A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Thei...A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Their molar ratio is 1 00∶1 58∶7 89∶0 19. The crude polysaccharide was fractionated with ethanol to get three fractions, which were CM Ⅰ, CM Ⅱ and CM Ⅲ. CM Ⅱ was further purified to get one homogeneous fraction named CMB. The molecular weight was estimated to be 60000 by means of gel filtration. CMB is a greyish white powder. The GC analysis indicated that it was composed of Gal, Man, Glc and the molar ratio is 1 00∶1 38∶5 10. Partial hydrolysis with an acid, periodate oxidation, Smith degradation, IR, GC and methylation analyses, GC/MS analyses were used for the structure analyses of CMB. The results reveal that CMB is of a lower branched structure. The main chain is composed of (1→6) linked Man, (1→6) linked Glc, (1→4) linked Glc and (1→4) linked Gal. Some of (1→4) linked Glc and all (1→4) linked Gal residues are substituted at 3 O and 6 O respectively. (1→4) Linked Glc residues without branch probably exist with other monosaccharides alternately. On an average, there is one branch every ten hexose residues. The side chain is composed of major (1→4) linked Glc and minor (1→6) linked Glc. The nonreduced end is composed of Gal and Glc. The main glycosidic link of CMB is α configuration.展开更多
[目的]采用正交法优化蛹虫草(Cordyceps militaris L.Link)子实体多糖的提取工艺。[方法]采用优化煎煮法、水热回流提取法和碱法提取蛹虫草子实体多糖的工艺。[结果]煎煮法提取蛹虫草子实体多糖的最优条件为:加入40倍体积的水,提取3次,...[目的]采用正交法优化蛹虫草(Cordyceps militaris L.Link)子实体多糖的提取工艺。[方法]采用优化煎煮法、水热回流提取法和碱法提取蛹虫草子实体多糖的工艺。[结果]煎煮法提取蛹虫草子实体多糖的最优条件为:加入40倍体积的水,提取3次,每次3.0h,各因素影响得率的主次顺序为:料液比>煎煮时间>煎煮次数。水热回流法提取蛹虫草子实体多糖的最优条件为:加入20倍体积的水,80℃下提取2次,每次1.0h,各因素影响得率的主次顺序为:提取次数>提取时间>提取温度>料液比。碱法提取蛹虫草子实体多糖的最优条件为:加入8倍体积的0.7mol/LNaOH溶液,提取3次,每次0.5h,各因素影响多糖得率的主次顺序为:浸提次数>NaOH浓度>料液比>浸提时间。[结论]该研究找出了煎煮法、水热回流提取法和碱法提取蛹虫草子实体多糖的工艺,可为下一步研究及工业生产提供参考资料。展开更多
文摘A water soluble crude polysaccharide has been isolated from the fruiting body of cultivated Cordyceps militarvs(L.) Link. The GC analysis indicated that it contained the monosaccharides of Gal, Man, Glc and GlcA. Their molar ratio is 1 00∶1 58∶7 89∶0 19. The crude polysaccharide was fractionated with ethanol to get three fractions, which were CM Ⅰ, CM Ⅱ and CM Ⅲ. CM Ⅱ was further purified to get one homogeneous fraction named CMB. The molecular weight was estimated to be 60000 by means of gel filtration. CMB is a greyish white powder. The GC analysis indicated that it was composed of Gal, Man, Glc and the molar ratio is 1 00∶1 38∶5 10. Partial hydrolysis with an acid, periodate oxidation, Smith degradation, IR, GC and methylation analyses, GC/MS analyses were used for the structure analyses of CMB. The results reveal that CMB is of a lower branched structure. The main chain is composed of (1→6) linked Man, (1→6) linked Glc, (1→4) linked Glc and (1→4) linked Gal. Some of (1→4) linked Glc and all (1→4) linked Gal residues are substituted at 3 O and 6 O respectively. (1→4) Linked Glc residues without branch probably exist with other monosaccharides alternately. On an average, there is one branch every ten hexose residues. The side chain is composed of major (1→4) linked Glc and minor (1→6) linked Glc. The nonreduced end is composed of Gal and Glc. The main glycosidic link of CMB is α configuration.
文摘[目的]采用正交法优化蛹虫草(Cordyceps militaris L.Link)子实体多糖的提取工艺。[方法]采用优化煎煮法、水热回流提取法和碱法提取蛹虫草子实体多糖的工艺。[结果]煎煮法提取蛹虫草子实体多糖的最优条件为:加入40倍体积的水,提取3次,每次3.0h,各因素影响得率的主次顺序为:料液比>煎煮时间>煎煮次数。水热回流法提取蛹虫草子实体多糖的最优条件为:加入20倍体积的水,80℃下提取2次,每次1.0h,各因素影响得率的主次顺序为:提取次数>提取时间>提取温度>料液比。碱法提取蛹虫草子实体多糖的最优条件为:加入8倍体积的0.7mol/LNaOH溶液,提取3次,每次0.5h,各因素影响多糖得率的主次顺序为:浸提次数>NaOH浓度>料液比>浸提时间。[结论]该研究找出了煎煮法、水热回流提取法和碱法提取蛹虫草子实体多糖的工艺,可为下一步研究及工业生产提供参考资料。
