Objective: To study the regulating effect of corosoic acid on gene expression in placental tissue of rats with gestational diabetes mellitus and its protective effect on islet β cells. Methods:SD female rats were div...Objective: To study the regulating effect of corosoic acid on gene expression in placental tissue of rats with gestational diabetes mellitus and its protective effect on islet β cells. Methods:SD female rats were divided into corosoric acid group (CA group), gestational diabetes mellitus group (GDM group) and control group, CA group and GDM group were established into GDM models, and CA group were given corosoric acid intervention. At 20 d of gestation, the expression of insulin signal transducers and inflammatory reaction molecules in placenta and the expression of apoptosis molecules in islet were measured. Results: p-IRS1, p-IRS2, p-PI3K, GLUT1, GLUT3, GLUT4 and visfatin protein expression in placenta tissue as well as Bcl-2 mRNA expression in islet tissue of GDM group were significantly lower than those of control group whereas NF-kB, ICAM-1, TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression in islet tissue were significantly higher than those of control group;p-IRS1, p-IRS2, p-PI3K, GLUT1, GLUT3, GLUT4 and visfatin protein expression in placenta tissue as well as Bcl-2 mRNA expression in islet tissue of CA group were significantly higher than those of GDM group whereas NF-kB, ICAM-1, TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression in islet tissue were significantly lower than those of GDM group. Conclusion:Corosoic acid can improve the insulin resistance and reduce the islet β cell injury in rats with gestational diabetes mellitus.展开更多
文摘Objective: To study the regulating effect of corosoic acid on gene expression in placental tissue of rats with gestational diabetes mellitus and its protective effect on islet β cells. Methods:SD female rats were divided into corosoric acid group (CA group), gestational diabetes mellitus group (GDM group) and control group, CA group and GDM group were established into GDM models, and CA group were given corosoric acid intervention. At 20 d of gestation, the expression of insulin signal transducers and inflammatory reaction molecules in placenta and the expression of apoptosis molecules in islet were measured. Results: p-IRS1, p-IRS2, p-PI3K, GLUT1, GLUT3, GLUT4 and visfatin protein expression in placenta tissue as well as Bcl-2 mRNA expression in islet tissue of GDM group were significantly lower than those of control group whereas NF-kB, ICAM-1, TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression in islet tissue were significantly higher than those of control group;p-IRS1, p-IRS2, p-PI3K, GLUT1, GLUT3, GLUT4 and visfatin protein expression in placenta tissue as well as Bcl-2 mRNA expression in islet tissue of CA group were significantly higher than those of GDM group whereas NF-kB, ICAM-1, TNF-α and IL-1β protein expression in placenta tissue as well as Bax and Caspase-3 mRNA expression in islet tissue were significantly lower than those of GDM group. Conclusion:Corosoic acid can improve the insulin resistance and reduce the islet β cell injury in rats with gestational diabetes mellitus.
