Objective： To investigate the cardio-protective effects of Corocalm （疏冠胶囊） on acute myocardial ischemia in rats, and to explore its possible therapeutic mechanisms. Methods： The acute ischemic model was prepar...Objective： To investigate the cardio-protective effects of Corocalm （疏冠胶囊） on acute myocardial ischemia in rats, and to explore its possible therapeutic mechanisms. Methods： The acute ischemic model was prepared by ligating the left anterior descending （LAD） coronary artery in rats. The animals were divided into 6 groups, 8 in each group. The sham operated group underwent heart exposure without ligation and were treated with normal saline 3 ml/kg, while the other 5 groups, the model groups, consisted of acceptable acute ischemic model rats and were also treated with normal saline, with the Guanxin Capsule （ 冠心胶囊, GXC） group treated with refined GXC, 600 mg/kg, the low and high dose Corocalm groups treated with 85 mg/kg and 340 mg/kg of Corocalm respectively, and the Diltiazem group, treated with Diltiazem 5 mg/kg, with all the tested drugs prepared with normal saline into equal volume （ 3 ml/kg） and administrated once via duodenum 10 rain before ligation. Myocardial infarction area was determined by the quantitative histological assay with nitroblue tetrazolium （N-BT） stain. And the levels of creatine phosphokinase （CK）, lactate dehydrogenase （LDH）, malondialdehyde （MDA） content, and the activity of superoxide dismutase （SOD） in serum were measured by biochemical assay and spectrophotometry respectively. Besides, the blood viscosity in another 50 rats was determined, who received for 7 successive days oral administration with different concentration of Corocalm or aspirin. Results： It showed that low and high dose Corocalm could significantly reduce the infarction area, inhibit the increase of serum OK, LDH activity and MDA content, and enhance the SOD activity after ischemia/reperfusion. The whole blood viscosity at different shear rates in rats treated with high dose Corocalm was significantly lower than those treated with normal saline （P 0.05）. Conclusion： Corocalm has favourable protective effects on heart in ischemic condition, the effect of which might be through its actions in inhibiting CK and LDH activity, scavenging oxygen free radicals, and lowering blood viscosity.展开更多
Objective： To investigate the effects of Corocalm （Shuguan Capsule, 疏冠胶囊） on acute myocardial ischemia in anesthetized dogs and its possible therapeutic mechanism. Methods： The acute ischemia model was establi...Objective： To investigate the effects of Corocalm （Shuguan Capsule, 疏冠胶囊） on acute myocardial ischemia in anesthetized dogs and its possible therapeutic mechanism. Methods： The acute ischemia model was established by ligating the left anterior descending （LAD） artery. Twenty- five dogs were randomly divided into 5 groups （5 dogs in each group）： the control group （treated with normal saline 3 mL/kg）, the refined Guanxin Capsule group （精制冠心胶囊, GXC 200 mg/kg）, high and low dose Corocalm groups （48.5 mg/kg for low dose group and 194.0 mg/kg for high dose group） and the Diltiazem group （5 mg/kg）. The animals were treated via a single duodenal administration after the model was established. The experiments used epicardial electrocardiogram （EECG） to measure the scope and degree of myocardial ischemia. Simultaneously, the coronary blood flow （CBF） and serum activity levels of creatine phosphokinase （CK） and lactate dehydrogenase （LDH） were measured by electromagnetic flow meter and automatic biochemical analyzer respectively. The plasma endothelin （ET） content was quantified by radioimmunoassay. Results： Corocalm （48.5 mg/kg and 194.0 mg/kg） significantly decreased the degree and scope of myocardial ischemia, reduced the infarct area, markedly increased the CBF, and inhibited the increase of CK and LDH activities and ET levels induced by myocardial ischemia/infarction. Conclusion： Corocalm could improve the state of acute myocardial ischemia and infarction in dogs. The mechanism of action might be correlated to increasing CBF, inhibiting CK and LDH activities and preventing ET release.展开更多
基金Supported by the National Key Technology Programs Foundation during the 9th Five-year Plan Period (No. 96-903-01-02)
文摘Objective： To investigate the cardio-protective effects of Corocalm （疏冠胶囊） on acute myocardial ischemia in rats, and to explore its possible therapeutic mechanisms. Methods： The acute ischemic model was prepared by ligating the left anterior descending （LAD） coronary artery in rats. The animals were divided into 6 groups, 8 in each group. The sham operated group underwent heart exposure without ligation and were treated with normal saline 3 ml/kg, while the other 5 groups, the model groups, consisted of acceptable acute ischemic model rats and were also treated with normal saline, with the Guanxin Capsule （ 冠心胶囊, GXC） group treated with refined GXC, 600 mg/kg, the low and high dose Corocalm groups treated with 85 mg/kg and 340 mg/kg of Corocalm respectively, and the Diltiazem group, treated with Diltiazem 5 mg/kg, with all the tested drugs prepared with normal saline into equal volume （ 3 ml/kg） and administrated once via duodenum 10 rain before ligation. Myocardial infarction area was determined by the quantitative histological assay with nitroblue tetrazolium （N-BT） stain. And the levels of creatine phosphokinase （CK）, lactate dehydrogenase （LDH）, malondialdehyde （MDA） content, and the activity of superoxide dismutase （SOD） in serum were measured by biochemical assay and spectrophotometry respectively. Besides, the blood viscosity in another 50 rats was determined, who received for 7 successive days oral administration with different concentration of Corocalm or aspirin. Results： It showed that low and high dose Corocalm could significantly reduce the infarction area, inhibit the increase of serum OK, LDH activity and MDA content, and enhance the SOD activity after ischemia/reperfusion. The whole blood viscosity at different shear rates in rats treated with high dose Corocalm was significantly lower than those treated with normal saline （P 0.05）. Conclusion： Corocalm has favourable protective effects on heart in ischemic condition, the effect of which might be through its actions in inhibiting CK and LDH activity, scavenging oxygen free radicals, and lowering blood viscosity.
基金Supported by the National Key Technology Program Foundation during the 9th Five-Year Plan Period (No.96-903-01-02)
文摘Objective： To investigate the effects of Corocalm （Shuguan Capsule, 疏冠胶囊） on acute myocardial ischemia in anesthetized dogs and its possible therapeutic mechanism. Methods： The acute ischemia model was established by ligating the left anterior descending （LAD） artery. Twenty- five dogs were randomly divided into 5 groups （5 dogs in each group）： the control group （treated with normal saline 3 mL/kg）, the refined Guanxin Capsule group （精制冠心胶囊, GXC 200 mg/kg）, high and low dose Corocalm groups （48.5 mg/kg for low dose group and 194.0 mg/kg for high dose group） and the Diltiazem group （5 mg/kg）. The animals were treated via a single duodenal administration after the model was established. The experiments used epicardial electrocardiogram （EECG） to measure the scope and degree of myocardial ischemia. Simultaneously, the coronary blood flow （CBF） and serum activity levels of creatine phosphokinase （CK） and lactate dehydrogenase （LDH） were measured by electromagnetic flow meter and automatic biochemical analyzer respectively. The plasma endothelin （ET） content was quantified by radioimmunoassay. Results： Corocalm （48.5 mg/kg and 194.0 mg/kg） significantly decreased the degree and scope of myocardial ischemia, reduced the infarct area, markedly increased the CBF, and inhibited the increase of CK and LDH activities and ET levels induced by myocardial ischemia/infarction. Conclusion： Corocalm could improve the state of acute myocardial ischemia and infarction in dogs. The mechanism of action might be correlated to increasing CBF, inhibiting CK and LDH activities and preventing ET release.
