Background: Earlier we have reported that the exclusion of solar UV-B increased the growth and yield of cotton plants as compared with ambient UV-B. The UV-B radiation effects on the saponins and the impact of ambient...Background: Earlier we have reported that the exclusion of solar UV-B increased the growth and yield of cotton plants as compared with ambient UV-B. The UV-B radiation effects on the saponins and the impact of ambient and reduced UV-B on the accumulation of saponins has not been investigated yet. Thus a field experiment was conducted to study the influence of solar UV-B on the growth response and saponin synthesis in cotton(Gossypium hirsutum) var. Vikram plants by the exclusion of UV-B. The cotton plants were grown in specially designed iron chambers, wrapped with filters that excluded UV-B(<315 nm), or transmitted ambient UV-B.Results: Exclusion of ambient UV-B enhanced the growth of cotton plants in terms of plant height and leaf area.Greater plant height in UV-B excluded plants was due to elongated internode and more number of nodes.Enhancement in growth was accompanied by a decrease in the accumulation of saponins, which was quantified by the spectrophotometric, TLC and HPLC methods. Solvent extraction of saponins from the internodes and leaves were used for the bioassay of Amaranthus hypocotyl growth and expansion of cucumber cotyledons. Extracts obtained from the UV-B excluded plants(leaves and internodes) promoted the growth to a larger extent as compared with the extracts obtained from ambient grown plants.Conclusions: The result indicates that solar UV-B may possibly suppress the plant growth by regulating the synthesis of natural growth inhibitor’s like saponin.展开更多
Dynamic acquisition of crop morphology is beneficial to real-time variable decision of precise spraying operations in fields.However,the existing spraying quantity regulation has high tolerance on the statistical char...Dynamic acquisition of crop morphology is beneficial to real-time variable decision of precise spraying operations in fields.However,the existing spraying quantity regulation has high tolerance on the statistical characteristics of regional morphology,so expensive LiDAR and ultrasonic radar can’t make full use of their high accuracy,and can reduce decision speed because of too much detail of branches and leaves.Therefore,designing a novel recognition system embedded machine learning with low-cost monocular vision is more feasible,especially in China,where the agricultural implements are medium sizes and cost-sensitive.In addition,we found that the growth period of crops is an important reference index for guiding spraying.So,taking cotton as a case study,a cotton morphology acquisition by a single camera is established,and a cotton growth period recognition algorithm based on Convolution Neural Network(CNN)is proposed in this paper.Through the optimization process based on confusion matrix and recognition efficiency,an optimized CNN model structure is determined from 9 different model structures,and its reliability was verified by changing training sets and test sets many times based on the idea of kfold test.The accuracy,precision,recall,F1-score and recognition speed of this CNN model are 93.27%,95.39%,94.31%,94.76%and 71.46 ms per image,respectively.In addition,compared with the performance of VGG16 and AlexNet,the convolution neural network model proposed in this paper has better performance.Finally,in order to verify the reliability of the designed recognition system and the feasibility of the spray decision-making algorithm based on CNN,spraying deposition experiments were carried out with 3 different growthperiods of cotton.The experiments’results validate that after the optimal spray parameters were applied at different growth periods respectively,the average optimum index in 3 growth periods was 42.29%,which was increased up to 62.24%than the operations without distinguishing growth periods.展开更多
Background:Recently,due to the development of food security strategies,cotton has been planted in inland saline-alkali dry soils or in coastal some saline-alkali soils in China.Under the condition,to comprehensively p...Background:Recently,due to the development of food security strategies,cotton has been planted in inland saline-alkali dry soils or in coastal some saline-alkali soils in China.Under the condition,to comprehensively prevent and control Helicoverpa armigera in cotton fields with saline-alkali soils,it is important to study the larval growth and development of H.armigero and to study adult oviposition selectivity in H.armigera adults that feed on NaCI-stNaCled cotton plants.Results:In this study,Bt cotton GK19 was used for the experimental group and its nontransgenic parent Simian 3 was used for the control to study the effects of biochemical substances in cotton as well as larval growth and development and adult oviposition selectivity of H.armigera.The experiments were performed by growing cotton indoors under NaCl stress at concentrations of 0 mmol-L-1,75 mmol-L-1 and 150 mmol-L-1,respectively.The results showed that the expression of Bt protein was significantly inhibited for NaCI-stressed Bt cotNaClThe content of soluble protein and K+ in the leaves of cotton were decreased,while the content of gossypol and Na+were increased.In addition,the 5th instar H.armigera larvae exhibited shorten the life span in a 13-day trial period.Under enclosure treatments and at different female densities,the adult oviposition of H.armigera decreased on high NaCI-stressed nontransgenic coNaCl,while the oviposition on Bt cotton tended to first increase but then decrease under low,moderate and high NaCl stress treatments.Conclusions:Under certain content ranges of NaCl stress treatments,larval of H.armigera growth and development,and adult oviposition were no significant difference in the change for a certain period.However,under high NaCl stress,larval growth,development and adult oviposition were affected,which may provide insights for the prevention and control of H.armigero for Bt cotton in saline-alkali soils.展开更多
Salinity is one of the most serious growth limiting factor, therefore, no longer being ignored. Although, cotton is fairly salt tolerant;its sensitivity at crop stand and yield is affected. This study is planned to id...Salinity is one of the most serious growth limiting factor, therefore, no longer being ignored. Although, cotton is fairly salt tolerant;its sensitivity at crop stand and yield is affected. This study is planned to identify the growth, physiological and molecular parameters in local cotton varieties FDH 171 and FDH 786 under NaCl stress. There was 100% seed germination but hypocotyl length was reduced at increasing level of NaCl. Plant height, fresh and dry biomass were reduced as the plants were subjected to increased stress of NaCl. Stomatal conductance, transpiration and photosynthetic rate and ionic imbalance were found to be reduced under the gradual increase in NaCl stress and affected the plant’s overall physiological processes. PCR product of AtNHX3 has been identified in stressed and non-stressed plants. Thus, the genotypes FDH 171 & FDH 786 were found tolerant to adoption of salt stress and could be used as a source in crop improvement.展开更多
The S-endotoxin genes of Bacillus thuringiensis (Bt) and proteinase inhibitor (PI) genes are two kinds of genes popularly used for developing transgenic plants resistant to insect pests. To clarify whether there is an...The S-endotoxin genes of Bacillus thuringiensis (Bt) and proteinase inhibitor (PI) genes are two kinds of genes popularly used for developing transgenic plants resistant to insect pests. To clarify whether there is any risk concerning the effects of pollens from these transgenic crops on non-target insects with economic importance, such as the effects on the growth and development as well as cocoon quality of the silkworm, Bombyx mori Linnaeus, a series of feeding experiments were conducted, using pollens from transgenic cotton or corn containing cry 1Ac, cry1A+CpTI or crylAb genes, compared with pollens from non-transgenic normal cotton and corn as well as the non-pollen treatment. In contrast to the latter ones, pollens from transgenic plants showed no significant adverse effects on larval mortality, cocoon weight, pupa weight, cocoon shell weight, pupation rate, emergence rate and fecundity of the silkworm after neonates were fed with the pollens for 72 h. In addition, no dosage effects of pollens were found. Though the duration of 1st instar larvae was prolonged in the case of feeding with transgenic pollens as compared with those of the non-pollen treatment , but they were not significantly different from those fed with pollens from non-transgenic cotton or corn. Meanwhile, the body weight of the 3rd instar molters fed with transgenic pollens was obviously different from those for non-pollen treatment, and was all significantly heavier than that of the controls. Consequently, it is considered that the adverse effect of pollens from transgenic insect-resistant cotton and corn on the growth and development of the silkworm is negligible.展开更多
[Objective] This study was to reveal the differences in two terminal oxdiases,so as to provide theoretical basis for the selection of upland and sea-land hybrid materials. [Methods] Using 7 shares of upland cotton cul...[Objective] This study was to reveal the differences in two terminal oxdiases,so as to provide theoretical basis for the selection of upland and sea-land hybrid materials. [Methods] Using 7 shares of upland cotton cultivars and 4 shares of sea-land cultivars as experimental materials,the change differences in AAO and PPO activities during whole growth period of the materials tested were studied. [Results] Two terminal oxdiases both in upland and sea-land cotton cultivars assumed similar change laws during whole growth period. AAO activity assumed a low-high trend,and there is a peak at the early stage. PPO activity presented two peaks,and that at the early stage was higher than at the late stage; the first peak is obviously higher than the second for sea-land cultivars,and two peaks are not remarkably different for upland cultivars. [Conclusion] The differences in AAO and PPO activities of sea-land and upland cotton cultivars observed on Jul.4th and Aug.6th,may provide guidance for the selection of upland and sea-land hybrids.展开更多
Cotton is the main economically important crop in Xinjiang,China,but soil salinization and shortage of water and nutrients have restricted its production.A field experiment was carried out in the salinity-affected ari...Cotton is the main economically important crop in Xinjiang,China,but soil salinization and shortage of water and nutrients have restricted its production.A field experiment was carried out in the salinity-affected arid area of Northwest China from 2018 to 2019 to explore the effects of nitrogen and water regulation on physiological growth,yield,water and nitrogen use efficiencies,and economic benefit of cotton.The salinity levels were 7.7(SL)and 12.5 dS/m(SM).Drip irrigation was used with low,medium and adequate irrigation levels representing 60%,80%and 100%of cotton crop water demand,respectively,and three nitrogen applications,i.e.,206,275 and 343 kg/hm^(2),accounting for 75%,100%and 125%of local N application,respectively were used.The multi-objective optimization based on spatial analysis showed that,at SL salinity,water use efficiency(WUE),nitrogen use efficiency(NUE),economic benefit and yield simultaneously reached more than 85%of their maxima at 379.18-398.32 mm irrigation and 256.69-308.87 kg/hm^(2).At SM salinity,WUE,yield and economic benefit simultaneously reached more than 85%of their maxima when irrigation was 351.24-376.30 mm and nitrogen application was 230.18-289.89 kg/hm^(2).NUE,yield and economic benefit simultaneously reached their maxima at 428.01-337.72 mm irrigation,and nitrogen application range was 222.14-293.93 kg/hm^(2).The plants at SL salinity had 21.58%-46.59%higher WUE rates,14.91%-34.35%higher NUE rates and 20.71%-35.34%higher yields than those at SM salinity.The results are of great importance for the nutrient and water management in cotton field in the arid saline area.展开更多
基金Financial support by UGC Research Fellowship(F.4-1/2006(BSR)to Dehariya P.)Department of Science Technology Women Scientists-A Scheme(SR/WOS-A/LS-674/2013-2016) to Kataria S.
文摘Background: Earlier we have reported that the exclusion of solar UV-B increased the growth and yield of cotton plants as compared with ambient UV-B. The UV-B radiation effects on the saponins and the impact of ambient and reduced UV-B on the accumulation of saponins has not been investigated yet. Thus a field experiment was conducted to study the influence of solar UV-B on the growth response and saponin synthesis in cotton(Gossypium hirsutum) var. Vikram plants by the exclusion of UV-B. The cotton plants were grown in specially designed iron chambers, wrapped with filters that excluded UV-B(<315 nm), or transmitted ambient UV-B.Results: Exclusion of ambient UV-B enhanced the growth of cotton plants in terms of plant height and leaf area.Greater plant height in UV-B excluded plants was due to elongated internode and more number of nodes.Enhancement in growth was accompanied by a decrease in the accumulation of saponins, which was quantified by the spectrophotometric, TLC and HPLC methods. Solvent extraction of saponins from the internodes and leaves were used for the bioassay of Amaranthus hypocotyl growth and expansion of cucumber cotyledons. Extracts obtained from the UV-B excluded plants(leaves and internodes) promoted the growth to a larger extent as compared with the extracts obtained from ambient grown plants.Conclusions: The result indicates that solar UV-B may possibly suppress the plant growth by regulating the synthesis of natural growth inhibitor’s like saponin.
基金supported by National Natural Science Foundation of China(51475278)China Shandong Province Agricultural Machinery Equipment Research and Development Innovation Project(2018YF002)+2 种基金China Natural Science Foundation of Shandong Province(ZR2019PC024)China Scientific Research and Development Projects of Universities in Shandong Province(J18KA128)China and the Funds of Shandong‘Double Tops’Program(SYL2017XTTD14),China.
文摘Dynamic acquisition of crop morphology is beneficial to real-time variable decision of precise spraying operations in fields.However,the existing spraying quantity regulation has high tolerance on the statistical characteristics of regional morphology,so expensive LiDAR and ultrasonic radar can’t make full use of their high accuracy,and can reduce decision speed because of too much detail of branches and leaves.Therefore,designing a novel recognition system embedded machine learning with low-cost monocular vision is more feasible,especially in China,where the agricultural implements are medium sizes and cost-sensitive.In addition,we found that the growth period of crops is an important reference index for guiding spraying.So,taking cotton as a case study,a cotton morphology acquisition by a single camera is established,and a cotton growth period recognition algorithm based on Convolution Neural Network(CNN)is proposed in this paper.Through the optimization process based on confusion matrix and recognition efficiency,an optimized CNN model structure is determined from 9 different model structures,and its reliability was verified by changing training sets and test sets many times based on the idea of kfold test.The accuracy,precision,recall,F1-score and recognition speed of this CNN model are 93.27%,95.39%,94.31%,94.76%and 71.46 ms per image,respectively.In addition,compared with the performance of VGG16 and AlexNet,the convolution neural network model proposed in this paper has better performance.Finally,in order to verify the reliability of the designed recognition system and the feasibility of the spray decision-making algorithm based on CNN,spraying deposition experiments were carried out with 3 different growthperiods of cotton.The experiments’results validate that after the optimal spray parameters were applied at different growth periods respectively,the average optimum index in 3 growth periods was 42.29%,which was increased up to 62.24%than the operations without distinguishing growth periods.
基金financial support from the National Natural Science Foundation of China(31501253)
文摘Background:Recently,due to the development of food security strategies,cotton has been planted in inland saline-alkali dry soils or in coastal some saline-alkali soils in China.Under the condition,to comprehensively prevent and control Helicoverpa armigera in cotton fields with saline-alkali soils,it is important to study the larval growth and development of H.armigero and to study adult oviposition selectivity in H.armigera adults that feed on NaCI-stNaCled cotton plants.Results:In this study,Bt cotton GK19 was used for the experimental group and its nontransgenic parent Simian 3 was used for the control to study the effects of biochemical substances in cotton as well as larval growth and development and adult oviposition selectivity of H.armigera.The experiments were performed by growing cotton indoors under NaCl stress at concentrations of 0 mmol-L-1,75 mmol-L-1 and 150 mmol-L-1,respectively.The results showed that the expression of Bt protein was significantly inhibited for NaCI-stressed Bt cotNaClThe content of soluble protein and K+ in the leaves of cotton were decreased,while the content of gossypol and Na+were increased.In addition,the 5th instar H.armigera larvae exhibited shorten the life span in a 13-day trial period.Under enclosure treatments and at different female densities,the adult oviposition of H.armigera decreased on high NaCI-stressed nontransgenic coNaCl,while the oviposition on Bt cotton tended to first increase but then decrease under low,moderate and high NaCl stress treatments.Conclusions:Under certain content ranges of NaCl stress treatments,larval of H.armigera growth and development,and adult oviposition were no significant difference in the change for a certain period.However,under high NaCl stress,larval growth,development and adult oviposition were affected,which may provide insights for the prevention and control of H.armigero for Bt cotton in saline-alkali soils.
文摘Salinity is one of the most serious growth limiting factor, therefore, no longer being ignored. Although, cotton is fairly salt tolerant;its sensitivity at crop stand and yield is affected. This study is planned to identify the growth, physiological and molecular parameters in local cotton varieties FDH 171 and FDH 786 under NaCl stress. There was 100% seed germination but hypocotyl length was reduced at increasing level of NaCl. Plant height, fresh and dry biomass were reduced as the plants were subjected to increased stress of NaCl. Stomatal conductance, transpiration and photosynthetic rate and ionic imbalance were found to be reduced under the gradual increase in NaCl stress and affected the plant’s overall physiological processes. PCR product of AtNHX3 has been identified in stressed and non-stressed plants. Thus, the genotypes FDH 171 & FDH 786 were found tolerant to adoption of salt stress and could be used as a source in crop improvement.
文摘The S-endotoxin genes of Bacillus thuringiensis (Bt) and proteinase inhibitor (PI) genes are two kinds of genes popularly used for developing transgenic plants resistant to insect pests. To clarify whether there is any risk concerning the effects of pollens from these transgenic crops on non-target insects with economic importance, such as the effects on the growth and development as well as cocoon quality of the silkworm, Bombyx mori Linnaeus, a series of feeding experiments were conducted, using pollens from transgenic cotton or corn containing cry 1Ac, cry1A+CpTI or crylAb genes, compared with pollens from non-transgenic normal cotton and corn as well as the non-pollen treatment. In contrast to the latter ones, pollens from transgenic plants showed no significant adverse effects on larval mortality, cocoon weight, pupa weight, cocoon shell weight, pupation rate, emergence rate and fecundity of the silkworm after neonates were fed with the pollens for 72 h. In addition, no dosage effects of pollens were found. Though the duration of 1st instar larvae was prolonged in the case of feeding with transgenic pollens as compared with those of the non-pollen treatment , but they were not significantly different from those fed with pollens from non-transgenic cotton or corn. Meanwhile, the body weight of the 3rd instar molters fed with transgenic pollens was obviously different from those for non-pollen treatment, and was all significantly heavier than that of the controls. Consequently, it is considered that the adverse effect of pollens from transgenic insect-resistant cotton and corn on the growth and development of the silkworm is negligible.
基金Supported by Sub-project of Key Project in the National Science &Technology Pillar Program in the Eleventh Five-year Plan Period(2006BAD01A05-27)the Starting Program for High-level Talents in Henan Institute of Science and Technology (6009)~~
文摘[Objective] This study was to reveal the differences in two terminal oxdiases,so as to provide theoretical basis for the selection of upland and sea-land hybrid materials. [Methods] Using 7 shares of upland cotton cultivars and 4 shares of sea-land cultivars as experimental materials,the change differences in AAO and PPO activities during whole growth period of the materials tested were studied. [Results] Two terminal oxdiases both in upland and sea-land cotton cultivars assumed similar change laws during whole growth period. AAO activity assumed a low-high trend,and there is a peak at the early stage. PPO activity presented two peaks,and that at the early stage was higher than at the late stage; the first peak is obviously higher than the second for sea-land cultivars,and two peaks are not remarkably different for upland cultivars. [Conclusion] The differences in AAO and PPO activities of sea-land and upland cotton cultivars observed on Jul.4th and Aug.6th,may provide guidance for the selection of upland and sea-land hybrids.
基金The study was supported by the National Natural Science Foundation of China(U1803244,51669029,2020DB01)the National Key Research and Development Program of China(2016YFC0501406).
文摘Cotton is the main economically important crop in Xinjiang,China,but soil salinization and shortage of water and nutrients have restricted its production.A field experiment was carried out in the salinity-affected arid area of Northwest China from 2018 to 2019 to explore the effects of nitrogen and water regulation on physiological growth,yield,water and nitrogen use efficiencies,and economic benefit of cotton.The salinity levels were 7.7(SL)and 12.5 dS/m(SM).Drip irrigation was used with low,medium and adequate irrigation levels representing 60%,80%and 100%of cotton crop water demand,respectively,and three nitrogen applications,i.e.,206,275 and 343 kg/hm^(2),accounting for 75%,100%and 125%of local N application,respectively were used.The multi-objective optimization based on spatial analysis showed that,at SL salinity,water use efficiency(WUE),nitrogen use efficiency(NUE),economic benefit and yield simultaneously reached more than 85%of their maxima at 379.18-398.32 mm irrigation and 256.69-308.87 kg/hm^(2).At SM salinity,WUE,yield and economic benefit simultaneously reached more than 85%of their maxima when irrigation was 351.24-376.30 mm and nitrogen application was 230.18-289.89 kg/hm^(2).NUE,yield and economic benefit simultaneously reached their maxima at 428.01-337.72 mm irrigation,and nitrogen application range was 222.14-293.93 kg/hm^(2).The plants at SL salinity had 21.58%-46.59%higher WUE rates,14.91%-34.35%higher NUE rates and 20.71%-35.34%higher yields than those at SM salinity.The results are of great importance for the nutrient and water management in cotton field in the arid saline area.