In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic em...In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N30K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.展开更多
The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes sho...The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting;small white embryos appear on the surface of calluses.展开更多
This paper observed through SEM and analyzed the microstructure of cork samples.The mechanical property,processing and utilization of cork is closely related to its microstructure.In the tangential section,cork cells ...This paper observed through SEM and analyzed the microstructure of cork samples.The mechanical property,processing and utilization of cork is closely related to its microstructure.In the tangential section,cork cells are seen as polygons in a honeycomb-like arrangement,while the transverse and radial sections show a brickwall-like structure.Each of these cork cells has average 14 faces.There are corrugations on the cell walls.The three dimension structure of cork cell is a prism,cork cells are connected with lentoid lenticels.展开更多
以秦巴山区栓皮栎软木为原料,对其细胞结构、基本物理特性及化学组成进行研究,以期为栓皮栎软木的预处理及高附加值的应用提供基础数据。通过场发射扫描电镜观察软木的微观结构,利用图像分析软件(Image-Pro Plus 6.0)测量软木细胞的特...以秦巴山区栓皮栎软木为原料,对其细胞结构、基本物理特性及化学组成进行研究,以期为栓皮栎软木的预处理及高附加值的应用提供基础数据。通过场发射扫描电镜观察软木的微观结构,利用图像分析软件(Image-Pro Plus 6.0)测量软木细胞的特征参数;按照国家和行业相关标准测量栓皮栎软木的主要物理性能指标和化学组成。结果表明,栓皮栎初生和再生软木细胞是沿树径向排列紧密的薄壁细胞,无细胞间隙,细胞壁均存在明显褶皱,在横切面上,初生和再生栓皮栎的早软木细胞壁平均厚度分别为1.10μm和1.02μm,晚软木的细胞壁平均厚度分别为1.52μm和1.99μm;在试验范围内,初生和再生软木的厚度测量值范围分别为6.00~18.92mm和8.55~23.07m,皮层厚度测量值范围分别为0.31~2.21mm和0.42~1.59mm,密度分别为0.197~0.355g·cm^(-3)和0.198~0.344g·cm^(-3)。解除压力15min后,初生和再生栓皮栎软木的径向压缩回弹率均值分别为83.16%和85.86%,解除压力24h后的径向压缩回弹率均值分别为88.42%和90.82%;初生和再生栓皮栎软木的主要化学成分有抽提物、软木脂、酸不溶木质素、综纤维素和灰分,其质量分数分别为12.79%和11.52%、36.34%和40.41%、20.12%和18.40%、18.29%和21.75%、2.44%和1.08%,软木脂是栓皮栎软木细胞壁的主要成分。再生软木的主要物理特性总体上优于初生软木,两者化学组成含量的差异是其物理性能差异的主要原因之一。展开更多
文摘In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N30K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.
文摘The present study is about in vitro culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting;small white embryos appear on the surface of calluses.
文摘This paper observed through SEM and analyzed the microstructure of cork samples.The mechanical property,processing and utilization of cork is closely related to its microstructure.In the tangential section,cork cells are seen as polygons in a honeycomb-like arrangement,while the transverse and radial sections show a brickwall-like structure.Each of these cork cells has average 14 faces.There are corrugations on the cell walls.The three dimension structure of cork cell is a prism,cork cells are connected with lentoid lenticels.
文摘以秦巴山区栓皮栎软木为原料,对其细胞结构、基本物理特性及化学组成进行研究,以期为栓皮栎软木的预处理及高附加值的应用提供基础数据。通过场发射扫描电镜观察软木的微观结构,利用图像分析软件(Image-Pro Plus 6.0)测量软木细胞的特征参数;按照国家和行业相关标准测量栓皮栎软木的主要物理性能指标和化学组成。结果表明,栓皮栎初生和再生软木细胞是沿树径向排列紧密的薄壁细胞,无细胞间隙,细胞壁均存在明显褶皱,在横切面上,初生和再生栓皮栎的早软木细胞壁平均厚度分别为1.10μm和1.02μm,晚软木的细胞壁平均厚度分别为1.52μm和1.99μm;在试验范围内,初生和再生软木的厚度测量值范围分别为6.00~18.92mm和8.55~23.07m,皮层厚度测量值范围分别为0.31~2.21mm和0.42~1.59mm,密度分别为0.197~0.355g·cm^(-3)和0.198~0.344g·cm^(-3)。解除压力15min后,初生和再生栓皮栎软木的径向压缩回弹率均值分别为83.16%和85.86%,解除压力24h后的径向压缩回弹率均值分别为88.42%和90.82%;初生和再生栓皮栎软木的主要化学成分有抽提物、软木脂、酸不溶木质素、综纤维素和灰分,其质量分数分别为12.79%和11.52%、36.34%和40.41%、20.12%和18.40%、18.29%和21.75%、2.44%和1.08%,软木脂是栓皮栎软木细胞壁的主要成分。再生软木的主要物理特性总体上优于初生软木,两者化学组成含量的差异是其物理性能差异的主要原因之一。
