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Cloning and Expressing of a Gene Encoding Cytosolic Copper/Zinc Superoxide Dismutase in the Upland Cotton
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作者 HU Gen-hai YU Shu-xun +1 位作者 FAN Shu-li SONG Mei-zhen 《Agricultural Sciences in China》 CAS CSCD 2007年第5期536-544,共9页
In this study, a gene encoding a superoxide dismutase (SOD) was cloned from senescent leaves of cotton (Gossypium hirsutum), and its expressing profile was analyzed. The gene was cloned by rapid amplification of c... In this study, a gene encoding a superoxide dismutase (SOD) was cloned from senescent leaves of cotton (Gossypium hirsutum), and its expressing profile was analyzed. The gene was cloned by rapid amplification of cDNA ends (RACE) method. Northern blotting was used to show the profile of the gene expression, and the enzyme activity was mensurated by NBT deoxidization method in different growth periods. The full length of a gene of cytosolic copper/zinc superoxide dismutase (Cu/Zn-SOD) was isolated from cotton (GenBank Accession Number: DQ445093). The sequence of cDNA contained 682 bp, the opening reading frame 456 bp, and encoded polypeptide 152 amino acids with the predicted molecular mass of 15.03 kD and theoretical pI of 6.09. The amino acid sequence was similar with the other plants from 82 to 87%. Southern blotting showed that the gene had different number of copies in different cotton species. Northern blotting suggested that the gene had different expression in different tissues and development stages. The enzyme activity was the highest in peak flowering stage. The cotton cytosolic (Cu/Zn-SOD) had lower copies in the upland cotton. The copper/zinc superoxide dismutase mRNA expressing level showed regular changing in the whole development stages; it was lower in the former stages, higher in latter stages and the highest at the peak flowering stage. The curve of the copper/zinc superoxide dismutase mRNA expressing level was consistent with that of the Cu/Zn-SOD enzyme activity. The copper/zinc superoxide dismutase mRNA expressing levels of different organs showed that the gene was higher in the root, leaf, and lower in the flower. 展开更多
关键词 cotton copper/zinc superoxide dismutase GENE CLONING
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INFLUENCE OF ZINC,MANGANESE AND SELENIUM ONSUPEROXIDE DISMUTASE ACTIVITY IN LUNGCANCER TISSUE AND CELL IN CULTURE 被引量:1
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作者 喻伦银 夏东 刘汉桥 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1996年第1期42-45,共4页
In this experiment,the cancer tissues and cells,Which were derived from Lewis lung cancer and A549 lung Cancer cell line,were respectively divided into four groups and zinc, manganese and selenium were respectively ad... In this experiment,the cancer tissues and cells,Which were derived from Lewis lung cancer and A549 lung Cancer cell line,were respectively divided into four groups and zinc, manganese and selenium were respectively added to the medium for 24 hours. The superoxide dismutase activity in the tissues and the cells was estimated. It was found that the SOD activity was enhanced by zinc and manganese and the effect of zinc on SOD activity was superior to that of manganese. We supposed that the enhance of the SOD activity was relative to the activation of the SOD apoenzymes. This experimental result indicated that the inhibitory effect of zinc and manganese on carcinogenesis was achieved by SOD and the elements might be considered a SOD activator. 展开更多
关键词 superoxide dismutase zinc Manganese Selenium Lung cancer TISSUE Cell line Culture.
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Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis 被引量:2
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作者 WANG Hui YANG Hongshuai +2 位作者 LIU Jiahui LI Yanhong LIU Zhigang 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2016年第4期51-57,共7页
Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainl... Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD(SOD1) and Mn-SOD(SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature(18–34°C) and copper ion concentration(0.1–1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant(P〈0.01), the quadratic effect of temperature was significant(P〈0.05); the linear effect of copper ion concentration was not significant(P〉0.05), while the quadratic effect of copper ion concentration was highly significant(P〈0.01); the interactive effect of temperature and copper ion concentration was not significant(P〉0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator. 展开更多
关键词 Crassostrea ariakensis superoxide dismutase temperature copper ion concentration combined effect
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Copper Delivery by the Copper Chaperone for Chloroplast and Cytosolic Copper/Zinc- Superoxide Dismutases: Regulation and Unexpected Phenotypes in an Arabidopsis Mutant 被引量:10
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作者 Christopher M. Cohu Salah E. Abdel-Ghany +5 位作者 Kathryn A. Gogolin Reynolds Alexander M. Onofrio Jared R. Bodecker Jeffrey A. Kimbrel Krishna K. Niyogi Marinus Pilon 《Molecular Plant》 SCIE CAS CSCD 2009年第6期1336-1350,共15页
Copper (Cu) is an important mineral nutrient found in chloroplasts as a cofactor associated with plastocyanin and Cu/Zn superoxide dismutase (Cu/ZnSOD). Superoxide dismutases are metallo-enzymes found in most oxyg... Copper (Cu) is an important mineral nutrient found in chloroplasts as a cofactor associated with plastocyanin and Cu/Zn superoxide dismutase (Cu/ZnSOD). Superoxide dismutases are metallo-enzymes found in most oxygenic organisms with proposed roles in reducing oxidative stress. Several recent studies in Arabidopsis have shown that microRNAs and a SQUAMOSA promoter binding protein-like7 (SPL7) transcription factor function to down-regulate the expression of many Cu-proteins, including Cu/ZnSOD in both plastids and the cytosol, during growth on low Cu. Plants contain the Cu Chaperone for SOD (CCS) that delivers Cu to Cu/ZnSODs, and, in Arabidopsis, both cytosolic and plastidic CCS versions are encoded by one gene. In this study, we demonstrate that Arabiclopsis CCS transcript levels are regulated by Cu, mediated by microRNA 398 that was not previously predicted to target CCS. The microRNA target site is conserved in CCS of Oryza sativa. The data suggest that Cu-regulated microRNAs may have more mRNA targets than was previously predicted. A CCS null mutant has no measurable SOD activity in the chloroplast and cytosol, indicating an absolute requirement for CCS. When the CCS null mutant was grown on high Cu media, it lacked both Fe superoxide dismutase (FeSOD) and Cu/ZnSOD activity. However, this did not lead to a visual phenotype and no photosynthetic deficiencies were detected, even after high light stress. These results indicate that Cu/ZnSOD is not a pivotal component of the photosynthetic anti-oxidant system during growth in laboratory conditions. 展开更多
关键词 CHLOROPLAST PHOTOSYNTHESIS copper homeostasis superoxide dismutase microRNA.
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Antioxidant role of zinc in diabetes mellitus 被引量:4
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作者 Kyria Jayanne Clímaco Cruz Ana Raquel Soares de Oliveira Dilina do Nascimento Marreiro 《World Journal of Diabetes》 SCIE CAS 2015年第2期333-337,共5页
Chronic hyperglycemia statue noticed in diabetes mellitus favors the manifestation of oxidative stress by increasing the production of reactive oxygen species and/or by reducing the antioxidant defense system activity... Chronic hyperglycemia statue noticed in diabetes mellitus favors the manifestation of oxidative stress by increasing the production of reactive oxygen species and/or by reducing the antioxidant defense system activity.Zinc plays an important role in antioxidant defense in type2 diabetic patients by notably acting as a cofactor of the superoxide dismutase enzyme,by modulating the glutathione metabolism and metallothionein expression,by competing with iron and copper in the cell membrane and by inhibiting nicotinamide adenine dinucleotide phosphate-oxidase enzyme.Zinc also improves the oxidative stress in these patients by reducing chronic hyperglycemia.It indeed promotes phosphorylation of insulin receptors by enhancing transport of glucose into cells.However,several studies reveal changes in zinc metabolism in individuals with type 2 diabetes mellitus and controversies remain regarding the effect of zinc supplementation in the improvement of oxidative stress in these patients.Faced with the serious challenge of the metabolic disorders related to oxidative stress in diabetes along with the importance of antioxidant nutrients in the control of this disease,new studies may contribute to improve our understanding of the role played by zinc against oxidative stress and its connection with type 2 diabetes mellitus prognosis.This could serve as a prelude to the development of prevention strategies and treatment of disorders associated with this chronic disease. 展开更多
关键词 Diabetes MELLITUS Type 2 OXIDATIVE stress zinc superoxide dismutase Metabolism
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Effect of Jasmonic Acid on Photosynthetic Pigments and Stress Markers in <i>Cajanus cajan</i>(L.) Millsp. Seedlings under Copper Stress 被引量:3
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作者 Sharma Poonam Harpreet Kaur Sirhindi Geetika 《American Journal of Plant Sciences》 2013年第4期817-823,共7页
Jasmonates are class of plant growth regulators act as signal molecule that intercede various components in physiological and metabolic regulation, stress responses and possibly communication through signal transducti... Jasmonates are class of plant growth regulators act as signal molecule that intercede various components in physiological and metabolic regulation, stress responses and possibly communication through signal transduction. Oxidative stress due to heavy metal exposure stimulates synthesis and activity of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues. The aim of this study was to investigate the exogenous effect of JA at seed level which can transduce throughout seedling growth and regulate antioxidant activities such as superoxide dismutase (SOD;EC 1.15.1.1) and guaiacol peroxidase (POD;EC 1.11.1.7) in 12 days old seedlings of pigeon pea (Cajanus cajan (L.) Millsp.) in presence and/or absence of copper. The activity of SOD and POD increased significantly in presence of Cu2+ after seed priming with JA. JA also helps in chlorophyll and carotenoid accumulation and neutralizes the toxic effect of Cu2+ on seedlings. This is the first report of JA effect on photosynthetic pigment accumulation and H2O2 mitigating enzymes i.e. SOD and POD and it could be recommended that seed priming with JA help in ameliorating toxic effect of Cu2+. 展开更多
关键词 Jasmonic Acid copper Sulphate Lipid PEROXIDATION superoxide dismutase GUAIACOL PEROXIDASE Total Chlorophyll Carotenoids
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A Novel Copper(Ⅱ) Complex of Asymmetrically N-Functionalized 1,4,7-Triazacyclononane Ligand: Syntheses, Structure, Electrochemical Property and Biological Activities 被引量:2
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作者 李庆祥 王武伦 孟祥高 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2018年第6期878-886,共9页
A novel copper(Ⅱ) complex derived from 1,4,7-triazacyclononane[CuL]_2(PF_6)_3×MeCN×H_2 O was synthesized and crystallographically characterized {L = 1,4-bis(2-carbamoylethyl)-7-benzimidazole-2-yl-meth... A novel copper(Ⅱ) complex derived from 1,4,7-triazacyclononane[CuL]_2(PF_6)_3×MeCN×H_2 O was synthesized and crystallographically characterized {L = 1,4-bis(2-carbamoylethyl)-7-benzimidazole-2-yl-methyl-1,4,7-triazacyclononane}. It crystallizes in triclinic, space group P1, with a = 13.2425(13), b = 14.0807(15), c = 17.6798(18), α = 86.296(2), β = 72.773(2), γ= 68.905(2)o, V = 2934.5(5)A^3, Z = 2, D_c = 1.611 g/m^3, F(000) = 1456, M_r = 1423.09, m = 0.920 mm^-1. The final R = 0.0671 and wR = 0.1874 for 6501 observed reflections with I 〉 2σ(I). The structural analysis shows that the complex cation([CuL]_2^3+) was formed by two complex cations, namely([CuL^3]^2+) and [CuL_(-H)~3]~+) through a hydrogen bond. In each complex cation, the Cu(Ⅱ) lies in a distorted square pyramidal geometry. The redox behavior was studied by cyclic voltammetry(CV) in aqueous solution which indicates a reversible one electron redox reaction. The result of UV absorption, ethidium bromide(EB) fluorescence spectra indicated that the complex binds to CT-DNA in an intercalative mode. Superoxide dismutase(SOD) activity of the complex was determined by photoreduction of NBT, and the value of IC_(50) is 5.22 μmol·L^-1. 展开更多
关键词 1 4 7-triazacyclononane copper(Ⅱ) COMPLEX crystal structure electrochemical property DNA superoxide dismutase
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Optimal dose of zinc supplementation for preventing aluminum-induced neurotoxicity in rats 被引量:1
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作者 Hao Lu Jianyang Hu +7 位作者 Jing Li Wei Pang Yandan Hu Hongpeng Yang Wenjie Li Chengyu Huang Mingman Zhang Yugang Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第29期2754-2762,共9页
Zinc supplementation can help maintain learning and memory function in rodents. In this study, we hypothesized that zinc supplementation could antagonize the neurotoxicity induced by aluminum in rats. Animals were fed... Zinc supplementation can help maintain learning and memory function in rodents. In this study, we hypothesized that zinc supplementation could antagonize the neurotoxicity induced by aluminum in rats. Animals were fed a diet containing different doses of zinc (50, 100, 200 mg/kg) for 9 weeks, and orally administered aluminum chloride (300 mg/kg daily) from the third week for 7 consecutive weeks. Open-field behavioral test results showed that the number of rearings in the group given the 100 mg/kg zinc supplement was significantly increased compared with the group given the 50 mg/kg zinc supplement. Malondialdehyde content in the cerebrum was significantly decreased, while dopamine and 5-hydroxytryptamine levels were increased in the groups given the diet sup- plemented with 100 and 200 mg/kg zinc, compared with the group given the diet supplemented with 50 mg/kg zinc. The acetylcholinesterase activity in the cerebrum was significantly decreased in the group given the 100 mg/kg zinc supplement. Hematoxylin-eosin staining revealed evident patho- logical damage in the hippocampus of rats in the group given the diet supplemented with 50 mg/kg zinc, but the damage was attenuated in the groups given the diet supplemented with 100 and 200 mg/kg zinc. Our findings suggest that zinc is a potential neuroprotective agent against alumi-num-induced neurotoxicity in rats, and the optimal dosages are 100 and 200 mg/kg. 展开更多
关键词 neural regeneration brain injury ALUMINUM zinc trace elements behavior pathology cerebrummalondialdehyde superoxide dismutase ACETYLCHOLINESTERASE DOPAMINE grants-supported paperneuroregeneration
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Synthesis and Structure of Zinc Complex of N, N-bis ( benzimidazol-2-yl-methyl ) amine
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作者 Xiao Lan LIU Qing Yan CHENG +3 位作者 Xia YANG Zhi Wei MIAO Wei LIU Fang Ming MIAO 《Chinese Chemical Letters》 SCIE CAS CSCD 2001年第6期563-564,共2页
The Zinc complex of N, N-bis (benzimidazol 2-yl-methy])amine has been synthesized and its crystal structure determined by X-ray diffraction method. The structure features of the complex are described.
关键词 zinc compex N N-bis (benzimidazol-2-yl-methyl)amine superoxide dismutase (SOD)
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小麦超氧化物歧化酶基因家族鉴定及盐胁迫下响应锌钾的表达分析 被引量:3
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作者 杨林林 韩敏琦 +1 位作者 高嘉 杨胜敏 《山东农业科学》 北大核心 2023年第8期11-20,共10页
超氧化物歧化酶(SOD)是抗氧化系统的关键酶,在保护植物免受各种生物和非生物胁迫方面发挥着至关重要的作用。然而关于小麦(Triticum aestivum L.)SOD基因家族对盐胁迫的表达模式及响应锌(Zn)和钾(K)的功能知之甚少。本研究以‘西农979... 超氧化物歧化酶(SOD)是抗氧化系统的关键酶,在保护植物免受各种生物和非生物胁迫方面发挥着至关重要的作用。然而关于小麦(Triticum aestivum L.)SOD基因家族对盐胁迫的表达模式及响应锌(Zn)和钾(K)的功能知之甚少。本研究以‘西农979’为试验材料,基于全基因组分析,对小麦SOD家族成员进行鉴定和生物信息学分析,并分析相关基因在盐胁迫下响应Zn、K的表达情况。结果表明,在小麦中共鉴定出10个SOD基因(TaSDs),包括4个Cu/Zn-SOD基因(TaCSD1~TaCSD4)、4个Fe-SOD基因(TaFSD1~TaFSD4)和2个Mn-SOD基因(TaMSD1、TaMSD2)。系统发育分析表明,TaSDs可分为Cu/Zn-SODs、Fe/Mn-SODs两大类,共7个亚组。基因结构、序列基序及启动子分析表明,所有TaSDs都具有1~7个内含子和2~7个外显子,不同TaSDs具有不同的氨基酸序列组成和高度保守的活性位点残基,且每个TaSDs的启动子中存在许多与应激反应和植物激素相关的顺式作用元件。转录组表达谱分析表明,不同TaSDs对盐分表现出不同的表达模式,TaCSD3可能是响应盐胁迫的关键基因;经RT-qPCR分析明确,TaCSD3是响应盐胁迫的靶向SOD基因,同时受Zn、K协同正向调控。本研究结果可为后续小麦耐盐育种改良提供理论依据。 展开更多
关键词 小麦 超氧化物歧化酶 基因组 盐胁迫 锌-钾相互作用
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AECOPD患者血清MnSOD、CuZnSOD变化及其与疾病转归的关系 被引量:3
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作者 曹启凤 刘永琴 杨楠 《国际检验医学杂志》 CAS 2023年第16期1934-1937,1943,共5页
目的分析慢性阻塞性肺疾病急性加重期(AECOPD)患者血清锰超氧化物歧化酶(MnSOD)、铜锌超氧化物歧化酶(CuZnSOD)变化及其与疾病转归的关系。方法将2018年11月至2021年12月该院收治的120例AECOPD患者作为AECOPD组,同期75例慢性阻塞性肺疾... 目的分析慢性阻塞性肺疾病急性加重期(AECOPD)患者血清锰超氧化物歧化酶(MnSOD)、铜锌超氧化物歧化酶(CuZnSOD)变化及其与疾病转归的关系。方法将2018年11月至2021年12月该院收治的120例AECOPD患者作为AECOPD组,同期75例慢性阻塞性肺疾病稳定期患者作为稳定期组,另选取在该院体检的健康者60例作为对照组。所有AECOPD患者出院后随访1年,AECOPD组根据疾病转归情况分为好转组(n=84),恶化组(n=36)。所有纳入对象入院后均检测血清MnSOD、CuZnSOD水平,比较各组血清MnSOD、CuZnSOD水平变化。采用受试者工作特性曲线(ROC)评估血清MnSOD、CuZnSOD对AECOPD患者疾病转归的预测价值。结果AECOPD组血清MnSOD水平明显低于稳定期组、对照组,血清CuZnSOD水平明显高于稳定期组、对照组,差异有统计学意义(P<0.05)。恶化组血清MnSOD水平明显低于好转组,血清CuZnSOD水平明显高于好转组,差异有统计学意义(P<0.05)。ROC曲线分析显示,血清MnSOD预测AECOPD患者疾病转归的曲线下面积(AUC)为0.874,截断值5.58 U/mL,灵敏度、特异度分别为80.56%、78.57%,血清CuZnSOD预测AECOPD患者疾病转归的AUC为0.845,截断值66.62 U/mL,灵敏度、特异度分别为83.33%、70.32%。结论AECOPD患者血清MnSOD水平降低,CuZnSOD水平升高,二者与患者疾病转归密切相关,在诊断AECOPD患者疾病转归中具有良好的效能。 展开更多
关键词 慢性阻塞性肺疾病急性加重期 锰超氧化物歧化酶 铜锌超氧化物歧化酶 疾病转归
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编码棉花胞质铜锌超氧物歧化酶基因的克隆与表达分析 被引量:19
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作者 胡根海 喻树迅 +1 位作者 范术丽 宋美珍 《中国农业科学》 CAS CSCD 北大核心 2007年第8期1602-1609,共8页
【目的】克隆编码棉花胞质铜锌超氧化物岐化酶基因并分析其表达特性。【方法】采用RACE技术克隆基因,Northern blotting检测基因的表达谱;采用氮蓝四唑(NBT)光下还原法测定不同生育期的酶活性。【结果】获得了棉花胞质铜锌超氧化物岐化... 【目的】克隆编码棉花胞质铜锌超氧化物岐化酶基因并分析其表达特性。【方法】采用RACE技术克隆基因,Northern blotting检测基因的表达谱;采用氮蓝四唑(NBT)光下还原法测定不同生育期的酶活性。【结果】获得了棉花胞质铜锌超氧化物岐化酶基因cDNA全长序列(GenBank注册号:DQ445093);该基因cDNA全长共682bp,开放阅读框456bp,编码152个氨基酸。分子结构预测结果:酶蛋白理论分子量约为15.03kD,理论等电点为6.09,与其它植物的蛋白质氨基酸序列同源性在82%~87%之间。Southern blotting显示不同棉种该基因的拷贝数基本一致,均属于低拷贝基因。Northern blotting显示该基因在不同的组织、不同的生育期表达量不同;酶活性测定显示盛花期最高。【结论】棉花胞质铜锌超氧化物岐化酶基因在陆地棉中属于低拷贝数基因;在整个生育期中mRNA的含量呈规律性动态变化,前期较低,后期较高,在盛花期达到顶峰;变化曲线与不同时期的酶活性变化一致;不同器官的基因表达检测结果显示:基因在根中表达量最高,叶片次之,花中的表达最低。 展开更多
关键词 棉花 铜锌超氧物歧化酶 基因 克隆
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锌、锰、铁和铜离子对水稻幼苗生长及SOD活性的影响 被引量:22
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作者 邵邻相 黄伯钟 丁淑静 《种子》 CSCD 北大核心 2001年第6期16-17,19,共3页
用一定浓度的 Zn2 + 、Mn2 + 、Fe2 + 和 Cu2 + 溶液培养水稻幼苗 ,对水稻幼苗生长及生理活性物质的影响进行研究。结果表明 :用 Mn SO4 溶液培养的幼苗新生叶尖全部枯黄 ,叶片相对较短 ,根系生长受到抑制。用 Zn SO4 、Cu SO4 溶液培... 用一定浓度的 Zn2 + 、Mn2 + 、Fe2 + 和 Cu2 + 溶液培养水稻幼苗 ,对水稻幼苗生长及生理活性物质的影响进行研究。结果表明 :用 Mn SO4 溶液培养的幼苗新生叶尖全部枯黄 ,叶片相对较短 ,根系生长受到抑制。用 Zn SO4 、Cu SO4 溶液培养的幼苗 ,叶呈黄绿色 ,苗株矮小 ,根系衰退。用 Fe SO4 溶液培养的幼苗 ,叶呈深绿色 ,苗株长势旺盛 ,根系长势良好。Mn2 +使水稻超氧化物歧化酶 (SOD)活性明显减弱 ,Cu2 +、Fe2 +使水稻 SOD活性明显增强 ,Zn2 +对水稻 SOD活性无明显影响。不同金属离子浓度处理对水稻酯酶活性无明显影响。 展开更多
关键词 水稻 超氧化物歧化酶 酶活性 幼苗生长
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补阳还五汤对球囊扩张主动脉后再狭窄其内皮PDGFR和SOD-1基因表达的探讨 被引量:67
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作者 谢全锦 侯灿 +1 位作者 吴伟康 何勤 《中国中西医结合杂志》 CAS CSCD 北大核心 1997年第10期611-613,共3页
目的:探讨补阳还五汤防治经皮腔内冠脉成形术(PTCA)后再狭窄的消长机制。方法:仿PTCA术后再狭窄的新西兰兔模型28只,以原位引物标记法测动脉内皮钢-锌超氧化物歧化酶(SOD-1)及血小板行化生长因子受体(PDGF... 目的:探讨补阳还五汤防治经皮腔内冠脉成形术(PTCA)后再狭窄的消长机制。方法:仿PTCA术后再狭窄的新西兰兔模型28只,以原位引物标记法测动脉内皮钢-锌超氧化物歧化酶(SOD-1)及血小板行化生长因子受体(PDGFR)的基因表达,分析再狭窄消长机理。结果:主动脉球囊扩张术后7天,手术部位动脉内皮的SOD-1基因表达增强,与对照组有显著差异(P<0.05),而PDGFRmRNA的表达则减弱,与对照组也有显著性差异(P<0.05)。结论:补阳还五汤增强动脉内皮SOD-1的基因表达,改善缺血再灌注的供氧。同样情况下,补阳还五汤抑制PDGFRmRNA表达,以控制细胞的病理性增殖。 展开更多
关键词 补阳还五汤 生长因子 冠状动脉狭窄 血管成形术
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可见吸收光谱法研究铜锌超氧歧化酶活性中心金属离子与氯化钴的相互作用 被引量:9
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作者 郑学仿 胡皆汉 +1 位作者 许永廷 程国宝 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 1997年第5期35-39,共5页
应用可见吸收光谱首次研究了铜锌超氧歧化酶(Cu2Zn2SOD)活性中心金属离子在一定缓冲溶液中与无机氯化钴的直接相互作用。讨论了加入不同比例量的氯化钴、不同的pH值、不同酸盐及作用的平衡时间对这种相互作用的影响。结果... 应用可见吸收光谱首次研究了铜锌超氧歧化酶(Cu2Zn2SOD)活性中心金属离子在一定缓冲溶液中与无机氯化钴的直接相互作用。讨论了加入不同比例量的氯化钴、不同的pH值、不同酸盐及作用的平衡时间对这种相互作用的影响。结果发现,CoCl2中的Co(Ⅱ)与Cu2Zn2SOD中的Zn(Ⅱ)有交换作用,形成部分的Cu2中的Co2SOD,且上述四种因素对此均有不同程度的影响。本研究开创了金属酶活性中心金属离子与外加无机金属化合物的直接相互作用的研究。 展开更多
关键词 铜锌超氧歧化酶 氯化钴 可见吸收光谱 SOD
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鸡红细胞Cu,Zn-SOD的纯化及部分性质研究 被引量:9
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作者 张兰杰 候冬岩 +1 位作者 辛广 袁勤生 《食品科学》 EI CAS CSCD 北大核心 2008年第2期266-270,共5页
目的:从鸡红细胞分离铜锌超氧化物歧化酶(Cu,Zn-SOD),并对其部分性质进行研究。方法:用氯仿-乙醇除杂蛋白、丙酮沉淀、热变性、DEAE-32柱色谱等方法,对鸡红细胞Cu,Zn-SOD进行分离纯化。结果:得到Cu,Zn-SOD干粉5.9mg,比活10196.27U/mg蛋... 目的:从鸡红细胞分离铜锌超氧化物歧化酶(Cu,Zn-SOD),并对其部分性质进行研究。方法:用氯仿-乙醇除杂蛋白、丙酮沉淀、热变性、DEAE-32柱色谱等方法,对鸡红细胞Cu,Zn-SOD进行分离纯化。结果:得到Cu,Zn-SOD干粉5.9mg,比活10196.27U/mg蛋白,活力回收70.43%,纯化倍数为125.82。理化性质分析表明:该酶的最大紫外吸收波长264nm,相对分子量约为31000D,相对亚基分子量约为16500D,pH在6~9范围内稳定性很好,在40~60℃内保温30min酶活基本不变,2mmol/LSDS对此酶没有明显的抑制,该酶对H2O2和KCN敏感。结论:鸡红细胞含有丰富的Cu,Zn-SOD。 展开更多
关键词 鸡红细胞 铜锌超氧化物歧化酶 纯化 性质
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桃果实铜锌超氧化物歧化酶基因PpCuZnSOD的克隆与分析 被引量:10
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作者 吴军帅 李培环 +2 位作者 李鼎立 董晓颖 段艳欣 《核农学报》 CAS CSCD 北大核心 2013年第4期408-417,共10页
从已构建的硬肉桃(Prunus persica L.)双久红果实SSH文库中获得4条铜锌超氧化物歧化酶(CuZnSOD)基因的ESTs,通过blast比对和电子克隆后进行了序列拼接和经RT-PCR方法,获得桃果实铜锌超氧化物歧化酶基因PpCuZnSOD全长cDNA序列(GenBank登... 从已构建的硬肉桃(Prunus persica L.)双久红果实SSH文库中获得4条铜锌超氧化物歧化酶(CuZnSOD)基因的ESTs,通过blast比对和电子克隆后进行了序列拼接和经RT-PCR方法,获得桃果实铜锌超氧化物歧化酶基因PpCuZnSOD全长cDNA序列(GenBank登录号:JX217743)。该基因全长665bp,包含一个459bp的ORF(120bp-578bp),编码152个氨基酸,推测蛋白分子量为15.38kDa,理论等电点为5.60。该基因具有典型的高度保守的Cu2+和Zn2+活化中心及特征信号,其氨基酸序列与其他植物的CuZnSOD具有很高的相似性。生物信息学分析结果表明,该蛋白定位于细胞质中,是非跨膜的非分泌性亲水蛋白。二级结构分析结果表明,该蛋白主要由无规则卷曲和延伸链等蛋白质二级结构元件构成,三维结构预测结果显示,PpCuZnSOD有3个转角环和8个折叠构成桶状的活性中心。系统进化分析结果表明PpCuZnSOD与小金海棠(Malus xiaojinensis)sod2同源关系最近,而与荔枝(Lichichinensis)、龙眼(Dimocarpus longan)和拟南芥(Arabidopsis trifoliate)等亲缘关系较远。QPCR结果表明,PpCuZnSOD在幼果中表达量最高,雌蕊和雄蕊次之,花瓣和叶片中表达量最少;果实成熟前后,PpCuZnSOD在硬肉芽变桃双久红果实中的表达量显著高于软肉桃川中岛白桃中的表达量,表明该基因可能在维持果实质地和硬度方面有一定作用。本研究结果为进一步研究该基因在桃果实成熟软化中的作用奠定了基础。 展开更多
关键词 PpCuZnSOD基因 克隆 生物信息学 基因表达
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不同铜钼水平对小尾寒羊血清铜蓝蛋白和血浆过氧化物歧化酶活性的影响 被引量:14
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作者 吕爱军 杨在宾 +3 位作者 杨维仁 姜淑贞 张桂国 展振锋 《动物营养学报》 CAS CSCD 北大核心 2005年第1期61-64,共4页
本试验选用9只体况良好、体重相近、1岁内小尾寒羊半同胞羯羊作为试验动物,研究了日粮中添加不同铜、钼水平对小尾寒羊血清CPL和血浆SOD活性的影响。试验分两期进行,每期随机分为3组,每组3只。第一期试验不添加钼(基础日粮钼水平:0.31mg... 本试验选用9只体况良好、体重相近、1岁内小尾寒羊半同胞羯羊作为试验动物,研究了日粮中添加不同铜、钼水平对小尾寒羊血清CPL和血浆SOD活性的影响。试验分两期进行,每期随机分为3组,每组3只。第一期试验不添加钼(基础日粮钼水平:0.31mg/kg),第二期在基础日粮的基础上添加2.5mg/kg钼。每期铜的添加量分别为5、10、15mg/kg。结果表明:在低钼日粮条件下,提高铜水平不影响血清CPL的活性(P>0.05);日粮中添加2.5mg/kg钼时,铜水平的提高可显著增加血清CPL的活性(P<0.05)。当铜水平一定时,提高日粮钼水平可降低血清CPL和血浆SOD的活性(P<0.05);而当钼水平一定时,提高铜水平并不能增加血浆SOD的活性(P>0.05)。日粮钼水平为0.31mg/kg时,添加5mg/kg铜可满足机体需要;日粮钼水平为2.81mg/kg时,添加15mg/kg铜较适宜。羊在缺铜时,与血浆SOD的活性比较,血清CPL更宜作为评价铜营养缺乏的指标;铜满足机体需要后,血清CPL和血浆SOD均对铜和钼的变化不敏感。 展开更多
关键词 血清铜蓝蛋白 血浆过氧化物歧化酶 绵羊
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Phen-铜(Ⅱ)-氨基酸配合物的合成、表征及其SOD活性 被引量:15
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作者 廖升荣 乐学义 +4 位作者 林庆斌 卢其明 刘小平 熊亚红 冯小龙 《无机化学学报》 SCIE CAS CSCD 北大核心 2006年第2期201-206,共6页
合成了3个新的SOD模拟配合物:[Cu(Phen)(L-Gln)(H2O)]Cl·2H2O(1)、[Cu(Phen)(L-Ala)(H2O)]Cl·4H2O(2)、[Cu(Phen)(L-Thr)(H2O)]Cl·2H2O(3)[Phen(1,10-邻菲咯啉)、L-Gln(谷氨酰胺)、L-Ala(丙氨酸)、L-Thr(苏氨酸)]。用元... 合成了3个新的SOD模拟配合物:[Cu(Phen)(L-Gln)(H2O)]Cl·2H2O(1)、[Cu(Phen)(L-Ala)(H2O)]Cl·4H2O(2)、[Cu(Phen)(L-Thr)(H2O)]Cl·2H2O(3)[Phen(1,10-邻菲咯啉)、L-Gln(谷氨酰胺)、L-Ala(丙氨酸)、L-Thr(苏氨酸)]。用元素分析、摩尔电导、红外光谱、紫外-可见光谱对配合物进行了表征。用X-射线衍射对配合物[Cu(Phen)(L-Gln)(H2O)]Cl·2H2O的晶体结构进行了测定。用氯化硝基四氮唑蓝(NBT)光还原法对这3个配合物催化歧化超氧阴离子自由基(O2-·)的能力进行了测定。结果表明:这些配合物具有较高的SOD活性,催化速率常数KQ分别为1.58×107mol-1·L·s-1、5.65×107mol-1·L·s-1、0.83×107mol-1·L·s-1。 展开更多
关键词 超氧化物歧化酶 模拟物 SOD活性
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水杨酸与烟草铜锌超氧化物歧化酶的相互作用 被引量:4
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作者 盛良全 刘少民 +3 位作者 贾莉莉 徐小龙 解永树 刘清亮 《分析化学》 SCIE EI CAS CSCD 北大核心 2003年第3期283-286,共4页
利用邻苯三酚自氧化法监测了磷酸盐缓冲体系中水杨酸 (SA)对超氧化物歧化酶 (SOD)活力的影响。通过荧光光谱法研究了水杨酸与超氧化物歧化酶的相互作用机制 ,求得两者结合的形成常数和配位数 ,使用F rster非辐射能量转移技术求出了两者... 利用邻苯三酚自氧化法监测了磷酸盐缓冲体系中水杨酸 (SA)对超氧化物歧化酶 (SOD)活力的影响。通过荧光光谱法研究了水杨酸与超氧化物歧化酶的相互作用机制 ,求得两者结合的形成常数和配位数 ,使用F rster非辐射能量转移技术求出了两者的结合位置距色氨酸残基间的距离为 2 .60nm。 展开更多
关键词 水杨酸 铜锌超氧化物歧化酶 酶活力 荧光光谱 烟草 相互作用 邻苯三酚自氧化法
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