higher yield of these characters hassecured a balanced ratio which is really needed for ruminants ration.展开更多
This study aimed to screen suitable species of Pennisetum purpureum for planting in northern Guangxi Zhuang Autonomous Region. During 2012-2014, a comparative field trial was carried out in Lingyun County of Baise Cit...This study aimed to screen suitable species of Pennisetum purpureum for planting in northern Guangxi Zhuang Autonomous Region. During 2012-2014, a comparative field trial was carried out in Lingyun County of Baise City in northern Guangxi to compare plant height, yield, stem-leaf ratio and nutrient composition am-ong Pennisetum purpureum Schumab cv. Purple, P. purpureum Schum cv. Guimin and P. purpureum Rich &#215; P. americana King grass cv. Reyan No.4. The results in-dicated that average annual fresh yield of P. purpureum Schumab cv. Purple reached 141.4 t/hm2, which was 2.3 t/hm^2 higher than that of P. purpureum_Rich×P. americana_King grass cv. Reyan No.4; both average annual fresh yield of these two species was lower than that of P. purpureum Schum cv. Guimin. Stem-leaf ratio of P. purpureum Schumab cv. Purple was the lowest (1.17), which was 17.8% lower compared with P. purpureum Schum cv. Guimin, with significant differences (P〈0.05), indicating that P. purpureum Schumab cv. Purple had more leaves. Crude protein content of P. purpureum_Schumab cv. Purple was slightly lower than that of P. purpureum Rich×P. americana_King grass cv. Reyan No.4, while P. purpureum Schumab cv. Purple was superior to P. purpureum Schum cv. Guimin and P. pur-pureum Rich ×P. americana_King grass cv. Reyan No.4 in other indicators. Specifi-cal y, P. purpureum Schumab cv. Purple contained 26.6% of dry matter, 39.1% of nitrogen-free extract and 2.9% of crude fat.展开更多
【目的】牧草太空育种是近三十年来新兴的一种育种技术,具有变异多、变幅大、稳定快等特点,可缩短牧草育种周期。本文综述了太空育种的研究现状,分析总结了太空育种的原理、方法及可能存在的问题,以期为今后牧草太空育种提供更多参考和...【目的】牧草太空育种是近三十年来新兴的一种育种技术,具有变异多、变幅大、稳定快等特点,可缩短牧草育种周期。本文综述了太空育种的研究现状,分析总结了太空育种的原理、方法及可能存在的问题,以期为今后牧草太空育种提供更多参考和启发。【方法】通过Web of Science、Google Scholar、中国知网、百度学术等网站查阅了大量太空育种尤其是牧草太空育种的相关文献,并对文献资料进行收集、分析和整理。【结果】太空环境诱变是当今牧草育种重要发展方向之一,通过总结太空诱变技术在牧草育种中的应用,分析了牧草太空育种中面临的问题和挑战,展望了采用太空环境诱变创制牧草新品种的前景。【结论】太空环境诱变能诱发牧草基因组中的突变,增加其遗传多样性,从而创造出具有新特性的植物品种,对牧草类作物进行种质资源的创制具有重大意义,同时也为快速培育优良牧草新品种及特异种质资源开辟了一条新途径。太空育种技术为牧草育种开辟了新的可能性,有望成为未来太空农业的重要组成部分。展开更多
文摘[Objective] The aim was to explore high-yielding cultivation techniques for forage sweet sorghum. [Method[ The effects of planting density and row spacing on plant productivity and grass yield of forage sweet sorghum (Sorghum bicolor (L.) Moench) were compared using split-plot design and LSD method of IBMSPSSStatis- ticsv22. [Result]The planting density and row spacing had important influence on the plant productivity and yield of forage sweet sorghum. The optimum planting density- row spacing combination for plant productivity of forage sweet sorghum was A1B,, i. e., planting density of 75 000 plants/hm2 and row spacing of 40 cm, and the opti- mum combination for yield of forage sweet sorghum was A2B,, i.e., planting density of 225 000 plants/hm2 and row spacing of 40 cm. [Conclusion] This study will pro- vide theoretical basis and technical support for the production practice of forage sweet sorghum.
基金supported by the Youth Fund of Sichuan Academy of Agricultural Sciences ( 2009QNJJ-027)
文摘[ Objective] To explore the mixture sowing effects and optimal mixture ratio of alfalfa and orchard grass. [ Method] The alfalfa and orchard grass were sowed at different ratios (1 : 0, 3: 1, 1 : 1, 1 : 3 and 0:1 ). They were grouped and named as CK1, A1, A2, A3 and CK2., respectively. The forage yield, relative yield total (RYT) and the competition rate (CR) of alfalfa and orchard grass in each group were determined, respectively. [ Result] In the A3 treatment group, the total forage yield was the highest (4 827.67 kg/hm2) and significantly different from that of other treatment groups ( P 〈 0.01 ). In every treatment group, the RYT was higher than 1, and the alfalfa was more competitive than the orchard grass in the mixed-seeding pasture. [ Conclusion] Our studies may provide a basis for the establishment of high yield and high quality artificial mixture pasture.
文摘Biosolids were applied with urea to produce a granulated organo-mineral fertiliser (OMF) for application by farm fertiliser equipment to a range of agricultural crops. The recommended rates of nitrogen, phosphate and potash were calculated for the test crops using “The Fertiliser Manual”, which assesses the nutrient requirement based on previous cropping, rainfall and soil index. The OMF produced similar crop yields compared to ammonium nitrate fertiliser when applied as a top-dressing to winter wheat, forage maize and grass cut for silage in the cropping years 2010 to 2014. In 2012 the grain yield of spring barley top-dressed with OMF was significantly lower than the conventional fertiliser treatment, due to dry conditions following application. For this reason it is recommended that OMF is incorporated into the seedbed for spring sown crops and The Safe Sludge Matrix guidelines followed. The experimental work presented shows that OMF can be used in sustainable crop production systems as a source of nitrogen and phosphorus for a range of agricultural crops.
文摘Effects of transplanted seedling density and species on sward structure of native warm-season grass (NWSG) stands were compared in a randomized complete block design. About 6-week-old NWSG (big bluestem (BB, Andropogon gerardii Vitman), eastern gamagrass (GG, Tripsacum dactyloides L.), indiangrass [IG, Sorghastrum nutans (L.) Nash] and switchgrass (SG, Panicum virgatum) seedlings were transplanted in 45-cm wide rows on clean-tilled seedbeds. Within-row spacing was 30, 25, or, 20 cm giving 10, 12, and 15 plants m-2 as low, medium, and high seedling density, respectively. During establishment, the stands were allowed uninterrupted first year growth without fertilizers or irrigation but when necessary, tall-growing broadleaf weeds were mechanically removed. In the following spring, all dead standing biomass was mowed down to allow emerging tillers access to sunlight. During the second year after planting, early-spring basal diameters, row-length intercepted by the NWSG crowns, mid-summer sward heights, and percentage bare ground were determined. From the second June after planting, and for two consecutive years, plots were harvested twice year-1 to assess forage biomass. Data showed that, unlike species, seedling density had no effect on the assessed parameters. Cumulative forage biomass, in kg DM ha-1, was the least for GG (4901) at low and the most (18,245) for SG at high seedling density during the second year. Corresponding values for the third year were 4500 and 7799 kg DM ha-1. Basal diameters ranged from 18 cm (BB) to 24 cm (IG) while percent row intercepts were from 6 (GG) to 46 (IG) with sward heights measuring 41 cm (IG) to 54 cm (GG). In each stand, percent ground cover by the NWSGs, and at every seedling density, averaged 60.5. Transplanting at ≥10 plant m-2 resulted in harvest-ready stands by the second year of establishment. And while close spacing favored the NWSGs against weeds, data showed that an initial plant density of >10 plants m-2 may not result in increased forage production worthy the additional establishment cost. Data on response to fertility management and forage quality attributes are necessary for more reliable practical recommendations.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(20120304201)
文摘[ Objectives ] The aim was to optimize the configuration of seedling density and line spacing of forage sweet sorghum ( Sorghum blcolor ( L. ) Moench) and explore its high-yield cultivation techniques. [ Methods] Effects of such two influencing factors as line spacing and seedling density on the leaf-stem ratio, DW/FW ratio and grass yield of forage sweet sorghum were analyzed by using split-plot experiment design experiment method and LSD method of IBM. SPSS. Statis- tics. v22 stati, stics software. [ Results ~ Seedling density and line spacing had no obvious effect on the leaf-stem ratio and DW/FW ratio of forage sweet sorghum but had obvious influences on the grass yield. Moreover, the optimal combination of seedling density and line spacing for high yield of forage sweet sorghum was A2 B4, that is, seedling density was 225 000 plants/hm2, and line spacing was 40 cm. [ Conclusions] The results provided a theoretical basis and technical support for high-yield cultivation techniques of forage sweet sorghum.
基金This study was funded from AAFC/Canadian Beef Cattle Industry Science ClusterAlberta Livestock and Meat Agency.This is Lethbridge Research and Development Centre contribution number 38717038.
文摘Background:Incorporation of legume species into native North American pastures is considered an effective method to increase native pasture productivity and improve the nutritive value of forage.This study evaluated the effects of inclusion of purple prairie clover(PPC,Dalea purpurea Vent.),a native legume forage,with native coolseason grasses on the in vitro fermentation and in situ digestibility of mixed forages.Methods:Whole plant PPC and mixtures of cool-season grasses were harvested when the PPC reached the vegetative(VEG),full flower(FL)and seedpod(SP)stages,and were combined in ratios(DM basis)of 0:100,25:75,50:50,75:25 and 100:0 at each maturity.In vitro ruminal incubations using these mixtures were conducted for 48 h to determine gas production(GP),in vitro DM disappearance(IVDMD),total volatile fatty acids(VFA)and ammonia-N production.Mixtures of forages harvested when the PPC reached the FL stage and 50:50 mixture of forages harvested at VEG,FL and SP stages were incubated in the rumen of three heifers for 0,2,6,12,24,48,72 and 96 h to determine in situ degradabilities of DM,neutral detergent fibre(aNDF)and crude protein(CP).Results:Contents of aNDF and ADF increased(P<0.01),while CP decreased(P<0.001)as PPC matured.Concentrations of extractable condensed tannins in PPC ranked as FL>VEG>SP(P<0.05).Regardless of PPC proportions in the mixture,GP decreased(P<0.05)with increasing PPC maturity.Increasing PPC proportions linearly increased(P<0.001)GP,IVDMD and total VFA at VEG,but linearly decreased(P<0.001)them at SP.Irrespective of PPC maturity,ammonia-N production linearly increased(P<0.01)with increasing proportions of PPC and the concentration was higher(P<0.05)at VEG than at FL and SP stages.Increasing proportion of PPC at either maturity linearly increased(P<0.001)molar percentage of acetate(A)and branched-chain VFA,but linearly decreased(P<0.001)molar percentage of propionate(P),resulting in a linearly increase(P<0.001)in the A:P ratio.Increasing FL PPC in the mixture linearly and quadratically(P<0.01)increased a(soluble fraction),but linearly and quadratically decreased(P<0.01)b(potentially degradable fraction)for DM and aNDF,resulting in linear(P<0.05)and quadratic(P<0.01)increases in DM and aNDF maximum potential degradabilities(a+b).Effective degradabilities of DM and aNDF were also linearly and quadratically increased(P<0.05),and CP was quadratically increased(P<0.05)with increasing FL PPC,with the greatest effective degradability being observed with ratios between 50:50 and 75:25.Ruminal maximum potential degradabilities of DM and aNDF decreased(P<0.001)as the forage matured.Effective degradability of DM ranked as VEG>FL>SP(P<0.001),whereas the effective degradability of aNDF was similar between VEG and FL and both were greater(P<0.01)than SP.Conclusions:Inclusion of vegetative PPC in a mixed forage diet resulted in the greatest digestibility and incorporation of PPC before seedpod stage with native grasses had a positive effect on ruminal fermentation.Effects of PPC on ruminal digestion depend on both the stage of maturity and its proportion in mixed legume-grass pastures.Pastures containing 50%of PPC in full flower stage would likely provide the greatest quality diet to grazing ruminants subject to potential animal selectivity.
文摘The aim of this study was to identify the main classes of secondary metabolites present in the root and shoot crude extracts and fractions from the forage grass Urochloa humidicola (Rendle) Morrone & Zuloaga and to evaluate the allelopathic effect of these metabolites on forage legumes for intercropping. Phytochemical prospecting analyses, 1H NMR and capillary electrophoresis were performed on extracts of U. humidicola. Allelopathic activity was evaluated in germination of Stylosanthes, Macrotyloma axillare and Lactuca sativa L. (standard) in the presence of crude extracts, isolated saponins, flavonoids and trans-cinnamic acid. The metabolite classes present in the extracts could be determined by the combined use of the tested analytical techniques, but their use alone was usually not sufficient to chemically characterize the species. Capillary electrophoresis was effective in detecting phenolic compounds. Macrotyloma axillare was tolerant to crude extracts of U. humidicola. Saponins and trans-cinnamic acid, but not the flavonoids, reduced germination of the target plants.
基金the National Natural Sciences Foundation of China (Nos40171054 and 40125005)the National Key Basic Research Support Foundation of China (No2002CB410809/10)
文摘Copper (Cu) mine tailings, because of their high content of heavy metals, are usually hostile to plant colonization. A pot experiment was conducted to determine the tolerance of four forage grasses to heavy metals in Cu mine tailings and to examine the variation in the microbial functional diversity of soils from the tailing sites in southern China. All the four grass species survived on Cu mine tailings and Cu mine tailing-soil mixture. However, on pure mine tailings, the growth was minimal, whereas the growth was maximum for the control without mine tailings. The tolerance of grasses to heavy metals followed the sequence: Paspalum notatum 〉 Festuea arundinaeea 〉 Lolium perenne 〉 Cynodon daetylon. The planting of forage grasses enhanced the soil microbial biomass. The Biolog data indicated that the soil microbial metabolic profile values (average well color development, community richness, and Shannon index) of the four forage grasses also followed the sequence: P. notatum 〉 F. arundinaeea 〉 L. perenne 〉 C. daetylon. Thus, P. notatum, under the experimental conditions of this study, may be considered as the preferred plant species for revegetation of Cu mine tailing areas.
基金the National Natural Science Foundation of China(Grant No.31472020).
文摘Background: The habitat use and foraging behaviors of waterbirds are closely related to the distribution and abundance of their food resources. Reductions in food supply can cause waterbirds to shift their habitats and adjust their foraging behaviors to meet their nutritional requirements and increase fitness. Seasonal withdraw of the water levels in the river-connected lakes in the middle and lower Yangtze River floodplain provides abundant food resources for the wintering Greater White-fronted Goose(Anser albifrons). Sedge(Carex) meadows are critical foraging habitats for herbivorous waterbirds in the hydro-fluctuation belt, which changes with hydrological conditions and climate. This study aimed to examine the behavioral responses of the Greater White-fronted Goose to temporal-spatial changes of food availability in the Sedge meadows.Methods: Fields surveys were carried out at Shengjin Lake from November 2017 to April 2018. According to the phenology of Shengjin Lake, we divided the wintering season into three periods. The food density, minimum temperature, food items, grass height, and number of foraging geese were surveyed, and samples of the foraging behavior were collected. We analyzed the relationship of the foraging behavior and habitat use relative to the food resources, using correlation and linear regression analyses.Results: Along with the temporal-spatial variation and exploitation of food resources, the food abundance and items varied widely among the foraging sites. Over the whole wintering period, the foraging habitat with the highest utilization rate was the meadows, followed by the paddy fields, and then mudflats. Furthermore, the utilization of the meadows showed a bimodal distribution trend, while the paddy fields showed a unimodal trend, and a decreasing trend was seen in the mudflats over the whole wintering period. The results of the generalized linear model showed that the foraging rate was related to the food density and grass height, with a linearly increasing trend during the winter.Conclusions: With the change of food resources in the three habitats, the habitats used by the Greater White-fronted Geese shifted from meadows in the hydro-fluctuation belt to the paddy fields, and then back to the meadows. The time budget for foraging activities increased correspondingly, and there was an increase in the foraging rate to compensate for food shortages.
文摘Two field experiments were conducted during two successive winter seasons of 2008/2009 and 2009/2010 to investigate the potentialities of mixing Egyptian clover with ryegrass under bio, organic and mineral fertilization treatments and their combination to increase forage yield and quality grown under sandy soil conditions. The experiment included the combination of five mixing ratios (Egyptian clover alone, ryegrass alone, 75% Egyptian clover: 25% ryegrass, 50% Egyptian clover: 50% ryegrass and 25% Egyptian clover: 75% ryegrass) and eight fertilizer sources, which include control, organic fertilization, bio fertilization, chemical fertilizer, organic + bio fertilizer, organic + chemical fertilizer, bio + chemical fertilizer and combination of organic and chemical and bio fertilizers. The obtained results indicate the superiority of 75% E. clover: 25% ryegrass mixture fertilizedby Bio + O + N in fresh and dry forage production. On the other hand, it reported the lowest dry weight of weeds g/m2. Chemical analysis of forage plants showed that the mixture of ?75% E. clover: 25% ryegrass surpassed that of other treatments yield for crude protein, ether extract and ash. The results also revealed that the highest record of DCP, crude fiber and TDNY was obtained by forage mixture of 75% E. clover: 25% ryegrass fertilized with Bio + O + N. Such higher yield of these characters hassecured a balanced ratio which is really needed for ruminants ration.
基金Supported by Major Project of Science and Technology in Guangxi Zhuang Autonomous Region(GK AA16380026)Special Fund for Innovation Team Building in Guangxi Zhuang Autonomous Region from Modern Agricultural Industry Technology System of China(nycytxgxcxtd-09-04)Project of Processing and Application Demonstration of High-yielding Forage Grass Products in South China(GYMK 201633019)~~
文摘This study aimed to screen suitable species of Pennisetum purpureum for planting in northern Guangxi Zhuang Autonomous Region. During 2012-2014, a comparative field trial was carried out in Lingyun County of Baise City in northern Guangxi to compare plant height, yield, stem-leaf ratio and nutrient composition am-ong Pennisetum purpureum Schumab cv. Purple, P. purpureum Schum cv. Guimin and P. purpureum Rich &#215; P. americana King grass cv. Reyan No.4. The results in-dicated that average annual fresh yield of P. purpureum Schumab cv. Purple reached 141.4 t/hm2, which was 2.3 t/hm^2 higher than that of P. purpureum_Rich×P. americana_King grass cv. Reyan No.4; both average annual fresh yield of these two species was lower than that of P. purpureum Schum cv. Guimin. Stem-leaf ratio of P. purpureum Schumab cv. Purple was the lowest (1.17), which was 17.8% lower compared with P. purpureum Schum cv. Guimin, with significant differences (P〈0.05), indicating that P. purpureum Schumab cv. Purple had more leaves. Crude protein content of P. purpureum_Schumab cv. Purple was slightly lower than that of P. purpureum Rich×P. americana_King grass cv. Reyan No.4, while P. purpureum Schumab cv. Purple was superior to P. purpureum Schum cv. Guimin and P. pur-pureum Rich ×P. americana_King grass cv. Reyan No.4 in other indicators. Specifi-cal y, P. purpureum Schumab cv. Purple contained 26.6% of dry matter, 39.1% of nitrogen-free extract and 2.9% of crude fat.
文摘【目的】牧草太空育种是近三十年来新兴的一种育种技术,具有变异多、变幅大、稳定快等特点,可缩短牧草育种周期。本文综述了太空育种的研究现状,分析总结了太空育种的原理、方法及可能存在的问题,以期为今后牧草太空育种提供更多参考和启发。【方法】通过Web of Science、Google Scholar、中国知网、百度学术等网站查阅了大量太空育种尤其是牧草太空育种的相关文献,并对文献资料进行收集、分析和整理。【结果】太空环境诱变是当今牧草育种重要发展方向之一,通过总结太空诱变技术在牧草育种中的应用,分析了牧草太空育种中面临的问题和挑战,展望了采用太空环境诱变创制牧草新品种的前景。【结论】太空环境诱变能诱发牧草基因组中的突变,增加其遗传多样性,从而创造出具有新特性的植物品种,对牧草类作物进行种质资源的创制具有重大意义,同时也为快速培育优良牧草新品种及特异种质资源开辟了一条新途径。太空育种技术为牧草育种开辟了新的可能性,有望成为未来太空农业的重要组成部分。