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Genetic Map of Cotton with Molecular Markers
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作者 Camila Campelo de Sousa Livio Carvalho de Figueiredo +1 位作者 Maria das Gracas Medina Arrais Sergio Emilio dos Santos Valente 《American Journal of Plant Sciences》 2015年第2期289-297,共9页
Cotton (Gossypium spp.) is the most important natural fiber in the world, and its seeds are also used as a food source. Breeding cotton for traits of interest, such as production and processing of fibers, will ensure ... Cotton (Gossypium spp.) is the most important natural fiber in the world, and its seeds are also used as a food source. Breeding cotton for traits of interest, such as production and processing of fibers, will ensure that this natural product is as competitive as renewable synthetic fibers derived from petroleum. Thus, the mapping of the cotton genome for traits of interest may be the basis for its subsequent use in breeding programs. This work consists of a literature review, with the aim of bringing together works from different research groups working with the mapping of the cotton genome with molecular markers. 展开更多
关键词 cotton Genome Genetic Map GOSSYPIUM molecular markers Quantitative Trait Loci
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QTL Mapping for Fiber Quality Traits Based on a Dense Genetic Linkage Map with SSR,TRAP,SRAP and AFLP Markers in Cultivated Tetraploid Cotton 被引量:1
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作者 YU Ji-wen1,YU Shu-xun1,ZHANG Jin-fa2,ZHAI Hong-hong1(1.Cotton Research Institute of CAAS Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China 2.Department of Plant and Environmental Sciences,New Mexico State University,Las Cruces,NM 88003) 《棉花学报》 CSCD 北大核心 2008年第S1期34-,共1页
Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,b... Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,both cotton yield and quality should be enhanced.However,cotton yield 展开更多
关键词 QTLs AFLP QTL Mapping for Fiber Quality Traits Based on a Dense Genetic linkage Map with SSR TRAP SRAP and AFLP markers in Cultivated Tetraploid cotton SSR Map
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Constructing Molecular Marker Linkage Maps of Chromosome 14Sh and 22Sh and QTL Mapping for Major Traits by Use of Substitution Lines of Gossypium hirsutum L.
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作者 SAHA Sukumar JENKINS Johnie N 《棉花学报》 CSCD 北大核心 2008年第S1期85-,共1页
CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used ... CSB14Sh,which is isogenic for its recurrent parent TM-1 except for chromosome 14 short arm,was crossed with TM-1,and the F2 population was produced.A total of 3800 SSR primer pairs covering the whole genome were used to screen polymorphism among two parents,TM-1 and CSB14Sh, 展开更多
关键词 QTLs Constructing molecular Marker linkage maps of Chromosome 14Sh and 22Sh and QTL Mapping for Major Traits by Use of Substitution Lines of Gossypium hirsutum L
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Simple Sequence Repeat Genetic Linkage Maps of A-genome Diploid Cotton (Gossypium arboreum) 被引量:4
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作者 Xue-Xia Ma Bao-Liang Zhou Yan-Hui Lü Wang-Zhen Guo Tian-Zhen Zhang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2008年第4期491-502,共12页
This study introduces the construction of the first intraspecific genetic linkage map of the A-genome diploid cotton with newly developed simple sequence repeat (SSR) markers using 189 F2 plants derived from the cro... This study introduces the construction of the first intraspecific genetic linkage map of the A-genome diploid cotton with newly developed simple sequence repeat (SSR) markers using 189 F2 plants derived from the cross of two Asiatic cotton cultivars (Gossypium arboreum L.) Jianglingzhongmlan x Zhejiangxiaoshanl(ishu. Polymorphisms between the two parents were detected using 6 092 pairs of SSR primers. Two-hundred and sixty-eight pairs of SSR primers with better polymorphisms were picked out to analyze the F2 population. In total, 320 polymorphic bands were generated and used to construct a linkage map with JoinMap3.0. Two-hundred and sixty-seven loci, including three phenotypic traits were mapped at a logarithms of odds ratio (LOD)≥ 3.0 on 13 linkage groups. The total length of the map was 2 508.71 cM, and the average distance between adjacent markers was 9.40 cM. Chromosome assignments were according to the association of linkages with our backbone tetraploid specific map using the 89 similar SSR loci. Comparisons among the 13 suites of orthologous linkage groups revealed that the A-genome chromosomes are largely collinear with the At and Dt sub- genome chromosomes. Chromosomes associated with inversions suggested that allopolyploidization was accompanied by homologous chromosomal rearrangement. The inter-chromosomal duplicated loci supply molecular evidence that the A-genome diploid Asiatic cotton is paleopolyploid. 展开更多
关键词 DIPLOID genetic linkage map Gossypium arboreum molecular marker simple sequence repeat.
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Identification of Quantitative Trait Loci (QTLs) Affecting Yield and Fiber Properties in Chromosome 16 in Cotton Using Substitution Line 被引量:12
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作者 任立华 郭旺珍 张天真 《Acta Botanica Sinica》 CSCD 2002年第7期815-820,共6页
Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substit... Gossypium hirsutum L. and G. barbadense L. are the two cultivated tetraploid species of cotton. The first is characterized by a high yield and wide adaptation, and the second by its super fiber property. Substitution line in which a pair of intact chromosomes of TM_1 ( G. hirsutum ) were replaced by a pair of homozygous chromosomes of 3_79 ( G. barbadense ) is an excellent material for genetic research and molecular tagging. In this study, substitution line 16 (Sub 16) was used to evaluate the performance of the 16th chromosome in G. barbadense in TM_1 background. The genetic analysis using the major gene plus polygene mixed inheritance model in F 2∶3 family revealed that there might exist 2 QTLs respectively for boll size, lint percentage, lint index, fiber length and the first fruit branch node, 1 QTL for fiber elongation and flowering date, and no QTL for seed index, fiber strength and Micronaire in chromosome 16. However, 9 QTLs (LOD (logarithm of odds)≥3.0) controlling 6 quantitative traits were significantly identified in linkage group of chromosome 16 constructed in (TM_1×3_79) F 2by interval mapping. Among them, 1 QTL for boll size, fiber length, flowering date and fiber elongation could explain 15.2%, 19.7%, 12.1%, and 11.7% phenotypic variance respectively, 2 QTLs for lint index could explain 11.6% and 41.9%, and 3 QTLs for lint percentage could explain 8.7%, 9.6% and 29.2% phenotypic variance respectively. One unlinked SSR marker was associated with one QTL respectively for boll size and flowering date and they could explain 1.60% and 4.63% phenotypic variance. The traits associated significantly with chromosome 16 from Sub 16 were boll weight, lint percentage, lint index, fiber length, fiber elongation and flowering days. 展开更多
关键词 allotetraploid cotton substitution line chromosome 16 inheritance molecular marker QTL mapping
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Molecular Tagging and Mapping of Quantitative Trait Loci for Lint Percentage and Morphological Marker Genes in Upland Cotton 被引量:15
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作者 Wang-Zhen Guo Guo-Jia Ma Yi-Chao Zhu Chen-Xin Yi Tian-Zhen Zhang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第3期320-326,共7页
Using 219 F2 Individuals developed by crossing the genetic standard line TM-1 and the multiple dominant marker line T586 In Gossyplum hirsutum L., a genetic linkage map with 19 linkage groups was constructed based on ... Using 219 F2 Individuals developed by crossing the genetic standard line TM-1 and the multiple dominant marker line T586 In Gossyplum hirsutum L., a genetic linkage map with 19 linkage groups was constructed based on simple sequence repeat (SSR) markers. Compared with our tetraploid backboned molecular genetic map from a (TM-1xHal 7124)xTM-1 BC1 population, 17 of the 19 I|nkage groups were combined and anchored to 12 chromosomes (sub-genomes). Of these groups, four morphological marker genes In T586 had been mapped Into the molecular linkage map. Meanwhile, three quantitative trait loci for lint percentage were tagged and mapped separately on the A03 linkage group and chromosome 6. 展开更多
关键词 genetic linkage map association morphological markers quantitative trait locus tagging upland cotton.
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Construction of a genetic linkage map for cotton based on SRAP 被引量:111
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作者 LIN Zhongxu, ZHANG Xianlong, NIE Yichun, HE Daohua & WU Maoqing National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China 《Chinese Science Bulletin》 SCIE EI CAS 2003年第19期2063-2067,共5页
A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific... A genetic linkage map of cotton was constructed with a newly developed molecular marker-SRAP (sequence-related amplified polymorphism) using a population consisting of 129 F2 individuals derived from the interspecific cross of Handan208 Pima90. A total of 136 primer pairs were used to detect polymorphisms between the two parents and 76 primer pairs with better polymorphisms were picked out to analyze the F2 population. 285 polymorphic bands were generated in total with an average of 3.75 polymorphic bands per pair of primers. The primer pair showing most polymorphic bands was the combination of me3 and em2, which produced 13 polymorphic bands. The 285 loci were used to construct linkage map with MAPMAKER/EXP3.0 and 237 loci were mapped at a LOD≥3.0 on 39 linkage groups. The total length of the map is 3030.7 cM, covering 65.4% of the whole cotton genome, and the average distance between adjacent markers is 12.79 cM. All the markers are distributed evenly among the linkage groups without clustering of loci. This is the first linkage map of cotton comprised of SRAP markers. 展开更多
关键词 SRAP 棉花 遗传连锁图 序列相关扩大多态性 分子标记 DNA
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Construction of a linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.) 被引量:7
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作者 LIANG QingZhi HU Cheng +2 位作者 HUA Hua LI ZhaoHu HUA JinPing 《Chinese Science Bulletin》 SCIE EI CAS 2013年第26期3233-3243,共11页
With the development in spinning technology, the improvement of cotton fiber quality is becoming more and more important. The main objective of this research was to construct a high-density genetic linkage map to faci... With the development in spinning technology, the improvement of cotton fiber quality is becoming more and more important. The main objective of this research was to construct a high-density genetic linkage map to facilitate marker assisted selection for fiber quality traits in upland cotton (Gossypium hirsutum L.). A genetic linkage map comprising 421 loci and covering 3814.3 cM, accounting for approximately 73.35% of the cotton genome, was constructed using an F2 population derived from cross GX1135 (P 1 )×GX100-2 (P 2 ). Forty-four of 49 linkage groups were assigned to the 26 chromosomes. Fiber quality traits were investigated in F2 population sampled from individuals, and in F2:3 , and F2:4 generations sampled by lines from two sites and one respectively, and each followed a randomized complete block design with two replications. Thirty-nine quantitative trait loci were detected for five fiber quality traits with data from single environments (separate analysis each): 12 for fiber length, five for fiber uniformity, nine for fiber strength, seven for fiber elongation, and six for fiber micronaire, whereas 15 QTLs were found in combined analysis (data from means of different environments in F2:3 generation). Among these QTLs, qFL-chr5-2 and qFL-chr14-2 for fiber length were detected simultaneously in three generations (four environments) and verified further by combined analysis, and these QTLs should be useful for marker assisted selection to improve fiber quality in upland cotton. 展开更多
关键词 纤维品质性状 遗传连锁图谱 QTL定位 陆地棉 分子标记辅助选择 F2群体 随机区组设计 数量性状位点
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Status and Advances of Molecular Genetic Improvement of Poplar Species in China 被引量:1
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作者 张志毅 林善枝 张谦 《Forestry Studies in China》 CAS 2002年第2期1-8,共8页
Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many use... Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many uses, easiness of tissue culture and small gene group that make them well suited as a model system for the application of genetic engineering in forest trees. In the last decade, much progress has been made in genetic improvement of poplar species in China. Modern biotechnology is an important tool for genetic improvement in forest trees, and its applications to genetic improvement in poplars, which covers genetic transformation, gene expression, construction of genetic linkage map, QTLs (quantitative trait loci) identification and molecular assisted selection are reviewed in this paper. At the same time, the existing problems and outlook about the application of modern biotechnology to genetic improvement in forest trees are also discussed. 展开更多
关键词 POPLAR gene engineering molecular marker genetic linkage maps QTLS genetic improvement
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Fine mapping of the dominant glandless Gene Gl_2~e in Sea-island cotton (Gossypium barbadense L.) 被引量:12
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作者 DONG ChengGuang DING YeZhang GUO WangZhen ZHANG TianZhen 《Chinese Science Bulletin》 SCIE EI CAS 2007年第22期3105-3109,共5页
Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly ... Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gle2 by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gle2 was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum × G. barbadense BC1 published by our laboratory,Gle2 was lo-cated between CIR362 and NAU2251b,NAU3860b,STV033,with a genetic distance 9.27 and 0.96 cM,respectively. This result is useful for cloning Gle2 gene by map-based cloning method. 展开更多
关键词 显性 突变基因 棉花 种子
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棉花功能基因图位克隆的研究进展 被引量:1
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作者 臧新山 王康文 +10 位作者 张先亮 王雪平 王军 梁雨 裴小雨 任翔 吕宇龙 高宇 王星星 彭云玲 马雄风 《中国农业科学》 CAS CSCD 北大核心 2023年第23期4635-4647,共13页
图位克隆是鉴定特定表型变异遗传基础的经典有效策略。棉花功能基因图位克隆,对育种工作者创新利用种质资源、培育和定向设计新品种、提高育种效率有重要指导作用。近年来,随着雷蒙德氏棉、亚洲棉、陆地棉和海岛棉等基因组测序的完成和... 图位克隆是鉴定特定表型变异遗传基础的经典有效策略。棉花功能基因图位克隆,对育种工作者创新利用种质资源、培育和定向设计新品种、提高育种效率有重要指导作用。近年来,随着雷蒙德氏棉、亚洲棉、陆地棉和海岛棉等基因组测序的完成和不断完善,基因的物理位置信息已知,省去了筛选基因组文库和构建候选区段物理图谱的过程,棉花功能基因图位克隆研究进入快速发展期。2016年,利用正向遗传学方法首次图位克隆了陆地棉显性无腺体Gl2e(GoPGF),目前已有20个质量性状基因和5个数量性状基因通过图位克隆策略鉴定。本文从基因符号、名称、染色体定位、候选基因等方面系统综述棉花纤维、腺体、蜜腺、叶型、株型、植株颜色、育性等性状相关图位克隆基因;并从图位克隆作图群体和集团分离分析法测序(bulked segregate analysis-sequencing,BSA-seq)应用等方面系统综述图位克隆策略。随着基因组测序技术的升级、测序成本的降低、BSA-seq等新方法的应用,图位克隆发展更加快速准确。利用转基因和基因组编辑技术对基因功能开展全面系统的鉴定评价,将为棉花分子设计育种提供理论基础和基因资源,加快棉花遗传改良进程。 展开更多
关键词 棉花 图位克隆 分子标记 作图群体 BSA-seq
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棉花窄卷苞叶基因fg的精细定位
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作者 王康文 王雪平 +8 位作者 王军 梁雨 裴小雨 任翔 王星星 张先亮 彭云玲 臧新山 马雄风 《棉花学报》 CSCD 北大核心 2023年第3期173-180,共8页
【目的】窄卷苞叶可以减少棉花苞叶碎屑的附着,有助于降低机采棉含杂率。对棉花窄卷苞叶基因fg进行精细定位,为该基因的图位克隆和育种利用提供参考。【方法】以陆地棉T582为母本,分别与父本陆地棉TM-1、海岛棉3-79杂交构建2个F2分离群... 【目的】窄卷苞叶可以减少棉花苞叶碎屑的附着,有助于降低机采棉含杂率。对棉花窄卷苞叶基因fg进行精细定位,为该基因的图位克隆和育种利用提供参考。【方法】以陆地棉T582为母本,分别与父本陆地棉TM-1、海岛棉3-79杂交构建2个F2分离群体;其中群体1(T582×TM-1)包含370个单株,群体2(T582×3-79)包含2667个单株。根据TM-1和3-79参考基因组数据,利用开发的插入缺失(insertion-deletion,Indel)标记对fg进行精细定位。利用棉花功能基因组学和多组学数据,对定位区间内的基因进行功能注释及表达模式分析。【结果】遗传分析结果表明,棉花窄卷苞叶由隐性单基因控制。在前人对fg基因初定位的基础上,进一步将窄卷苞叶基因fg定位在A03染色体分子标记M3与M4之间,区间大小为188 kb。预测定位区间内有14个注释的功能基因。其中,Gh_A03G021700、Gh_A03G021900、Gh_A03G022600和Gh_A03G022700基因在花萼、副萼中的表达量较高。【结论】棉花窄卷苞叶fg基因被精细定位在A03染色体188 kb区间内,并初步分析了定位区间内的14个候选基因,为该基因的图位克隆奠定了基础。 展开更多
关键词 棉花 窄卷苞叶 FG 分子标记 精细定位 F2群体
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分子标记在蚕豆遗传育种中的研究进展
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作者 张兴民 池小娜 +2 位作者 顾文媛 邵扬 王玉萍 《中国蔬菜》 北大核心 2023年第9期31-37,共7页
蚕豆自然异交率高造成群体内杂合度较高,通过传统的田间表型观察难以区分遗传变异,分子标记为蚕豆种质资源鉴定与聚类分析提供了新方法。本文基于分子标记类型及发展,对国内外利用分子标记在蚕豆遗传连锁图谱构建、分子辅助选择、种质... 蚕豆自然异交率高造成群体内杂合度较高,通过传统的田间表型观察难以区分遗传变异,分子标记为蚕豆种质资源鉴定与聚类分析提供了新方法。本文基于分子标记类型及发展,对国内外利用分子标记在蚕豆遗传连锁图谱构建、分子辅助选择、种质资源评价和基因定位方面的研究进展进行了分析总结,并探讨了分子标记在蚕豆遗传育种中的发展前景,以期为提高蚕豆种质资源利用效率和遗传改良提供参考。 展开更多
关键词 蚕豆 遗传图谱 分子标记辅助选择 种质资源评价 基因定位
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陆地棉产量性状QTLs的分子标记及定位 被引量:48
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作者 殷剑美 武耀廷 +3 位作者 张军 张天真 郭旺珍 朱协飞 《生物工程学报》 CAS CSCD 北大核心 2002年第2期162-166,共5页
应用我国的高产栽培品种泗棉 3号和美国栽培品种TM 1为材料 ,构建F2 和F2∶3 作图群体 ,应用 30 1对SSR引物和 10 40个RAPD引物 ,对产量性状QTLs进行了分子标记筛选 ,结果共筛选出了 37对SSR多态性引物和 10个RAPD多态性引物的 49个位... 应用我国的高产栽培品种泗棉 3号和美国栽培品种TM 1为材料 ,构建F2 和F2∶3 作图群体 ,应用 30 1对SSR引物和 10 40个RAPD引物 ,对产量性状QTLs进行了分子标记筛选 ,结果共筛选出了 37对SSR多态性引物和 10个RAPD多态性引物的 49个位点 ,鉴定出了控制产量性状变异的主效QTLs。定位于第 9染色体的连锁群 ,分别具有控制铃重、衣分和籽指的主效QTLs ,铃重的 2个QTLs分别解释F2∶3 群体表型变异的 18 2 %和 2 1 0 % ;在F2 群体检测到的 1个衣分QTL解释表型变异的 2 5 % ,另一个衣分QTL在F2 群体和F2∶3 群体都检测到 ,解释F2 群体衣分的2 4 9%的表型变异 ,解释F2∶3 群体衣分的 5 9%的表型变异 ;在F2∶3 群体铃重的一个QTL的同一位置同时检测到一个籽指QTL ,它解释 15 6 %的表型变异 ,是一因多效或是紧密连锁的两个QTLs,有待进一步研究。 展开更多
关键词 陆地棉 产量性状 QTL 分子标记 区间作图 定位
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棉花黄萎病抗性基因SSR标记研究 被引量:48
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作者 杜威世 杜雄明 马峙英 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2004年第3期20-24,共5页
 以高抗黄萎病的海岛棉品种" 15-3493"和高感黄萎病的陆地棉品种"石河子875"的175个F2代单株作为标记群体,采用BSA法筛选多态性引物,构建了1个包括3个SSR引物在内的连锁群。通过Mapmaker软件将与黄萎病抗性有关的...  以高抗黄萎病的海岛棉品种" 15-3493"和高感黄萎病的陆地棉品种"石河子875"的175个F2代单株作为标记群体,采用BSA法筛选多态性引物,构建了1个包括3个SSR引物在内的连锁群。通过Mapmaker软件将与黄萎病抗性有关的1个位点定位在该连锁群,该位点与SSR标记BNL3556相距13.1cM,可解释表型变异方差的50.1%,为主效基因位点。 展开更多
关键词 棉花 黄萎病 抗性基因 SSR标记 连锁群 分子标记辅助选择育种 抗病育种
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家蚕AFLP连锁框架图谱的构建 被引量:23
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作者 朱玉芳 谭远德 +4 位作者 万春玲 鲁成 贺一原 周泽扬 向仲怀 《昆虫学报》 CAS CSCD 北大核心 2001年第4期483-493,共11页
利用改进的AFLP分子标记方法 ,对家蚕Bombyxmori回交一代BC1群体进行连锁图谱的构建。经 17组AFLP引物的选择性扩增 ,共得到 4 30个多态位点 ,卡方检验后有 2 5 3个为有效位点。利用Mapmaker Exp (Version 3 0b)软件作连锁分析 ,其中 1... 利用改进的AFLP分子标记方法 ,对家蚕Bombyxmori回交一代BC1群体进行连锁图谱的构建。经 17组AFLP引物的选择性扩增 ,共得到 4 30个多态位点 ,卡方检验后有 2 5 3个为有效位点。利用Mapmaker Exp (Version 3 0b)软件作连锁分析 ,其中 16 3个标记分属 2 8个连锁群 ,连锁群标记数变化范围是 2~ 2 8个 ,平均每个连锁群标记数为 5 8个 ,该图覆盖的基因组长度为 2 998 9cM(图距单位 ) ,连锁群长度变化范围为 4 5~ 6 5 2 8cM ,连锁群的平均长度为 10 7 1cM ,平均图距为4 5~ 36 7cM。 展开更多
关键词 家蚕 分子标记 AFLP 连锁图谱
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利用SRAP标记构建甘薯分子连锁图谱 被引量:38
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作者 李爱贤 刘庆昌 +3 位作者 王庆美 张立明 翟红 刘树震 《作物学报》 CAS CSCD 北大核心 2010年第8期1286-1295,共10页
以高淀粉甘薯品种漯徐薯8号为母本,低淀粉甘薯品种郑薯20为父本,杂交得到的F1分离群体的240个单株为作图群体,利用SRAP标记技术,共得到770个母本的多态性标记和523个父本的多态性标记,用JoinMap3.0软件和"双假测交"策略,分别... 以高淀粉甘薯品种漯徐薯8号为母本,低淀粉甘薯品种郑薯20为父本,杂交得到的F1分离群体的240个单株为作图群体,利用SRAP标记技术,共得到770个母本的多态性标记和523个父本的多态性标记,用JoinMap3.0软件和"双假测交"策略,分别构建了2个亲本的分子连锁图谱。其中漯徐薯8号的图谱包括由473个SRAP标记组成的81个连锁群,总图距为5802.46cM,标记间平均图距为10.16cM;郑薯20的图谱包括由328个SRAP标记组成的66个连锁群,总图距为3967.90cM,标记间平均图距为12.02cM。该高密度分子连锁图谱的构建,为甘薯分子标记辅助选择、基因定位及克隆研究奠定了基础。 展开更多
关键词 甘薯 分子连锁图谱 SRAP标记
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棉花抗黄萎病基因的QTL定位 被引量:67
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作者 高玉千 聂以春 张献龙 《棉花学报》 CSCD 北大核心 2003年第2期73-78,共6页
以高感黄萎病的陆地棉品种"邯郸208"与高抗黄萎病海岛棉品种"Pima90"的136个F2单株为作图群体,构建了一个包括17个连锁群、标记间平均间距18.61cM、全长1842.8cM的陆海种间分子标记遗传连锁图,该图约覆盖棉花基因组... 以高感黄萎病的陆地棉品种"邯郸208"与高抗黄萎病海岛棉品种"Pima90"的136个F2单株为作图群体,构建了一个包括17个连锁群、标记间平均间距18.61cM、全长1842.8cM的陆海种间分子标记遗传连锁图,该图约覆盖棉花基因组的36.8%。单因子方差分析和复合区间作图检测到与黄萎病抗性相关的3个QTL,分别位于第四连锁群和第七连锁群上,分别解释表型变异方差的15.39%、54.11%和57.18%。初步认为海岛棉"Pima90"对陆地棉"邯郸208"的黄萎病抗性由两个主效QTL和一个微效QTL共同控制。 展开更多
关键词 棉花 黄萎病 分子标记遗传连锁图 QTL定位
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甘蓝型油菜产量及其构成因素的QTL定位与分析 被引量:27
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作者 张书芬 傅廷栋 +3 位作者 朱家成 王建平 文雁成 马朝芝 《作物学报》 CAS CSCD 北大核心 2006年第8期1135-1142,共8页
产量性状是复杂的数量性状,对种子的单株产量及其构成因素(全株总有效角果数、每角粒数、千粒重)进行QTL定位和上位性分析,确定其在染色体上的位置及其遗传效应,可以探讨油菜杂种优势产生原因,提高育种中对产量性状优良基因型选... 产量性状是复杂的数量性状,对种子的单株产量及其构成因素(全株总有效角果数、每角粒数、千粒重)进行QTL定位和上位性分析,确定其在染色体上的位置及其遗传效应,可以探讨油菜杂种优势产生原因,提高育种中对产量性状优良基因型选择的效率,达到提高油菜产量的目的。在双低油菜细胞质雄性不育保持系1141B和双高恢复系垦C1构建的F2作图群体中,运用SRAP、AFLP和SSR三种标记技术构建了一个甘蓝型油菜(Brassica napus L.)的分子标记遗传连锁图谱。共包含244个标记,分布于20个主要连锁群、1个三联体上,图谱总长度为2769.5cM。采用Windows QTL Cartographer Version 2.0统计软件及复合区间作图法,对油菜单株产量及其3大构成因素进行QTL定位,共检测到QTLs16个,分布在9个连锁群上,其中第6和13连锁群最多,均有3个。单个QTL解释性状表型变异的0.38%-73.34%。对于同一性状,等位基因的增效作用既来自母本,亦源自父本;采用双向方差分析法对位点间互作及其上位性进行分析,检测到26对影响产量构成性状的上位性互作效应QTL,说明油菜基因组中存在大量控制产量的互作位点,油菜产量性状的上位性存在着多效性,上位性互作包括QTL与非QTL位点,其中以非QTL位点较多。一般互作位点的独立效应值较小,而互作的效应值显著增大,且一般超过两位点独立效应值之和。反映了控制产量性状基因的复杂性。上位性是甘蓝型油菜产量性状杂种优势的重要遗传基础。 展开更多
关键词 甘蓝型油菜 产量构成因素 遗传图谱 分子标记 QTL定位 上位性
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茶树AFLP分子连锁图谱的构建 被引量:32
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作者 黄建安 李家贤 +3 位作者 黄意欢 罗军武 龚志华 刘仲华 《茶叶科学》 CAS CSCD 北大核心 2005年第1期7-15,共9页
采用改进的AFLP技术体系,对祁门4号×潮安大乌叶的F1代群体进行连锁图谱的构建。经22对引物组合的选择性扩增,共获得1925条带,平均每对引物产生87.5条带,获得多态性带485条,多态性带的比率为25.19%。共有356(73.40%)个多态性位点符... 采用改进的AFLP技术体系,对祁门4号×潮安大乌叶的F1代群体进行连锁图谱的构建。经22对引物组合的选择性扩增,共获得1925条带,平均每对引物产生87.5条带,获得多态性带485条,多态性带的比率为25.19%。共有356(73.40%)个多态性位点符合孟德尔分离比例(P=0.01),其中发生1:1分离的位点为247(69.38%)个,发生3:1分离的位点为109(30.62%)个。采用Mapmaker/Exp3.0软件进行连锁分析,分别构建了祁门4号与潮安大乌叶的AFLP分子连锁图谱,其中母本图谱包括由208个标记组成的17个连锁群,总图距为2457.7cM,标记间平均间距为11.9cM。父本图谱包括由200个标记组成的16个连锁群,总图距为2545.3cM,标记间平均间距为12.8cM。 展开更多
关键词 茶树 分子连锁图谱 AFLP
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