B7-H1, a recently described member of the B7 family of costimulatory molecules, is thought to be involved in tumor immune escape by inducing T-cell apoptosis. In order to investigate the relationship between B7-H1 and...B7-H1, a recently described member of the B7 family of costimulatory molecules, is thought to be involved in tumor immune escape by inducing T-cell apoptosis. In order to investigate the relationship between B7-H1 and immune escape of bladder cancer, B7-H1 expression in 50 cases of bladder cancer was detected by using immunohistochemical method. Survival curves were con- structed using the Kaplan-Meier method and independent prognostic factors were evaluated using the Cox regression model. Our results showed that the positive rate of B7-H1 immunostaining in normal bladder tissue and bladder cancer was 0 and 72% respectively. The expression of B7-H1 was strongly associated with the pathological grade, clinical stage and recurrence (P〈0.05). The survival rate was significantly lower in patients with B7-H1 positive group than in those with B7-H1 negative group and multi-variable analysis revealed that B7-H1 could be regarded as an independent factor in evaluating the prognosis of bladder cancer. It is concluded that the expression of B7-H1 is strongly associated with neoplastic progression and prognosis of bladder cancer. The manipulation of B7-H1 may become a beneficial target for immunotherapy in human bladder cancer.展开更多
用等压法研究了298.15 K下L iC l-L i2B4O7-H2O体系在不同L iB4O7质量摩尔浓度时的等压平衡浓度,水活度;计算了L iC l和L i2B4O7混合盐溶液的渗透系数等热力学性质.用298.15 K下的实验数据对P itzer离子相互作用模型进行了参数化研究,...用等压法研究了298.15 K下L iC l-L i2B4O7-H2O体系在不同L iB4O7质量摩尔浓度时的等压平衡浓度,水活度;计算了L iC l和L i2B4O7混合盐溶液的渗透系数等热力学性质.用298.15 K下的实验数据对P itzer离子相互作用模型进行了参数化研究,拟合求取了298.15 K下P itzer离子相互作用参数,用获得的参数计算了L iC l和L i2B4O7在L iC l-L i2B4O7-H2O体系中的活度系数.P itzer模型计算的渗透系数值与实验结果一致.展开更多
Objective: To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepa...Objective: To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepatitis B virus infection. Methods: Thirty HBV infected patients in the immunoreactive phase and 20 patients in the immunotolerant phase were enrolled in the study, while 20 healthy volunteers were used as controls. RT- PCR and real-time PCR methods were used to detect the expression levels of B7-2 and PD-L1 mRNA in peripheral blood mononuclear cells in chronic HBV infected patients. Results: The B7-2 expression in irnrnunoreactive and immunotolerant patients was significantly lower than that in the controls (P all 〈 0.01 ); B7-2 expression in immunoreactive patients was significantly lower than in immunotolerant patients (P 〈 0.01). PD-L1 expression in irnmunoreactive patients and immunotolerant patients was significantly higher than that in normal controls (P all 〈 0.01). The PD-L1/BT-2 ratios in immunoreactive and immunotolerant patients were significantly higher than that of the healthy controls (P all 〈 0.01); the PD-L1/ B7-2 ratio was significantly higher in the immunoreactive patients than in the immunotolerant patients (P 〈 0.01). Conclusion: In chronic HBV infection, changes in the expression of co-stimulatory and co-inhibitory molecules imply a protective adjustment against the patient' s immune response that may result in increased immunotolerance and persistent HBV infection.展开更多
基金supported by a grant from Hubei Provin-cial Science and Technology Key Program Foundation of China (No. 2007AA402C60).
文摘B7-H1, a recently described member of the B7 family of costimulatory molecules, is thought to be involved in tumor immune escape by inducing T-cell apoptosis. In order to investigate the relationship between B7-H1 and immune escape of bladder cancer, B7-H1 expression in 50 cases of bladder cancer was detected by using immunohistochemical method. Survival curves were con- structed using the Kaplan-Meier method and independent prognostic factors were evaluated using the Cox regression model. Our results showed that the positive rate of B7-H1 immunostaining in normal bladder tissue and bladder cancer was 0 and 72% respectively. The expression of B7-H1 was strongly associated with the pathological grade, clinical stage and recurrence (P〈0.05). The survival rate was significantly lower in patients with B7-H1 positive group than in those with B7-H1 negative group and multi-variable analysis revealed that B7-H1 could be regarded as an independent factor in evaluating the prognosis of bladder cancer. It is concluded that the expression of B7-H1 is strongly associated with neoplastic progression and prognosis of bladder cancer. The manipulation of B7-H1 may become a beneficial target for immunotherapy in human bladder cancer.
文摘用等压法研究了298.15 K下L iC l-L i2B4O7-H2O体系在不同L iB4O7质量摩尔浓度时的等压平衡浓度,水活度;计算了L iC l和L i2B4O7混合盐溶液的渗透系数等热力学性质.用298.15 K下的实验数据对P itzer离子相互作用模型进行了参数化研究,拟合求取了298.15 K下P itzer离子相互作用参数,用获得的参数计算了L iC l和L i2B4O7在L iC l-L i2B4O7-H2O体系中的活度系数.P itzer模型计算的渗透系数值与实验结果一致.
文摘Objective: To explore the roles of the expression of the co-stimulatory molecule, B7-2, and the co-inhibitory molecule, PD-L1, on peripheral blood mononuclear cells in the mechanism of immunotolerance in chronic hepatitis B virus infection. Methods: Thirty HBV infected patients in the immunoreactive phase and 20 patients in the immunotolerant phase were enrolled in the study, while 20 healthy volunteers were used as controls. RT- PCR and real-time PCR methods were used to detect the expression levels of B7-2 and PD-L1 mRNA in peripheral blood mononuclear cells in chronic HBV infected patients. Results: The B7-2 expression in irnrnunoreactive and immunotolerant patients was significantly lower than that in the controls (P all 〈 0.01 ); B7-2 expression in immunoreactive patients was significantly lower than in immunotolerant patients (P 〈 0.01). PD-L1 expression in irnmunoreactive patients and immunotolerant patients was significantly higher than that in normal controls (P all 〈 0.01). The PD-L1/BT-2 ratios in immunoreactive and immunotolerant patients were significantly higher than that of the healthy controls (P all 〈 0.01); the PD-L1/ B7-2 ratio was significantly higher in the immunoreactive patients than in the immunotolerant patients (P 〈 0.01). Conclusion: In chronic HBV infection, changes in the expression of co-stimulatory and co-inhibitory molecules imply a protective adjustment against the patient' s immune response that may result in increased immunotolerance and persistent HBV infection.