The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes sho...The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting;small white embryos appear on the surface of calluses.展开更多
Cork oak in Maamora forest is experiencing the dieback phenomenon. The evaluation of the latter in this forest has gained the importance over time and with the solicitation of managers to objectify its phytosanitary s...Cork oak in Maamora forest is experiencing the dieback phenomenon. The evaluation of the latter in this forest has gained the importance over time and with the solicitation of managers to objectify its phytosanitary situation. Aiming at prioritizing management actions, remote sensing seems to be an effective tool to inquire about stands’ health conditions and their evolution. To this end, this study aims at mapping and validating health status of cork oak stands in Maamora. Sentinel 2 images in 2015 and 2020 were processed to calculate the differential normalized difference water index (NDWI), revealing vegetation moisture variation caused by drought. A statistical method based on thresholds was used to map cork oak dieback stands, those with no changes and those recovered. Results have shown that 54.63% of cork oak in Maamora forest have not changed in terms of phytosanitary situation between 2015 and 2020, 31.10% of oak stands are afflicted by a slight decline and 12.97% by a severe decline. Areas with slight or strong recovery remain minimal and represent 1.04% and 0.25% respectively. Ground data indicated that the map generated displayed a good distinction between stands severely and slightly declined with a global accuracy of 66.66%. Therefore, further research elaborating an advanced vegetation index reflecting the various factors of dieback would be of much importance.展开更多
In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic em...In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N30K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.展开更多
The mineralization of the organic matter is a very important phenomenon which leads to the release of nutriments used by plants. The rate of transformation of the organic matter depends on several factors and paramete...The mineralization of the organic matter is a very important phenomenon which leads to the release of nutriments used by plants. The rate of transformation of the organic matter depends on several factors and parameters such as climatic factors and biological and physicochemical properties of the soil and the litter. In this study, we investigate the effect of the addition of litter of various species as well as the effect of soil moisture on mineralization of organic matter and on mineral nitrogen release in three soils sampled in three cork oak stands during a period of 41 days under the same laboratory conditions. Carbon mineralization was determined using CO2 respiration method, whereas the mineral nitrogen content was measured at the last day of incubation both in soil samples with added plant leaf material, and in control soil samples without addition under two treatments of moisture (40% and 80% WHC—water holding capacity). Our results show that the addition of leaf litter causes an increase in the microbial activity. Soils without addition were significantly different from the plant leaf added soils in respect to carbon mineralization at the end of the incubation period. Moreover, it is noted that the mineralization of carbon is more marked with moisture at 80% than that of 40%, contrary to that of the nitrogen, which is not influenced by the variation of moisture.展开更多
Maamora is considered the most important cork-oak forest in the world with regard to surface. Therefore, anthropic pressure, including cork harvesting, grazing and soft acorn picking up by local communities, has harmf...Maamora is considered the most important cork-oak forest in the world with regard to surface. Therefore, anthropic pressure, including cork harvesting, grazing and soft acorn picking up by local communities, has harmful consequences on forest regeneration and the forest become older exceeding harvesting age. Thus, its sustainability depends on the managers’ ability to succeed cork oak plantations. This work presents an assessment approach to evaluate Quercus suber suitability to its plantation which is based on a random forest algorithm (RF). In fact, this suitability has been assessed through analyzing management data related to previous plantation success rates (SR). Then a relationship between SR and a set of environmental and social factors has been investigated using the RF. Application of the fitted model to continuous maps of all involved factors enabled establishment of suitability maps which would help managers to make more rational decisions in terms of cork oak regeneration, ensuring Maamora forest sustainability.展开更多
文摘The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting;small white embryos appear on the surface of calluses.
文摘Cork oak in Maamora forest is experiencing the dieback phenomenon. The evaluation of the latter in this forest has gained the importance over time and with the solicitation of managers to objectify its phytosanitary situation. Aiming at prioritizing management actions, remote sensing seems to be an effective tool to inquire about stands’ health conditions and their evolution. To this end, this study aims at mapping and validating health status of cork oak stands in Maamora. Sentinel 2 images in 2015 and 2020 were processed to calculate the differential normalized difference water index (NDWI), revealing vegetation moisture variation caused by drought. A statistical method based on thresholds was used to map cork oak dieback stands, those with no changes and those recovered. Results have shown that 54.63% of cork oak in Maamora forest have not changed in terms of phytosanitary situation between 2015 and 2020, 31.10% of oak stands are afflicted by a slight decline and 12.97% by a severe decline. Areas with slight or strong recovery remain minimal and represent 1.04% and 0.25% respectively. Ground data indicated that the map generated displayed a good distinction between stands severely and slightly declined with a global accuracy of 66.66%. Therefore, further research elaborating an advanced vegetation index reflecting the various factors of dieback would be of much importance.
文摘In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N30K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.
文摘The mineralization of the organic matter is a very important phenomenon which leads to the release of nutriments used by plants. The rate of transformation of the organic matter depends on several factors and parameters such as climatic factors and biological and physicochemical properties of the soil and the litter. In this study, we investigate the effect of the addition of litter of various species as well as the effect of soil moisture on mineralization of organic matter and on mineral nitrogen release in three soils sampled in three cork oak stands during a period of 41 days under the same laboratory conditions. Carbon mineralization was determined using CO2 respiration method, whereas the mineral nitrogen content was measured at the last day of incubation both in soil samples with added plant leaf material, and in control soil samples without addition under two treatments of moisture (40% and 80% WHC—water holding capacity). Our results show that the addition of leaf litter causes an increase in the microbial activity. Soils without addition were significantly different from the plant leaf added soils in respect to carbon mineralization at the end of the incubation period. Moreover, it is noted that the mineralization of carbon is more marked with moisture at 80% than that of 40%, contrary to that of the nitrogen, which is not influenced by the variation of moisture.
文摘Maamora is considered the most important cork-oak forest in the world with regard to surface. Therefore, anthropic pressure, including cork harvesting, grazing and soft acorn picking up by local communities, has harmful consequences on forest regeneration and the forest become older exceeding harvesting age. Thus, its sustainability depends on the managers’ ability to succeed cork oak plantations. This work presents an assessment approach to evaluate Quercus suber suitability to its plantation which is based on a random forest algorithm (RF). In fact, this suitability has been assessed through analyzing management data related to previous plantation success rates (SR). Then a relationship between SR and a set of environmental and social factors has been investigated using the RF. Application of the fitted model to continuous maps of all involved factors enabled establishment of suitability maps which would help managers to make more rational decisions in terms of cork oak regeneration, ensuring Maamora forest sustainability.