In order to study the ability of bentonites to remove heavy metal ions from waste water and its factors affecting it,batch sorption experiments of Cu2+ were conducted on Ca-bentonite and Na-bentonite under various con...In order to study the ability of bentonites to remove heavy metal ions from waste water and its factors affecting it,batch sorption experiments of Cu2+ were conducted on Ca-bentonite and Na-bentonite under various conditions.The results show that the adsorption process of bentonite accorded with the Freundlich isotherm model and that the sorption results of Na-bentonite are better than those of Ca-bentonite.Adsorption behavior of both bentonites was strongly depending on pH,initial concentration and additional amounts of bentonites.There are three kinds of adsorption mechanism at different ranges of pH values:the competition adsorption between Cu2+ and H+(pH<3),ion-exchange adsorption(pH=3～7) and precipitation adsorption of copper hydroxyl compounds(pH>8.3).The removal rate of bentonite decreases with an increase in the initial metal ion concentration.The maximum adsorption capacity of Na-bentonite was 26 mg/g and that of Ca-bentonite 12 mg/g.The removal rate of Cu2+ was practically 100% at the initial concentration of 40 mg/L,when 4 g/L of Na-bentonite and 14 g/L of Ca-bentonite were added to the solution.展开更多
AIM: To study the effect of copper transporting P-type ATPase in copper metabolism of hepatocyte and pathogenesis of Wilson disease (WD).METHODS: WD copper transporting properties in some organelles of the cultu...AIM: To study the effect of copper transporting P-type ATPase in copper metabolism of hepatocyte and pathogenesis of Wilson disease (WD).METHODS: WD copper transporting properties in some organelles of the cultured hepatocytes were studied from WD patients and normal controls. These cultured hepatocytes were incubated in the media of copper 15mg.L-1 only, copper 15 mg. L-1 with vincristine (agonist of P-type ArPase) 0.5mg. L-1, or copper 15 mg. L-1 withvanadate (antagonist of P-type ATPase) 18.39 mg. L-1separately. Microsome (endoplasmic reticulum and Golgi apparatus), lysosome, mitochondria, and cytosol were isolated by differential centrifugation. Copper contents in these organelles were measured with atomic absorption spectrophotometer, and the influence in copper transportion of these organelles by vanadate and vincristine were comparatively analyzed between WD patients and controls.WD copper transporting P-type ATPase was detected by SDS-PAGE in conjunction with Western blot in liver samples of WD patients and controls.RESULTS: The specific WD proteins (Mr155 000 lanes) were expressed in human hepatocytes, including the control and WD patients. After incubation with medium containing copper for 2 h or 24 h, the microsome copper concentration in WD patients was obviously lower than that of controls,and the addtion of vanadate or vincristine would change the copper transporting of microsomes obviously. When incubated with vincristine, levels of copper in microsome were significantly increased, while incubated with vanadate,the copper concentrations in microsome were obviously decreased. The results indicated that there were Wdproteins, the copper transportion P-type ATPase in the microsome of hepatocytes. WD patients possessed abnormal copper transporting function of WD protein in the microsome, and the agonist might correct the defect of copper transportion by promoting the activity of copper transportion P-type ATPase.CONCLUSION: Copper transportion P-type ATPase plays an important role in hepatocytic copper metabolism.Dysfunction of hepatocytic WD protein copper transportion might be one of the most important factors for WD.展开更多
基金Projects D2007000695 and D2009000833 supported by the Natural Science Foundation of Hebei Province, China
文摘In order to study the ability of bentonites to remove heavy metal ions from waste water and its factors affecting it,batch sorption experiments of Cu2+ were conducted on Ca-bentonite and Na-bentonite under various conditions.The results show that the adsorption process of bentonite accorded with the Freundlich isotherm model and that the sorption results of Na-bentonite are better than those of Ca-bentonite.Adsorption behavior of both bentonites was strongly depending on pH,initial concentration and additional amounts of bentonites.There are three kinds of adsorption mechanism at different ranges of pH values:the competition adsorption between Cu2+ and H+(pH<3),ion-exchange adsorption(pH=3～7) and precipitation adsorption of copper hydroxyl compounds(pH>8.3).The removal rate of bentonite decreases with an increase in the initial metal ion concentration.The maximum adsorption capacity of Na-bentonite was 26 mg/g and that of Ca-bentonite 12 mg/g.The removal rate of Cu2+ was practically 100% at the initial concentration of 40 mg/L,when 4 g/L of Na-bentonite and 14 g/L of Ca-bentonite were added to the solution.
基金Supported by Key Clinical Program of Ministry of Ministry of Health(No.37091)"211 Project"of SUMS sponsored by Ministry of Health and Guangdong Provincial Natural Science Foundation,No.990064
文摘AIM: To study the effect of copper transporting P-type ATPase in copper metabolism of hepatocyte and pathogenesis of Wilson disease (WD).METHODS: WD copper transporting properties in some organelles of the cultured hepatocytes were studied from WD patients and normal controls. These cultured hepatocytes were incubated in the media of copper 15mg.L-1 only, copper 15 mg. L-1 with vincristine (agonist of P-type ArPase) 0.5mg. L-1, or copper 15 mg. L-1 withvanadate (antagonist of P-type ATPase) 18.39 mg. L-1separately. Microsome (endoplasmic reticulum and Golgi apparatus), lysosome, mitochondria, and cytosol were isolated by differential centrifugation. Copper contents in these organelles were measured with atomic absorption spectrophotometer, and the influence in copper transportion of these organelles by vanadate and vincristine were comparatively analyzed between WD patients and controls.WD copper transporting P-type ATPase was detected by SDS-PAGE in conjunction with Western blot in liver samples of WD patients and controls.RESULTS: The specific WD proteins (Mr155 000 lanes) were expressed in human hepatocytes, including the control and WD patients. After incubation with medium containing copper for 2 h or 24 h, the microsome copper concentration in WD patients was obviously lower than that of controls,and the addtion of vanadate or vincristine would change the copper transporting of microsomes obviously. When incubated with vincristine, levels of copper in microsome were significantly increased, while incubated with vanadate,the copper concentrations in microsome were obviously decreased. The results indicated that there were Wdproteins, the copper transportion P-type ATPase in the microsome of hepatocytes. WD patients possessed abnormal copper transporting function of WD protein in the microsome, and the agonist might correct the defect of copper transportion by promoting the activity of copper transportion P-type ATPase.CONCLUSION: Copper transportion P-type ATPase plays an important role in hepatocytic copper metabolism.Dysfunction of hepatocytic WD protein copper transportion might be one of the most important factors for WD.
