A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Thei...A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Their molar ratio is 1 00∶1 58∶7 89∶0 19. The crude polysaccharide was fractionated with ethanol to get three fractions, which were CM Ⅰ, CM Ⅱ and CM Ⅲ. CM Ⅱ was further purified to get one homogeneous fraction named CMB. The molecular weight was estimated to be 60000 by means of gel filtration. CMB is a greyish white powder. The GC analysis indicated that it was composed of Gal, Man, Glc and the molar ratio is 1 00∶1 38∶5 10. Partial hydrolysis with an acid, periodate oxidation, Smith degradation, IR, GC and methylation analyses, GC/MS analyses were used for the structure analyses of CMB. The results reveal that CMB is of a lower branched structure. The main chain is composed of (1→6) linked Man, (1→6) linked Glc, (1→4) linked Glc and (1→4) linked Gal. Some of (1→4) linked Glc and all (1→4) linked Gal residues are substituted at 3 O and 6 O respectively. (1→4) Linked Glc residues without branch probably exist with other monosaccharides alternately. On an average, there is one branch every ten hexose residues. The side chain is composed of major (1→4) linked Glc and minor (1→6) linked Glc. The nonreduced end is composed of Gal and Glc. The main glycosidic link of CMB is α configuration.展开更多
目的:探讨蓝光诱导对蛹虫草Cordyceps militaris L.菌丝体和子实体中胞内多糖的单糖组成以及虫草素和虫草多糖含量的影响。方法:以蛹虫草为材料,使用气相色谱检测蓝光照射144 h后菌丝体及子实体中多糖的单糖组成;并在蓝光照射下的不同...目的:探讨蓝光诱导对蛹虫草Cordyceps militaris L.菌丝体和子实体中胞内多糖的单糖组成以及虫草素和虫草多糖含量的影响。方法:以蛹虫草为材料,使用气相色谱检测蓝光照射144 h后菌丝体及子实体中多糖的单糖组成;并在蓝光照射下的不同时间段分别取样,以检测菌丝体及子实体中虫草素和虫草多糖的含量。同时观察蛹虫草菌丝体和子实体在蓝光照射诱导过程中的生长情况。结果:蛹虫草菌丝体多糖是一种含有4种单糖的杂多糖;子实体多糖是一种含有5种单糖的杂多糖。在菌丝体多糖的检测试验中,无论是蓝光照射还是黑暗培养,两组的多糖含量变化规律几乎相似,都是在一定的时间段开始发生变化。在子实体多糖的检测中,无论是蓝光照射还是白光对照,两组多糖含量的变化规律也基本相同。在菌丝体以及子实体虫草素的检测中,虫草素含量在两组实验中均呈现出明显的上升趋势。结论:蓝光诱导对蛹虫草胞内多糖中单糖的组成种类和数量有一定的影响。而且蓝光诱导对蛹虫草中虫草素和虫草多糖含量的提高有一定的促进作用。蓝光有利于蛹虫草的形态建成,促进子实体的分化和生长发育。该项研究首次报道了蓝光诱导对蛹虫草多糖中单糖组成的种类和数量的影响,为进一步研究蓝光诱导对蛹虫草活性物质的代谢奠定基础。展开更多
文摘A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Their molar ratio is 1 00∶1 58∶7 89∶0 19. The crude polysaccharide was fractionated with ethanol to get three fractions, which were CM Ⅰ, CM Ⅱ and CM Ⅲ. CM Ⅱ was further purified to get one homogeneous fraction named CMB. The molecular weight was estimated to be 60000 by means of gel filtration. CMB is a greyish white powder. The GC analysis indicated that it was composed of Gal, Man, Glc and the molar ratio is 1 00∶1 38∶5 10. Partial hydrolysis with an acid, periodate oxidation, Smith degradation, IR, GC and methylation analyses, GC/MS analyses were used for the structure analyses of CMB. The results reveal that CMB is of a lower branched structure. The main chain is composed of (1→6) linked Man, (1→6) linked Glc, (1→4) linked Glc and (1→4) linked Gal. Some of (1→4) linked Glc and all (1→4) linked Gal residues are substituted at 3 O and 6 O respectively. (1→4) Linked Glc residues without branch probably exist with other monosaccharides alternately. On an average, there is one branch every ten hexose residues. The side chain is composed of major (1→4) linked Glc and minor (1→6) linked Glc. The nonreduced end is composed of Gal and Glc. The main glycosidic link of CMB is α configuration.
文摘目的:探讨蓝光诱导对蛹虫草Cordyceps militaris L.菌丝体和子实体中胞内多糖的单糖组成以及虫草素和虫草多糖含量的影响。方法:以蛹虫草为材料,使用气相色谱检测蓝光照射144 h后菌丝体及子实体中多糖的单糖组成;并在蓝光照射下的不同时间段分别取样,以检测菌丝体及子实体中虫草素和虫草多糖的含量。同时观察蛹虫草菌丝体和子实体在蓝光照射诱导过程中的生长情况。结果:蛹虫草菌丝体多糖是一种含有4种单糖的杂多糖;子实体多糖是一种含有5种单糖的杂多糖。在菌丝体多糖的检测试验中,无论是蓝光照射还是黑暗培养,两组的多糖含量变化规律几乎相似,都是在一定的时间段开始发生变化。在子实体多糖的检测中,无论是蓝光照射还是白光对照,两组多糖含量的变化规律也基本相同。在菌丝体以及子实体虫草素的检测中,虫草素含量在两组实验中均呈现出明显的上升趋势。结论:蓝光诱导对蛹虫草胞内多糖中单糖的组成种类和数量有一定的影响。而且蓝光诱导对蛹虫草中虫草素和虫草多糖含量的提高有一定的促进作用。蓝光有利于蛹虫草的形态建成,促进子实体的分化和生长发育。该项研究首次报道了蓝光诱导对蛹虫草多糖中单糖组成的种类和数量的影响,为进一步研究蓝光诱导对蛹虫草活性物质的代谢奠定基础。
