This study marks the birth of visible and selective click covalent assembly.It is achieved by amplifying orthogonal alkyne−azide click chemistry through interfacial multisite interactions between azide/alkyne function...This study marks the birth of visible and selective click covalent assembly.It is achieved by amplifying orthogonal alkyne−azide click chemistry through interfacial multisite interactions between azide/alkyne functionalized polymer hydrogels.Macroscopic assembly of hydrogels via host−guest chemistry or noncovalent interactions such as electrostatic interactions has been reported.Unlike macroscopic supramolecular assembly,here we report visible and selective“click”covalent assembly of hydrogels at the macroscale.LEGO-like hydrogels modified with alkyne and azide groups,respectively,can click together via the formation of covalent bonds.Monomer concentration-dependent assembly and selective covalent assembly have been studied.Notably,macroscopic gel assembly clearly elucidates click preferences and component selectivity not observed in the solution reactions of competing monomers.展开更多
Radical detection has attracted significant attention recently. Here we have developed a scaffold through covalent assembly principle（OR570）, which could facile applications in detection of oxidative radicals.The pr...Radical detection has attracted significant attention recently. Here we have developed a scaffold through covalent assembly principle（OR570）, which could facile applications in detection of oxidative radicals.The primary advantage of the assembly type probe lies at the turn-on fluorescence signal from a zero background and hence high fluorescence turn-on ratio for sensitive detection of weak signal.展开更多
A facile surface plasmon resonance(SPR) chip is developed for small molecule determination and analysis. The SPR chip was prepared based on a self assembling principle, in which the modified bovine serum albumin(BSA) ...A facile surface plasmon resonance(SPR) chip is developed for small molecule determination and analysis. The SPR chip was prepared based on a self assembling principle, in which the modified bovine serum albumin(BSA) was directly self-assembled onto the bare gold surface. The surface morphology of the chip with the modified BSA was investigated by atomic force microscopy(AFM) and its optical properties were characterized. The surface binding capacity of the bare facile SPR chip with a uniform morphology is 8 times of that of the bare control SPR chip. Based on the experiments of immune reaction between cortisol antibody and cortisol derivative, the sensitivity of the facile SPR chip with the modified BSA is much higher than that of the control SPR chip with the un-modified BSA. The facile SPR chip has been successfully used to detect small molecules. The lowest detection limit is 5 ng/m L with a linear range of 5—100 ng/m L for cortisol analysis. The novel facile SPR chip can also be applied to detect other small molecules.展开更多
基金Guangdong Provincial Pearl River Talents Program (No.2021ZT090195)Guangdong Basic and Applied Basic Research Foundation (No.2020A1515110777)Shenzhen Science,Technology and Innovation Committee (STIC)Shenzhen Key Laboratory Fund (No.ZDSYS20220507161600001)for the financial support for this work.
文摘This study marks the birth of visible and selective click covalent assembly.It is achieved by amplifying orthogonal alkyne−azide click chemistry through interfacial multisite interactions between azide/alkyne functionalized polymer hydrogels.Macroscopic assembly of hydrogels via host−guest chemistry or noncovalent interactions such as electrostatic interactions has been reported.Unlike macroscopic supramolecular assembly,here we report visible and selective“click”covalent assembly of hydrogels at the macroscale.LEGO-like hydrogels modified with alkyne and azide groups,respectively,can click together via the formation of covalent bonds.Monomer concentration-dependent assembly and selective covalent assembly have been studied.Notably,macroscopic gel assembly clearly elucidates click preferences and component selectivity not observed in the solution reactions of competing monomers.
基金supported by the Fundamental Research Funds for the Central Universities (Nos. 1222201718004, WY1514053 and WY1516017)the National Natural Science Foundation of China (Nos. 21372080, 21572061, and 21236002)
文摘Radical detection has attracted significant attention recently. Here we have developed a scaffold through covalent assembly principle（OR570）, which could facile applications in detection of oxidative radicals.The primary advantage of the assembly type probe lies at the turn-on fluorescence signal from a zero background and hence high fluorescence turn-on ratio for sensitive detection of weak signal.
基金supported by the National Basic Research Program of China(No.2014CB744600)the National High Technology Research and Development Program of China(No.2014AA022303)the National Natural Science Foundation of China(Nos.61571420,31571007,61201079,61372055 and 81371711)
文摘A facile surface plasmon resonance(SPR) chip is developed for small molecule determination and analysis. The SPR chip was prepared based on a self assembling principle, in which the modified bovine serum albumin(BSA) was directly self-assembled onto the bare gold surface. The surface morphology of the chip with the modified BSA was investigated by atomic force microscopy(AFM) and its optical properties were characterized. The surface binding capacity of the bare facile SPR chip with a uniform morphology is 8 times of that of the bare control SPR chip. Based on the experiments of immune reaction between cortisol antibody and cortisol derivative, the sensitivity of the facile SPR chip with the modified BSA is much higher than that of the control SPR chip with the un-modified BSA. The facile SPR chip has been successfully used to detect small molecules. The lowest detection limit is 5 ng/m L with a linear range of 5—100 ng/m L for cortisol analysis. The novel facile SPR chip can also be applied to detect other small molecules.
