The present study was conducted to analyze cotton cultivar and location differences in motes, and to determine the relationships among these and ginning outturn. Therefore, the seed cotton of five promising hybrids na...The present study was conducted to analyze cotton cultivar and location differences in motes, and to determine the relationships among these and ginning outturn. Therefore, the seed cotton of five promising hybrids namely;G.77 × Pima S6 and G.84 × (G.74 × G.68) growing in [Kafr El-Sheikh - Kafr El-Dawar - Etay El-Barood - Damietta], G.89 × Pima S6 growing in [El-Sharkia - El-Gharbiya - El-Dakahliya - El-Monofiya], G.83 × (G.75 × 5844) × G.80 and G.90 × Australian growing in [Sohag - El-Minia - Beni-Sueif - El-Faium] were used in this study. The results obtained indicate that the varieties exhibited different behavior responses to environmental conditions. On the whole, environmental factors associated with differences in place of growth, appeared to have much more influence on the number of motes than did varietal factors. Most of the locks for the promising hybrids at the different locations tend to cluster around the mean of 6 or 7 seeds per lock. There is a fairly marked tendency for the lock index, lint weight and lint percentage to decrease as the motes percentage increase. On the other hand, most of the promising hybrids under study tend to increase in the seed index as the motes percentage increase. However, the increasing in seed index as a result of the increasing in motes percentage for some cotton cultivars growing at different environments could be explain the difference in behavior for these cotton cultivars in lint percentage.展开更多
[Objective] This study was conducted to evaluate the efficiency of somatic embryogenesis and plant regeneration for an upland cotton cultivar Xinluzao33 under the induction of different hormone combinations and thus t...[Objective] This study was conducted to evaluate the efficiency of somatic embryogenesis and plant regeneration for an upland cotton cultivar Xinluzao33 under the induction of different hormone combinations and thus to determine the optimal hormone combination. [Method] Calli of Xinluzao33(Gossypium hirsutum L.) were induced from seedling hypocotyl tissue by a range of DK and BK combinations. Embryogenic calli and embryos were induced on callus-inducing medium(CIM) without any hormones. Callus appearance and quality were compared to determine which medium was the optimal for callus induction. Embryogenesis ratio was calculated to determine which medium was the best for somatic embryogenesis and plant regeneration. [Result] Callus induction rate was 100% in all the 12 hormone combinations.The calli were yellow or kelly, and their texture was loose or soft under low concentrations of DK combinations, green or white, variably compact under high concentrations of DK combinations. The calli induced by BK combinations were kelly or green, covering creamy white substance. The best medium for callus induction was DK6(0.05 mg/L 2, 4-D and 0.10 mg/L KT). Embryogenic calli were successfully induced from all the combinations. The efficiency of embryogenic callus induction,embryogenesis, and plantlet regeneration were significantly different among the 12 combinations. The result showed that the embryogenesis ratio was the highest in BK3 combination(0.50 mg/L IBA and 0.50 mg/L KT), 72.86% of embryogenic calli differentiated into somatic embryos after being cultured on CIM for 80 d, and80.93% of the somatic embryos finally regenerated into plants on SEM(somatic embryo induction medium). [Conclusion] These results indicate that hormone combination BK3(0.50 mg/LIBA and 0.50 mg/L KT) was the best medium for somatic embryogenesis and plant regeneration from Xinluzao33.展开更多
Life table parameters of the cotton aphid, Aphis gossypii Glover, were estimated on five cotton cultivars (‘ Sealand' ,‘Siokra' ,' Vararnin' ,‘ Bakhtegan' and ‘ Sahel' ). Demographic parameters of the cott...Life table parameters of the cotton aphid, Aphis gossypii Glover, were estimated on five cotton cultivars (‘ Sealand' ,‘Siokra' ,' Vararnin' ,‘ Bakhtegan' and ‘ Sahel' ). Demographic parameters of the cotton aphid were assessed at 27.5 ± 1 ℃, 65% + 10% RH and a photoperiod of 14:10 (L: D)h. The shortest developmental time for the nymphal stages was 5.5 days on ‘Siokra' and the longest was 6.1 days on‘ Sealand'. The highest offspring per female was 29.6 on ‘Sahel' and the lowest was 15.3 on ‘Sealand'. The rm values varied from 0.272 on ‘Sealand' to 0.382 (day^-1) on ‘Varamin'. Jackknife estimates of the A. gossypii parameters on these cultivars indicated the greatest developmental rate and fecundity on ‘ Varamin' and the poorest on ‘ Sealand' cultivar.展开更多
[Objective] This study was to analyze the high yielding, stability, adaptabil-ity and main characteristics of check cultivars in national cotton regional trials in the Yangtze River Val ey in 2000-2013, with the aim t...[Objective] This study was to analyze the high yielding, stability, adaptabil-ity and main characteristics of check cultivars in national cotton regional trials in the Yangtze River Val ey in 2000-2013, with the aim to provide theoretical basis for the reasonable selection of check cultivars for cotton regional trials. [Method] GGE biplot analysis method was used to carry out the intensive analysis and comprehensive comparisons on the performances and differences of the 9 major characters of check cultivars, namely, lint cotton yield , stability, and other main traits including bol weight, bol number, lint percentage, fiber length, fiber strength, micronaire val-ue, plant height and seed index. [Result] Ezamian 10 was suitable in the majority of cotton planting regions in the Yangtze River Val ey, with the best high yielding abili-ty and perfect yield stability. The yielding ability of Xiangzamian 8 ranked the sec-ond, but its stability was the poorest. Xiangzamian 8 was of the specific adaptive cultivar with relatively good performance in high yielding but the poorest stability. The suitable planting areas of Xiangzamian 8 were limited to the cotton planting re-gion around Dongting Lake in Hunan province, Poyang Lake in Jiangxi Province, the Coastal region in Jiangsu Province and Wuhan district in Hubei Province. Xi-angzamian 2 performanced poorer in both yielding and stability, while Simian 3 ranked first in stability but the last in yielding ability. The dominant character of Xi-angzamian 8 and Xiangzamian 2 were high cotton plants and big bol s. Simian 3 performanced super in lint percentage and fiber length. Ezamian 10 was strong in cotton bol formation ability, lint cotton yielding, fiber strength and seed size, as wel as high micronaire value. [Conclusion] The outstanding performance of Ezamian 10 in high and stable yield in the Yangtze River Val ey in recent years has effectively promoted the high yielding ability of candidate varieties in cotton regional trials in the area.展开更多
Background: Within-canopy interception of photosynthetically active radiation(PAR) impacts yield and other agronomic traits in cotton(Gossypium hirsutum L.). Field experiments were conducted to investigate the influen...Background: Within-canopy interception of photosynthetically active radiation(PAR) impacts yield and other agronomic traits in cotton(Gossypium hirsutum L.). Field experiments were conducted to investigate the influence of 6 cotton varieties(they belong to 3 different plant types) on yield, yield distribution, light interception(LI), LI distribution and the relationship between yield formation and LI in Anyang, Henan, in 2014 and 2015.Result: The results showed that cotton cultivars with long branches(loose-type) intercepted more LI than did cultivars with short branches(compact-type), due to increased LI in the middle and upper canopy. Although loose-type varieties had greater LI, they did not yield significantly higher than compact-type varieties, due to decreased harvest index. Therefore, improving the harvest index by adjusting the source-to-sink relationship may further increase cotton yield for loose-type cotton. In addition, there was a positive relationship between reproductive organ biomass accumulation and canopy-accumulated LI, indicating that enhancing LI is important for yield improvement for each cultivar. Furthermore, yield distribution within the canopy was significantly linearly related to vertical LI distribution.Conclusion: Therefore, optimizing canopy structure of different plant type and subsequently optimizing LI distribution within the cotton canopy can effectively enhance the yield.展开更多
Cotton growth and development is effected by various ecological issues like temperature fluctuations, distribution and quantity of rainfall, relative humidity and winds which are the climate change attributes. A field...Cotton growth and development is effected by various ecological issues like temperature fluctuations, distribution and quantity of rainfall, relative humidity and winds which are the climate change attributes. A field experiment was carried out to find out the response of cotton to weather variables in terms of total variation in yield and quality. The effect of planting times and thermal temperatures (cumulative heat units) on yield of 4 cotton cultivars viz;CIM-600, CIM-616, CIM-622 & CRIS-641 was evaluated. Plants were sown on 6 planting times during the year 2015-2016 and 2016-2017 in an experiment conducted in randomized complete block design having three replications. Cotton cultivars depicted significant variances for number of bolls plant-1, boll weight and seed cotton yield. The cultivar CIM-616 depicted the highest seed cotton yield of 2083.60 kg·ha-1 on interpretation of highest bolls and boll weight. Maximum seed cotton yield was noted in planting time from 1st April to 15th April whereas early and late planting decreases the seed cotton yield on account of less accretion of cumulative heat units. Regression analysis depicted that rise of one unit (15 days) from early to optimal date (15th March to 15th April) enhanced the seed cotton yield by 93.76 kg·ha-1 (y = -93.764x2 + 521.04x + 1364). Delayed planting also reduces the seed cotton yield with the same ratio. It is therefore established that cotton must be cultivated from 1st April to 1st May to harvest good production in this type of climate.展开更多
The cotton leafworm is an important defoliating pest of cotton in Brazil, and occurs in all regions where the crop is cultivated. The purpose of this study was to evaluate the effect of larval densities of A. argillac...The cotton leafworm is an important defoliating pest of cotton in Brazil, and occurs in all regions where the crop is cultivated. The purpose of this study was to evaluate the effect of larval densities of A. argillacea after different infestation periods on the yield components of four cotton cultivars. The experiment was carried out in Pindorama, SP, Brazil, in the 2008/2009 growing season. The cultivars IAC-25, DeltaOPAL, Fibermax 966 and Fibermax 993 were artificially infested with A. argillacea larvae at three times (30, 60 and 90 DAE) and four densities (0, 2, 4, and 6 larvae per plant). The average boll weight (g), fiber percentage (%), 100-seed weight (g) and yield (kg·ha-1) were evaluated. With increasing infestation density of A. argillacea, the cotton yield of the cultivars decreased. The presence of larvae significantly reduced the weight of 100 seeds of cultivar Fibermax 966. Initial infestations reduced the boll weight of IAC-25 and DeltaOPAL, while cultivars Fibermax 966 and Fibermax 993 were most affected by late infestations. Early infestation compromised fiber percentage of cultivar DeltaOPAL and late infestations were most harmful to cultivar Fibermax 966. Early A. argillacea infestation reduced the yield of DeltaOPAL, while infestations 60 DAE caused the most damage to IAC-25, and the other cultivars were not affected by the moment of infestation.展开更多
Sixteen cotton cultivars widely planted in China were sowed under five different drought concentrations(0,2.5,5,7.5,and 10%)using PEG6000 to screen the indices of drought resistance identification and explore the drou...Sixteen cotton cultivars widely planted in China were sowed under five different drought concentrations(0,2.5,5,7.5,and 10%)using PEG6000 to screen the indices of drought resistance identification and explore the drought resistance of different cotton cultivars.Eighteen physiological indices including root,stem,and leaf water contents(RWC,SWC,and LWC),net photosynthetic rate(Pn),the maximum photochemical quantum yield(Fv/Fm),the actual photochemical quantum yield(ΦPSII),non-photochemical quenching coefficient(NPQ),leaf water potential(LWP),osmotic potential(Ψs),leaf relative conductivity(REC),leaf proline content(Pro),leaf and root soluble protein contents(LSPC and RSPC),leaf and root malondialdehyde(MDA)contents(LMDA and RMDA),root superoxide dismutase,peroxidase,and catalase activities(RSOD,RPOD,and RCAT)were measured.Results indicated the 18 physiological indices can be converted into five or six independent comprehensive indices by principal component analysis,and nine typical indices(Fv/Fm,SWC,LWP,Pro,LMDA,RSPC,RMDA,RSOD,and RCAT)screened out by a stepwise regression method could be utilized to evaluate the drought resistance.Moreover,the 16 cotton cultivars were divided into four types:drought sensitive,drought weak sensitive,moderate drought resistant,and drought resistant types.The resistance ability of two selected cotton cultivars(drought resistant cultivar,Dexiamian 1;drought sensitive cultivar,Yuzaomian 9110)with contrasting drought sensitivities were further verified by pot experiment.Results showed that the responses of final cotton biomass,yield,and yield composition to drought were significantly different between the two cultivars.In conclusion,drought resistant cultivar Dexiamian 1 and drought sensitive cultivar Yuzaomian 9110 were screened through hydroponics experiment,which can be used as ideal experimental materials to study the mechanism of different cotton cultivars with contrasting drought sensitivities in response to drought stress.展开更多
【目的】采用SSR标记构建2008年中国3大棉区8个棉花主产省份32份棉花主栽品种的DNA指纹图谱并进行遗传多样性分析。【方法】从214对候选引物中筛选出36对多态性高、稳定性好且在染色体上分布均匀的引物作为核心引物,构建棉花主栽品种DN...【目的】采用SSR标记构建2008年中国3大棉区8个棉花主产省份32份棉花主栽品种的DNA指纹图谱并进行遗传多样性分析。【方法】从214对候选引物中筛选出36对多态性高、稳定性好且在染色体上分布均匀的引物作为核心引物,构建棉花主栽品种DNA指纹图谱。【结果】36对SSR引物在32份材料中共扩增出142种多态性基因型,每对引物的基因型从2—11种不等,平均每对引物扩增出3.94种基因型。9对引物在10个品种上具有特征带型。最少采用5对引物进行组合鉴定即可将32个棉花品种完全区分开。NTSYS-pc V 2.10软件分析表明,长江流域棉区品种间遗传差异最大,新疆棉区次之,黄河流域棉区最小。常规品种的遗传基础窄于杂交陆地棉。【结论】核心引物组合法相比特征谱带法更适用于构建中国棉花主栽品种DNA指纹图谱,品种间的亲缘关系与地理来源有一定的相关性。展开更多
文摘The present study was conducted to analyze cotton cultivar and location differences in motes, and to determine the relationships among these and ginning outturn. Therefore, the seed cotton of five promising hybrids namely;G.77 × Pima S6 and G.84 × (G.74 × G.68) growing in [Kafr El-Sheikh - Kafr El-Dawar - Etay El-Barood - Damietta], G.89 × Pima S6 growing in [El-Sharkia - El-Gharbiya - El-Dakahliya - El-Monofiya], G.83 × (G.75 × 5844) × G.80 and G.90 × Australian growing in [Sohag - El-Minia - Beni-Sueif - El-Faium] were used in this study. The results obtained indicate that the varieties exhibited different behavior responses to environmental conditions. On the whole, environmental factors associated with differences in place of growth, appeared to have much more influence on the number of motes than did varietal factors. Most of the locks for the promising hybrids at the different locations tend to cluster around the mean of 6 or 7 seeds per lock. There is a fairly marked tendency for the lock index, lint weight and lint percentage to decrease as the motes percentage increase. On the other hand, most of the promising hybrids under study tend to increase in the seed index as the motes percentage increase. However, the increasing in seed index as a result of the increasing in motes percentage for some cotton cultivars growing at different environments could be explain the difference in behavior for these cotton cultivars in lint percentage.
基金Supported by the National Transgenic Major Project of China(2009ZX08009-090B)the Biological Germplasm Project of Xinjiang Production&Construction Crops(2012BD046)the Technology Plan of Xinjiang Academy of Agricultural and Reclamation Sciences(81YYD201506)
文摘[Objective] This study was conducted to evaluate the efficiency of somatic embryogenesis and plant regeneration for an upland cotton cultivar Xinluzao33 under the induction of different hormone combinations and thus to determine the optimal hormone combination. [Method] Calli of Xinluzao33(Gossypium hirsutum L.) were induced from seedling hypocotyl tissue by a range of DK and BK combinations. Embryogenic calli and embryos were induced on callus-inducing medium(CIM) without any hormones. Callus appearance and quality were compared to determine which medium was the optimal for callus induction. Embryogenesis ratio was calculated to determine which medium was the best for somatic embryogenesis and plant regeneration. [Result] Callus induction rate was 100% in all the 12 hormone combinations.The calli were yellow or kelly, and their texture was loose or soft under low concentrations of DK combinations, green or white, variably compact under high concentrations of DK combinations. The calli induced by BK combinations were kelly or green, covering creamy white substance. The best medium for callus induction was DK6(0.05 mg/L 2, 4-D and 0.10 mg/L KT). Embryogenic calli were successfully induced from all the combinations. The efficiency of embryogenic callus induction,embryogenesis, and plantlet regeneration were significantly different among the 12 combinations. The result showed that the embryogenesis ratio was the highest in BK3 combination(0.50 mg/L IBA and 0.50 mg/L KT), 72.86% of embryogenic calli differentiated into somatic embryos after being cultured on CIM for 80 d, and80.93% of the somatic embryos finally regenerated into plants on SEM(somatic embryo induction medium). [Conclusion] These results indicate that hormone combination BK3(0.50 mg/LIBA and 0.50 mg/L KT) was the best medium for somatic embryogenesis and plant regeneration from Xinluzao33.
文摘Life table parameters of the cotton aphid, Aphis gossypii Glover, were estimated on five cotton cultivars (‘ Sealand' ,‘Siokra' ,' Vararnin' ,‘ Bakhtegan' and ‘ Sahel' ). Demographic parameters of the cotton aphid were assessed at 27.5 ± 1 ℃, 65% + 10% RH and a photoperiod of 14:10 (L: D)h. The shortest developmental time for the nymphal stages was 5.5 days on ‘Siokra' and the longest was 6.1 days on‘ Sealand'. The highest offspring per female was 29.6 on ‘Sahel' and the lowest was 15.3 on ‘Sealand'. The rm values varied from 0.272 on ‘Sealand' to 0.382 (day^-1) on ‘Varamin'. Jackknife estimates of the A. gossypii parameters on these cultivars indicated the greatest developmental rate and fecundity on ‘ Varamin' and the poorest on ‘ Sealand' cultivar.
基金Supported by National Key Special Project for Breeding and Cultivation of GMO Varieties in China(2012ZX08013016)the Special Project of Regional Crop Trials from the Ministry of Agriculture of China(2911108)
文摘[Objective] This study was to analyze the high yielding, stability, adaptabil-ity and main characteristics of check cultivars in national cotton regional trials in the Yangtze River Val ey in 2000-2013, with the aim to provide theoretical basis for the reasonable selection of check cultivars for cotton regional trials. [Method] GGE biplot analysis method was used to carry out the intensive analysis and comprehensive comparisons on the performances and differences of the 9 major characters of check cultivars, namely, lint cotton yield , stability, and other main traits including bol weight, bol number, lint percentage, fiber length, fiber strength, micronaire val-ue, plant height and seed index. [Result] Ezamian 10 was suitable in the majority of cotton planting regions in the Yangtze River Val ey, with the best high yielding abili-ty and perfect yield stability. The yielding ability of Xiangzamian 8 ranked the sec-ond, but its stability was the poorest. Xiangzamian 8 was of the specific adaptive cultivar with relatively good performance in high yielding but the poorest stability. The suitable planting areas of Xiangzamian 8 were limited to the cotton planting re-gion around Dongting Lake in Hunan province, Poyang Lake in Jiangxi Province, the Coastal region in Jiangsu Province and Wuhan district in Hubei Province. Xi-angzamian 2 performanced poorer in both yielding and stability, while Simian 3 ranked first in stability but the last in yielding ability. The dominant character of Xi-angzamian 8 and Xiangzamian 2 were high cotton plants and big bol s. Simian 3 performanced super in lint percentage and fiber length. Ezamian 10 was strong in cotton bol formation ability, lint cotton yielding, fiber strength and seed size, as wel as high micronaire value. [Conclusion] The outstanding performance of Ezamian 10 in high and stable yield in the Yangtze River Val ey in recent years has effectively promoted the high yielding ability of candidate varieties in cotton regional trials in the area.
基金funded by the National Natural Science Foundation of China(31371561)
文摘Background: Within-canopy interception of photosynthetically active radiation(PAR) impacts yield and other agronomic traits in cotton(Gossypium hirsutum L.). Field experiments were conducted to investigate the influence of 6 cotton varieties(they belong to 3 different plant types) on yield, yield distribution, light interception(LI), LI distribution and the relationship between yield formation and LI in Anyang, Henan, in 2014 and 2015.Result: The results showed that cotton cultivars with long branches(loose-type) intercepted more LI than did cultivars with short branches(compact-type), due to increased LI in the middle and upper canopy. Although loose-type varieties had greater LI, they did not yield significantly higher than compact-type varieties, due to decreased harvest index. Therefore, improving the harvest index by adjusting the source-to-sink relationship may further increase cotton yield for loose-type cotton. In addition, there was a positive relationship between reproductive organ biomass accumulation and canopy-accumulated LI, indicating that enhancing LI is important for yield improvement for each cultivar. Furthermore, yield distribution within the canopy was significantly linearly related to vertical LI distribution.Conclusion: Therefore, optimizing canopy structure of different plant type and subsequently optimizing LI distribution within the cotton canopy can effectively enhance the yield.
文摘Cotton growth and development is effected by various ecological issues like temperature fluctuations, distribution and quantity of rainfall, relative humidity and winds which are the climate change attributes. A field experiment was carried out to find out the response of cotton to weather variables in terms of total variation in yield and quality. The effect of planting times and thermal temperatures (cumulative heat units) on yield of 4 cotton cultivars viz;CIM-600, CIM-616, CIM-622 & CRIS-641 was evaluated. Plants were sown on 6 planting times during the year 2015-2016 and 2016-2017 in an experiment conducted in randomized complete block design having three replications. Cotton cultivars depicted significant variances for number of bolls plant-1, boll weight and seed cotton yield. The cultivar CIM-616 depicted the highest seed cotton yield of 2083.60 kg·ha-1 on interpretation of highest bolls and boll weight. Maximum seed cotton yield was noted in planting time from 1st April to 15th April whereas early and late planting decreases the seed cotton yield on account of less accretion of cumulative heat units. Regression analysis depicted that rise of one unit (15 days) from early to optimal date (15th March to 15th April) enhanced the seed cotton yield by 93.76 kg·ha-1 (y = -93.764x2 + 521.04x + 1364). Delayed planting also reduces the seed cotton yield with the same ratio. It is therefore established that cotton must be cultivated from 1st April to 1st May to harvest good production in this type of climate.
文摘The cotton leafworm is an important defoliating pest of cotton in Brazil, and occurs in all regions where the crop is cultivated. The purpose of this study was to evaluate the effect of larval densities of A. argillacea after different infestation periods on the yield components of four cotton cultivars. The experiment was carried out in Pindorama, SP, Brazil, in the 2008/2009 growing season. The cultivars IAC-25, DeltaOPAL, Fibermax 966 and Fibermax 993 were artificially infested with A. argillacea larvae at three times (30, 60 and 90 DAE) and four densities (0, 2, 4, and 6 larvae per plant). The average boll weight (g), fiber percentage (%), 100-seed weight (g) and yield (kg·ha-1) were evaluated. With increasing infestation density of A. argillacea, the cotton yield of the cultivars decreased. The presence of larvae significantly reduced the weight of 100 seeds of cultivar Fibermax 966. Initial infestations reduced the boll weight of IAC-25 and DeltaOPAL, while cultivars Fibermax 966 and Fibermax 993 were most affected by late infestations. Early infestation compromised fiber percentage of cultivar DeltaOPAL and late infestations were most harmful to cultivar Fibermax 966. Early A. argillacea infestation reduced the yield of DeltaOPAL, while infestations 60 DAE caused the most damage to IAC-25, and the other cultivars were not affected by the moment of infestation.
基金supported by the National Natural Science Foundation of China (31630051 and 31571606)the Jiangsu Collaborative Innovation Center for Modern Crop Production, China (JCIC-MCP)the earmarked fund for China Agriculture Research System (CARS-18-14)
文摘Sixteen cotton cultivars widely planted in China were sowed under five different drought concentrations(0,2.5,5,7.5,and 10%)using PEG6000 to screen the indices of drought resistance identification and explore the drought resistance of different cotton cultivars.Eighteen physiological indices including root,stem,and leaf water contents(RWC,SWC,and LWC),net photosynthetic rate(Pn),the maximum photochemical quantum yield(Fv/Fm),the actual photochemical quantum yield(ΦPSII),non-photochemical quenching coefficient(NPQ),leaf water potential(LWP),osmotic potential(Ψs),leaf relative conductivity(REC),leaf proline content(Pro),leaf and root soluble protein contents(LSPC and RSPC),leaf and root malondialdehyde(MDA)contents(LMDA and RMDA),root superoxide dismutase,peroxidase,and catalase activities(RSOD,RPOD,and RCAT)were measured.Results indicated the 18 physiological indices can be converted into five or six independent comprehensive indices by principal component analysis,and nine typical indices(Fv/Fm,SWC,LWP,Pro,LMDA,RSPC,RMDA,RSOD,and RCAT)screened out by a stepwise regression method could be utilized to evaluate the drought resistance.Moreover,the 16 cotton cultivars were divided into four types:drought sensitive,drought weak sensitive,moderate drought resistant,and drought resistant types.The resistance ability of two selected cotton cultivars(drought resistant cultivar,Dexiamian 1;drought sensitive cultivar,Yuzaomian 9110)with contrasting drought sensitivities were further verified by pot experiment.Results showed that the responses of final cotton biomass,yield,and yield composition to drought were significantly different between the two cultivars.In conclusion,drought resistant cultivar Dexiamian 1 and drought sensitive cultivar Yuzaomian 9110 were screened through hydroponics experiment,which can be used as ideal experimental materials to study the mechanism of different cotton cultivars with contrasting drought sensitivities in response to drought stress.
文摘【目的】采用SSR标记构建2008年中国3大棉区8个棉花主产省份32份棉花主栽品种的DNA指纹图谱并进行遗传多样性分析。【方法】从214对候选引物中筛选出36对多态性高、稳定性好且在染色体上分布均匀的引物作为核心引物,构建棉花主栽品种DNA指纹图谱。【结果】36对SSR引物在32份材料中共扩增出142种多态性基因型,每对引物的基因型从2—11种不等,平均每对引物扩增出3.94种基因型。9对引物在10个品种上具有特征带型。最少采用5对引物进行组合鉴定即可将32个棉花品种完全区分开。NTSYS-pc V 2.10软件分析表明,长江流域棉区品种间遗传差异最大,新疆棉区次之,黄河流域棉区最小。常规品种的遗传基础窄于杂交陆地棉。【结论】核心引物组合法相比特征谱带法更适用于构建中国棉花主栽品种DNA指纹图谱,品种间的亲缘关系与地理来源有一定的相关性。