Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Metho...Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Methods:Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n =15)and HFD group (n =45).After 8 weeks of feeding,the HFD group was further divided into three groups:a group provided with a combination of HLJJ and HFD for 4 weeks,a group provided with a combination of berberine and HFD for 4 weeks,and a group supplied only with HFD for 4 consecutive weeks.Epididymal fat weight,serum high-density lipoproteincholesterohC (HDL-C),lowdensity lipoproteincholesterol-C (LDL-C) and irisin levels were measured.In addition,the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform.Results:MS was successfully induced in the rats with HFD for 8 weeks.After 12 weeks,serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P <.05).The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level.Furthermore,at the genus level,Akkermansia muciniphila (A.muciniphila),Bacteroides,and Ruminococcus were markedly increased,whereas Candidatus arthromitus,Prevotella,Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group,compared with the HFD group.The abundance of A.muciniphila,Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group,whereas,the abundance of Oscillospira was lower in the berberine group,compared with the HLJJ group.Conclusion:HLJJ and berberine changed the gut microbiota in rats with MS.The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.展开更多
葛根芩连汤治疗2型糖尿病(diabetes mellitus type 2,T2DM),可降低血糖水平、改善糖脂代谢及胰岛功能等。实验研究也表明,葛根芩连汤可通过改善胰岛素抵抗、调节糖脂代谢、调节肠道菌群、抑制炎症因子表达等控制血糖水平,缓解T2DM病情...葛根芩连汤治疗2型糖尿病(diabetes mellitus type 2,T2DM),可降低血糖水平、改善糖脂代谢及胰岛功能等。实验研究也表明,葛根芩连汤可通过改善胰岛素抵抗、调节糖脂代谢、调节肠道菌群、抑制炎症因子表达等控制血糖水平,缓解T2DM病情。目前的研究仍存在不足之处:葛根芩连汤在治疗T2DM的应用中,缺乏统一规范的疗效判定标准及大量的临床样本,其具体的分子生物学机制尚不明确。此外,葛根芩连汤原方中“两”的剂量历来没有统一的规范,以往的临床应用与研究中,各医家对其具体剂量的使用均有不同,主要从临床经验入手来确定其具体用量,缺乏严谨性,不便于临床规范应用。今后,应结合现代分子生物学技术探讨葛根芩连汤治疗T2DM的具体机制,并建立统一规范的疗效判定标准和剂量使用标准,充分发挥中医药治疗T2DM的优势,为提高疗效及扩大临床应用补充有利依据。展开更多
目的:采用网络药理学和分子对接的方法研究黄连解毒汤治疗乙型脑炎(Japanese encephalitis,JE)的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platfo...目的:采用网络药理学和分子对接的方法研究黄连解毒汤治疗乙型脑炎(Japanese encephalitis,JE)的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)获得黄连解毒汤4味中药的化学成分及相关靶点。在GeneCards数据库、DisGeNET数据库和在线人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)中检索JE疾病相关靶点。取活性成分相关靶点与JE相关靶点的交集为黄连解毒汤治疗JE的潜在作用靶点。将潜在靶点导入String数据库进行蛋白质互作(protein-protein interaction,PPI)分析,并采用Cytoscape v 3.8.2软件进行可视化。使用DAVID数据库对潜在作用靶点进行基因本体(gene ontology,GO)功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)信号通路富集分析。利用Cytoscape v 3.8.2软件构建“活性成分-潜在靶点-信号通路”网络,拓扑分析筛选核心成分和靶点。采用Autodock Vina软件进行分子对接,将结果导入Pymol软件做可视化分析。结果:通过TCMSP数据库筛选出黄连解毒汤活性成分63个,相关靶点202个。通过GeneCards数据库、DisGeNET数据库及OMIM数据库得到JE相关靶点1065个,黄连解毒汤治疗JE的潜在作用靶点85个。PPI网络分析得到10个核心靶点。GO功能富集分析得到600个条目,其中生物过程条目468个,分子功能条目83个,细胞组成条目49个。KEGG通路富集分析得到142条信号通路。“活性成分-潜在靶点-信号通路”网络分析筛选得到槲皮素、β-谷甾醇、汉黄芩素等10个关键活性成分及丝裂原激活蛋白激酶1(mitogen activation protein kinase 1,MAPK1)、JUN等10个重要靶点。分子对接结果表明,关键活性成分与核心靶点结合良好。结论:黄连解毒汤通过多个成分、多个靶点、多条通路发挥治疗JE的作用。展开更多
文摘Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Methods:Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n =15)and HFD group (n =45).After 8 weeks of feeding,the HFD group was further divided into three groups:a group provided with a combination of HLJJ and HFD for 4 weeks,a group provided with a combination of berberine and HFD for 4 weeks,and a group supplied only with HFD for 4 consecutive weeks.Epididymal fat weight,serum high-density lipoproteincholesterohC (HDL-C),lowdensity lipoproteincholesterol-C (LDL-C) and irisin levels were measured.In addition,the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform.Results:MS was successfully induced in the rats with HFD for 8 weeks.After 12 weeks,serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P <.05).The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level.Furthermore,at the genus level,Akkermansia muciniphila (A.muciniphila),Bacteroides,and Ruminococcus were markedly increased,whereas Candidatus arthromitus,Prevotella,Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group,compared with the HFD group.The abundance of A.muciniphila,Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group,whereas,the abundance of Oscillospira was lower in the berberine group,compared with the HLJJ group.Conclusion:HLJJ and berberine changed the gut microbiota in rats with MS.The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.
文摘葛根芩连汤治疗2型糖尿病(diabetes mellitus type 2,T2DM),可降低血糖水平、改善糖脂代谢及胰岛功能等。实验研究也表明,葛根芩连汤可通过改善胰岛素抵抗、调节糖脂代谢、调节肠道菌群、抑制炎症因子表达等控制血糖水平,缓解T2DM病情。目前的研究仍存在不足之处:葛根芩连汤在治疗T2DM的应用中,缺乏统一规范的疗效判定标准及大量的临床样本,其具体的分子生物学机制尚不明确。此外,葛根芩连汤原方中“两”的剂量历来没有统一的规范,以往的临床应用与研究中,各医家对其具体剂量的使用均有不同,主要从临床经验入手来确定其具体用量,缺乏严谨性,不便于临床规范应用。今后,应结合现代分子生物学技术探讨葛根芩连汤治疗T2DM的具体机制,并建立统一规范的疗效判定标准和剂量使用标准,充分发挥中医药治疗T2DM的优势,为提高疗效及扩大临床应用补充有利依据。
文摘目的:采用网络药理学和分子对接的方法研究黄连解毒汤治疗乙型脑炎(Japanese encephalitis,JE)的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)获得黄连解毒汤4味中药的化学成分及相关靶点。在GeneCards数据库、DisGeNET数据库和在线人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)中检索JE疾病相关靶点。取活性成分相关靶点与JE相关靶点的交集为黄连解毒汤治疗JE的潜在作用靶点。将潜在靶点导入String数据库进行蛋白质互作(protein-protein interaction,PPI)分析,并采用Cytoscape v 3.8.2软件进行可视化。使用DAVID数据库对潜在作用靶点进行基因本体(gene ontology,GO)功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)信号通路富集分析。利用Cytoscape v 3.8.2软件构建“活性成分-潜在靶点-信号通路”网络,拓扑分析筛选核心成分和靶点。采用Autodock Vina软件进行分子对接,将结果导入Pymol软件做可视化分析。结果:通过TCMSP数据库筛选出黄连解毒汤活性成分63个,相关靶点202个。通过GeneCards数据库、DisGeNET数据库及OMIM数据库得到JE相关靶点1065个,黄连解毒汤治疗JE的潜在作用靶点85个。PPI网络分析得到10个核心靶点。GO功能富集分析得到600个条目,其中生物过程条目468个,分子功能条目83个,细胞组成条目49个。KEGG通路富集分析得到142条信号通路。“活性成分-潜在靶点-信号通路”网络分析筛选得到槲皮素、β-谷甾醇、汉黄芩素等10个关键活性成分及丝裂原激活蛋白激酶1(mitogen activation protein kinase 1,MAPK1)、JUN等10个重要靶点。分子对接结果表明,关键活性成分与核心靶点结合良好。结论:黄连解毒汤通过多个成分、多个靶点、多条通路发挥治疗JE的作用。